TBLASTN 2.2.13 [Nov-27-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= SPP00000001_1.0 # Protein # Iodothyronine deiodinase 1 (DI1) # Homo sapiens # Complete (249 letters) Database: genome.fa 572 sequences; 14,903,514 total letters Searching...................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAQX01000438.1| Phytophthora ramorum strain Pr102 Pramorum_Co... 27 6.6 gb|AAQX01000569.1| Phytophthora ramorum strain Pr102 Pramorum_Co... 27 8.6 >gb|AAQX01000438.1| Phytophthora ramorum strain Pr102 Pramorum_Cont438, whole genome shotgun sequence Length = 107380 Score = 26.9 bits (58), Expect = 6.6 Identities = 23/86 (26%), Positives = 38/86 (44%), Gaps = 9/86 (10%) Frame = +2 Query: 36 RVKRNILAMGEKTGMTRNPHFSHDNWIPTFFSTQYFW---FVLKVRWQRLEDTTELG--- 89 R+KR G++ R DN++P F+S +F+ ++ + R+Q E LG Sbjct: 33860 RIKRRQFDGGQQEPQKR------DNFVPQFYSRHWFFLYMYMHRYRYQLDERDLLLGKNV 34021 Query: 90 ---GLAPNCPVVRLSGQRCNIWEFMQ 112 + P CP + G C + E Q Sbjct: 34022 LYHAVHPLCPTLER*GGWCCLVEARQ 34099 >gb|AAQX01000569.1| Phytophthora ramorum strain Pr102 Pramorum_Cont569, whole genome shotgun sequence Length = 122205 Score = 26.6 bits (57), Expect = 8.6 Identities = 9/23 (39%), Positives = 16/23 (69%) Frame = +1 Query: 85 TTELGGLAPNCPVVRLSGQRCNI 107 T+++GG P CP++ L+ + NI Sbjct: 116071 TSDMGGCLPRCPLLALNTPQANI 116139 Database: genome.fa Posted date: Jan 19, 2010 5:07 PM Number of letters in database: 14,903,514 Number of sequences in database: 572 Lambda K H 0.324 0.138 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,805,239 Number of Sequences: 572 Number of extensions: 69243 Number of successful extensions: 390 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 25 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 347 Number of HSP's gapped (non-prelim): 88 length of query: 249 length of database: 4,967,838 effective HSP length: 92 effective length of query: 157 effective length of database: 4,915,214 effective search space: 771688598 effective search space used: 771688598 frameshift window, decay const: 40, 0.1 T: 13 A: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 57 (26.6 bits)