TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000001_1.0 # Protein # Iodothyronine deiodinase 1 (DI1) #
Homo sapiens # Complete
         (249 letters)

Database: genome.fa 
           697 sequences; 71,881,135 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_133                                                           31   1.6  

>scaffold_133
          Length = 262487

 Score = 31.2 bits (69), Expect = 1.6
 Identities = 28/129 (21%), Positives = 55/129 (42%), Gaps = 14/129 (10%)
 Frame = +2

Query: 114   NRPLVLNFGSCTXPSFMFKFDQFKRLIEDFSSIADFLVIYIEEAH---------ASDGWA 164
             NR LV   GS T P +      +      +  I   +++YI+EAH           DGW 
Sbjct: 20654 NRLLVAFAGSIT*PPYREAMADYIAFA*KYKEICKCIIVYIKEAHFVERD*EGKFVDGWP 20833

Query: 165   FK-NNMDIRNHQNLQDRLQAAHLL---LARSPQCPVVVDTMQ-NQSSQLYAALPERLYII 219
                 + +   H++ +DRL+ A  L    +   +  + +D    N   + +   P+ + + 
Sbjct: 20834 IGFYDYEYP*HKSQEDRLKMAKTLKEEYSIPDEFEIYLDIFP*N*FDETFGIWPDNMVVF 21013

Query: 220   QEGRILYKG 228
             +E + +++G
Sbjct: 21014 KELKFIWRG 21040


  Database: genome.fa
    Posted date:  Feb 10, 2010 10:01 AM
  Number of letters in database: 71,881,135
  Number of sequences in database:  697
  
Lambda     K      H
   0.324    0.138    0.448 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,163,368
Number of Sequences: 697
Number of extensions: 265267
Number of successful extensions: 1125
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1080
Number of HSP's gapped (non-prelim): 128
length of query: 249
length of database: 23,960,378
effective HSP length: 103
effective length of query: 146
effective length of database: 23,888,587
effective search space: 3487733702
effective search space used: 3487733702
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 62 (28.5 bits)