TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000015_1.0 # Protein # Selenoprotein I (SelI) # Homo
sapiens # Complete
         (397 letters)

Database: genome.fa 
           23,491 sequences; 86,045,674 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAXJ01000439.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    55   1e-11
gb|AAXJ01000396.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    56   3e-10
gb|AAXJ01000189.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    57   6e-08
gb|AAXJ01013068.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    51   3e-06
gb|AAXJ01009695.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    35   0.26 
gb|AAXJ01000200.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    31   3.1  
gb|AAXJ01001201.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    31   3.3  
gb|AAXJ01000977.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    31   3.3  
gb|AAXJ01000867.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    31   4.0  
gb|AAXJ01000077.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    30   6.3  
gb|AAXJ01000227.1| Perkinsus marinus ATCC 50983 strain ATCC 5098...    30   6.7  

>gb|AAXJ01000439.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296790988,
             whole genome shotgun sequence
          Length = 29359

 Score = 55.1 bits (131), Expect(2) = 1e-11,   Method: Composition-based stats.
 Identities = 24/44 (54%), Positives = 33/44 (75%), Gaps = 1/44 (2%)
 Frame = +1

Query: 97    NFVAY-TLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVT 139
             + V+Y T+D +DGKQARRT S+ PLG+LFDHG DS S  +  ++
Sbjct: 24502 SLVSYQTMDAIDGKQARRTGSAGPLGQLFDHGCDSISTTFICMS 24633



 Score = 34.3 bits (77), Expect(2) = 1e-11,   Method: Composition-based stats.
 Identities = 22/86 (25%), Positives = 34/86 (39%), Gaps = 15/86 (17%)
 Frame = +2

Query: 10    EQLAGFDKYKYSAVDTNPLSLYVMHPFWNTIVKVFPTW---------------LAPNLIT 54
             + L     Y+Y A     L   +   FWN ++   P                 +APN+IT
Sbjct: 24173 QGLKNLSNYEYKAGTPTMLDNLMNKYFWNNVILFMPKKVIGRVLFLLLCPLG*IAPNMIT 24352

Query: 55    FSGFLLVVFNFLLMAYFDPDFYASAP 80
               G   +V ++  +AY+ P F   AP
Sbjct: 24353 LVGLAAMVLSYFCIAYYTPTFTEEAP 24430


>gb|AAXJ01000396.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296192086,
             whole genome shotgun sequence
          Length = 31203

 Score = 55.8 bits (133), Expect(2) = 3e-10,   Method: Composition-based stats.
 Identities = 22/41 (53%), Positives = 31/41 (75%)
 Frame = +2

Query: 102   TLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYS 142
             T+D +DGKQARRT S+ PLG+LFDHG DS S  +  +++ +
Sbjct: 12584 TMDAIDGKQARRTGSAGPLGQLFDHGCDSISTTFICMSIMA 12706



 Score = 28.9 bits (63), Expect(2) = 3e-10,   Method: Composition-based stats.
 Identities = 11/33 (33%), Positives = 21/33 (63%)
 Frame = +3

Query: 48    LAPNLITFSGFLLVVFNFLLMAYFDPDFYASAP 80
             +APN+IT  G + ++ ++  +AY+ P F   +P
Sbjct: 12396 IAPNMITLVGLVAMILSYFSIAYYTPTFTEESP 12494


>gb|AAXJ01000189.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296593782,
             whole genome shotgun sequence
          Length = 49038

 Score = 57.0 bits (136), Expect = 6e-08,   Method: Composition-based stats.
 Identities = 48/150 (32%), Positives = 69/150 (46%), Gaps = 23/150 (15%)
 Frame = +1

Query: 19    KYSAVDTNPLSLYVMHPFWNTIVKV--------------FPTWLAPNLITFSGF----LL 60
             +Y+A +  PL +  M PFWN    V              FP  ++PN IT SG     L+
Sbjct: 29704 RYAAGEYTPLDMVFM-PFWNACTNVIPLPLTNLVDLHQAFPRTISPNAITLSGCAAICLI 29880

Query: 61    VVFNFLLMAYFDPDFYASAPGHKHVPDWVWIVVGILNFVAY-----TLDGVDGKQARRTN 115
              VF  L+       F+ +A        +   V   LN  ++     TLD +DGKQARR  
Sbjct: 29881 YVFIILMPTRHTWHFFMAA-----FCVFAGQVCNHLNADSHLLFPQTLDAMDGKQARRLG 30045

Query: 116   SSTPLGELFDHGLDSWSCVYFVVTVYSIFG 145
              S+PLG + DHG+D+ +    ++TV    G
Sbjct: 30046 VSSPLGAILDHGVDACTMGILMLTVARCLG 30135


>gb|AAXJ01013068.1| Perkinsus marinus ATCC 50983 strain ATCC 50983
           gcontig_1104296727012, whole genome shotgun sequence
          Length = 1299

 Score = 51.2 bits (121), Expect = 3e-06,   Method: Composition-based stats.
 Identities = 38/116 (32%), Positives = 54/116 (46%), Gaps = 12/116 (10%)
 Frame = +3

Query: 42  KVFPTWLAPNLITFSGF----LLVVFNFLLMAYFDPDFYASA--------PGHKHVPDWV 89
           + FP  ++PN IT SG     L+ VF  L+       F+ +A          H H    +
Sbjct: 591 QAFPRTISPNAITLSGCAIICLIYVFIILMPTRHTWHFFMAAFCVFTGQVCNHLHADRHL 770

Query: 90  WIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFG 145
             V         TLD +DGKQARR   S+PLG + DHG+D+ +    ++TV    G
Sbjct: 771 VFV--------QTLDAMDGKQARRLGVSSPLGAILDHGVDACTMGILMLTVARCLG 914


>gb|AAXJ01009695.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296294434,
            whole genome shotgun sequence
          Length = 1621

 Score = 35.0 bits (79), Expect = 0.26,   Method: Composition-based stats.
 Identities = 15/36 (41%), Positives = 22/36 (61%)
 Frame = -2

Query: 110  QARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFG 145
            QARR   S+PLG + DHG+D+ +    ++TV    G
Sbjct: 1620 QARRLGVSSPLGAILDHGVDACTMGILMLTVARCLG 1513


>gb|AAXJ01000200.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296618966,
             whole genome shotgun sequence
          Length = 47923

 Score = 31.2 bits (69), Expect = 3.1,   Method: Composition-based stats.
 Identities = 12/29 (41%), Positives = 18/29 (62%)
 Frame = -3

Query: 298   TAFANSTCQLIVCQMSSTRCPTLNWLLVP 326
             TA++N  C+L    M+  R PT+ W L+P
Sbjct: 44915 TAYSNRGCRL*AADMNKRRRPTVEWKLLP 44829


>gb|AAXJ01001201.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296703466,
            whole genome shotgun sequence
          Length = 13368

 Score = 31.2 bits (69), Expect = 3.3,   Method: Composition-based stats.
 Identities = 15/46 (32%), Positives = 20/46 (43%)
 Frame = +3

Query: 86   PDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSW 131
            P WVW + G++       D +DGKQ    +S   L   F  G   W
Sbjct: 3603 PGWVWALTGVMVLAYQLFDNLDGKQVEAKDSFPALKP*FISGPSPW 3740


>gb|AAXJ01000977.1| Perkinsus marinus ATCC 50983 strain ATCC 50983
           gcontig_1104296770586, whole genome shotgun sequence
          Length = 16228

 Score = 31.2 bits (69), Expect = 3.3,   Method: Composition-based stats.
 Identities = 15/46 (32%), Positives = 20/46 (43%)
 Frame = +3

Query: 86  PDWVWIVVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSW 131
           P WVW + G++       D +DGKQ    +S   L   F  G   W
Sbjct: 3   PGWVWALTGVMVLAYQLFDNLDGKQVEAKDSFPALKP*FISGPSPW 140


>gb|AAXJ01000867.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296286832,
            whole genome shotgun sequence
          Length = 17753

 Score = 30.8 bits (68), Expect = 4.0,   Method: Composition-based stats.
 Identities = 17/56 (30%), Positives = 26/56 (46%), Gaps = 1/56 (1%)
 Frame = +2

Query: 276  WILWSPSDILELHPRVFYFMVGTAFANSTCQLIVCQMSSTRC-PTLNWLLVPLFLV 330
            W LWSP D  +  P +F     T   +  C L + Q+ S +  P   W  VP+ ++
Sbjct: 7478 WTLWSPGD--DSSPGLFALFPFTRNTSVGCTLRMQQLQSCKVRPQFFWEFVPMLVI 7639


>gb|AAXJ01000077.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296710794,
             whole genome shotgun sequence
          Length = 71857

 Score = 30.4 bits (67), Expect = 6.3,   Method: Composition-based stats.
 Identities = 23/71 (32%), Positives = 37/71 (52%)
 Frame = +1

Query: 92    VVGILNFVAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWSCVYFVVTVYSIFGRGSTGV 151
             V G LNF      G DG+Q      S  L  +F H    WSC+Y +VT+  +    ++G+
Sbjct: 48004 VFGRLNFPESPYIGYDGRQ----R*SHVLIVIFSHTECRWSCLYSLVTLAILSISTASGL 48171

Query: 152   SVFVLYLLLWV 162
             S+ ++ + LW+
Sbjct: 48172 SLQIV*IPLWI 48204


>gb|AAXJ01000227.1| Perkinsus marinus ATCC 50983 strain ATCC 50983 gcontig_1104296140586,
             whole genome shotgun sequence
          Length = 42706

 Score = 30.0 bits (66), Expect = 6.7,   Method: Composition-based stats.
 Identities = 23/55 (41%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
 Frame = -1

Query: 216   FLYRDLFTAMI---IGCALCVTLPMSLLNFFRSYKNNTLKLNSVYEAMVPLFSPC 267
             FLYR   + MI   I  A C T   SLL  +R+Y   TL   S+   +VPL  PC
Sbjct: 28279 FLYRG*NSMMISNFITIAECTTKERSLLMRYRTYSMVTL---SMVTRLVPLTEPC 28124


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 86,045,674
  Number of sequences in database:  23,491
  
Lambda     K      H
   0.329    0.142    0.464 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 23491
Number of Hits to DB: 46,139,236
Number of extensions: 766085
Number of successful extensions: 5186
Number of sequences better than 10.0: 18
Number of HSP's gapped: 5179
Number of HSP's successfully gapped: 20
Length of query: 397
Length of database: 28,681,891
Length adjustment: 109
Effective length of query: 288
Effective length of database: 26,121,372
Effective search space: 7522955136
Effective search space used: 7522955136
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 42 (20.8 bits)
