TBLASTN 2.2.19 [Nov-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= DDB0304589 |DDB_G0277015 |Protein| gene: msp on chromosome: 2
position 7225040 to 7225867
         (243 letters)

Database: genome.fa 
           799 sequences; 32,967,507 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_17                                                           297   5e-81
scaffold_168                                                           28   4.9  

>scaffold_17 
          Length = 153674

 Score =  297 bits (760), Expect = 5e-81,   Method: Compositional matrix adjust.
 Identities = 154/266 (57%), Positives = 178/266 (66%), Gaps = 24/266 (9%)
 Frame = -1

Query: 1     MSLF-NLPKVDLGEDCEGGVUARPXXXXXXINN-ASDESTPLISKTNDEEK-ANIGISST 57
             MS F NLPKV++G+DCEGGV ARP      IN    DESTPLISKT+DEEK      S +
Sbjct: 39995 MSFFKNLPKVEVGDDCEGGV*ARPTSSASSINKTVGDESTPLISKTSDEEKDIACSSSPS 39816

Query: 58    SNSPQEEQTKKPXXXXXXXXXXXXPALVGSUCWPXXXXXXXXXXXXXXXXXXXHSLTFAI 117
             S+ PQEEQ+KKP            PALVGS CWP                   H++T+AI
Sbjct: 39815 SDQPQEEQSKKPLIISILTVLISIPALVGS*CWPILLSSILGIAVSGASVTFAHAITYAI 39636

Query: 118   TLSILSNLAQYHFHKCKKRPSDRGHWIKFGPFYLTAIAVPLATFDILRHILVDNSIW--- 174
             TL+ILSNLAQYH+HKC+ RP ++GHW K+GPFYLT +AVPLATFDILRHILVDN +W   
Sbjct: 39635 TLAILSNLAQYHYHKCQSRPREKGHWHKYGPFYLTLLAVPLATFDILRHILVDNQLWVNR 39456

Query: 175   ------------------TIHSFISPAAYRPGCENENITCLSVMGWFSAIVFTYTGYACL 216
                               T+HSF+SPAAYRPGC +ENI CLSVMGWFSAI+FTYTGYA L
Sbjct: 39455 HKFKK*KKNFLLIFLCY*TVHSFVSPAAYRPGCTHENIRCLSVMGWFSAIIFTYTGYALL 39276

Query: 217   LVGTIWAADLIPKIKKVWTQLRPSKK 242
             L GTIW A+L  KIKK+W QLRP KK
Sbjct: 39275 LTGTIWCAELHIKIKKLWQQLRPKKK 39198


>scaffold_168 
          Length = 64553

 Score = 28.5 bits (62), Expect = 4.9,   Method: Compositional matrix adjust.
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -1

Query: 190   ENENITCLSVMGWFSAIVFTYTGYACLLVG 219
             E +    L+ +GW S +  TY+ Y CLLVG
Sbjct: 26417 EGDTYFSLASLGWGSGV--TYSTYGCLLVG 26334



 Score = 28.5 bits (62), Expect = 5.3,   Method: Compositional matrix adjust.
 Identities = 12/23 (52%), Positives = 16/23 (69%)
 Frame = -2

Query: 197   LSVMGWFSAIVFTYTGYACLLVG 219
             L+ +GW S +  TY+ Y CLLVG
Sbjct: 29128 LASLGWESGV--TYSAYGCLLVG 29066


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:50 PM
  Number of letters in database: 32,967,507
  Number of sequences in database:  799
  
Lambda     K      H
   0.321    0.135    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 799
Number of Hits to DB: 8,224,664
Number of extensions: 118432
Number of successful extensions: 491
Number of sequences better than 10.0: 3
Number of HSP's gapped: 487
Number of HSP's successfully gapped: 4
Length of query: 243
Length of database: 10,989,169
Length adjustment: 98
Effective length of query: 145
Effective length of database: 10,910,867
Effective search space: 1582075715
Effective search space used: 1582075715
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 49 (23.5 bits)