TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= ENST00000254730 peptide:ENSP00000254730
pep:KNOWN_protein_coding
         (596 letters)

Database: genome.fa 
           5966 sequences; 17,086,925 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAAI01002332.1| Plasmodium berghei whole genome shotgun asse...   149   4e-36
emb|CAAI01002424.1| Plasmodium berghei whole genome shotgun asse...    72   7e-16
emb|CAAI01007252.1| Plasmodium berghei whole genome shotgun asse...    70   2e-12
emb|CAAI01004904.1| UNBERG.2.19835, whole genome shotgun sequence      70   2e-12
emb|CAAI01002308.1| Plasmodium berghei whole genome shotgun asse...    45   9e-05
emb|CAAI01001768.1| Plasmodium berghei whole genome shotgun asse...    37   0.030
emb|CAAI01002147.1| Plasmodium berghei whole genome shotgun asse...    35   0.060
emb|CAAI01006812.1| Plasmodium berghei whole genome shotgun asse...    35   0.091
emb|CAAI01002935.1| Plasmodium berghei whole genome shotgun asse...    34   0.15 
emb|CAAI01000499.1| Plasmodium berghei whole genome shotgun asse...    33   0.25 
emb|CAAI01002451.1| Plasmodium berghei whole genome shotgun asse...    33   0.28 
emb|CAAI01000592.1| Plasmodium berghei whole genome shotgun asse...    33   0.41 
emb|CAAI01000782.1| Plasmodium berghei whole genome shotgun asse...    28   9.1  

>emb|CAAI01002332.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP2667,
            whole genome shotgun sequence
          Length = 11746

 Score =  149 bits (375), Expect = 4e-36,   Method: Composition-based stats.
 Identities = 128/511 (25%), Positives = 223/511 (43%), Gaps = 87/511 (17%)
 Frame = -2

Query: 87   QVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLN 146
            Q+ LVDCPGH SLI+ I+ GA+I+D++ LVID+ KG+Q Q+ ECLV+ Q     ++++LN
Sbjct: 6597 QICLVDCPGHHSLIKCIVMGAKIMDMIFLVIDINKGIQKQTIECLVLCQFVKSDIIIILN 6418

Query: 147  KIDLLPEGKRQAAIDKMTKKMQKTLENTKFR--------------------------GAP 180
            KIDL+P  +R   I+ M KK++  +  T  +                          G  
Sbjct: 6417 KIDLIPLHERDKKINSMKKKIKDAINRTSLKKLSYYIVSLSANRKNRDDNEIDDVEIGKK 6238

Query: 181  IIPVXXXXXXX-------XXXXXXXXXXIPELIELLTSQISIPTRDPSGP----FLMSVD 229
            II +                        I EL+ELL S I IP R+ + P    F    D
Sbjct: 6237 IISMCRNSPNELNPHFNILKKEKNTRENINELVELLKSVIKIPVRE-TNPTFEDFYFLYD 6061

Query: 230  HCFSIKGQGTVMTGTILSGSISLGDSVEIPALKVVKKVKSMQMFHMPITSAMQGDRLGIC 289
            H F IKG+G V TGT++ G I++   + I  L +  K+K +Q F         G+RL   
Sbjct: 6060 HSFEIKGKGMVYTGTVIKGKITINSDISILPLNIRGKIKDIQSFKKKQKEGTSGNRLSFL 5881

Query: 290  VTQFDPKLL---ERGLVCAPESLHTVHAALI-SVEKIPYFRGPLQTKAKFHITVGHETVM 345
            ++  + K L   ERG++   +S     A  I  ++ I +++  ++ K      +G  +  
Sbjct: 5880 ISHDNIKNLKKVERGIIVHEKSNICNFAVFICKIKLIEFYKKSIKNKELLTCIIGFASSH 5701

Query: 346  GRLMFFSPAPDNFDQEPILD------------------------SFNFSQEYLF--QEQY 379
                FF     +  +E  ++                         F+ +  YL   + +Y
Sbjct: 5700 SYGFFFKKLKTDPKKEQTINVQNNEHNNGQRGGETYSTCLSNNIGFDRNGNYLIIDEVEY 5521

Query: 380  LSKDLTPAVTDND--EADKKAGQATEGHCPRQQWALVEFEKPVTCPRLCLVIGSRLDADI 437
            ++      V++ND  E++K+  Q  E +   + + LV  +K +        I  + D +I
Sbjct: 5520 INHIDDGTVSENDLFESEKEY-QNNERN--EEIYFLVILKKKMYGFNGEKCIFLKNDENI 5350

Query: 438  HTNTCRLAFHGILLHGLEDRNYADSF------------------LPRLKVYKLKHKHGLV 479
            +   CR+  HG ++  ++D                            LK+ K K K GL+
Sbjct: 5349 N---CRICLHGNIVDIIKDSAVNSKINNSTFVMNKKPCLNDHEDYKNLKIMKEKEKIGLI 5179

Query: 480  ERAMDDYSVIGRSLFKKETNIQLFVGLKVHL 510
            E+ +D+ +++ +++F     +  ++  K++L
Sbjct: 5178 EKVIDNTTLLCKNVFASSNQVTAYLTQKIYL 5086



 Score = 45.8 bits (107), Expect = 4e-05,   Method: Composition-based stats.
 Identities = 21/46 (45%), Positives = 26/46 (56%)
 Frame = -2

Query: 13   LGHIDSGKXXXXXXXXXXXXXXXFDKQPQSRERGITLDLGFSCFSV 58
            LGH+DSGK                DK  QS+E+GIT+DLGFS F +
Sbjct: 6960 LGHVDSGKTSLCRCLSEVLSTCALDKHRQSQEKGITIDLGFSSFYI 6823


>emb|CAAI01002424.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP2768,
            whole genome shotgun sequence
          Length = 12274

 Score = 72.0 bits (175), Expect(2) = 7e-16,   Method: Composition-based stats.
 Identities = 61/247 (24%), Positives = 99/247 (40%), Gaps = 14/247 (5%)
 Frame = +3

Query: 13   LGHIDSGKXXXXXXXXXX---------XXXXXFDKQPQSRERGITLDLGFSCFSVPLPAR 63
            +GH+D GK                         DK P+ ++RGIT++             
Sbjct: 8763 IGHVDHGKTTLTAAITKVCSKYDRGTFKSYEDIDKTPEEQKRGITIN------------- 8903

Query: 64   LRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGHASL-IRTIIGGAQIIDLMMLVIDVTKG 122
                                     + +DCPGH    I+ +I G   +D  +LV+    G
Sbjct: 8904 ------------ATHVEYETEKRHYSHIDCPGHLDYYIKNMITGTSQMDGSILVVSAYDG 9047

Query: 123  MQTQSAECLVIG-QIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPI 181
            +  Q+ E +++  QI   K++V LNKID+  +   Q  +D +  ++++ L   K+ G  I
Sbjct: 9048 LMPQTKEHVLLSRQIGINKIIVYLNKIDMCDD---QELVDLVELEVRELLSFHKYDGDNI 9218

Query: 182  IPVXXXXXXXXXXXXXXXXXIPELIELLTS---QISIPTRDPSGPFLMSVDHCFSIKGQG 238
             P                  +P +++LL +    I  P R    PFLMS+D    I G+G
Sbjct: 9219 -PFIKGSALKALNDDPSEYGVPSILKLLDACDNYIDEPKRKIDLPFLMSIDDVLQISGKG 9395

Query: 239  TVMTGTI 245
            TV TG +
Sbjct: 9396 TVATGRV 9416



 Score = 30.0 bits (66), Expect(2) = 7e-16,   Method: Composition-based stats.
 Identities = 15/59 (25%), Positives = 31/59 (52%), Gaps = 3/59 (5%)
 Frame = +2

Query: 248  GSISLGDSVEIPALK---VVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLV 303
            G+I + + V+I  +K   +   +  ++MF   + +A  GD++GI +       + RG+V
Sbjct: 9422 GTIKINEPVDIIGIKEKSIKTVITGIEMFRKTLDTAQAGDQIGIMLKNVKKNDISRGMV 9598


>emb|CAAI01007252.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP0669,
            whole genome shotgun sequence
          Length = 15829

 Score = 70.5 bits (171), Expect = 2e-12,   Method: Composition-based stats.
 Identities = 67/234 (28%), Positives = 110/234 (47%), Gaps = 23/234 (9%)
 Frame = -1

Query: 87   QVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL 145
             V+ VDCPGH  L+ T++ GA ++D  +L++   +   Q Q++E L   +I   K +++L
Sbjct: 4174 HVSFVDCPGHDILMATMLNGAAVMDAALLLVAGNESCPQPQTSEHLAAVEIMRLKHILIL 3995

Query: 146  -NKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXXXXIPE 204
             NK++L+ E   QA   K  ++++  +  T    APIIP+                 I  
Sbjct: 3994 QNKVELIKE--EQAL--KQQEEIRNFVSGTAADSAPIIPI----------SAVLKYNIDV 3857

Query: 205  LIELLTSQISIPTRDPSGPFLMSVDHCFSIKG--------QGTVMTGTILSGSISLGDSV 256
            + E + +QISIP RD      M V   F +          QG V  G+IL G + +GD +
Sbjct: 3856 VCEYIVTQISIPRRDFISSPHMIVIRSFDVNKPGEDIETLQGGVAGGSILHGVLKVGDQI 3677

Query: 257  EI-PAL------------KVVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKL 297
            EI P +             ++ K+ SM   +  +  A+ G  +G+  T+ DP L
Sbjct: 3676 EIRPGIISKDDKGEITCRPIISKILSMFAENNNLKYAVPGGLIGVG-TRIDPIL 3518


>emb|CAAI01004904.1| UNBERG.2.19835, whole genome shotgun sequence
          Length = 1711

 Score = 70.5 bits (171), Expect = 2e-12,   Method: Composition-based stats.
 Identities = 48/170 (28%), Positives = 83/170 (48%), Gaps = 6/170 (3%)
 Frame = -2

Query: 140  KLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPIIPVXXXXXXXXXXXXXXX 199
            K++V LNKID+  +   Q  +D +  ++++ L   K+ G  I P                
Sbjct: 1710 KIIVYLNKIDMCDD---QELVDLVELEVRELLSFHKYDGDNI-PFIKGSALKALNDDPSE 1543

Query: 200  XXIPELIELLTS---QISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSV 256
              +P +++LL +    I  P R    PFLMS+D    I G+GTV TG +  G+I + + V
Sbjct: 1542 YGVPSILKLLDACDNYIDEPKRKIDLPFLMSIDDVLQISGKGTVATGRVEQGTIKINEPV 1363

Query: 257  EIPALK---VVKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLV 303
            +I  +K   +   +  ++MF   + +A  GD++GI +       + RG+V
Sbjct: 1362 DIIGIKEKSIKTVITGIEMFRKTLDTAQAGDQIGIMLKNVKKNDISRGMV 1213


>emb|CAAI01002308.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP2639,
            whole genome shotgun sequence
          Length = 7624

 Score = 44.7 bits (104), Expect = 9e-05,   Method: Composition-based stats.
 Identities = 23/65 (35%), Positives = 38/65 (58%), Gaps = 1/65 (1%)
 Frame = +2

Query: 88   VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECL-VIGQIACQKLVVVLN 146
            + ++D PGH S       G+ + D+ +LVID+  G++ Q+ E + ++ Q  C   V+ LN
Sbjct: 5027 IMIIDTPGHESFYNLRKRGSSLCDIAILVIDLMHGLEQQTKESIQILKQRNC-PFVIALN 5203

Query: 147  KIDLL 151
            KID L
Sbjct: 5204 KIDRL 5218


>emb|CAAI01001768.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP2022,
           whole genome shotgun sequence
          Length = 1194

 Score = 36.6 bits (83), Expect = 0.030,   Method: Composition-based stats.
 Identities = 23/87 (26%), Positives = 32/87 (36%)
 Frame = +1

Query: 41  QSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPGHASLI 100
           Q RERGIT+   +SCF                               V L+D PGH    
Sbjct: 301 QERERGITIKTAYSCFK-------------------------WNNVNVNLIDTPGHIDFS 405

Query: 101 RTIIGGAQIIDLMMLVIDVTKGMQTQS 127
                   + D  ++VID  +G+Q Q+
Sbjct: 406 NETFLSLCVSDKCVIVIDAKEGLQIQT 486


>emb|CAAI01002147.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP2463,
            whole genome shotgun sequence
          Length = 8026

 Score = 35.4 bits (80), Expect = 0.060,   Method: Composition-based stats.
 Identities = 19/61 (31%), Positives = 32/61 (52%)
 Frame = +1

Query: 90   LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149
            L+D PGH      +     + +  +L+ID  KG+Q Q+    +  +    K++ V+NKID
Sbjct: 2863 LIDTPGHFDFYHEVKRSLNVCEGAILLIDGGKGIQAQTLNIFLEIKKHNIKIIPVINKID 3042

Query: 150  L 150
            L
Sbjct: 3043 L 3045


>emb|CAAI01006812.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP2923,
           whole genome shotgun sequence
          Length = 1466

 Score = 35.0 bits (79), Expect = 0.091,   Method: Composition-based stats.
 Identities = 19/70 (27%), Positives = 34/70 (48%), Gaps = 5/70 (7%)
 Frame = -3

Query: 87  QVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVT-----KGMQTQSAECLVIGQIACQKL 141
           +V + D PGH  L+  +   +   D  +LV+D       K  +T    C ++  +    +
Sbjct: 429 KVNIFDTPGHNELVNNLHACSFFADCAILVVDANNIYNKKNDETYRNVC-ILKSVGISNI 253

Query: 142 VVVLNKIDLL 151
           ++V+NKIDL 
Sbjct: 252 IIVINKIDLF 223


>emb|CAAI01002935.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP3350,
            whole genome shotgun sequence
          Length = 6208

 Score = 34.3 bits (77), Expect = 0.15,   Method: Composition-based stats.
 Identities = 23/87 (26%), Positives = 31/87 (35%)
 Frame = +3

Query: 36   FDKQPQSRERGITLDLGFSCFSVPLPARLRSSXXXXXXXXXXXXXXXXXXXQVTLVDCPG 95
             DK    RERGIT+D+    F  P                             T +D PG
Sbjct: 1416 LDKLKAERERGITIDIALWKFETP-------------------------RYFFTDIDAPG 1520

Query: 96   HASLIRTIIGGAQIIDLMMLVIDVTKG 122
            H   I+ +I G    D+ +LV+    G
Sbjct: 1521 HKDFIKNMITGTYQADVALLVVPAEVG 1601


>emb|CAAI01000499.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP3952,
            whole genome shotgun sequence
          Length = 3095

 Score = 33.5 bits (75), Expect = 0.25,   Method: Composition-based stats.
 Identities = 14/36 (38%), Positives = 22/36 (61%)
 Frame = -3

Query: 87   QVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKG 122
            + T++D PGH + I  +I GA   D+ +L+I   KG
Sbjct: 1077 RFTILDAPGHKNFIPNMISGAAQADIGVLIISARKG 970


>emb|CAAI01002451.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP2798,
           whole genome shotgun sequence
          Length = 4877

 Score = 33.1 bits (74), Expect = 0.28,   Method: Composition-based stats.
 Identities = 20/73 (27%), Positives = 39/73 (53%), Gaps = 1/73 (1%)
 Frame = -2

Query: 90  LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQ-KLVVVLNKI 148
           +VD PGH+     +     +ID + L++DV +G + Q+   L    +  + K++V++NK 
Sbjct: 982 IVDTPGHSDFGGEVERVLNLIDGVCLIVDVVEGPKNQTKFVLKKSLLNPKCKIIVIMNKF 803

Query: 149 DLLPEGKRQAAID 161
           D     K++  I+
Sbjct: 802 DKPSNIKKKEEIE 764


>emb|CAAI01000592.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP0676,
            whole genome shotgun sequence
          Length = 6537

 Score = 32.7 bits (73), Expect = 0.41,   Method: Composition-based stats.
 Identities = 21/88 (23%), Positives = 43/88 (48%), Gaps = 2/88 (2%)
 Frame = +2

Query: 88   VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNK 147
            + L+D PGH      +    ++ D  ++V+D  +G+  Q+   L        K V+ +NK
Sbjct: 1208 INLIDSPGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLYQALGERIKPVLHVNK 1387

Query: 148  ID--LLPEGKRQAAIDKMTKKMQKTLEN 173
            +D  LL   + Q  ++ + +   +T+E+
Sbjct: 1388 VDRALL---ELQMEVEDIYQTFARTIES 1462


>emb|CAAI01000782.1| Plasmodium berghei whole genome shotgun assembly, contig PB_RP0886,
            whole genome shotgun sequence
          Length = 6598

 Score = 28.1 bits (61), Expect = 9.1,   Method: Composition-based stats.
 Identities = 24/97 (24%), Positives = 47/97 (48%), Gaps = 3/97 (3%)
 Frame = -3

Query: 88   VTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKL--VVVL 145
            + ++D PGH      +    +++D  +LVI    G+Q+Q+    V  Q+    +  ++ +
Sbjct: 3164 INIIDTPGHVDFTIEVERSLRVLDAAVLVICGVSGVQSQTLT--VNRQMDRYHIPRILFI 2991

Query: 146  NKIDLLPEGKRQAAIDKMTKKMQKTLE-NTKFRGAPI 181
            NK+D     +  A I++  + ++K L  NT     PI
Sbjct: 2990 NKLD-----RDGANIERTLETIEKKLNLNTILLQIPI 2895


  Database: genome.fa
    Posted date:  Jan 16, 2009 12:51 PM
  Number of letters in database: 17,086,925
  Number of sequences in database:  5966
  
Lambda     K      H
   0.321    0.137    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5966
Number of Hits to DB: 8,617,787
Number of extensions: 107756
Number of successful extensions: 286
Number of sequences better than 10.0: 14
Number of HSP's gapped: 284
Number of HSP's successfully gapped: 19
Length of query: 596
Length of database: 5,695,641
Length adjustment: 101
Effective length of query: 495
Effective length of database: 5,093,075
Effective search space: 2521072125
Effective search space used: 2521072125
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 43 (21.2 bits)
