TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000004_1.0 # Protein # Glutathione peroxidase 1 (GPx1) #
Homo sapiens # Complete
         (203 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            131   5e-31
scaffold_34                                                            72   5e-13
scaffold_69                                                            68   7e-12
scaffold_8                                                             67   2e-11
scaffold_972                                                           44   2e-04
scaffold_16                                                            29   4.4  
scaffold_58                                                            28   5.8  
scaffold_20                                                            28   5.8  
scaffold_3                                                             28   5.8  
scaffold_41                                                            28   7.5  
scaffold_4                                                             28   9.8  

>scaffold_6
          Length = 2755913

 Score =  131 bits (330), Expect = 5e-31
 Identities = 74/142 (52%), Positives = 90/142 (63%), Gaps = 6/142 (4%)
 Frame = +2

Query: 37      GKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSL 96
               G+V+L+ENVAS+ GTTVRD+TQMN+L  +   +    LGFP NQFG Q N K+ E+L  L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 97      KYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTD----PKLITW 152
                +VRPGGGF PNF +F K E NG GA  LF FLR +L APSDD     +D     K I W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 153     SPVCRND--VAWNFEKFLVGPD 172
               +PV R D      FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 83.6 bits (205), Expect = 2e-16
 Identities = 69/179 (38%), Positives = 84/179 (46%)
 Frame = +3

Query: 19      FSARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPC 78
               F+  PL GG  V+L +   +  LI N AS  G T +       LQ + G R L VL FPC
Sbjct: 2424462 FAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR-LQVLAFPC 2424635

Query: 79      NQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSD 138
               +QF  QE     EI            F   F LF K +VNG  AHPLFAFL+EA    + 
Sbjct: 2424636 DQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAA 2424797

Query: 139     DATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLS 197
                        +  T       DV WNF K+LV  DG P +RYS   +   IE DI A L+
Sbjct: 2424798 PVPPWNNKGQAAT-------DVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAALN 2424950



 Score = 80.9 bits (198), Expect = 1e-15
 Identities = 63/169 (37%), Positives = 88/169 (52%), Gaps = 2/169 (1%)
 Frame = +2

Query: 14      QSVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPR--GL 71
               ++ + F   PL GG P++    R +  L+ N AS  G       Q+ ELQ  L  R  GL
Sbjct: 1870685 RTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFA----PQLRELQA-LWARYPGL 1870849

Query: 72      VVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLRE 131
                VL FPC+QFG QE     +I ++  Y R G     +F +FEK +VNG  AHPLF +L++
Sbjct: 1870850 AVLAFPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNGPRAHPLFTYLKK 1871011

Query: 132     ALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYS 180
               A            T  + +    + R++V WNF K+L+  DG  LRRYS
Sbjct: 1871012 A------------TAGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYS 1871119



 Score = 59.7 bits (143), Expect = 2e-09
 Identities = 31/67 (46%), Positives = 40/67 (59%)
 Frame = +1

Query: 134     PAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIE 193
               PA  DD   + T  K  + S     D+AWNFEKFL+  +G P++RYS  F T D+  D+E
Sbjct: 1233802 PASCDDFREIWTG-KRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVE 1233978

Query: 194     ALLSQGP 200
               ALL  GP
Sbjct: 1233979 ALLEHGP 1233999



 Score = 28.5 bits (62), Expect = 5.8
 Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
 Frame = +3

Query: 22     RPLAGGEPVSLGSLR--GKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLG 75
              RP+  G       LR  G+  L ++   L G  +R   +M  L RRLGPRG +  G
Sbjct: 385173 RPVRDGPLALEEELRVVGEERLEQHAERLRGRRIRAGPRMASLGRRLGPRGRLSAG 385340


>scaffold_34
          Length = 430012

 Score = 72.0 bits (175), Expect = 5e-13
 Identities = 55/180 (30%), Positives = 78/180 (43%), Gaps = 10/180 (5%)
 Frame = -1

Query: 27     GEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQEN 86
              G  V   + RG+V+ + N+AS  G T   Y  + +L  +   RGL ++GFPCNQF  QE 
Sbjct: 212191 GAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFPCNQFMGQEP 212012

Query: 87     AKNEEILNSLKYVRPGGGFEP---------NFMLFEKCEVNGAGAHPLFAFLREALPAPS 137
                  EI     + R  G  +P          F L  KC+V+GA   P+F++L+   P   
Sbjct: 212011 GAESEI---KAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKREAP--- 211850

Query: 138    DDATALMTDPKLITWSPVCRNDVAWNF-EKFLVGPDGVPLRRYSRRFQTIDIEPDIEALL 196
                                C+  ++WNF   FLV  DG             D+ P +EALL
Sbjct: 211849 ------------------CK--ISWNFGAYFLVSRDGAV--EAHENAHPRDLAPRVEALL 211736


>scaffold_69
          Length = 78151

 Score = 68.2 bits (165), Expect = 7e-12
 Identities = 41/103 (39%), Positives = 53/103 (51%)
 Frame = +2

Query: 28    EPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENA 87
             EP+ LG        I +  S  G T   Y+QM EL   LG  GL +L FPCNQFG+QE A
Sbjct: 68645 EPLKLG--------ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPA 68800

Query: 88    KNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLR 130
               E +    K       +  +F + EK  V+G  AHP++ FLR
Sbjct: 68801 TCEVVAEFAK-----SAYGADFTMMEKVAVHGPRAHPVYNFLR 68914



 Score = 28.9 bits (63), Expect = 4.4
 Identities = 19/56 (33%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
 Frame = +3

Query: 15    SVYAFSARPLAGGEPVSLGSLRGKVLLIENVASLXG-TTVRDYTQMNELQRRLGPR 69
             S Y   A+  AG   ++  + +GKV+L+ NVAS  G T   + +    LQR    R
Sbjct: 68385 SFYDLKAKTAAGAT-LTFDAFKGKVVLLTNVASA*GKTAAHEKSDSTSLQRECSAR 68549


>scaffold_8
          Length = 1961717

 Score = 66.6 bits (161), Expect = 2e-11
 Identities = 47/157 (29%), Positives = 72/157 (45%)
 Frame = +3

Query: 27      GEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQEN 86
               G+P  L   RG   L+ N+         ++  +  +      +GL V  FP  Q  ++ +
Sbjct: 1062486 GKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQGYYEPD 1062665

Query: 87      AKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTD 146
                   E++ +  Y + G G  P  ++F+K +V G  AHPL+ +L            AL T+
Sbjct: 1062666 VS--ELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL------------ALATN 1062803

Query: 147     PKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRF 183
               P  +    V       NFEKFL+  DG PLRRY R+F
Sbjct: 1062804 PNGVGRLTV-------NFEKFLLDGDGKPLRRYPRKF 1062893


>scaffold_972
          Length = 3742

 Score = 43.5 bits (101), Expect = 2e-04
 Identities = 42/148 (28%), Positives = 54/148 (36%), Gaps = 1/148 (0%)
 Frame = +3

Query: 27   GEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQEN 86
            G  V+     GKV    NVAS  G T   Y  M +L    G   L V+ FP  +FG QE 
Sbjct: 3279 GSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIAFPTGEFGGQE- 3452

Query: 87   AKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTD 146
               +E +    Y R      P  +L +K ++  A    + A      P            
Sbjct: 3453 LPTDEAIAKFAYDRVKFPAAPRGVLLQKADLGRASWQAMQAEAGADAP------------ 3596

Query: 147  PKLITWSPVCRNDVAWNFE-KFLVGPDG 173
                          AWNF+ KFLV  DG
Sbjct: 3597 --------------AWNFKGKFLVSKDG 3638


>scaffold_16
          Length = 966663

 Score = 28.9 bits (63), Expect = 4.4
 Identities = 24/87 (27%), Positives = 39/87 (44%), Gaps = 3/87 (3%)
 Frame = +2

Query: 118    NGAGAHPLFAFLREALPAPSDDATALMTDP--KLITWSPVCRNDVAWNFEKFLVGPDGVP 175
              + A +HP  A L  A+ A +  A  + T P  +L +W P+     +W    ++ G + +P
Sbjct: 803633 HSAPSHPSGAALGAAVSAAA--APTVFTTPALQLTSWRPMASKVTSW--RSWIFGTEKMP 803800

Query: 176    LRRYSR-RFQTIDIEPDIEALLSQGPS 201
               +   R    T D EP +    S  PS
Sbjct: 803801 SQSQFRIAVSTTDAEPRVTTTPSSQPS 803881


>scaffold_58
          Length = 213184

 Score = 28.5 bits (62), Expect = 5.8
 Identities = 16/43 (37%), Positives = 19/43 (44%), Gaps = 4/43 (9%)
 Frame = +2

Query: 100    RPGGG----FEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSD 138
              R GGG     EP  +L E C   G G HP  +  R+  P   D
Sbjct: 170612 RQGGGRAGAVEPRLVLRESCRHRGGGRHPAASRCRDWQPRRRD 170740


>scaffold_20
          Length = 721792

 Score = 28.5 bits (62), Expect = 5.8
 Identities = 18/48 (37%), Positives = 19/48 (39%)
 Frame = +1

Query: 100    RPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDP 147
              RPG G EP      +    GAGA P     R A P P   A    T P
Sbjct: 298288 RPGAGAEPRLRR-PRARARGAGARPRPPAPRPAAPPPPRGAPRAATPP 298428


>scaffold_3
          Length = 3001400

 Score = 28.5 bits (62), Expect = 5.8
 Identities = 17/62 (27%), Positives = 26/62 (41%)
 Frame = +2

Query: 44      NVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGG 103
               NV +     V D T    L+  + PRG V+ G   + F  +      E+ N+     PG 
Sbjct: 1322372 NVDATYLLPVDDATVREALEETMAPRGAVIRGAGSSGFLRRSKVSETELRNAADRPVPGA 1322551

Query: 104     GF 105
               G+
Sbjct: 1322552 GW 1322557


>scaffold_41
          Length = 407180

 Score = 28.1 bits (61), Expect = 7.5
 Identities = 14/41 (34%), Positives = 21/41 (51%)
 Frame = +2

Query: 122    AHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAW 162
              A+ L +  R  + A SD   A     +L+ W+PV + D AW
Sbjct: 228698 ANTLISLARAPVAAASDGLAA-----RLLRWAPVVQGDAAW 228805


>scaffold_4
          Length = 3024044

 Score = 27.7 bits (60), Expect = 9.8
 Identities = 17/44 (38%), Positives = 24/44 (54%)
 Frame = -2

Query: 111     LFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSP 154
               LFE+ E +G  A  L   LR++LPA +  A A    P +I+  P
Sbjct: 1000981 LFERQEDSGFCAELLVLDLRKSLPATASPAPAAPPAPAVISSEP 1000850


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.321    0.139    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,682,256
Number of Sequences: 1185
Number of extensions: 301673
Number of successful extensions: 1284
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 648
Number of HSP's gapped (non-prelim): 938
length of query: 203
length of database: 18,886,859
effective HSP length: 99
effective length of query: 104
effective length of database: 18,769,544
effective search space: 1952032576
effective search space used: 1952032576
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000005_1.0 # Protein # Glutathione peroxidase 2 (GPx2) #
Homo sapiens # Complete
         (190 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            116   2e-26
scaffold_34                                                            78   7e-15
scaffold_8                                                             60   1e-09
scaffold_972                                                           56   3e-08
scaffold_69                                                            51   7e-07
scaffold_23                                                            34   0.093
scaffold_2                                                             33   0.21 
scaffold_19                                                            28   6.6  

>scaffold_6
          Length = 2755913

 Score =  116 bits (290), Expect = 2e-26
 Identities = 66/153 (43%), Positives = 88/153 (57%), Gaps = 6/153 (3%)
 Frame = +2

Query: 28      GRAVLIENVASLXGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLK 87
               G+ VL+ENVAS+ GTT RDFTQ+N+L  +F  +   LGFP NQFG Q N ++ E+L  L 
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGKFTCLGFPTNQFGKQTNEKDWELLPMLA 1233604

Query: 88      YVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLPYPYDD----PFSLMTDPKLIIWS 143
               +VRPGGG+ P F +  K E NG+    +F +L+  L  P DD        +   K IIW+
Sbjct: 1233605 HVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIWT 1233784

Query: 144     PVRRSD--VAWNFEKFLIGPEGEPFRRYSRTFP 174
               PV R D      FE+F  G +     R +RT P
Sbjct: 1233785 PVTRRDQRPVTTFERFGPGKDRHRRAR*ARTSP 1233883



 Score = 69.7 bits (169), Expect = 2e-12
 Identities = 59/184 (32%), Positives = 88/184 (47%), Gaps = 1/184 (0%)
 Frame = +2

Query: 6       KSFYDLSAISLDG-EKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRRLVVL 64
               ++++D   + L G   +    +R RA L+ N AS  G   +   +L  L  R+P  L VL
Sbjct: 1870685 RTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ-LRELQALWARYPG-LAVL 1870858

Query: 65      GFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDKLP 124
                FPC+QFG QE     +I ++  Y R    Y   F + +K +VNG   HP+F YLK    
Sbjct: 1870859 AFPCDQFGGQEPGSAGDIRDA--YDR----YGVDFPVFEKSDVNGPRAHPLFTYLKK--- 1871011

Query: 125     YPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKR 184
                         T  + +    + R +V WNF K+LI  +GE  RRYS      ++  D+  
Sbjct: 1871012 ---------ATAGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVVY 1871161

Query: 185     LLKV 188
                LK+
Sbjct: 1871162 TLKL 1871173



 Score = 69.7 bits (169), Expect = 2e-12
 Identities = 68/191 (35%), Positives = 88/191 (46%), Gaps = 6/191 (3%)
 Frame = +3

Query: 2       AFIAKSFYDLSAISLDGE-KVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRR 60
               A  A S+ D +   L G   V  +T+  RA LI N AS  G T +       LQ +F  R
Sbjct: 2424435 AAAAASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR 2424611

Query: 61      LVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLK 120
               L VL FPC+QF  QE     EI  +  Y    G    TF L  K +VNG + HP+FA+LK
Sbjct: 2424612 LQVLAFPCDQF-KQEPGSPAEI--AAFYADKFG---VTFPLFAKGDVNGPDAHPLFAFLK 2424773

Query: 121     D-----KLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPT 175
               +       P P   P++               +DV WNF K+L+  +G P +RYS     
Sbjct: 2424774 EATKDQAAPVP---PWNNKGQ---------AATDVMWNFTKWLV-VDGTPTKRYSYDVKP 2424914

Query: 176     INIEPDIKRLL 186
                 IE DI   L
Sbjct: 2424915 EAIEADIAAAL 2424947



 Score = 50.1 bits (118), Expect = 2e-06
 Identities = 25/54 (46%), Positives = 34/54 (62%), Gaps = 7/54 (12%)
 Frame = +1

Query: 141     IWSPVRRS-------DVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLK 187
               IW+  R S       D+AWNFEKFLI  EG+P +RYS  F T ++  D++ LL+
Sbjct: 1233829 IWTGKRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALLE 1233990


>scaffold_34
          Length = 430012

 Score = 77.8 bits (190), Expect = 7e-15
 Identities = 48/127 (37%), Positives = 68/127 (53%), Gaps = 10/127 (7%)
 Frame = -1

Query: 8      FYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFP-RRLVVLGF 66
              F+DL +  +DG +V F+ FRG+ V + N+AS  G T   +  L +L  +F  R L ++GF
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 67     PCNQFGHQENCQNEEILNSLKYVRPGGGYQP---------TFTLVQKCEVNGQNEHPVFA 117
              PCNQF  QE     EI     + R  G   P          F L++KC+V+G    PVF+
Sbjct: 212041 PCNQFMGQEPGAESEI---KAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 118    YLKDKLP 124
              YLK + P
Sbjct: 211870 YLKREAP 211850


>scaffold_8
          Length = 1961717

 Score = 60.5 bits (145), Expect = 1e-09
 Identities = 49/171 (28%), Positives = 77/171 (45%), Gaps = 1/171 (0%)
 Frame = +3

Query: 4       IAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRR-LV 62
               +A   +DL  +   G+    + FRG A L+ N+         +F  L  +   +  + L 
Sbjct: 1062447 VATKLFDLQ-VPFKGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLR 1062623

Query: 63      VLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLKDK 122
               V  FP  Q  ++ +    E++ +  Y + G G  PT  +  K +V G   HP++ YL   
Sbjct: 1062624 VWAFPTEQGYYEPDVS--ELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL--- 1062788

Query: 123     LPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTF 173
                        +L T+P     + V R  V  NFEKFL+  +G+P RRY R F
Sbjct: 1062789 ---------ALATNP-----NGVGRLTV--NFEKFLLDGDGKPLRRYPRKF 1062893


>scaffold_972
          Length = 3742

 Score = 55.8 bits (133), Expect = 3e-08
 Identities = 33/104 (31%), Positives = 51/104 (49%)
 Frame = +3

Query: 4    IAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRRLVV 63
            +  +FYDL+ ++ DG +V F+ F G+     NVAS  G T   +  + +L   F   L V
Sbjct: 3237 VRAAFYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGDDLAV 3416

Query: 64   LGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEV 107
            + FP  +FG QE    +E +    Y R      P   L+QK ++
Sbjct: 3417 IAFPTGEFGGQE-LPTDEAIAKFAYDRVKFPAAPRGVLLQKADL 3545


>scaffold_69
          Length = 78151

 Score = 51.2 bits (121), Expect = 7e-07
 Identities = 30/89 (33%), Positives = 44/89 (49%), Gaps = 1/89 (1%)
 Frame = +2

Query: 33    IENVASLXGTTTRDFTQLNELQCRFPRR-LVVLGFPCNQFGHQENCQNEEILNSLKYVRP 91
             I +  S  G T   ++Q+ EL        L +L FPCNQFG+QE    E +    K    
Sbjct: 68663 ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK---- 68830

Query: 92    GGGYQPTFTLVQKCEVNGQNEHPVFAYLK 120
                Y   FT+++K  V+G   HPV+ +L+
Sbjct: 68831 -SAYGADFTMMEKVAVHGPRAHPVYNFLR 68914



 Score = 40.0 bits (92), Expect = 0.002
 Identities = 19/40 (47%), Positives = 26/40 (65%)
 Frame = +3

Query: 4     IAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTT 43
             +A SFYDL A +  G  + F+ F+G+ VL+ NVAS  G T
Sbjct: 68376 VASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKT 68495


>scaffold_23
          Length = 674037

 Score = 34.3 bits (77), Expect = 0.093
 Identities = 18/38 (47%), Positives = 23/38 (60%)
 Frame = -1

Query: 7      SFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTT 44
              SF+DL   +  GE VDF T +G+ VL  NVA L   T+
Sbjct: 120669 SFFDLKGRTNAGEAVDFATHKGKCVLAVNVARL*DVTS 120556


>scaffold_2
          Length = 3465789

 Score = 33.1 bits (74), Expect = 0.21
 Identities = 30/115 (26%), Positives = 45/115 (39%), Gaps = 2/115 (1%)
 Frame = -3

Query: 8      FYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPR--RLVVLG 65
              FY L A +  G    F+    R  +I NV         +   L  +         + +L 
Sbjct: 153661 FYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQILA 153482

Query: 66     FPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPVFAYLK 120
              FP ++FG +   Q          V P      +F L+ K  V+G + HPVF YL+
Sbjct: 153481 FPSDEFGPKFTDQEFVDFFVSCGVHPNDA---SFRLMAKVRVDGPDAHPVFKYLR 153326



 Score = 27.7 bits (60), Expect = 8.7
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = -2

Query: 144     PVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEP 180
               P   +D+A  + +FL    G+PF  YSR F + +  P
Sbjct: 1754522 PALTADMAGLYGRFLNWTRGKPFGSYSRAFGSASRRP 1754412


>scaffold_19
          Length = 762245

 Score = 28.1 bits (61), Expect = 6.6
 Identities = 15/29 (51%), Positives = 17/29 (58%)
 Frame = -3

Query: 143    SPVRRSDVAWNFEKFLIGPEGEPFRRYSR 171
              +P RRSDVAW F +F     GE F   SR
Sbjct: 357858 TPRRRSDVAWVFRRF----SGESFADISR 357784


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.323    0.141    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,855,858
Number of Sequences: 1185
Number of extensions: 205868
Number of successful extensions: 662
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 483
Number of HSP's gapped (non-prelim): 336
length of query: 190
length of database: 18,886,859
effective HSP length: 98
effective length of query: 92
effective length of database: 18,770,729
effective search space: 1726907068
effective search space used: 1726907068
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000006_1.0 # Protein # Glutathione peroxidase 3 (GPx3) #
Homo sapiens # Complete
         (226 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             89   4e-18
scaffold_34                                                            61   1e-09
scaffold_69                                                            49   7e-06
scaffold_8                                                             48   8e-06
scaffold_972                                                           41   0.001
scaffold_5                                                             29   5.3  
scaffold_31                                                            28   9.1  

>scaffold_6
          Length = 2755913

 Score = 89.0 bits (219), Expect = 4e-18
 Identities = 57/142 (40%), Positives = 79/142 (55%), Gaps = 11/142 (7%)
 Frame = +2

Query: 61      GKYVLFVNVASYXGLTGQ-YIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTL 119
               G+ VL  NVAS  G T + + ++N L E+ A      LGFP NQFGKQ   ++ E+LP L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 120     KYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSC-PPTSELLG-------TSDRLFW 171
                +VRPGGGFVPNF +F K + NGE     + FL++S   P+ +  G       ++  + W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 172     EPMKVHDIR--WNFEKFLVGPD 191
                P+   D R    FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 70.9 bits (172), Expect = 1e-12
 Identities = 57/172 (33%), Positives = 76/172 (44%), Gaps = 2/172 (1%)
 Frame = +3

Query: 42      EYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQYIELNALQEELAPFG--LVILGF 99
               ++    + G   +    YA +  L  N AS  G+T Q     ALQ   A FG  L +L F
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQ---AKFGDRLQVLAF 2424629

Query: 100     PCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPT 159
               PC+QF KQEPG  +EI            F   F LF KGDVNG      + FLK +   T
Sbjct: 2424630 PCDQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEA---T 2424782

Query: 160     SELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDI 211
                +          +     D+ WNF K+LV  DG P  R+ +      ++ DI
Sbjct: 2424783 KDQAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADI 2424935



 Score = 70.5 bits (171), Expect = 2e-12
 Identities = 52/174 (29%), Positives = 83/174 (47%)
 Frame = +2

Query: 39      TIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQYIELNALQEELAPFGLVILG 98
               T +++  + + G   +   +Y  +  L VN AS  G   Q  EL AL       GL +L 
Sbjct: 1870688 TYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLA 1870861

Query: 99      FPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPP 158
               FPC+QFG QEPG   +I     Y R G     +F +FEK DVNG +    +T+LK +   
Sbjct: 1870862 FPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNGPRAHPLFTYLKKAT-- 1871017

Query: 159     TSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDIL 212
                         +  + +   +++WNF K+L+  DG  + R+    +  +V  D++
Sbjct: 1871018 ------AGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVV 1871158



 Score = 34.7 bits (78), Expect = 0.097
 Identities = 13/34 (38%), Positives = 21/34 (61%)
 Frame = +1

Query: 178     DIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDI 211
               D+ WNFEKFL+  +G P+ R+      ++V  D+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADV 1233975


>scaffold_34
          Length = 430012

 Score = 61.2 bits (147), Expect = 1e-09
 Identities = 53/163 (32%), Positives = 72/163 (44%), Gaps = 8/163 (4%)
 Frame = -1

Query: 38     GTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQYIE-LNALQEELAPFGLVI 96
              G  ++  +  IDG E + F  + G+ V   N+AS  G T  + + L  L  + +  GL I
Sbjct: 212227 GGFHDLMSEDIDGAE-VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 97     LGFPCNQFGKQEPGENSEILPTLK---YVRPGGG---FVPNFQLFEKGDVNGEKEQKFYT 150
              +GFPCNQF  QEPG  SEI    +   ++ P  G    V  F L  K DV+G      ++
Sbjct: 212050 VGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 151    FLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNF-EKFLVGPDG 192
              +LK   P                     I WNF   FLV  DG
Sbjct: 211870 YLKREAP-------------------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 48.5 bits (114), Expect = 7e-06
 Identities = 27/75 (36%), Positives = 37/75 (49%)
 Frame = +2

Query: 79    YIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKG 138
             Y ++  L +EL   GL IL FPCNQFG QEP     +    K       +  +F + EK 
Sbjct: 68705 YSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMMEKV 68869

Query: 139   DVNGEKEQKFYTFLK 153
              V+G +    Y FL+
Sbjct: 68870 AVHGPRAHPVYNFLR 68914


>scaffold_8
          Length = 1961717

 Score = 48.1 bits (113), Expect = 8e-06
 Identities = 45/152 (29%), Positives = 65/152 (42%), Gaps = 3/152 (1%)
 Frame = +3

Query: 55      PFKQYAGKYVLFVNVASYXGLTGQYIELNALQEELAPF---GLVILGFPCNQFGKQEPGE 111
               P  ++ G   L VN+     + G+    NAL+   A +   GL +  FP  Q G  EP +
Sbjct: 1062498 PLSKFRGSAHLVVNIKMDDPVAGE--NFNALRYVAANYAEKGLRVWAFPTEQ-GYYEP-D 1062665

Query: 112     NSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFW 171
                SE++    Y + G G  P   +F+K DV G      Y +L                L  
Sbjct: 1062666 VSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLA---------------LAT 1062800

Query: 172     EPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTT 203
                P  V  +  NFEKFL+  DG P+ R+  + T
Sbjct: 1062801 NPNGVGRLTVNFEKFLLDGDGKPLRRYPRKFT 1062896


>scaffold_972
          Length = 3742

 Score = 41.2 bits (95), Expect = 0.001
 Identities = 49/166 (29%), Positives = 64/166 (38%), Gaps = 9/166 (5%)
 Frame = +3

Query: 36   ISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQ-YIELNALQEELAPFGL 94
            +    Y+   +  DG E + F Q+ GK    VNVAS  G T   Y  +  L +E     L
Sbjct: 3237 VRAAFYDLADVANDGSE-VAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DL 3410

Query: 95   VILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKF-YTFLK 153
             ++ FP  +FG QE       LPT                        E   KF Y  +K
Sbjct: 3411 AVIAFPTGEFGGQE-------LPT-----------------------DEAIAKFAYDRVK 3500

Query: 154  NSCPPTSELLGTSD--RLFWEPMKVH----DIRWNFE-KFLVGPDG 192
                P   LL  +D  R  W+ M+         WNF+ KFLV  DG
Sbjct: 3501 FPAAPRGVLLQKADLGRASWQAMQAEAGADAPAWNFKGKFLVSKDG 3638


>scaffold_5
          Length = 3000003

 Score = 28.9 bits (63), Expect = 5.3
 Identities = 16/42 (38%), Positives = 24/42 (57%)
 Frame = -2

Query: 85     LQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGG 126
              +++ELAP  L+ L  P ++   Q PG +    P L+  RPGG
Sbjct: 557438 VRQELAPGVLLHLRTPHHRRRVQRPGGHEAQAPLLQGARPGG 557313


>scaffold_31
          Length = 470201

 Score = 28.1 bits (61), Expect = 9.1
 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 2/26 (7%)
 Frame = -3

Query: 30     MDCHGGISGTIYEYGALT--IDGEEY 53
              M C+GGI  ++Y Y AL+  +DG  Y
Sbjct: 156882 MGCNGGIPSSVYSYWALSGIVDGGNY 156805


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.140    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,391,052
Number of Sequences: 1185
Number of extensions: 175039
Number of successful extensions: 576
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 45
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 423
Number of HSP's gapped (non-prelim): 256
length of query: 226
length of database: 18,886,859
effective HSP length: 100
effective length of query: 126
effective length of database: 18,768,359
effective search space: 2364813234
effective search space used: 2364813234
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000007_1.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Homo sapiens # Complete
         (197 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             88   6e-18
scaffold_34                                                            87   1e-17
scaffold_69                                                            84   8e-17
scaffold_8                                                             67   1e-11
scaffold_972                                                           57   2e-08
scaffold_2                                                             40   0.002
scaffold_39                                                            28   7.1  

>scaffold_6
          Length = 2755913

 Score = 88.2 bits (217), Expect = 6e-18
 Identities = 57/141 (40%), Positives = 80/141 (56%), Gaps = 5/141 (3%)
 Frame = +2

Query: 58      KYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIK 117
               +YR    +V N AS+ G       +L  L ARY   GL +LAFPC+QFG QEPGS  +I+
Sbjct: 1870745 RYRRRAHLVVNTASRCGFAP-QLRELQALWARYP--GLAVLAFPCDQFGGQEPGSAGDIR 1870915

Query: 118     EFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKG-----KGILGNAIKWNFTKFLIDK 172
               +    Y V F +F K  VNG  AHPL+ ++K    G     KG+  + ++WNFTK+LI  
Sbjct: 1870916 DAYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVPNKGLPRDEVQWNFTKWLI-V 1871092

Query: 173     NGCVVKRYGPMEEPLVIEKDL 193
               +G V++RY     P  +  D+
Sbjct: 1871093 DGEVLRRYSFDASPKSVLADV 1871155



 Score = 84.7 bits (208), Expect = 6e-17
 Identities = 65/170 (38%), Positives = 87/170 (51%), Gaps = 14/170 (8%)
 Frame = +3

Query: 38      ARSMHEFSAKDIDGH-MVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECG-- 94
               A S  +F+   + G   V L  Y     ++ N AS+ G T     QL    A  A+ G  
Sbjct: 2424444 AASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTP----QLGGFQALQAKFGDR 2424611

Query: 95      LRILAFPCNQFGKQEPGSNEEIKEFAAG-YNVKFDMFSKICVNGDDAHPLWKWMKIQPK- 152
               L++LAFPC+QF KQEPGS  EI  F A  + V F +F+K  VNG DAHPL+ ++K   K 
Sbjct: 2424612 LQVLAFPCDQF-KQEPGSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKD 2424788

Query: 153     ---------GKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193
                         KG     + WNFTK+L+  +G   KRY    +P  IE D+
Sbjct: 2424789 QAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADI 2424935



 Score = 49.7 bits (117), Expect = 2e-06
 Identities = 34/94 (36%), Positives = 50/94 (53%), Gaps = 6/94 (6%)
 Frame = +2

Query: 61      GFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA 120
               G V +V NVAS  G T  ++TQ+ DL  ++A      L FP NQFGKQ    + E+    
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 121     A------GYNVKFDMFSKICVNGDDAHPLWKWMK 148
               A      G+   F +F+K   NG+ A  L+K+++
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLR 1233703



 Score = 38.5 bits (88), Expect = 0.005
 Identities = 18/45 (40%), Positives = 24/45 (53%)
 Frame = +1

Query: 138     DDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGP 182
               DD   +W   +  P     +G  + WNF KFLI++ G  VKRY P
Sbjct: 1233814 DDFREIWTGKRSSPSRS--VGTDLAWNFEKFLINQEGKPVKRYSP 1233942


>scaffold_34
          Length = 430012

 Score = 87.4 bits (215), Expect = 1e-17
 Identities = 55/148 (37%), Positives = 78/148 (52%), Gaps = 13/148 (8%)
 Frame = -1

Query: 42     HEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFP 101
              H+  ++DIDG  V    +RG V  VTN+AS+ GKT  +Y  L  L A+++  GL I+ FP
Sbjct: 212218 HDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFP 212039

Query: 102    CNQFGKQEPGSNEEIKEFA------------AGYNVKFDMFSKICVNGDDAHPLWKWMKI 149
              CNQF  QEPG+  EIK FA            A     F++  K  V+G    P++ ++K 
Sbjct: 212038 CNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKR 211859

Query: 150    QPKGKGILGNAIKWNF-TKFLIDKNGCV 176
              +   K      I WNF   FL+ ++G V
Sbjct: 211858 EAPCK------ISWNFGAYFLVSRDGAV 211793


>scaffold_69
          Length = 78151

 Score = 84.3 bits (207), Expect = 8e-17
 Identities = 44/116 (37%), Positives = 63/116 (54%), Gaps = 1/116 (0%)
 Frame = +2

Query: 66    VTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYN 124
             +++  S  GKT  +Y+Q+V+LH      GL ILAFPCNQFG QEP + E + EFA + Y 
Sbjct: 68663 ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYG 68842

Query: 125   VKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRY 180
               F M  K+ V+G  AHP++ ++      +   G    WNF  + +      VK Y
Sbjct: 68843 ADFTMMEKVAVHGPRAHPVYNFL------RATTGATPSWNFGVYFVVSRSGEVKAY 68992



 Score = 34.3 bits (77), Expect = 0.099
 Identities = 24/73 (32%), Positives = 34/73 (46%)
 Frame = +3

Query: 38    ARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRI 97
             A S ++  AK   G  +  D ++G V ++TNVAS  GKT  +  +  D  +   EC  R 
Sbjct: 68379 ASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAH--EKSDSTSLQRECSAR- 68549

Query: 98    LAFPCNQFGKQEP 110
                    F KQ P
Sbjct: 68550 ------AFRKQHP 68570


>scaffold_8
          Length = 1961717

 Score = 67.4 bits (163), Expect = 1e-11
 Identities = 44/130 (33%), Positives = 67/130 (51%), Gaps = 5/130 (3%)
 Frame = +3

Query: 56      LDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEE 115
               L K+RG   +V N+         N+  L  + A YAE GLR+ AFP  Q G  EP  +E 
Sbjct: 1062501 LSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYEPDVSEL 1062677

Query: 116     IK-----EFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLI 170
               ++     +F  G      +F KI V G+ AHPL++++ +     G+    +  NF KFL+
Sbjct: 1062678 VRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLALATNPNGV--GRLTVNFEKFLL 1062851

Query: 171     DKNGCVVKRY 180
               D +G  ++RY
Sbjct: 1062852 DGDGKPLRRY 1062881


>scaffold_972
          Length = 3742

 Score = 56.6 bits (135), Expect = 2e-08
 Identities = 43/135 (31%), Positives = 62/135 (45%), Gaps = 3/135 (2%)
 Frame = +3

Query: 50   DGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQE 109
            DG  V   ++ G VC   NVAS  G T   Y  +  L   + +  L ++AFP  +FG QE
Sbjct: 3276 DGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIAFPTGEFGGQE 3452

Query: 110  PGSNEEIKEFAAGYNVKFDMFSK--ICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFT- 166
              ++E I +FA    VKF    +  +    D     W+ M+ +        +A  WNF  
Sbjct: 3453 LPTDEAIAKFAYD-RVKFPAAPRGVLLQKADLGRASWQAMQAEAG-----ADAPAWNFKG 3614

Query: 167  KFLIDKNGCVVKRYG 181
            KFL+ K+G V    G
Sbjct: 3615 KFLVSKDGAVTDASG 3659


>scaffold_2
          Length = 3465789

 Score = 40.0 bits (92), Expect = 0.002
 Identities = 20/61 (32%), Positives = 39/61 (63%), Gaps = 5/61 (8%)
 Frame = -3

Query: 94     GLRILAFPCNQFGKQEPGSNEEIKEFAAGYNV-----KFDMFSKICVNGDDAHPLWKWMK 148
              G++ILAFP ++FG +   +++E  +F     V      F + +K+ V+G DAHP++K+++
Sbjct: 153499 GVQILAFPSDEFGPK--FTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLR 153326

Query: 149    I 149
              +
Sbjct: 153325 L 153323


>scaffold_39
          Length = 370504

 Score = 28.1 bits (61), Expect = 7.1
 Identities = 12/39 (30%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
 Frame = -3

Query: 129   MFSKICVNGDDAHPLWKWMKIQPKGKG--ILGNAIKWNF 165
             +F+   +  ++ H LW+W+++ P  +G  IL  A +W F
Sbjct: 66197 IFTLCSIIVNNFHKLWRWLELFPNARGGFIL*RARRWVF 66081


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.324    0.140    0.457 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,739,614
Number of Sequences: 1185
Number of extensions: 161450
Number of successful extensions: 586
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 471
Number of HSP's gapped (non-prelim): 263
length of query: 197
length of database: 18,886,859
effective HSP length: 99
effective length of query: 98
effective length of database: 18,769,544
effective search space: 1839415312
effective search space used: 1839415312
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000008_1.0 # Protein # Glutathione peroxidase 5 (GPx5) #
Homo sapiens # Complete
         (221 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             89   4e-18
scaffold_34                                                            59   4e-09
scaffold_69                                                            50   2e-06
scaffold_8                                                             43   3e-04
scaffold_972                                                           40   0.003

>scaffold_6
          Length = 2755913

 Score = 89.0 bits (219), Expect = 4e-18
 Identities = 58/147 (39%), Positives = 82/147 (55%), Gaps = 12/147 (8%)
 Frame = +2

Query: 57      KQYVGKHILFVNVATYCGLTAQ-YPELNALQEELKPYGLVVLGFPCNQFGKQEPGDNKEI 115
               ++ +G+ +L  NVA+  G T + + ++N L E+        LGFP NQFGKQ    + E+
Sbjct: 1233413 RRSLGQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWEL 1233589

Query: 116     LPGLKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSE---------ILGTF 166
               LP L +VRPGGGFVP+F +F K + NGE    +F FL+ S   PS+         ++ T 
Sbjct: 1233590 LPMLAHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVIST- 1233766

Query: 167     KSISWDPVKVHDIR--WNFEKFLVGPD 191
               K+I W PV   D R    FE+F  G D
Sbjct: 1233767 KNIIWTPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 87.8 bits (216), Expect = 9e-18
 Identities = 69/197 (35%), Positives = 98/197 (49%), Gaps = 4/197 (2%)
 Frame = +2

Query: 24      KQEKMKMDCHKDEK-GTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYPEL 82
               K E    D  K E+  T +D+E + L     ++  +Y  +  L VN A+ CG   Q  EL
Sbjct: 1870640 KLEAAAHDARKQERLRTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLREL 1870819

Query: 83      NALQEELKPYGLVVLGFPCNQFGKQEPGDNKEILPGLKYVRPGGGFVPSFQLFEKGDVNG 142
                AL       GL VL FPC+QFG QEPG   +I     Y R G      F +FEK DVNG
Sbjct: 1870820 QALWARYP--GLAVLAFPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNG 1870975

Query: 143     EKEQKVFSFLKHSC---PHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWS 199
                +   +F++LK +    P P+      K +  D     +++WNF K+L+  DG  + R+S
Sbjct: 1870976 PRAHPLFTYLKKATAGQPVPN------KGLPRD-----EVQWNFTKWLI-VDGEVLRRYS 1871119

Query: 200     HRATVSSVKTDILAYLK 216
                 A+  SV  D++  LK
Sbjct: 1871120 FDASPKSVLADVVYTLK 1871170



 Score = 79.7 bits (195), Expect = 3e-15
 Identities = 59/174 (33%), Positives = 81/174 (46%)
 Frame = +3

Query: 42      DYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYPELNALQEELKPYGLVVLGFPC 101
               D+    L     V+   Y  +  L  N A+ CG+T Q     ALQ +     L VL FPC
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQAKFGDR-LQVLAFPC 2424635

Query: 102     NQFGKQEPGDNKEILPGLKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSE 161
               +QF KQEPG   EI            F  +F LF KGDVNG     +F+FLK +    + 
Sbjct: 2424636 DQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAA 2424797

Query: 162     ILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYL 215
                +  + +         D+ WNF K+LV  DG P  R+S+     +++ DI A L
Sbjct: 2424798 PVPPWNN---KGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAAL 2424947



 Score = 38.9 bits (89), Expect = 0.005
 Identities = 17/39 (43%), Positives = 24/39 (61%)
 Frame = +1

Query: 178     DIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLK 216
               D+ WNFEKFL+  +G PV R+S     + V  D+ A L+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALLE 1233990



 Score = 28.9 bits (63), Expect = 5.1
 Identities = 17/80 (21%), Positives = 35/80 (43%)
 Frame = +3

Query: 54     VSFKQYVGKHILFVNVATYCGLTAQYPELNALQEELKPYGLVVLGFPCNQFGKQEPGDNK 113
              V+ +++   +++  N+   C +    P   A+Q ++          PC ++G    GD+K
Sbjct: 478488 VAAEEHGVPNVVHTNIDIECAMENYEPSFIAIQPKMT--------VPCMKYGDDVIGDSK 478643

Query: 114    EILPGLKYVRPGGGFVPSFQ 133
              +I+  L    P     P+ Q
Sbjct: 478644 DIMYYLSEKHPDAALYPAEQ 478703


>scaffold_34
          Length = 430012

 Score = 59.3 bits (142), Expect = 4e-09
 Identities = 49/147 (33%), Positives = 64/147 (43%), Gaps = 8/147 (5%)
 Frame = -1

Query: 54     VSFKQYVGKHILFVNVATYCGLT-AQYPELNALQEELKPYGLVVLGFPCNQFGKQEPGDN 112
              V F  + G+ +   N+A+  G T + Y  L  L  +    GL ++GFPCNQF  QEPG  
Sbjct: 212182 VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFPCNQFMGQEPGAE 212003

Query: 113    KEILPGLK---YVRPGGG---FVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEILGTF 166
               EI    +   ++ P  G    V +F L  K DV+G     VFS+LK   P         
Sbjct: 212002 SEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKREAP--------- 211850

Query: 167    KSISWDPVKVHDIRWNF-EKFLVGPDG 192
                          I WNF   FLV  DG
Sbjct: 211849 ----------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 50.1 bits (118), Expect = 2e-06
 Identities = 27/77 (35%), Positives = 40/77 (51%)
 Frame = +2

Query: 77    AQYPELNALQEELKPYGLVVLGFPCNQFGKQEPGDNKEILPGLKYVRPGGGFVPSFQLFE 136
             A Y ++  L +EL   GL +L FPCNQFG QEP   + +    K       +   F + E
Sbjct: 68699 AHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMME 68863

Query: 137   KGDVNGEKEQKVFSFLK 153
             K  V+G +   V++FL+
Sbjct: 68864 KVAVHGPRAHPVYNFLR 68914


>scaffold_8
          Length = 1961717

 Score = 42.7 bits (99), Expect = 3e-04
 Identities = 34/111 (30%), Positives = 52/111 (46%)
 Frame = +3

Query: 93      GLVVLGFPCNQFGKQEPGDNKEILPGLKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFL 152
               GL V  FP  Q G  EP D  E++    Y + G G  P+  +F+K DV G     ++ +L
Sbjct: 1062615 GLRVWAFPTEQ-GYYEP-DVSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL 1062788

Query: 153     KHSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRAT 203
                              +++ +P  V  +  NFEKFL+  DG P+ R+  + T
Sbjct: 1062789 ---------------ALATNPNGVGRLTVNFEKFLLDGDGKPLRRYPRKFT 1062896



 Score = 29.6 bits (65), Expect = 3.0
 Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 5/64 (7%)
 Frame = +2

Query: 158     HPSEILGTFKSISWDPVK-----VHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDIL 212
               H   + G+ K  SW  +K     V    W F++ L        +RW H     S K+D+L
Sbjct: 1315280 HADTLAGSSKKTSWAGLKGPLVAVAGPTWTFKERLAD------LRWDHGPLPRSAKSDVL 1315441

Query: 213     AYLK 216
               A L+
Sbjct: 1315442 AALE 1315453


>scaffold_972
          Length = 3742

 Score = 39.7 bits (91), Expect = 0.003
 Identities = 46/159 (28%), Positives = 61/159 (38%), Gaps = 3/159 (1%)
 Frame = +3

Query: 37   KGTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQ-YPELNALQEELKPYGLV 95
            +   YD   +A N    V+F Q+ GK    VNVA+  G T   Y  +  L +E     L 
Sbjct: 3240 RAAFYDLADVA-NDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLA 3413

Query: 96   VLGFPCNQFGKQE-PGDNKEILPGLKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKH 154
            V+ FP  +FG QE P D  E +    Y R      P   L +K D+     Q + +    
Sbjct: 3414 VIAFPTGEFGGQELPTD--EAIAKFAYDRVKFPAAPRGVLLQKADLGRASWQAMQAEAGA 3587

Query: 155  SCPHPSEILGTFKSISWDPVKVHDIRWNFE-KFLVGPDG 192
              P                       WNF+ KFLV  DG
Sbjct: 3588 DAP----------------------AWNFKGKFLVSKDG 3638


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.138    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,318,939
Number of Sequences: 1185
Number of extensions: 174418
Number of successful extensions: 630
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 515
Number of HSP's gapped (non-prelim): 263
length of query: 221
length of database: 18,886,859
effective HSP length: 100
effective length of query: 121
effective length of database: 18,768,359
effective search space: 2270971439
effective search space used: 2270971439
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000009_1.0 # Protein # Glutathione peroxidase 6 (GPx6) #
Homo sapiens # Complete
         (221 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             74   1e-13
scaffold_34                                                            60   2e-09
scaffold_69                                                            55   9e-08
scaffold_8                                                             43   3e-04
scaffold_972                                                           38   0.011
scaffold_13                                                            30   1.8  
scaffold_2                                                             29   3.9  

>scaffold_6
          Length = 2755913

 Score = 73.9 bits (180), Expect = 1e-13
 Identities = 52/142 (36%), Positives = 73/142 (51%), Gaps = 11/142 (7%)
 Frame = +2

Query: 61      GKHVLFVNVAAYXGLAAQ-YPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGL 119
               G+ VL  NVA+  G   + + ++N L E+        L FP NQFGKQ    + E+L  L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 120     KYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSC-PPTSDLLG-------SSSQLFW 171
                +V PG GFVP+F +F K + NGE    +F FL++S   P+ D  G       S+  + W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 172     EPMKVHDIR--WNFEKFLVGPD 191
                P+   D R    FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 73.2 bits (178), Expect = 2e-13
 Identities = 53/178 (29%), Positives = 87/178 (48%)
 Frame = +2

Query: 39      TIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLAAQYPELNALQEELKNFGVIVLA 98
               T +++  + L G   +   ++  +  L VN A+  G A Q  EL AL       G+ VLA
Sbjct: 1870688 TYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLA 1870861

Query: 99      FPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPP 158
               FPC+QFG QEPG+  +I            +   F +FEK DVNG +   +FT+LK +   
Sbjct: 1870862 FPCDQFGGQEPGSAGDIRDAY------DRYGVDFPVFEKSDVNGPRAHPLFTYLKKAT-- 1871017

Query: 159     TSDLLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMHWFHQAPVSTVKSDILEYLK 216
                     +   +  + +   +++WNF K+L+  DG  +  +   A   +V +D++  LK
Sbjct: 1871018 ------AGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVVYTLK 1871170



 Score = 72.8 bits (177), Expect = 3e-13
 Identities = 54/170 (31%), Positives = 78/170 (45%)
 Frame = +3

Query: 42      EYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLAAQYPELNALQEELKNFGVIVLAFPC 101
               ++    L G   +    +A +  L  N A+  G+  Q     ALQ +  +  + VLAFPC
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQAKFGD-RLQVLAFPC 2424635

Query: 102     NQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPPTSD 161
               +QF KQEPG+ +EI            F  +F LF KGDVNG     +F FLK +   T D
Sbjct: 2424636 DQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEA---TKD 2424788

Query: 162     LLGSSSQLFWEPMKVHDIRWNFEKFLVGPDGVPVMHWFHQAPVSTVKSDI 211
                         +     D+ WNF K+LV  DG P   + +      +++DI
Sbjct: 2424789 QAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADI 2424935



 Score = 32.3 bits (72), Expect = 0.46
 Identities = 13/34 (38%), Positives = 20/34 (58%)
 Frame = +1

Query: 178     DIRWNFEKFLVGPDGVPVMHWFHQAPVSTVKSDI 211
               D+ WNFEKFL+  +G PV  +      + V +D+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADV 1233975


>scaffold_34
          Length = 430012

 Score = 60.1 bits (144), Expect = 2e-09
 Identities = 51/168 (30%), Positives = 73/168 (43%), Gaps = 8/168 (4%)
 Frame = -1

Query: 38     GTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLA-AQYPELNALQEELKNFGVIV 96
              G  ++  +  ++G E ++F  F G+ V   N+A+  G   + Y  L  L  +    G+ +
Sbjct: 212227 GGFHDLMSEDIDGAE-VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 97     LAFPCNQFGKQEPGTNSEILLGLK---YVCPGSG---FVPSFQLFEKGDVNGEKEQKVFT 150
              + FPCNQF  QEPG  SEI    +   ++ P  G    V +F L  K DV+G     VF+
Sbjct: 212050 VGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 151    FLKNSCPPTSDLLGSSSQLFWEPMKVHDIRWNF-EKFLVGPDGVPVMH 197
              +LK   P                     I WNF   FLV  DG    H
Sbjct: 211870 YLKREAP-------------------CKISWNFGAYFLVSRDGAVEAH 211784


>scaffold_69
          Length = 78151

 Score = 54.7 bits (130), Expect = 9e-08
 Identities = 30/78 (38%), Positives = 43/78 (55%)
 Frame = +2

Query: 76    AAQYPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLF 135
             AA Y ++  L +EL + G+ +LAFPCNQFG QEP T   +    K     S +   F + 
Sbjct: 68696 AAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMM 68860

Query: 136   EKGDVNGEKEQKVFTFLK 153
             EK  V+G +   V+ FL+
Sbjct: 68861 EKVAVHGPRAHPVYNFLR 68914


>scaffold_8
          Length = 1961717

 Score = 42.7 bits (99), Expect = 3e-04
 Identities = 43/144 (29%), Positives = 62/144 (43%), Gaps = 3/144 (2%)
 Frame = +3

Query: 58      QFAGKHVLFVNVAAYXGLAAQYPELNALQEELKNF---GVIVLAFPCNQFGKQEPGTNSE 114
               +F G   L VN+     +A +    NAL+    N+   G+ V AFP  Q G  EP   SE
Sbjct: 1062507 KFRGSAHLVVNIKMDDPVAGE--NFNALRYVAANYAEKGLRVWAFPTEQ-GYYEPDV-SE 1062674

Query: 115     ILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPM 174
               ++    Y   G G  P+  +F+K DV G     ++ +L                L   P 
Sbjct: 1062675 LVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLA---------------LATNPN 1062809

Query: 175     KVHDIRWNFEKFLVGPDGVPVMHW 198
                V  +  NFEKFL+  DG P+  +
Sbjct: 1062810 GVGRLTVNFEKFLLDGDGKPLRRY 1062881


>scaffold_972
          Length = 3742

 Score = 37.7 bits (86), Expect = 0.011
 Identities = 47/164 (28%), Positives = 65/164 (39%), Gaps = 7/164 (4%)
 Frame = +3

Query: 36   VTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLAAQYPELNALQEELKNFG-- 93
            V    Y+   +  +G E + F QF GK    VNVA+  G  A  P    +++    FG  
Sbjct: 3237 VRAAFYDLADVANDGSE-VAFSQFEGKVCYAVNVASA*G--ATRPGYKLMKQLSDEFGDD 3407

Query: 94   VIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLK 153
            + V+AFP  +FG QE  T+ E +    Y        P   L +K D              
Sbjct: 3408 LAVIAFPTGEFGGQELPTD-EAIAKFAYDRVKFPAAPRGVLLQKAD-------------- 3542

Query: 154  NSCPPTSDLLGSSSQLFWEPMKVH----DIRWNFE-KFLVGPDG 192
                     LG +S   W+ M+         WNF+ KFLV  DG
Sbjct: 3543 ---------LGRAS---WQAMQAEAGADAPAWNFKGKFLVSKDG 3638


>scaffold_13
          Length = 1338096

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 25/94 (26%), Positives = 37/94 (39%), Gaps = 23/94 (24%)
 Frame = -3

Query: 138    GDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPM---------------KVHDIRWN 182
              G  + E EQ+V+  +  SCP  +  + S+   F  PM               K+ D+  +
Sbjct: 701473 GAQSTESEQRVYVAIVCSCPRNASDVYSTKSRFSSPMKGAYWTLTWWSTPGLKIDDLTRS 701294

Query: 183    FEKFLVGPDGVP--------VMHWFHQAPVSTVK 208
              F  F+    GVP        +  WF  A   TVK
Sbjct: 701293 FAAFVRACVGVPSARFRMSLLPRWFDDATTFTVK 701192


>scaffold_2
          Length = 3465789

 Score = 29.3 bits (64), Expect = 3.9
 Identities = 34/139 (24%), Positives = 54/139 (38%), Gaps = 2/139 (1%)
 Frame = -3

Query: 17     GFAQQTLKPQNRKVDCNKGVTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVAAYXGLA 76
              GF    L+    K       T   Y+  A T  G  +  F     +  + VNV  +   A
Sbjct: 153730 GFRTDRLEELTVKEAAYHTKTSRFYKLVAHTARGWPF-PFDDLHHRKCIIVNVGIHGPDA 153554

Query: 77     AQ-YPELNALQEELKNF-GVIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQL 134
               +    L  + +++    GV +LAFP ++FG   P    +  +     C       SF+L
Sbjct: 153553 REEVAALKRISDDISPVDGVQILAFPSDEFG---PKFTDQEFVDFFVSCGVHPNDASFRL 153383

Query: 135    FEKGDVNGEKEQKVFTFLK 153
                K  V+G     VF +L+
Sbjct: 153382 MAKVRVDGPDAHPVFKYLR 153326


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.322    0.139    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,313,469
Number of Sequences: 1185
Number of extensions: 186318
Number of successful extensions: 464
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 18
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 389
Number of HSP's gapped (non-prelim): 166
length of query: 221
length of database: 18,886,859
effective HSP length: 100
effective length of query: 121
effective length of database: 18,768,359
effective search space: 2270971439
effective search space used: 2270971439
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000010_1.0 # Protein # Glutathione peroxidase 7 (GPx7) #
Homo sapiens # Complete
         (187 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_69                                                            90   2e-18
scaffold_6                                                             85   6e-17
scaffold_34                                                            82   4e-16
scaffold_8                                                             64   1e-10
scaffold_972                                                           62   3e-10
scaffold_2                                                             47   1e-05

>scaffold_69
          Length = 78151

 Score = 89.7 bits (221), Expect = 2e-18
 Identities = 42/102 (41%), Positives = 56/102 (54%)
 Frame = +2

Query: 58    GFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSK 117
             G T  HY  + +L  +LG     +LAFPCNQFG QEP + + +  FA+  Y   F M  K
Sbjct: 68687 GKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEK 68866

Query: 118   IAVTGTGAHPAFKYLAQTSGKEPTWNFWKYLVAPDGKVVGAW 159
             +AV G  AHP + +L  T+G  P+WNF  Y V      V A+
Sbjct: 68867 VAVHGPRAHPVYNFLRATTGATPSWNFGVYFVVSRSGEVKAY 68992



 Score = 35.0 bits (79), Expect = 0.053
 Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
 Frame = +3

Query: 25    FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRA-LQQLQRDLGPHHF 79
             FYD KA    G  ++ + ++G V L+ NVAS  G T  H ++    LQR+     F
Sbjct: 68388 FYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAHEKSDSTSLQRECSARAF 68555


>scaffold_6
          Length = 2755913

 Score = 84.7 bits (208), Expect = 6e-17
 Identities = 62/157 (39%), Positives = 81/157 (51%), Gaps = 13/157 (8%)
 Frame = +2

Query: 25      FYDFKAVNIRGKL-VSLEKYRGSVSLVVNVASECGFTDQHYRALQQL-QRDLGPHHFNVL 82
               ++DF+ V + G   ++  +YR    LVVN AS CGF  Q  R LQ L  R  G     VL
Sbjct: 1870691 YWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ-LRELQALWARYPG---LAVL 1870858

Query: 83      AFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQTSGKEPT- 141
               AFPC+QFG QEP S  +I     R Y V FP+F K  V G  AHP F YL + +  +P  
Sbjct: 1870859 AFPCDQFGGQEPGSAGDIRDAYDR-YGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVP 1871035

Query: 142     ----------WNFWKYLVAPDGKVVGAWDPTVSVEEV 168
                         WNF K+L+  DG+V+  +    S + V
Sbjct: 1871036 NKGLPRDEVQWNFTKWLIV-DGEVLRRYSFDASPKSV 1871143



 Score = 79.0 bits (193), Expect = 3e-15
 Identities = 57/156 (36%), Positives = 74/156 (47%), Gaps = 16/156 (10%)
 Frame = +3

Query: 35      GKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEP 94
               G  V+L  Y     L+ N AS+CG T Q     Q LQ   G     VLAFPC+QF +QEP
Sbjct: 2424486 GGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR-LQVLAFPCDQF-KQEP 2424656

Query: 95      DSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQTS-------------GKEPT 141
                S  EI +F    + V+FP+F+K  V G  AHP F +L + +             G+  T
Sbjct: 2424657 GSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPPWNNKGQAAT 2424836

Query: 142     ---WNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITA 174
                  WNF K+LV  DG     +   V  E +   I A
Sbjct: 2424837 DVMWNFTKWLVV-DGTPTKRYSYDVKPEAIEADIAA 2424941



 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 32/98 (32%), Positives = 45/98 (45%), Gaps = 5/98 (5%)
 Frame = +2

Query: 45      GSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFA 104
               G V LV NVAS  G T + +  +  L        F  L FP NQFG+Q  + + E+    
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 105     RRT-----YSVSFPMFSKIAVTGTGAHPAFKYLAQTSG 137
                       +  +FP+F+K    G GA   FK+L  + G
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLG 1233715



 Score = 30.4 bits (67), Expect = 1.3
 Identities = 13/44 (29%), Positives = 24/44 (54%)
 Frame = +1

Query: 133     AQTSGKEPTWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITALV 176
               +++ G +  WNF K+L+  +GK V  + P     +V   + AL+
Sbjct: 1233856 SRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALL 1233987


>scaffold_34
          Length = 430012

 Score = 82.0 bits (201), Expect = 4e-16
 Identities = 52/164 (31%), Positives = 79/164 (48%), Gaps = 12/164 (7%)
 Frame = -1

Query: 25     FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAF 84
              F+D  + +I G  V    +RG V  V N+AS  G T+ HY+ L +L          ++ F
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 85     PCNQFGQQEPDSNKEIESFARRTYSV-----------SFPMFSKIAVTGTGAHPAFKYLA 133
              PCNQF  QEP +  EI++FAR    +           +F +  K  V+G    P F YL 
Sbjct: 212041 PCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLK 211862

Query: 134    QTSGKEPTWNFWKY-LVAPDGKVVGAWDPTVSVEEVRPQITALV 176
              + +  + +WNF  Y LV+ DG V           ++ P++ AL+
Sbjct: 211861 REAPCKISWNFGAYFLVSRDGAVEA--HENAHPRDLAPRVEALL 211736


>scaffold_8
          Length = 1961717

 Score = 63.9 bits (154), Expect = 1e-10
 Identities = 46/133 (34%), Positives = 64/133 (48%), Gaps = 9/133 (6%)
 Frame = +3

Query: 31      VNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFG 90
               V  +GK   L K+RGS  LVVN+  +     +++ AL+ +  +       V AFP  Q G
Sbjct: 1062474 VPFKGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-G 1062650

Query: 91      QQEPDSN-----KEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQTSGKEP----T 141
                 EPD +     K    F   TY  +  +F KI V G  AHP ++YLA  +        T
Sbjct: 1062651 YYEPDVSELVRAKAYSQFGFGTYPTAV-VFDKIDVIGNTAHPLYRYLALATNPNGVGRLT 1062827

Query: 142     WNFWKYLVAPDGK 154
                NF K+L+  DGK
Sbjct: 1062828 VNFEKFLLDGDGK 1062866


>scaffold_972
          Length = 3742

 Score = 62.4 bits (150), Expect = 3e-10
 Identities = 51/158 (32%), Positives = 75/158 (47%), Gaps = 6/158 (3%)
 Frame = +3

Query: 25   FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAF 84
            FYD   V   G  V+  ++ G V   VNVAS  G T   Y+ ++QL  + G     V+AF
Sbjct: 3249 FYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFG-DDLAVIAF 3425

Query: 85   PCNQFGQQEPDSNKEIESFARRTYSVSFP-----MFSKIAVTGTGAHPAFKYLAQTSGKE 139
            P  +FG QE  +++ I  FA     V FP     +  + A  G  +  A +  A+     
Sbjct: 3426 PTGEFGGQELPTDEAIAKFAYD--RVKFPAAPRGVLLQKADLGRASWQAMQ--AEAGADA 3593

Query: 140  PTWNF-WKYLVAPDGKVVGAWDPTVSVEEVRPQITALV 176
            P WNF  K+LV+ DG V  A      V+++   + AL+
Sbjct: 3594 PAWNFKGKFLVSKDGAVTDA----SGVDDIAAAVKALL 3695


>scaffold_2
          Length = 3465789

 Score = 47.0 bits (110), Expect = 1e-05
 Identities = 34/118 (28%), Positives = 49/118 (41%), Gaps = 3/118 (2%)
 Frame = -3

Query: 25     FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGP-HHFNVLA 83
              FY   A   RG     +       ++VNV        +   AL+++  D+ P     +LA
Sbjct: 153661 FYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQILA 153482

Query: 84     FPCNQFGQQEPDSN--KEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQTSGKE 139
              FP ++FG +  D        S        SF + +K+ V G  AHP FKYL   S  E
Sbjct: 153481 FPSDEFGPKFTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLRLMSNTE 153308


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,121,918
Number of Sequences: 1185
Number of extensions: 144806
Number of successful extensions: 617
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 50
Number of HSP's successfully gapped in prelim test: 30
Number of HSP's that attempted gapping in prelim test: 406
Number of HSP's gapped (non-prelim): 386
length of query: 187
length of database: 18,886,859
effective HSP length: 98
effective length of query: 89
effective length of database: 18,770,729
effective search space: 1670594881
effective search space used: 1670594881
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000011_1.0 # Protein # Glutathione peroxidase 8 (GPx8) #
Homo sapiens # Complete
         (209 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_69                                                            78   9e-15
scaffold_6                                                             77   1e-14
scaffold_34                                                            69   5e-12
scaffold_8                                                             57   2e-08
scaffold_972                                                           53   3e-07

>scaffold_69
          Length = 78151

 Score = 77.8 bits (190), Expect = 9e-15
 Identities = 38/108 (35%), Positives = 52/108 (48%)
 Frame = +2

Query: 82    TDRNYLGLKELHKEFGPSHFSVLAFPCNQFGESEPRPSKEVESFARKNYGVTFPIFHKIK 141
             T  +Y  + ELH E G     +LAFPCNQFG  EP   + V  FA+  YG  F +  K+ 
Sbjct: 68693 TAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEKVA 68872

Query: 142   ILGSEGEPAFRFLVDSSKKEPRWNFWKYLVNPEGQVVKFWKPEEPIEV 189
             + G    P + FL  ++   P WNF  Y V      VK +    P ++
Sbjct: 68873 VHGPRAHPVYNFLRATTGATPSWNFGVYFVVSRSGEVKAYPDVAPAQL 69016



 Score = 35.8 bits (81), Expect = 0.038
 Identities = 16/33 (48%), Positives = 23/33 (69%)
 Frame = +3

Query: 45    NSFYAFEVKDAKGRTVSLEKYKGKVSLVVNVAS 77
             +SFY  + K A G T++ + +KGKV L+ NVAS
Sbjct: 68382 SSFYDLKAKTAAGATLTFDAFKGKVVLLTNVAS 68480


>scaffold_6
          Length = 2755913

 Score = 77.4 bits (189), Expect = 1e-14
 Identities = 53/156 (33%), Positives = 76/156 (48%), Gaps = 16/156 (10%)
 Frame = +3

Query: 57      GRTVSLEKYKGKVSLVVNVASDCQLTDRNYLGLKELHKEFGPSHFSVLAFPCNQFGESEP 116
               G  V+L  Y  +  L+ N AS C +T +   G + L  +FG     VLAFPC+QF + EP
Sbjct: 2424486 GGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFG-DRLQVLAFPCDQF-KQEP 2424656

Query: 117     RPSKEVESFARKNYGVTFPIFHKIKILGSEGEPAFRFLVDSSKKEP-------------- 162
                   E+ +F    +GVTFP+F K  + G +  P F FL +++K +               
Sbjct: 2424657 GSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPPWNNKGQAAT 2424836

Query: 163     --RWNFWKYLVNPEGQVVKFWKPEEPIEVIRPDIAA 196
                  WNF K+LV  +G   K +  +   E I  DIAA
Sbjct: 2424837 DVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAA 2424941



 Score = 68.9 bits (167), Expect = 4e-12
 Identities = 52/201 (25%), Positives = 91/201 (45%), Gaps = 38/201 (18%)
 Frame = +2

Query: 43      KINSFYAFE-VKDAKGRTVSLEKYKGKVSLVVNVASDCQLTDRNYLGLKELHKEFGP-SH 100
               ++ +++ FE V    G  ++  +Y+ +  LVVN AS C    +    L+EL   +     
Sbjct: 1870679 RLRTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ----LRELQALWARYPG 1870846

Query: 101     FSVLAFPCNQFGESEPRPSKEVESFARKNYGVTFPIFHKIKILGSEGEPAFRFLVDSSKK 160
                +VLAFPC+QFG  EP  + ++   A   YGV FP+F K  + G    P F +L  ++  
Sbjct: 1870847 LAVLAFPCDQFGGQEPGSAGDIRD-AYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAG 1871023

Query: 161     EP-----------RWNFWKYLV-------------NPEG------------QVVKFWKPE 184
               +P           +WNF K+L+             +P+             +V++   P 
Sbjct: 1871024 QPVPNKGLPRDEVQWNFTKWLIVDGEVLRRYSFDASPKSVLADVVYTLKLKEVIEEAPPA 1871203

Query: 185     EPIEVIRPDIAALVRQVIIKK 205
                P+EV +P+   + +  ++ K
Sbjct: 1871204 RPVEVEKPEAVDVPKPAVVPK 1871266



 Score = 42.7 bits (99), Expect = 3e-04
 Identities = 38/120 (31%), Positives = 54/120 (45%), Gaps = 14/120 (11%)
 Frame = +2

Query: 52      VKDAKGRTVSL---------EKYKGKVSLVVNVASDCQLTDRNYLGLKELHKEFGPSHFS 102
               V+D  GR + L          +  G+V LV NVAS    T R++  + +L ++F    F+
Sbjct: 1233353 VRDDAGRKIPLFRAARAPHRRRSLGQVVLVENVASI*GTTVRDFTQMNDLTEKFA-GKFT 1233529

Query: 103     VLAFPCNQFGESEPRPSKE---VESFARKNYGV--TFPIFHKIKILGSEGEPAFRFLVDS 157
                L FP NQFG+       E   + +  R   G    FPIF K +  G      F+FL  S
Sbjct: 1233530 CLGFPTNQFGKQTNEKDWELLPMLAHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSS 1233709



 Score = 31.6 bits (70), Expect = 0.72
 Identities = 14/35 (40%), Positives = 21/35 (60%)
 Frame = +1

Query: 164     WNFWKYLVNPEGQVVKFWKPEEPIEVIRPDIAALV 198
               WNF K+L+N EG+ VK + P      +  D+ AL+
Sbjct: 1233883 WNFEKFLINQEGKPVKRYSPGFLTADVAADVEALL 1233987


>scaffold_34
          Length = 430012

 Score = 68.6 bits (166), Expect = 5e-12
 Identities = 42/146 (28%), Positives = 69/146 (47%), Gaps = 12/146 (8%)
 Frame = -1

Query: 44     INSFYAFEVKDAKGRTVSLEKYKGKVSLVVNVASDCQLTDRNYLGLKELHKEFGPSHFSV 103
              +  F+    +D  G  V    ++G+V  V N+AS    T+ +Y GL +L  +F     ++
Sbjct: 212230 VGGFHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 104    LAFPCNQFGESEPRPSKEVESFARKNYGV-----------TFPIFHKIKILGSEGEPAFR 152
              + FPCNQF   EP    E+++FAR    +            F +  K  + G+  +P F 
Sbjct: 212050 VGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 153    FLVDSSKKEPRWNFWKY-LVNPEGQV 177
              +L   +  +  WNF  Y LV+ +G V
Sbjct: 211870 YLKREAPCKISWNFGAYFLVSRDGAV 211793


>scaffold_8
          Length = 1961717

 Score = 56.6 bits (135), Expect = 2e-08
 Identities = 38/132 (28%), Positives = 66/132 (50%), Gaps = 8/132 (6%)
 Frame = +3

Query: 56      KGRTVSLEKYKGKVSLVVNVASDCQLTDRNYLGLKELHKEFGPSHFSVLAFPCNQFGESE 115
               KG+   L K++G   LVVN+  D  +   N+  L+ +   +      V AFP  Q G  E
Sbjct: 1062483 KGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYE 1062659

Query: 116     PRPSKEVESFARKNYGV-TFP---IFHKIKILGSEGEPAFRFLVDSSKKEP----RWNFW 167
               P  S+ V + A   +G  T+P   +F KI ++G+   P +R+L  ++          NF 
Sbjct: 1062660 PDVSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLALATNPNGVGRLTVNFE 1062839

Query: 168     KYLVNPEGQVVK 179
               K+L++ +G+ ++
Sbjct: 1062840 KFLLDGDGKPLR 1062875


>scaffold_972
          Length = 3742

 Score = 52.8 bits (125), Expect = 3e-07
 Identities = 49/169 (28%), Positives = 75/169 (44%), Gaps = 5/169 (2%)
 Frame = +3

Query: 14   PRAKVFAVLLSIV-LCTVTXXXXXXXXXXPKINSFYAFEVKDAKGRTVSLEKYKGKVSLV 72
            P A+  A  L++  L T            P   +FY        G  V+  +++GKV   
Sbjct: 3147 PMARRAATTLALATLATAFAPMPATARSAPVRAAFYDLADVANDGSEVAFSQFEGKVCYA 3326

Query: 73   VNVASDCQLTDRNYLGLKELHKEFGPSHFSVLAFPCNQFGESEPRPSKEVESFARKNYGV 132
            VNVAS    T   Y  +K+L  EFG    +V+AFP  +FG  E    + +  FA     V
Sbjct: 3327 VNVASA*GATRPGYKLMKQLSDEFG-DDLAVIAFPTGEFGGQELPTDEAIAKFAYDR--V 3497

Query: 133  TFPIFHKIKIL--GSEGEPAFRFL-VDSSKKEPRWNF-WKYLVNPEGQV 177
             FP   +  +L     G  +++ +  ++    P WNF  K+LV+ +G V
Sbjct: 3498 KFPAAPRGVLLQKADLGRASWQAMQAEAGADAPAWNFKGKFLVSKDGAV 3644


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.138    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,005,879
Number of Sequences: 1185
Number of extensions: 145283
Number of successful extensions: 596
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 40
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 449
Number of HSP's gapped (non-prelim): 256
length of query: 209
length of database: 18,886,859
effective HSP length: 99
effective length of query: 110
effective length of database: 18,769,544
effective search space: 2064649840
effective search space used: 2064649840
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000065_1.0 # Protein # Glutathione peroxidase 1 (GPx1) #
Pan troglodytes # Complete
         (201 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            131   5e-31
scaffold_34                                                            72   4e-13
scaffold_69                                                            68   6e-12
scaffold_8                                                             67   2e-11
scaffold_972                                                           44   2e-04
scaffold_16                                                            29   4.3  
scaffold_58                                                            28   5.6  
scaffold_20                                                            28   5.6  
scaffold_3                                                             28   5.6  
scaffold_41                                                            28   7.4  
scaffold_4                                                             28   9.6  

>scaffold_6
          Length = 2755913

 Score =  131 bits (330), Expect = 5e-31
 Identities = 74/142 (52%), Positives = 90/142 (63%), Gaps = 6/142 (4%)
 Frame = +2

Query: 35      GKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSL 94
               G+V+L+ENVAS+ GTTVRD+TQMN+L  +   +    LGFP NQFG Q N K+ E+L  L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 95      KYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTD----PKLITW 150
                +VRPGGGF PNF +F K E NG GA  LF FLR +L APSDD     +D     K I W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 151     SPVCRND--VAWNFEKFLVGPD 170
               +PV R D      FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 81.3 bits (199), Expect = 7e-16
 Identities = 68/175 (38%), Positives = 82/175 (46%)
 Frame = +3

Query: 21      PLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFG 80
               PL GG  V+L +   +  LI N AS  G T +       LQ + G R L VL FPC+QF 
Sbjct: 2424474 PLGGGGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR-LQVLAFPCDQF- 2424644

Query: 81      HQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATA 140
                QE     EI            F   F LF K +VNG  AHPLFAFL+EA    +     
Sbjct: 2424645 KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPP 2424809

Query: 141     LMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLS 195
                    +  T       DV WNF K+LV  DG P +RYS   +   IE DI A L+
Sbjct: 2424810 WNNKGQAAT-------DVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAALN 2424950



 Score = 79.7 bits (195), Expect = 2e-15
 Identities = 62/160 (38%), Positives = 84/160 (52%), Gaps = 2/160 (1%)
 Frame = +2

Query: 21      PLAGGEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPR--GLVVLGFPCNQ 78
               PL GG P++    R +  L+ N AS  G       Q+ ELQ  L  R  GL VL FPC+Q
Sbjct: 1870712 PLGGGAPLTPARYRRRAHLVVNTASRCGFA----PQLRELQA-LWARYPGLAVLAFPCDQ 1870876

Query: 79      FGHQENAKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDA 138
               FG QE     +I ++  Y R G     +F +FEK +VNG  AHPLF +L++A        
Sbjct: 1870877 FGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNGPRAHPLFTYLKKA-------- 1871014

Query: 139     TALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYS 178
                   T  + +    + R++V WNF K+L+  DG  LRRYS
Sbjct: 1871015 ----TAGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYS 1871119



 Score = 59.7 bits (143), Expect = 2e-09
 Identities = 31/67 (46%), Positives = 40/67 (59%)
 Frame = +1

Query: 132     PAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIE 191
               PA  DD   + T  K  + S     D+AWNFEKFL+  +G P++RYS  F T D+  D+E
Sbjct: 1233802 PASCDDFREIWTG-KRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVE 1233978

Query: 192     ALLSQGP 198
               ALL  GP
Sbjct: 1233979 ALLEHGP 1233999



 Score = 28.5 bits (62), Expect = 5.6
 Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
 Frame = +3

Query: 20     RPLAGGEPVSLGSLR--GKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLG 73
              RP+  G       LR  G+  L ++   L G  +R   +M  L RRLGPRG +  G
Sbjct: 385173 RPVRDGPLALEEELRVVGEERLEQHAERLRGRRIRAGPRMASLGRRLGPRGRLSAG 385340


>scaffold_34
          Length = 430012

 Score = 72.0 bits (175), Expect = 4e-13
 Identities = 55/180 (30%), Positives = 78/180 (43%), Gaps = 10/180 (5%)
 Frame = -1

Query: 25     GEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQEN 84
              G  V   + RG+V+ + N+AS  G T   Y  + +L  +   RGL ++GFPCNQF  QE 
Sbjct: 212191 GAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFPCNQFMGQEP 212012

Query: 85     AKNEEILNSLKYVRPGGGFEP---------NFMLFEKCEVNGAGAHPLFAFLREALPAPS 135
                  EI     + R  G  +P          F L  KC+V+GA   P+F++L+   P   
Sbjct: 212011 GAESEI---KAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKREAP--- 211850

Query: 136    DDATALMTDPKLITWSPVCRNDVAWNF-EKFLVGPDGVPLRRYSRRFQTIDIEPDIEALL 194
                                C+  ++WNF   FLV  DG             D+ P +EALL
Sbjct: 211849 ------------------CK--ISWNFGAYFLVSRDGAV--EAHENAHPRDLAPRVEALL 211736


>scaffold_69
          Length = 78151

 Score = 68.2 bits (165), Expect = 6e-12
 Identities = 41/103 (39%), Positives = 53/103 (51%)
 Frame = +2

Query: 26    EPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENA 85
             EP+ LG        I +  S  G T   Y+QM EL   LG  GL +L FPCNQFG+QE A
Sbjct: 68645 EPLKLG--------ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPA 68800

Query: 86    KNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLR 128
               E +    K       +  +F + EK  V+G  AHP++ FLR
Sbjct: 68801 TCEVVAEFAK-----SAYGADFTMMEKVAVHGPRAHPVYNFLR 68914



 Score = 28.5 bits (62), Expect = 5.6
 Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
 Frame = +3

Query: 23    AGGEPVSLGSLRGKVLLIENVASLXG-TTVRDYTQMNELQRRLGPR 67
             A G  ++  + +GKV+L+ NVAS  G T   + +    LQR    R
Sbjct: 68412 AAGATLTFDAFKGKVVLLTNVASA*GKTAAHEKSDSTSLQRECSAR 68549


>scaffold_8
          Length = 1961717

 Score = 66.6 bits (161), Expect = 2e-11
 Identities = 47/157 (29%), Positives = 72/157 (45%)
 Frame = +3

Query: 25      GEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQEN 84
               G+P  L   RG   L+ N+         ++  +  +      +GL V  FP  Q  ++ +
Sbjct: 1062486 GKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQGYYEPD 1062665

Query: 85      AKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTD 144
                   E++ +  Y + G G  P  ++F+K +V G  AHPL+ +L            AL T+
Sbjct: 1062666 VS--ELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL------------ALATN 1062803

Query: 145     PKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRF 181
               P  +    V       NFEKFL+  DG PLRRY R+F
Sbjct: 1062804 PNGVGRLTV-------NFEKFLLDGDGKPLRRYPRKF 1062893


>scaffold_972
          Length = 3742

 Score = 43.5 bits (101), Expect = 2e-04
 Identities = 42/148 (28%), Positives = 54/148 (36%), Gaps = 1/148 (0%)
 Frame = +3

Query: 25   GEPVSLGSLRGKVLLIENVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQEN 84
            G  V+     GKV    NVAS  G T   Y  M +L    G   L V+ FP  +FG QE 
Sbjct: 3279 GSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIAFPTGEFGGQE- 3452

Query: 85   AKNEEILNSLKYVRPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTD 144
               +E +    Y R      P  +L +K ++  A    + A      P            
Sbjct: 3453 LPTDEAIAKFAYDRVKFPAAPRGVLLQKADLGRASWQAMQAEAGADAP------------ 3596

Query: 145  PKLITWSPVCRNDVAWNFE-KFLVGPDG 171
                          AWNF+ KFLV  DG
Sbjct: 3597 --------------AWNFKGKFLVSKDG 3638


>scaffold_16
          Length = 966663

 Score = 28.9 bits (63), Expect = 4.3
 Identities = 24/87 (27%), Positives = 39/87 (44%), Gaps = 3/87 (3%)
 Frame = +2

Query: 116    NGAGAHPLFAFLREALPAPSDDATALMTDP--KLITWSPVCRNDVAWNFEKFLVGPDGVP 173
              + A +HP  A L  A+ A +  A  + T P  +L +W P+     +W    ++ G + +P
Sbjct: 803633 HSAPSHPSGAALGAAVSAAA--APTVFTTPALQLTSWRPMASKVTSW--RSWIFGTEKMP 803800

Query: 174    LRRYSR-RFQTIDIEPDIEALLSQGPS 199
               +   R    T D EP +    S  PS
Sbjct: 803801 SQSQFRIAVSTTDAEPRVTTTPSSQPS 803881


>scaffold_58
          Length = 213184

 Score = 28.5 bits (62), Expect = 5.6
 Identities = 16/43 (37%), Positives = 19/43 (44%), Gaps = 4/43 (9%)
 Frame = +2

Query: 98     RPGGG----FEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSD 136
              R GGG     EP  +L E C   G G HP  +  R+  P   D
Sbjct: 170612 RQGGGRAGAVEPRLVLRESCRHRGGGRHPAASRCRDWQPRRRD 170740


>scaffold_20
          Length = 721792

 Score = 28.5 bits (62), Expect = 5.6
 Identities = 18/48 (37%), Positives = 19/48 (39%)
 Frame = +1

Query: 98     RPGGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDP 145
              RPG G EP      +    GAGA P     R A P P   A    T P
Sbjct: 298288 RPGAGAEPRLRR-PRARARGAGARPRPPAPRPAAPPPPRGAPRAATPP 298428


>scaffold_3
          Length = 3001400

 Score = 28.5 bits (62), Expect = 5.6
 Identities = 17/62 (27%), Positives = 26/62 (41%)
 Frame = +2

Query: 42      NVASLXGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGG 101
               NV +     V D T    L+  + PRG V+ G   + F  +      E+ N+     PG 
Sbjct: 1322372 NVDATYLLPVDDATVREALEETMAPRGAVIRGAGSSGFLRRSKVSETELRNAADRPVPGA 1322551

Query: 102     GF 103
               G+
Sbjct: 1322552 GW 1322557


>scaffold_41
          Length = 407180

 Score = 28.1 bits (61), Expect = 7.4
 Identities = 14/41 (34%), Positives = 21/41 (51%)
 Frame = +2

Query: 120    AHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAW 160
              A+ L +  R  + A SD   A     +L+ W+PV + D AW
Sbjct: 228698 ANTLISLARAPVAAASDGLAA-----RLLRWAPVVQGDAAW 228805


>scaffold_4
          Length = 3024044

 Score = 27.7 bits (60), Expect = 9.6
 Identities = 17/44 (38%), Positives = 24/44 (54%)
 Frame = -2

Query: 109     LFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSP 152
               LFE+ E +G  A  L   LR++LPA +  A A    P +I+  P
Sbjct: 1000981 LFERQEDSGFCAELLVLDLRKSLPATASPAPAAPPAPAVISSEP 1000850


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.321    0.140    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,894,982
Number of Sequences: 1185
Number of extensions: 290614
Number of successful extensions: 1179
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 599
Number of HSP's gapped (non-prelim): 855
length of query: 201
length of database: 18,886,859
effective HSP length: 99
effective length of query: 102
effective length of database: 18,769,544
effective search space: 1914493488
effective search space used: 1914493488
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000066_1.0 # Protein # Glutathione peroxidase 2 (GPx2) #
Pan troglodytes # Complete
         (190 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            115   4e-26
scaffold_34                                                            75   4e-14
scaffold_8                                                             59   3e-09
scaffold_972                                                           56   3e-08
scaffold_69                                                            49   4e-06
scaffold_23                                                            34   0.093
scaffold_2                                                             31   1.0  
scaffold_19                                                            28   6.6  
scaffold_33                                                            28   8.7  

>scaffold_6
          Length = 2755913

 Score =  115 bits (287), Expect = 4e-26
 Identities = 66/153 (43%), Positives = 87/153 (56%), Gaps = 6/153 (3%)
 Frame = +2

Query: 28      GRAVLIENVASLXGTTTRDFTQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLK 87
               G+ VL+ENVAS+ GTT RDFTQ+N+L  +F  +   LGFP NQFG Q N ++ E+L  L 
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGKFTCLGFPTNQFGKQTNEKDWELLPMLA 1233604

Query: 88      YVRPGGGYQPTFTLVQKCEVNGQNEHPKFAYLKDKLPYPYDD----PFSLMTDPKLIIWS 143
               +VRPGGG+ P F +  K E NG+     F +L+  L  P DD        +   K IIW+
Sbjct: 1233605 HVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIWT 1233784

Query: 144     PVRRSD--VAWNFEKFLIGPEGEPFRRYSRTFP 174
               PV R D      FE+F  G +     R +RT P
Sbjct: 1233785 PVTRRDQRPVTTFERFGPGKDRHRRAR*ARTSP 1233883



 Score = 68.6 bits (166), Expect = 4e-12
 Identities = 59/184 (32%), Positives = 87/184 (47%), Gaps = 1/184 (0%)
 Frame = +2

Query: 6       KSFYDLSAISLDG-EKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRRLVVL 64
               ++++D   + L G   +    +R RA L+ N AS  G   +   +L  L  R+P  L VL
Sbjct: 1870685 RTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ-LRELQALWARYPG-LAVL 1870858

Query: 65      GFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPKFAYLKDKLP 124
                FPC+QFG QE     +I ++  Y R    Y   F + +K +VNG   HP F YLK    
Sbjct: 1870859 AFPCDQFGGQEPGSAGDIRDA--YDR----YGVDFPVFEKSDVNGPRAHPLFTYLKK--- 1871011

Query: 125     YPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKR 184
                         T  + +    + R +V WNF K+LI  +GE  RRYS      ++  D+  
Sbjct: 1871012 ---------ATAGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVVY 1871161

Query: 185     LLKV 188
                LK+
Sbjct: 1871162 TLKL 1871173



 Score = 68.6 bits (166), Expect = 4e-12
 Identities = 68/191 (35%), Positives = 87/191 (45%), Gaps = 6/191 (3%)
 Frame = +3

Query: 2       AFIAKSFYDLSAISLDGE-KVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRR 60
               A  A S+ D +   L G   V  +T+  RA LI N AS  G T +       LQ +F  R
Sbjct: 2424435 AAAAASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR 2424611

Query: 61      LVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPKFAYLK 120
               L VL FPC+QF  QE     EI  +  Y    G    TF L  K +VNG + HP FA+LK
Sbjct: 2424612 LQVLAFPCDQF-KQEPGSPAEI--AAFYADKFG---VTFPLFAKGDVNGPDAHPLFAFLK 2424773

Query: 121     D-----KLPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPT 175
               +       P P   P++               +DV WNF K+L+  +G P +RYS     
Sbjct: 2424774 EATKDQAAPVP---PWNNKGQ---------AATDVMWNFTKWLV-VDGTPTKRYSYDVKP 2424914

Query: 176     INIEPDIKRLL 186
                 IE DI   L
Sbjct: 2424915 EAIEADIAAAL 2424947



 Score = 50.1 bits (118), Expect = 2e-06
 Identities = 25/54 (46%), Positives = 34/54 (62%), Gaps = 7/54 (12%)
 Frame = +1

Query: 141     IWSPVRRS-------DVAWNFEKFLIGPEGEPFRRYSRTFPTINIEPDIKRLLK 187
               IW+  R S       D+AWNFEKFLI  EG+P +RYS  F T ++  D++ LL+
Sbjct: 1233829 IWTGKRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALLE 1233990


>scaffold_34
          Length = 430012

 Score = 75.5 bits (184), Expect = 4e-14
 Identities = 47/127 (37%), Positives = 67/127 (52%), Gaps = 10/127 (7%)
 Frame = -1

Query: 8      FYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFP-RRLVVLGF 66
              F+DL +  +DG +V F+ FRG+ V + N+AS  G T   +  L +L  +F  R L ++GF
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 67     PCNQFGHQENCQNEEILNSLKYVRPGGGYQP---------TFTLVQKCEVNGQNEHPKFA 117
              PCNQF  QE     EI     + R  G   P          F L++KC+V+G    P F+
Sbjct: 212041 PCNQFMGQEPGAESEI---KAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 118    YLKDKLP 124
              YLK + P
Sbjct: 211870 YLKREAP 211850


>scaffold_8
          Length = 1961717

 Score = 59.3 bits (142), Expect = 3e-09
 Identities = 49/171 (28%), Positives = 76/171 (44%), Gaps = 1/171 (0%)
 Frame = +3

Query: 4       IAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRR-LV 62
               +A   +DL  +   G+    + FRG A L+ N+         +F  L  +   +  + L 
Sbjct: 1062447 VATKLFDLQ-VPFKGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLR 1062623

Query: 63      VLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPKFAYLKDK 122
               V  FP  Q  ++ +    E++ +  Y + G G  PT  +  K +V G   HP + YL   
Sbjct: 1062624 VWAFPTEQGYYEPDVS--ELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL--- 1062788

Query: 123     LPYPYDDPFSLMTDPKLIIWSPVRRSDVAWNFEKFLIGPEGEPFRRYSRTF 173
                        +L T+P     + V R  V  NFEKFL+  +G+P RRY R F
Sbjct: 1062789 ---------ALATNP-----NGVGRLTV--NFEKFLLDGDGKPLRRYPRKF 1062893


>scaffold_972
          Length = 3742

 Score = 55.8 bits (133), Expect = 3e-08
 Identities = 33/104 (31%), Positives = 51/104 (49%)
 Frame = +3

Query: 4    IAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPRRLVV 63
            +  +FYDL+ ++ DG +V F+ F G+     NVAS  G T   +  + +L   F   L V
Sbjct: 3237 VRAAFYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGDDLAV 3416

Query: 64   LGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEV 107
            + FP  +FG QE    +E +    Y R      P   L+QK ++
Sbjct: 3417 IAFPTGEFGGQE-LPTDEAIAKFAYDRVKFPAAPRGVLLQKADL 3545


>scaffold_69
          Length = 78151

 Score = 48.9 bits (115), Expect = 4e-06
 Identities = 29/89 (32%), Positives = 43/89 (48%), Gaps = 1/89 (1%)
 Frame = +2

Query: 33    IENVASLXGTTTRDFTQLNELQCRFPRR-LVVLGFPCNQFGHQENCQNEEILNSLKYVRP 91
             I +  S  G T   ++Q+ EL        L +L FPCNQFG+QE    E +    K    
Sbjct: 68663 ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK---- 68830

Query: 92    GGGYQPTFTLVQKCEVNGQNEHPKFAYLK 120
                Y   FT+++K  V+G   HP + +L+
Sbjct: 68831 -SAYGADFTMMEKVAVHGPRAHPVYNFLR 68914



 Score = 40.0 bits (92), Expect = 0.002
 Identities = 19/40 (47%), Positives = 26/40 (65%)
 Frame = +3

Query: 4     IAKSFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTT 43
             +A SFYDL A +  G  + F+ F+G+ VL+ NVAS  G T
Sbjct: 68376 VASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKT 68495


>scaffold_23
          Length = 674037

 Score = 34.3 bits (77), Expect = 0.093
 Identities = 18/38 (47%), Positives = 23/38 (60%)
 Frame = -1

Query: 7      SFYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTT 44
              SF+DL   +  GE VDF T +G+ VL  NVA L   T+
Sbjct: 120669 SFFDLKGRTNAGEAVDFATHKGKCVLAVNVARL*DVTS 120556


>scaffold_2
          Length = 3465789

 Score = 30.8 bits (68), Expect = 1.0
 Identities = 29/115 (25%), Positives = 44/115 (38%), Gaps = 2/115 (1%)
 Frame = -3

Query: 8      FYDLSAISLDGEKVDFNTFRGRAVLIENVASLXGTTTRDFTQLNELQCRFPR--RLVVLG 65
              FY L A +  G    F+    R  +I NV         +   L  +         + +L 
Sbjct: 153661 FYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQILA 153482

Query: 66     FPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFTLVQKCEVNGQNEHPKFAYLK 120
              FP ++FG +   Q          V P      +F L+ K  V+G + HP F YL+
Sbjct: 153481 FPSDEFGPKFTDQEFVDFFVSCGVHPNDA---SFRLMAKVRVDGPDAHPVFKYLR 153326



 Score = 27.7 bits (60), Expect = 8.7
 Identities = 13/37 (35%), Positives = 20/37 (54%)
 Frame = -2

Query: 144     PVRRSDVAWNFEKFLIGPEGEPFRRYSRTFPTINIEP 180
               P   +D+A  + +FL    G+PF  YSR F + +  P
Sbjct: 1754522 PALTADMAGLYGRFLNWTRGKPFGSYSRAFGSASRRP 1754412


>scaffold_19
          Length = 762245

 Score = 28.1 bits (61), Expect = 6.6
 Identities = 15/29 (51%), Positives = 17/29 (58%)
 Frame = -3

Query: 143    SPVRRSDVAWNFEKFLIGPEGEPFRRYSR 171
              +P RRSDVAW F +F     GE F   SR
Sbjct: 357858 TPRRRSDVAWVFRRF----SGESFADISR 357784


>scaffold_33
          Length = 466611

 Score = 27.7 bits (60), Expect = 8.7
 Identities = 16/69 (23%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
 Frame = -3

Query: 87     KYVRPGGGYQPTFTLVQKC-EVNGQNEHPKFAYLKDKLPYPYDDPFSLMTDPKLIIWSPV 145
              + + P  G  P+F+L     E N  +   + +    + P P+  P S++    L+I +  
Sbjct: 268561 RQLMPSDGITPSFSLTALLIETNMLSNLERHSRRHQETPIPHATPKSMLNPANLVITTQA 268382

Query: 146    RRSDVAWNF 154
               R   AW +
Sbjct: 268381 TRLTKAWTW 268355


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.323    0.141    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,886,380
Number of Sequences: 1185
Number of extensions: 205463
Number of successful extensions: 734
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 44
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 541
Number of HSP's gapped (non-prelim): 372
length of query: 190
length of database: 18,886,859
effective HSP length: 98
effective length of query: 92
effective length of database: 18,770,729
effective search space: 1726907068
effective search space used: 1726907068
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000067_1.0 # Protein # Glutathione peroxidase 3 (GPx3) #
Pan troglodytes # Complete
         (226 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             89   4e-18
scaffold_34                                                            61   1e-09
scaffold_69                                                            49   7e-06
scaffold_8                                                             48   8e-06
scaffold_972                                                           41   0.001
scaffold_5                                                             29   5.3  
scaffold_31                                                            28   9.1  

>scaffold_6
          Length = 2755913

 Score = 89.0 bits (219), Expect = 4e-18
 Identities = 57/142 (40%), Positives = 79/142 (55%), Gaps = 11/142 (7%)
 Frame = +2

Query: 61      GKYVLFVNVASYXGLTGQ-YIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTL 119
               G+ VL  NVAS  G T + + ++N L E+ A      LGFP NQFGKQ   ++ E+LP L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 120     KYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSC-PPTSELLG-------TSDRLFW 171
                +VRPGGGFVPNF +F K + NGE     + FL++S   P+ +  G       ++  + W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 172     EPMKVHDIR--WNFEKFLVGPD 191
                P+   D R    FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 70.9 bits (172), Expect = 1e-12
 Identities = 57/172 (33%), Positives = 76/172 (44%), Gaps = 2/172 (1%)
 Frame = +3

Query: 42      EYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQYIELNALQEELAPFG--LVILGF 99
               ++    + G   +    YA +  L  N AS  G+T Q     ALQ   A FG  L +L F
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQ---AKFGDRLQVLAF 2424629

Query: 100     PCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPT 159
               PC+QF KQEPG  +EI            F   F LF KGDVNG      + FLK +   T
Sbjct: 2424630 PCDQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEA---T 2424782

Query: 160     SELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDI 211
                +          +     D+ WNF K+LV  DG P  R+ +      ++ DI
Sbjct: 2424783 KDQAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADI 2424935



 Score = 70.5 bits (171), Expect = 2e-12
 Identities = 52/174 (29%), Positives = 83/174 (47%)
 Frame = +2

Query: 39      TIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQYIELNALQEELAPFGLVILG 98
               T +++  + + G   +   +Y  +  L VN AS  G   Q  EL AL       GL +L 
Sbjct: 1870688 TYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLA 1870861

Query: 99      FPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPP 158
               FPC+QFG QEPG   +I     Y R G     +F +FEK DVNG +    +T+LK +   
Sbjct: 1870862 FPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNGPRAHPLFTYLKKAT-- 1871017

Query: 159     TSELLGTSDRLFWEPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDIL 212
                         +  + +   +++WNF K+L+  DG  + R+    +  +V  D++
Sbjct: 1871018 ------AGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVV 1871158



 Score = 34.7 bits (78), Expect = 0.097
 Identities = 13/34 (38%), Positives = 21/34 (61%)
 Frame = +1

Query: 178     DIRWNFEKFLVGPDGIPIMRWHHRTTVSNVKMDI 211
               D+ WNFEKFL+  +G P+ R+      ++V  D+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADV 1233975


>scaffold_34
          Length = 430012

 Score = 61.2 bits (147), Expect = 1e-09
 Identities = 53/163 (32%), Positives = 72/163 (44%), Gaps = 8/163 (4%)
 Frame = -1

Query: 38     GTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQYIE-LNALQEELAPFGLVI 96
              G  ++  +  IDG E + F  + G+ V   N+AS  G T  + + L  L  + +  GL I
Sbjct: 212227 GGFHDLMSEDIDGAE-VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 97     LGFPCNQFGKQEPGENSEILPTLK---YVRPGGG---FVPNFQLFEKGDVNGEKEQKFYT 150
              +GFPCNQF  QEPG  SEI    +   ++ P  G    V  F L  K DV+G      ++
Sbjct: 212050 VGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 151    FLKNSCPPTSELLGTSDRLFWEPMKVHDIRWNF-EKFLVGPDG 192
              +LK   P                     I WNF   FLV  DG
Sbjct: 211870 YLKREAP-------------------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 48.5 bits (114), Expect = 7e-06
 Identities = 27/75 (36%), Positives = 37/75 (49%)
 Frame = +2

Query: 79    YIELNALQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKG 138
             Y ++  L +EL   GL IL FPCNQFG QEP     +    K       +  +F + EK 
Sbjct: 68705 YSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMMEKV 68869

Query: 139   DVNGEKEQKFYTFLK 153
              V+G +    Y FL+
Sbjct: 68870 AVHGPRAHPVYNFLR 68914


>scaffold_8
          Length = 1961717

 Score = 48.1 bits (113), Expect = 8e-06
 Identities = 45/152 (29%), Positives = 65/152 (42%), Gaps = 3/152 (1%)
 Frame = +3

Query: 55      PFKQYAGKYVLFVNVASYXGLTGQYIELNALQEELAPF---GLVILGFPCNQFGKQEPGE 111
               P  ++ G   L VN+     + G+    NAL+   A +   GL +  FP  Q G  EP +
Sbjct: 1062498 PLSKFRGSAHLVVNIKMDDPVAGE--NFNALRYVAANYAEKGLRVWAFPTEQ-GYYEP-D 1062665

Query: 112     NSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTSELLGTSDRLFW 171
                SE++    Y + G G  P   +F+K DV G      Y +L                L  
Sbjct: 1062666 VSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLA---------------LAT 1062800

Query: 172     EPMKVHDIRWNFEKFLVGPDGIPIMRWHHRTT 203
                P  V  +  NFEKFL+  DG P+ R+  + T
Sbjct: 1062801 NPNGVGRLTVNFEKFLLDGDGKPLRRYPRKFT 1062896


>scaffold_972
          Length = 3742

 Score = 41.2 bits (95), Expect = 0.001
 Identities = 49/166 (29%), Positives = 64/166 (38%), Gaps = 9/166 (5%)
 Frame = +3

Query: 36   ISGTIYEYGALTIDGEEYIPFKQYAGKYVLFVNVASYXGLTGQ-YIELNALQEELAPFGL 94
            +    Y+   +  DG E + F Q+ GK    VNVAS  G T   Y  +  L +E     L
Sbjct: 3237 VRAAFYDLADVANDGSE-VAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DL 3410

Query: 95   VILGFPCNQFGKQEPGENSEILPTLKYVRPGGGFVPNFQLFEKGDVNGEKEQKF-YTFLK 153
             ++ FP  +FG QE       LPT                        E   KF Y  +K
Sbjct: 3411 AVIAFPTGEFGGQE-------LPT-----------------------DEAIAKFAYDRVK 3500

Query: 154  NSCPPTSELLGTSD--RLFWEPMKVH----DIRWNFE-KFLVGPDG 192
                P   LL  +D  R  W+ M+         WNF+ KFLV  DG
Sbjct: 3501 FPAAPRGVLLQKADLGRASWQAMQAEAGADAPAWNFKGKFLVSKDG 3638


>scaffold_5
          Length = 3000003

 Score = 28.9 bits (63), Expect = 5.3
 Identities = 16/42 (38%), Positives = 24/42 (57%)
 Frame = -2

Query: 85     LQEELAPFGLVILGFPCNQFGKQEPGENSEILPTLKYVRPGG 126
              +++ELAP  L+ L  P ++   Q PG +    P L+  RPGG
Sbjct: 557438 VRQELAPGVLLHLRTPHHRRRVQRPGGHEAQAPLLQGARPGG 557313


>scaffold_31
          Length = 470201

 Score = 28.1 bits (61), Expect = 9.1
 Identities = 12/26 (46%), Positives = 17/26 (65%), Gaps = 2/26 (7%)
 Frame = -3

Query: 30     MDCHGGISGTIYEYGALT--IDGEEY 53
              M C+GGI  ++Y Y AL+  +DG  Y
Sbjct: 156882 MGCNGGIPSSVYSYWALSGIVDGGNY 156805


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.140    0.434 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,391,052
Number of Sequences: 1185
Number of extensions: 175039
Number of successful extensions: 576
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 45
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 423
Number of HSP's gapped (non-prelim): 256
length of query: 226
length of database: 18,886,859
effective HSP length: 100
effective length of query: 126
effective length of database: 18,768,359
effective search space: 2364813234
effective search space used: 2364813234
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000068_1.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Pan troglodytes # Complete
         (197 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             88   6e-18
scaffold_34                                                            87   1e-17
scaffold_69                                                            84   8e-17
scaffold_8                                                             67   1e-11
scaffold_972                                                           57   2e-08
scaffold_2                                                             40   0.002
scaffold_39                                                            28   7.1  

>scaffold_6
          Length = 2755913

 Score = 88.2 bits (217), Expect = 6e-18
 Identities = 57/141 (40%), Positives = 80/141 (56%), Gaps = 5/141 (3%)
 Frame = +2

Query: 58      KYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIK 117
               +YR    +V N AS+ G       +L  L ARY   GL +LAFPC+QFG QEPGS  +I+
Sbjct: 1870745 RYRRRAHLVVNTASRCGFAP-QLRELQALWARYP--GLAVLAFPCDQFGGQEPGSAGDIR 1870915

Query: 118     EFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKG-----KGILGNAIKWNFTKFLIDK 172
               +    Y V F +F K  VNG  AHPL+ ++K    G     KG+  + ++WNFTK+LI  
Sbjct: 1870916 DAYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVPNKGLPRDEVQWNFTKWLI-V 1871092

Query: 173     NGCVVKRYGPMEEPLVIEKDL 193
               +G V++RY     P  +  D+
Sbjct: 1871093 DGEVLRRYSFDASPKSVLADV 1871155



 Score = 84.7 bits (208), Expect = 6e-17
 Identities = 65/170 (38%), Positives = 87/170 (51%), Gaps = 14/170 (8%)
 Frame = +3

Query: 38      ARSMHEFSAKDIDGH-MVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECG-- 94
               A S  +F+   + G   V L  Y     ++ N AS+ G T     QL    A  A+ G  
Sbjct: 2424444 AASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTP----QLGGFQALQAKFGDR 2424611

Query: 95      LRILAFPCNQFGKQEPGSNEEIKEFAAG-YNVKFDMFSKICVNGDDAHPLWKWMKIQPK- 152
               L++LAFPC+QF KQEPGS  EI  F A  + V F +F+K  VNG DAHPL+ ++K   K 
Sbjct: 2424612 LQVLAFPCDQF-KQEPGSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKD 2424788

Query: 153     ---------GKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193
                         KG     + WNFTK+L+  +G   KRY    +P  IE D+
Sbjct: 2424789 QAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADI 2424935



 Score = 49.7 bits (117), Expect = 2e-06
 Identities = 34/94 (36%), Positives = 50/94 (53%), Gaps = 6/94 (6%)
 Frame = +2

Query: 61      GFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA 120
               G V +V NVAS  G T  ++TQ+ DL  ++A      L FP NQFGKQ    + E+    
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 121     A------GYNVKFDMFSKICVNGDDAHPLWKWMK 148
               A      G+   F +F+K   NG+ A  L+K+++
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLR 1233703



 Score = 38.5 bits (88), Expect = 0.005
 Identities = 18/45 (40%), Positives = 24/45 (53%)
 Frame = +1

Query: 138     DDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGP 182
               DD   +W   +  P     +G  + WNF KFLI++ G  VKRY P
Sbjct: 1233814 DDFREIWTGKRSSPSRS--VGTDLAWNFEKFLINQEGKPVKRYSP 1233942


>scaffold_34
          Length = 430012

 Score = 87.4 bits (215), Expect = 1e-17
 Identities = 55/148 (37%), Positives = 78/148 (52%), Gaps = 13/148 (8%)
 Frame = -1

Query: 42     HEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFP 101
              H+  ++DIDG  V    +RG V  VTN+AS+ GKT  +Y  L  L A+++  GL I+ FP
Sbjct: 212218 HDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFP 212039

Query: 102    CNQFGKQEPGSNEEIKEFA------------AGYNVKFDMFSKICVNGDDAHPLWKWMKI 149
              CNQF  QEPG+  EIK FA            A     F++  K  V+G    P++ ++K 
Sbjct: 212038 CNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKR 211859

Query: 150    QPKGKGILGNAIKWNF-TKFLIDKNGCV 176
              +   K      I WNF   FL+ ++G V
Sbjct: 211858 EAPCK------ISWNFGAYFLVSRDGAV 211793


>scaffold_69
          Length = 78151

 Score = 84.3 bits (207), Expect = 8e-17
 Identities = 44/116 (37%), Positives = 63/116 (54%), Gaps = 1/116 (0%)
 Frame = +2

Query: 66    VTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFA-AGYN 124
             +++  S  GKT  +Y+Q+V+LH      GL ILAFPCNQFG QEP + E + EFA + Y 
Sbjct: 68663 ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYG 68842

Query: 125   VKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRY 180
               F M  K+ V+G  AHP++ ++      +   G    WNF  + +      VK Y
Sbjct: 68843 ADFTMMEKVAVHGPRAHPVYNFL------RATTGATPSWNFGVYFVVSRSGEVKAY 68992



 Score = 34.3 bits (77), Expect = 0.099
 Identities = 24/73 (32%), Positives = 34/73 (46%)
 Frame = +3

Query: 38    ARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRI 97
             A S ++  AK   G  +  D ++G V ++TNVAS  GKT  +  +  D  +   EC  R 
Sbjct: 68379 ASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAH--EKSDSTSLQRECSAR- 68549

Query: 98    LAFPCNQFGKQEP 110
                    F KQ P
Sbjct: 68550 ------AFRKQHP 68570


>scaffold_8
          Length = 1961717

 Score = 67.4 bits (163), Expect = 1e-11
 Identities = 44/130 (33%), Positives = 67/130 (51%), Gaps = 5/130 (3%)
 Frame = +3

Query: 56      LDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEE 115
               L K+RG   +V N+         N+  L  + A YAE GLR+ AFP  Q G  EP  +E 
Sbjct: 1062501 LSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYEPDVSEL 1062677

Query: 116     IK-----EFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLI 170
               ++     +F  G      +F KI V G+ AHPL++++ +     G+    +  NF KFL+
Sbjct: 1062678 VRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLALATNPNGV--GRLTVNFEKFLL 1062851

Query: 171     DKNGCVVKRY 180
               D +G  ++RY
Sbjct: 1062852 DGDGKPLRRY 1062881


>scaffold_972
          Length = 3742

 Score = 56.6 bits (135), Expect = 2e-08
 Identities = 43/135 (31%), Positives = 62/135 (45%), Gaps = 3/135 (2%)
 Frame = +3

Query: 50   DGHMVNLDKYRGFVCIVTNVASQXGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQE 109
            DG  V   ++ G VC   NVAS  G T   Y  +  L   + +  L ++AFP  +FG QE
Sbjct: 3276 DGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIAFPTGEFGGQE 3452

Query: 110  PGSNEEIKEFAAGYNVKFDMFSK--ICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFT- 166
              ++E I +FA    VKF    +  +    D     W+ M+ +        +A  WNF  
Sbjct: 3453 LPTDEAIAKFAYD-RVKFPAAPRGVLLQKADLGRASWQAMQAEAG-----ADAPAWNFKG 3614

Query: 167  KFLIDKNGCVVKRYG 181
            KFL+ K+G V    G
Sbjct: 3615 KFLVSKDGAVTDASG 3659


>scaffold_2
          Length = 3465789

 Score = 40.0 bits (92), Expect = 0.002
 Identities = 20/61 (32%), Positives = 39/61 (63%), Gaps = 5/61 (8%)
 Frame = -3

Query: 94     GLRILAFPCNQFGKQEPGSNEEIKEFAAGYNV-----KFDMFSKICVNGDDAHPLWKWMK 148
              G++ILAFP ++FG +   +++E  +F     V      F + +K+ V+G DAHP++K+++
Sbjct: 153499 GVQILAFPSDEFGPK--FTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLR 153326

Query: 149    I 149
              +
Sbjct: 153325 L 153323


>scaffold_39
          Length = 370504

 Score = 28.1 bits (61), Expect = 7.1
 Identities = 12/39 (30%), Positives = 23/39 (58%), Gaps = 2/39 (5%)
 Frame = -3

Query: 129   MFSKICVNGDDAHPLWKWMKIQPKGKG--ILGNAIKWNF 165
             +F+   +  ++ H LW+W+++ P  +G  IL  A +W F
Sbjct: 66197 IFTLCSIIVNNFHKLWRWLELFPNARGGFIL*RARRWVF 66081


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.324    0.140    0.457 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,739,614
Number of Sequences: 1185
Number of extensions: 161450
Number of successful extensions: 586
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 471
Number of HSP's gapped (non-prelim): 263
length of query: 197
length of database: 18,886,859
effective HSP length: 99
effective length of query: 98
effective length of database: 18,769,544
effective search space: 1839415312
effective search space used: 1839415312
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000069_1.0 # Protein # Glutathione peroxidase 5 (GPx5) #
Pan troglodytes # Complete
         (220 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             89   4e-18
scaffold_34                                                            54   1e-07
scaffold_69                                                            45   7e-05
scaffold_8                                                             44   1e-04
scaffold_972                                                           37   0.019
scaffold_22                                                            28   8.7  

>scaffold_6
          Length = 2755913

 Score = 89.0 bits (219), Expect = 4e-18
 Identities = 57/146 (39%), Positives = 80/146 (54%), Gaps = 12/146 (8%)
 Frame = +2

Query: 57      KQYVGKHILFVNVATYCGLTAQ-YPELNAPEELKPYGLVVLGFPCNQFGKQEPGDNKEIL 115
               ++ +G+ +L  NVA+  G T + + ++N   E        LGFP NQFGKQ    + E+L
Sbjct: 1233413 RRSLGQVVLVENVASI*GTTVRDFTQMNDLTEKFAGKFTCLGFPTNQFGKQTNEKDWELL 1233592

Query: 116     PALKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSE---------ILGTFK 166
               P L +VRPGGGFVP+F +F K + NGE    +F FL+ S   PS+         ++ T K
Sbjct: 1233593 PMLAHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVIST-K 1233769

Query: 167     SISWDPVKVHDIR--WNFEKFLVGPD 190
               +I W PV   D R    FE+F  G D
Sbjct: 1233770 NIIWTPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 88.6 bits (218), Expect = 5e-18
 Identities = 70/196 (35%), Positives = 99/196 (50%), Gaps = 4/196 (2%)
 Frame = +2

Query: 24      KQEKMKMDCHKDEK-GTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYPEL 82
               K E    D  K E+  T +D+E + L     ++  +Y  +  L VN A+ CG   Q  EL
Sbjct: 1870640 KLEAAAHDARKQERLRTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLREL 1870819

Query: 83      NAPEELKPYGLVVLGFPCNQFGKQEPGDNKEILPALKYVRPGGGFVPSFQLFEKGDVNGE 142
                A     P GL VL FPC+QFG QEPG   +I  A  Y R G      F +FEK DVNG 
Sbjct: 1870820 QALWARYP-GLAVLAFPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNGP 1870978

Query: 143     KEQKVFSFLKHSC---PHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSH 199
               +   +F++LK +    P P+      K +  D     +++WNF K+L+  DG  + R+S 
Sbjct: 1870979 RAHPLFTYLKKATAGQPVPN------KGLPRD-----EVQWNFTKWLI-VDGEVLRRYSF 1871122

Query: 200     RATVSSVKTDILAYLK 215
                A+  SV  D++  LK
Sbjct: 1871123 DASPKSVLADVVYTLK 1871170



 Score = 78.2 bits (191), Expect = 7e-15
 Identities = 59/173 (34%), Positives = 81/173 (46%)
 Frame = +3

Query: 42      DYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQYPELNAPEELKPYGLVVLGFPCN 101
               D+    L     V+   Y  +  L  N A+ CG+T Q     A +      L VL FPC+
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQAKFGDRLQVLAFPCD 2424638

Query: 102     QFGKQEPGDNKEILPALKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEI 161
               QF KQEPG   EI  A  Y      F  +F LF KGDVNG     +F+FLK +    +  
Sbjct: 2424639 QF-KQEPGSPAEI--AAFY---ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAP 2424800

Query: 162     LGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYL 214
               +  + +         D+ WNF K+LV  DG P  R+S+     +++ DI A L
Sbjct: 2424801 VPPWNN---KGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAAL 2424947



 Score = 38.9 bits (89), Expect = 0.005
 Identities = 17/39 (43%), Positives = 24/39 (61%)
 Frame = +1

Query: 177     DIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAYLK 215
               D+ WNFEKFL+  +G PV R+S     + V  D+ A L+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALLE 1233990


>scaffold_34
          Length = 430012

 Score = 54.3 bits (129), Expect = 1e-07
 Identities = 48/147 (32%), Positives = 63/147 (42%), Gaps = 9/147 (6%)
 Frame = -1

Query: 54     VSFKQYVGKHILFVNVATYCGLT-AQYPEL-NAPEELKPYGLVVLGFPCNQFGKQEPGDN 111
              V F  + G+ +   N+A+  G T + Y  L     +    GL ++GFPCNQF  QEPG  
Sbjct: 212182 VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFPCNQFMGQEPGAE 212003

Query: 112    KEILPALK---YVRPGGG---FVPSFQLFEKGDVNGEKEQKVFSFLKHSCPHPSEILGTF 165
               EI    +   ++ P  G    V +F L  K DV+G     VFS+LK   P         
Sbjct: 212002 SEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKREAP--------- 211850

Query: 166    KSISWDPVKVHDIRWNF-EKFLVGPDG 191
                          I WNF   FLV  DG
Sbjct: 211849 ----------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 45.1 bits (105), Expect = 7e-05
 Identities = 24/67 (35%), Positives = 35/67 (52%)
 Frame = +2

Query: 86    EELKPYGLVVLGFPCNQFGKQEPGDNKEILPALKYVRPGGGFVPSFQLFEKGDVNGEKEQ 145
             +EL   GL +L FPCNQFG QEP   + +    K       +   F + EK  V+G +  
Sbjct: 68729 DELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMMEKVAVHGPRAH 68893

Query: 146   KVFSFLK 152
              V++FL+
Sbjct: 68894 PVYNFLR 68914


>scaffold_8
          Length = 1961717

 Score = 44.3 bits (103), Expect = 1e-04
 Identities = 35/111 (31%), Positives = 53/111 (47%)
 Frame = +3

Query: 92      GLVVLGFPCNQFGKQEPGDNKEILPALKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFL 151
               GL V  FP  Q G  EP D  E++ A  Y + G G  P+  +F+K DV G     ++ +L
Sbjct: 1062615 GLRVWAFPTEQ-GYYEP-DVSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL 1062788

Query: 152     KHSCPHPSEILGTFKSISWDPVKVHDIRWNFEKFLVGPDGIPVMRWSHRAT 202
                              +++ +P  V  +  NFEKFL+  DG P+ R+  + T
Sbjct: 1062789 ---------------ALATNPNGVGRLTVNFEKFLLDGDGKPLRRYPRKFT 1062896



 Score = 29.6 bits (65), Expect = 3.0
 Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 5/64 (7%)
 Frame = +2

Query: 157     HPSEILGTFKSISWDPVK-----VHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDIL 211
               H   + G+ K  SW  +K     V    W F++ L        +RW H     S K+D+L
Sbjct: 1315280 HADTLAGSSKKTSWAGLKGPLVAVAGPTWTFKERLAD------LRWDHGPLPRSAKSDVL 1315441

Query: 212     AYLK 215
               A L+
Sbjct: 1315442 AALE 1315453


>scaffold_972
          Length = 3742

 Score = 37.0 bits (84), Expect = 0.019
 Identities = 44/158 (27%), Positives = 59/158 (37%), Gaps = 3/158 (1%)
 Frame = +3

Query: 37   KGTIYDYEAIALNKNEYVSFKQYVGKHILFVNVATYCGLTAQ-YPELNAPEELKPYGLVV 95
            +   YD   +A N    V+F Q+ GK    VNVA+  G T   Y  +    +     L V
Sbjct: 3240 RAAFYDLADVA-NDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGDDLAV 3416

Query: 96   LGFPCNQFGKQE-PGDNKEILPALKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHS 154
            + FP  +FG QE P D  E +    Y R      P   L +K D+     Q + +     
Sbjct: 3417 IAFPTGEFGGQELPTD--EAIAKFAYDRVKFPAAPRGVLLQKADLGRASWQAMQAEAGAD 3590

Query: 155  CPHPSEILGTFKSISWDPVKVHDIRWNFE-KFLVGPDG 191
             P                       WNF+ KFLV  DG
Sbjct: 3591 AP----------------------AWNFKGKFLVSKDG 3638


>scaffold_22
          Length = 725242

 Score = 28.1 bits (61), Expect = 8.7
 Identities = 25/102 (24%), Positives = 43/102 (42%), Gaps = 4/102 (3%)
 Frame = -3

Query: 116    PALKYVRPGGGFVPSFQLFEKGDVNGEKEQKVFSFLKHSCP----HPSEILGTFKSISWD 171
              PA  ++R GGG VP F++   G   G         +  + P    H  E  G  + I WD
Sbjct: 526406 PAQGHMRGGGGEVPKFRVSAAGGGRGAAGPTSPDHIGGN*PALTAHTGECPGKRR*I-WD 526230

Query: 172    PVKVHDIRWNFEKFLVGPDGIPVMRWSHRATVSSVKTDILAY 213
              P +    R +++   +G +   ++  ++ A      T I+ Y
Sbjct: 526229 PYRARTAR-SWKLLEIGKNSSILLAANYTALQQKAATSIILY 526107


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.138    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,431,753
Number of Sequences: 1185
Number of extensions: 178563
Number of successful extensions: 666
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 58
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 473
Number of HSP's gapped (non-prelim): 371
length of query: 220
length of database: 18,886,859
effective HSP length: 100
effective length of query: 120
effective length of database: 18,768,359
effective search space: 2252203080
effective search space used: 2252203080
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000070_1.0 # Protein # Glutathione peroxidase 6 (GPx6) #
Pan troglodytes # Complete
         (221 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             75   8e-14
scaffold_34                                                            59   5e-09
scaffold_69                                                            55   9e-08
scaffold_8                                                             45   9e-05
scaffold_972                                                           38   0.008
scaffold_13                                                            32   0.46 
scaffold_2                                                             28   6.7  

>scaffold_6
          Length = 2755913

 Score = 74.7 bits (182), Expect = 8e-14
 Identities = 55/170 (32%), Positives = 79/170 (46%)
 Frame = +3

Query: 42      EYGALTLNGEEYIQFKQFAGKHVLFVNVATYXGLAAQYPELNALQEELKNFGVIVLAFPC 101
               ++    L G   +    +A +  L  N A+  G+  Q     ALQ +  +  + VLAFPC
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQAKFGD-RLQVLAFPC 2424635

Query: 102     NQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPPTSD 161
               +QF KQEPG+ +EI            F  +F LF KGDVNG     +F FLK +   T D
Sbjct: 2424636 DQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEA---TKD 2424788

Query: 162     LLGSSSQLFWEPMKVHDICWNFEKFLVGPDGVPVMRWFHQAPVSTVKSDI 211
                         +     D+ WNF K+LV  DG P  R+ +      +++DI
Sbjct: 2424789 QAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADI 2424935



 Score = 73.6 bits (179), Expect = 2e-13
 Identities = 54/178 (30%), Positives = 87/178 (48%)
 Frame = +2

Query: 39      TIYEYGALTLNGEEYIQFKQFAGKHVLFVNVATYXGLAAQYPELNALQEELKNFGVIVLA 98
               T +++  + L G   +   ++  +  L VN A+  G A Q  EL AL       G+ VLA
Sbjct: 1870688 TYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLA 1870861

Query: 99      FPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPP 158
               FPC+QFG QEPG+  +I            +   F +FEK DVNG +   +FT+LK +   
Sbjct: 1870862 FPCDQFGGQEPGSAGDIRDAY------DRYGVDFPVFEKSDVNGPRAHPLFTYLKKAT-- 1871017

Query: 159     TSDLLGSSSQLFWEPMKVHDICWNFEKFLVGPDGVPVMRWFHQAPVSTVKSDILEYLK 216
                     +   +  + +   ++ WNF K+L+  DG  + R+   A   +V +D++  LK
Sbjct: 1871018 ------AGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVVYTLK 1871170



 Score = 71.6 bits (174), Expect = 7e-13
 Identities = 51/142 (35%), Positives = 72/142 (50%), Gaps = 11/142 (7%)
 Frame = +2

Query: 61      GKHVLFVNVATYXGLAAQ-YPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGL 119
               G+ VL  NVA+  G   + + ++N L E+        L FP NQFGKQ    + E+L  L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 120     KYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSC-PPTSDLLG-------SSSQLFW 171
                +V PG GFVP+F +F K + NGE    +F FL++S   P+ D  G       S+  + W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 172     EPMKVHD--ICWNFEKFLVGPD 191
                P+   D      FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 34.7 bits (78), Expect = 0.093
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +1

Query: 178     DICWNFEKFLVGPDGVPVMRWFHQAPVSTVKSDI 211
               D+ WNFEKFL+  +G PV R+      + V +D+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADV 1233975


>scaffold_34
          Length = 430012

 Score = 58.9 bits (141), Expect = 5e-09
 Identities = 50/163 (30%), Positives = 72/163 (44%), Gaps = 8/163 (4%)
 Frame = -1

Query: 38     GTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVATYXGLA-AQYPELNALQEELKNFGVIV 96
              G  ++  +  ++G E ++F  F G+ V   N+A+  G   + Y  L  L  +    G+ +
Sbjct: 212227 GGFHDLMSEDIDGAE-VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 97     LAFPCNQFGKQEPGTNSEILLGLK---YVCPGSG---FVPSFQLFEKGDVNGEKEQKVFT 150
              + FPCNQF  QEPG  SEI    +   ++ P  G    V +F L  K DV+G     VF+
Sbjct: 212050 VGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 151    FLKNSCPPTSDLLGSSSQLFWEPMKVHDICWNF-EKFLVGPDG 192
              +LK   P                     I WNF   FLV  DG
Sbjct: 211870 YLKREAP-------------------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 54.7 bits (130), Expect = 9e-08
 Identities = 30/78 (38%), Positives = 43/78 (55%)
 Frame = +2

Query: 76    AAQYPELNALQEELKNFGVIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLF 135
             AA Y ++  L +EL + G+ +LAFPCNQFG QEP T   +    K     S +   F + 
Sbjct: 68696 AAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMM 68860

Query: 136   EKGDVNGEKEQKVFTFLK 153
             EK  V+G +   V+ FL+
Sbjct: 68861 EKVAVHGPRAHPVYNFLR 68914


>scaffold_8
          Length = 1961717

 Score = 44.7 bits (104), Expect = 9e-05
 Identities = 44/144 (30%), Positives = 63/144 (43%), Gaps = 3/144 (2%)
 Frame = +3

Query: 58      QFAGKHVLFVNVATYXGLAAQYPELNALQEELKNF---GVIVLAFPCNQFGKQEPGTNSE 114
               +F G   L VN+     +A +    NAL+    N+   G+ V AFP  Q G  EP   SE
Sbjct: 1062507 KFRGSAHLVVNIKMDDPVAGE--NFNALRYVAANYAEKGLRVWAFPTEQ-GYYEPDV-SE 1062674

Query: 115     ILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPM 174
               ++    Y   G G  P+  +F+K DV G     ++ +L                L   P 
Sbjct: 1062675 LVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLA---------------LATNPN 1062809

Query: 175     KVHDICWNFEKFLVGPDGVPVMRW 198
                V  +  NFEKFL+  DG P+ R+
Sbjct: 1062810 GVGRLTVNFEKFLLDGDGKPLRRY 1062881


>scaffold_972
          Length = 3742

 Score = 38.1 bits (87), Expect = 0.008
 Identities = 47/164 (28%), Positives = 65/164 (39%), Gaps = 7/164 (4%)
 Frame = +3

Query: 36   VTGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVATYXGLAAQYPELNALQEELKNFG-- 93
            V    Y+   +  +G E + F QF GK    VNVA+  G  A  P    +++    FG  
Sbjct: 3237 VRAAFYDLADVANDGSE-VAFSQFEGKVCYAVNVASA*G--ATRPGYKLMKQLSDEFGDD 3407

Query: 94   VIVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLK 153
            + V+AFP  +FG QE  T+ E +    Y        P   L +K D              
Sbjct: 3408 LAVIAFPTGEFGGQELPTD-EAIAKFAYDRVKFPAAPRGVLLQKAD-------------- 3542

Query: 154  NSCPPTSDLLGSSSQLFWEPMKVH----DICWNFE-KFLVGPDG 192
                     LG +S   W+ M+         WNF+ KFLV  DG
Sbjct: 3543 ---------LGRAS---WQAMQAEAGADAPAWNFKGKFLVSKDG 3638


>scaffold_13
          Length = 1338096

 Score = 32.3 bits (72), Expect = 0.46
 Identities = 26/94 (27%), Positives = 38/94 (40%), Gaps = 23/94 (24%)
 Frame = -3

Query: 138    GDVNGEKEQKVFTFLKNSCPPTSDLLGSSSQLFWEPM---------------KVHDICWN 182
              G  + E EQ+V+  +  SCP  +  + S+   F  PM               K+ D+  +
Sbjct: 701473 GAQSTESEQRVYVAIVCSCPRNASDVYSTKSRFSSPMKGAYWTLTWWSTPGLKIDDLTRS 701294

Query: 183    FEKFLVGPDGVP--------VMRWFHQAPVSTVK 208
              F  F+    GVP        + RWF  A   TVK
Sbjct: 701293 FAAFVRACVGVPSARFRMSLLPRWFDDATTFTVK 701192


>scaffold_2
          Length = 3465789

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 30/119 (25%), Positives = 49/119 (41%), Gaps = 2/119 (1%)
 Frame = -3

Query: 37     TGTIYEYGALTLNGEEYIQFKQFAGKHVLFVNVATYXGLAAQ-YPELNALQEELKNF-GV 94
              T   Y+  A T  G  +  F     +  + VNV  +   A +    L  + +++    GV
Sbjct: 153670 TSRFYKLVAHTARGWPF-PFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGV 153494

Query: 95     IVLAFPCNQFGKQEPGTNSEILLGLKYVCPGSGFVPSFQLFEKGDVNGEKEQKVFTFLK 153
               +LAFP ++FG   P    +  +     C       SF+L  K  V+G     VF +L+
Sbjct: 153493 QILAFPSDEFG---PKFTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLR 153326


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.322    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,178,004
Number of Sequences: 1185
Number of extensions: 180220
Number of successful extensions: 482
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 27
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 388
Number of HSP's gapped (non-prelim): 201
length of query: 221
length of database: 18,886,859
effective HSP length: 100
effective length of query: 121
effective length of database: 18,768,359
effective search space: 2270971439
effective search space used: 2270971439
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000071_1.0 # Protein # Glutathione peroxidase 7 (GPx7) #
Pan troglodytes # Complete
         (134 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             74   4e-14
scaffold_69                                                            72   2e-13
scaffold_34                                                            71   3e-13
scaffold_8                                                             57   6e-09
scaffold_972                                                           50   6e-07
scaffold_2                                                             45   2e-05

>scaffold_6
          Length = 2755913

 Score = 74.3 bits (181), Expect = 4e-14
 Identities = 51/112 (45%), Positives = 62/112 (55%), Gaps = 2/112 (1%)
 Frame = +2

Query: 25      FYDFKAVNIRGKL-VSLEKYRGSVSLVVNVASECGFTDQHYRALQQL-QRDLGPHHFNVL 82
               ++DF+ V + G   ++  +YR    LVVN AS CGF  Q  R LQ L  R  G     VL
Sbjct: 1870691 YWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ-LRELQALWARYPG---LAVL 1870858

Query: 83      AFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLAR 134
               AFPC+QFG QEP S  +I     R Y V FP+F K  V G  AHP F YL +
Sbjct: 1870859 AFPCDQFGGQEPGSAGDIRDAYDR-YGVDFPVFEKSDVNGPRAHPLFTYLKK 1871011



 Score = 72.0 bits (175), Expect = 2e-13
 Identities = 43/98 (43%), Positives = 54/98 (55%)
 Frame = +3

Query: 35      GKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEP 94
               G  V+L  Y     L+ N AS+CG T Q     Q LQ   G     VLAFPC+QF +QEP
Sbjct: 2424486 GGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR-LQVLAFPCDQF-KQEP 2424656

Query: 95      DSNKEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYL 132
                S  EI +F    + V+FP+F+K  V G  AHP F +L
Sbjct: 2424657 GSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFL 2424770



 Score = 45.8 bits (107), Expect = 1e-05
 Identities = 31/93 (33%), Positives = 43/93 (46%), Gaps = 5/93 (5%)
 Frame = +2

Query: 45      GSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFA 104
               G V LV NVAS  G T + +  +  L        F  L FP NQFG+Q  + + E+    
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 105     RRT-----YSVSFPMFSKIAVTGTGAHPAFKYL 132
                       +  +FP+F+K    G GA   FK+L
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFL 1233700


>scaffold_69
          Length = 78151

 Score = 71.6 bits (174), Expect = 2e-13
 Identities = 32/75 (42%), Positives = 43/75 (57%)
 Frame = +2

Query: 58    GFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESFARRTYSVSFPMFSK 117
             G T  HY  + +L  +LG     +LAFPCNQFG QEP + + +  FA+  Y   F M  K
Sbjct: 68687 GKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEK 68866

Query: 118   IAVTGTGAHPAFKYL 132
             +AV G  AHP + +L
Sbjct: 68867 VAVHGPRAHPVYNFL 68911



 Score = 35.0 bits (79), Expect = 0.025
 Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
 Frame = +3

Query: 25    FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRA-LQQLQRDLGPHHF 79
             FYD KA    G  ++ + ++G V L+ NVAS  G T  H ++    LQR+     F
Sbjct: 68388 FYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAHEKSDSTSLQRECSARAF 68555


>scaffold_34
          Length = 430012

 Score = 71.2 bits (173), Expect = 3e-13
 Identities = 41/121 (33%), Positives = 58/121 (47%), Gaps = 11/121 (9%)
 Frame = -1

Query: 25     FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAF 84
              F+D  + +I G  V    +RG V  V N+AS  G T+ HY+ L +L          ++ F
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 85     PCNQFGQQEPDSNKEIESFARRTYSV-----------SFPMFSKIAVTGTGAHPAFKYLA 133
              PCNQF  QEP +  EI++FAR    +           +F +  K  V+G    P F YL 
Sbjct: 212041 PCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLK 211862

Query: 134    R 134
              R
Sbjct: 211861 R 211859


>scaffold_8
          Length = 1961717

 Score = 57.0 bits (136), Expect = 6e-09
 Identities = 38/108 (35%), Positives = 53/108 (49%), Gaps = 5/108 (4%)
 Frame = +3

Query: 31      VNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFG 90
               V  +GK   L K+RGS  LVVN+  +     +++ AL+ +  +       V AFP  Q G
Sbjct: 1062474 VPFKGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-G 1062650

Query: 91      QQEPDSN-----KEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYLA 133
                 EPD +     K    F   TY  +  +F KI V G  AHP ++YLA
Sbjct: 1062651 YYEPDVSELVRAKAYSQFGFGTYPTAV-VFDKIDVIGNTAHPLYRYLA 1062791


>scaffold_972
          Length = 3742

 Score = 50.4 bits (119), Expect = 6e-07
 Identities = 30/80 (37%), Positives = 42/80 (52%)
 Frame = +3

Query: 25   FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAF 84
            FYD   V   G  V+  ++ G V   VNVAS  G T   Y+ ++QL  + G     V+AF
Sbjct: 3249 FYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFG-DDLAVIAF 3425

Query: 85   PCNQFGQQEPDSNKEIESFA 104
            P  +FG QE  +++ I  FA
Sbjct: 3426 PTGEFGGQELPTDEAIAKFA 3485


>scaffold_2
          Length = 3465789

 Score = 45.4 bits (106), Expect = 2e-05
 Identities = 32/111 (28%), Positives = 47/111 (42%), Gaps = 3/111 (2%)
 Frame = -3

Query: 25     FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGP-HHFNVLA 83
              FY   A   RG     +       ++VNV        +   AL+++  D+ P     +LA
Sbjct: 153661 FYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQILA 153482

Query: 84     FPCNQFGQQEPDSN--KEIESFARRTYSVSFPMFSKIAVTGTGAHPAFKYL 132
              FP ++FG +  D        S        SF + +K+ V G  AHP FKYL
Sbjct: 153481 FPSDEFGPKFTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYL 153329


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.322    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,296,944
Number of Sequences: 1185
Number of extensions: 87165
Number of successful extensions: 284
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 224
Number of HSP's gapped (non-prelim): 112
length of query: 134
length of database: 18,886,859
effective HSP length: 92
effective length of query: 42
effective length of database: 18,777,839
effective search space: 788669238
effective search space used: 788669238
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000098_1.0 # Protein # Glutathione peroxidase 1 (GPx1) #
Mus musculus # Complete
         (201 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            131   6e-31
scaffold_34                                                            73   3e-13
scaffold_69                                                            69   4e-12
scaffold_8                                                             66   3e-11
scaffold_972                                                           40   0.002
scaffold_4                                                             33   0.18 
scaffold_9                                                             30   1.9  
scaffold_14                                                            28   5.6  
scaffold_10                                                            28   9.6  

>scaffold_6
          Length = 2755913

 Score =  131 bits (329), Expect = 6e-31
 Identities = 73/142 (51%), Positives = 92/142 (64%), Gaps = 6/142 (4%)
 Frame = +2

Query: 35      GKVLLIENVASLXGTTIRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQENGKNEEILNSL 94
               G+V+L+ENVAS+ GTT+RD+T+MNDL ++   +    LGFP NQFG Q N K+ E+L  L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 95      KYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPTPSDDPTALMTD----PKYIIW 150
                +VRPGGGF PNF +F K E NGE A  LF FLR++L  PSDD     +D     K IIW
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 151     SPVCRND--IAWNFEKFLVGPD 170
               +PV R D      FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 83.6 bits (205), Expect = 1e-16
 Identities = 67/187 (35%), Positives = 93/187 (49%), Gaps = 11/187 (5%)
 Frame = +2

Query: 3       AARLSAAAQS--------TVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLXGTTIRDY 54
               A +L AAA          T + F   PL GG P++    R +  L+ N AS  G   +  
Sbjct: 1870634 AYKLEAAAHDARKQERLRTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ-L 1870810

Query: 55      TEMNDLQKRLGPRGLVVLGFPCNQFGHQENGKNEEILNSLKYVRPGGGFEPNFTLFEKCE 114
                E+  L  R    GL VL FPC+QFG QE G   +I ++  Y R G     +F +FEK +
Sbjct: 1870811 RELQALWARYP--GLAVLAFPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSD 1870966

Query: 115     VNGEKAHPLFTFLRNAL---PTPSDDPTALMTDPKYIIWSPVCRNDIAWNFEKFLVGPDG 171
               VNG +AHPLFT+L+ A    P P+                 + R+++ WNF K+L+  DG
Sbjct: 1870967 VNGPRAHPLFTYLKKATAGQPVPN---------------KGLPRDEVQWNFTKWLI-VDG 1871098

Query: 172     VPVRRYS 178
                 +RRYS
Sbjct: 1871099 EVLRRYS 1871119



 Score = 82.8 bits (203), Expect = 3e-16
 Identities = 72/194 (37%), Positives = 91/194 (46%), Gaps = 2/194 (1%)
 Frame = +3

Query: 4       ARLSAAAQSTVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLXGTTIRDYTEMNDLQKR 63
               A   AAA ++   F+  PL GG  V+L +   +  LI N AS  G T +       LQ +
Sbjct: 2424423 AAAPAAAAASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAK 2424599

Query: 64      LGPRGLVVLGFPCNQFGHQENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPL 123
                G R L VL FPC+QF  QE G   EI            F   F LF K +VNG  AHPL
Sbjct: 2424600 FGDR-LQVLAFPCDQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPL 2424758

Query: 124     FTFLRNALPTPSDDPTALMTDPKYIIWS--PVCRNDIAWNFEKFLVGPDGVPVRRYSRRF 181
               F FL+ A    + D  A +       W+       D+ WNF K+LV  DG P +RYS   
Sbjct: 2424759 FAFLKEA----TKDQAAPVPP-----WNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDV 2424908

Query: 182     RTIDIEPDIETLLS 195
               +   IE DI   L+
Sbjct: 2424909 KPEAIEADIAAALN 2424950



 Score = 52.8 bits (125), Expect = 3e-07
 Identities = 22/38 (57%), Positives = 28/38 (73%)
 Frame = +1

Query: 157     DIAWNFEKFLVGPDGVPVRRYSRRFRTIDIEPDIETLL 194
               D+AWNFEKFL+  +G PV+RYS  F T D+  D+E LL
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALL 1233987


>scaffold_34
          Length = 430012

 Score = 72.8 bits (177), Expect = 3e-13
 Identities = 56/180 (31%), Positives = 78/180 (43%), Gaps = 10/180 (5%)
 Frame = -1

Query: 25     GEPVSLGSLRGKVLLIENVASLXGTTIRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQEN 84
              G  V   + RG+V+ + N+AS  G T   Y  +  L  +   RGL ++GFPCNQF  QE 
Sbjct: 212191 GAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFPCNQFMGQEP 212012

Query: 85     GKNEEILNSLKYVRPGGGFEP---------NFTLFEKCEVNGEKAHPLFTFLRNALPTPS 135
              G   EI     + R  G  +P          F L  KC+V+G    P+F++L+   P   
Sbjct: 212011 GAESEI---KAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKREAP--- 211850

Query: 136    DDPTALMTDPKYIIWSPVCRNDIAWNF-EKFLVGPDGVPVRRYSRRFRTIDIEPDIETLL 194
                                C+  I+WNF   FLV  DG      +   R  D+ P +E LL
Sbjct: 211849 ------------------CK--ISWNFGAYFLVSRDGAVEAHENAHPR--DLAPRVEALL 211736


>scaffold_69
          Length = 78151

 Score = 68.9 bits (167), Expect = 4e-12
 Identities = 39/103 (37%), Positives = 54/103 (52%)
 Frame = +2

Query: 26    EPVSLGSLRGKVLLIENVASLXGTTIRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQENG 85
             EP+ LG        I +  S  G T   Y++M +L   LG  GL +L FPCNQFG+QE  
Sbjct: 68645 EPLKLG--------ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPA 68800

Query: 86    KNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLR 128
               E +    K       +  +FT+ EK  V+G +AHP++ FLR
Sbjct: 68801 TCEVVAEFAK-----SAYGADFTMMEKVAVHGPRAHPVYNFLR 68914



 Score = 27.7 bits (60), Expect = 9.6
 Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
 Frame = +3

Query: 2     CAARLSAAAQSTVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLXG-TTIRDYTEMNDL 60
             CAA   A   S+ Y   A+   G   ++  + +GKV+L+ NVAS  G T   + ++   L
Sbjct: 68361 CAAHHVA---SSFYDLKAKTAAGAT-LTFDAFKGKVVLLTNVASA*GKTAAHEKSDSTSL 68528

Query: 61    QKRLGPR 67
             Q+    R
Sbjct: 68529 QRECSAR 68549


>scaffold_8
          Length = 1961717

 Score = 65.9 bits (159), Expect = 3e-11
 Identities = 47/157 (29%), Positives = 72/157 (45%)
 Frame = +3

Query: 25      GEPVSLGSLRGKVLLIENVASLXGTTIRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQEN 84
               G+P  L   RG   L+ N+         ++  +  +      +GL V  FP  Q G+ E 
Sbjct: 1062486 GKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYEP 1062662

Query: 85      GKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPTPSDDPTALMTD 144
                 +E ++ +  Y + G G  P   +F+K +V G  AHPL+ +L            AL T+
Sbjct: 1062663 DVSE-LVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL------------ALATN 1062803

Query: 145     PKYIIWSPVCRNDIAWNFEKFLVGPDGVPVRRYSRRF 181
               P  +         +  NFEKFL+  DG P+RRY R+F
Sbjct: 1062804 PNGV-------GRLTVNFEKFLLDGDGKPLRRYPRKF 1062893



 Score = 27.7 bits (60), Expect = 9.6
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = -1

Query: 3      AARLSAAAQSTVYAFSARPLTGGEPVSLGSLRGK 36
              A+R SAA ++T  A ++RP  GG P    + RG+
Sbjct: 759815 ASRASAAPRATWPARASRPAAGGGPAPTTTRRGR 759714


>scaffold_972
          Length = 3742

 Score = 39.7 bits (91), Expect = 0.002
 Identities = 35/115 (30%), Positives = 45/115 (39%)
 Frame = +3

Query: 1    MCAARLSAAAQSTVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLXGTTIRDYTEMNDL 60
            M A   SA  ++  Y   A     G  V+     GKV    NVAS  G T   Y  M  L
Sbjct: 3210 MPATARSAPVRAAFYDL-ADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQL 3386

Query: 61   QKRLGPRGLVVLGFPCNQFGHQENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEV 115
                G   L V+ FP  +FG QE    +E +    Y R      P   L +K ++
Sbjct: 3387 SDEFGD-DLAVIAFPTGEFGGQEL-PTDEAIAKFAYDRVKFPAAPRGVLLQKADL 3545


>scaffold_4
          Length = 3024044

 Score = 33.5 bits (75), Expect = 0.18
 Identities = 22/70 (31%), Positives = 34/70 (48%)
 Frame = +1

Query: 119     KAHPLFTFLRNALPTPSDDPTALMTDPKYIIWSPVCRNDIAWNFEKFLVGPDGVPVRRYS 178
               +AH L   LR+  P P   P A +   + ++     R  +A   ++ ++G D V VRR  
Sbjct: 2597227 RAHELHQVLRHHEPQPRGGPRAAVRRVRRLVRVGPRRRLVA---QREVLGEDAVLVRRVD 2597397

Query: 179     RRFRTIDIEP 188
                R R  D+EP
Sbjct: 2597398 ARARVADLEP 2597427


>scaffold_9
          Length = 1602328

 Score = 30.0 bits (66), Expect = 1.9
 Identities = 22/63 (34%), Positives = 32/63 (50%)
 Frame = -2

Query: 7      SAAAQSTVYAFSARPLTGGEPVSLGSLRGKVLLIENVASLXGTTIRDYTEMNDLQKRLGP 66
              SAA  + V A  + P  G   + LGS  G      +VA+L   T  D+ E++ ++K LG 
Sbjct: 125187 SAAVSAAVVALLSAPAAGPGALRLGSSAG-----TSVATL---TYGDFLELDGIKKFLGD 125032

Query: 67     RGL 69
               GL
Sbjct: 125031 EGL 125023


>scaffold_14
          Length = 1107847

 Score = 28.5 bits (62), Expect = 5.6
 Identities = 17/41 (41%), Positives = 20/41 (48%)
 Frame = -2

Query: 103    FEPNFTLFEKCEVNGEKAHPLFTFLRNALPTPSDDPTALMT 143
              F P+  L    +      HP  T LR + PTPS  PTAL T
Sbjct: 801642 FSPHLPLLLFFQHQSHNQHPQCTTLRTS-PTPSLSPTALKT 801523


>scaffold_10
          Length = 1654512

 Score = 27.7 bits (60), Expect = 9.6
 Identities = 13/28 (46%), Positives = 13/28 (46%)
 Frame = +1

Query: 128     RNALPTPSDDPTALMTDPKYIIWSPVCR 155
               R A PT S    A  T PK   W P CR
Sbjct: 1312453 RMAPPTASPTENAAATPPKTSAWPPTCR 1312536


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.319    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,106,633
Number of Sequences: 1185
Number of extensions: 289233
Number of successful extensions: 1348
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 94
Number of HSP's successfully gapped in prelim test: 19
Number of HSP's that attempted gapping in prelim test: 708
Number of HSP's gapped (non-prelim): 989
length of query: 201
length of database: 18,886,859
effective HSP length: 99
effective length of query: 102
effective length of database: 18,769,544
effective search space: 1914493488
effective search space used: 1914493488
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000099_1.0 # Protein # Glutathione peroxidase 2 (GPx2) #
Mus musculus # Complete
         (190 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            109   2e-24
scaffold_34                                                            80   1e-15
scaffold_8                                                             62   4e-10
scaffold_972                                                           58   8e-09
scaffold_69                                                            50   2e-06
scaffold_23                                                            33   0.27 
scaffold_2                                                             30   1.3  

>scaffold_6
          Length = 2755913

 Score =  109 bits (273), Expect = 2e-24
 Identities = 62/147 (42%), Positives = 85/147 (57%), Gaps = 6/147 (4%)
 Frame = +2

Query: 28      GRAVLIENVASLXGTTTRDYNQLNELQCRFPRRLVVLGFPCNQFGHQENCQNEEILNSLK 87
               G+ VL+ENVAS+ GTT RD+ Q+N+L  +F  +   LGFP NQFG Q N ++ E+L  L 
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGKFTCLGFPTNQFGKQTNEKDWELLPMLA 1233604

Query: 88      YVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLKDKLPYPYDD----PFSLMTDPKLIIWS 143
               +VRPGGG+ P F +  K + NG+    +F +L+  L  P DD        +   K IIW+
Sbjct: 1233605 HVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIWT 1233784

Query: 144     PVRRSDVS--WNFEKFLIGPEGEPFRR 168
               PV R D      FE+F  GP  +  RR
Sbjct: 1233785 PVTRRDQRPVTTFERF--GPGKDRHRR 1233859



 Score = 72.4 bits (176), Expect = 3e-13
 Identities = 61/184 (33%), Positives = 88/184 (47%), Gaps = 1/184 (0%)
 Frame = +2

Query: 6       KSFYDLSAVGLDG-EKIDFNTFRGRAVLIENVASLXGTTTRDYNQLNELQCRFPRRLVVL 64
               ++++D   V L G   +    +R RA L+ N AS  G   +   +L  L  R+P  L VL
Sbjct: 1870685 RTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ-LRELQALWARYPG-LAVL 1870858

Query: 65      GFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLKDKLP 124
                FPC+QFG QE     +I ++  Y R    Y   F + +K DVNG   HP+F YLK    
Sbjct: 1870859 AFPCDQFGGQEPGSAGDIRDA--YDR----YGVDFPVFEKSDVNGPRAHPLFTYLKK--- 1871011

Query: 125     YPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRSFQTINIEPDIKR 184
                         T  + +    + R +V WNF K+LI  +GE  RRYS      ++  D+  
Sbjct: 1871012 ---------ATAGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVVY 1871161

Query: 185     LLKV 188
                LK+
Sbjct: 1871162 TLKL 1871173



 Score = 72.0 bits (175), Expect = 4e-13
 Identities = 68/191 (35%), Positives = 89/191 (46%), Gaps = 6/191 (3%)
 Frame = +3

Query: 2       AYIAKSFYDLSAVGLDGE-KIDFNTFRGRAVLIENVASLXGTTTRDYNQLNELQCRFPRR 60
               A  A S+ D +   L G   +  +T+  RA LI N AS  G T +       LQ +F  R
Sbjct: 2424435 AAAAASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR 2424611

Query: 61      LVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLK 120
               L VL FPC+QF  QE     EI  +  Y    G    TF L  K DVNG + HP+FA+LK
Sbjct: 2424612 LQVLAFPCDQF-KQEPGSPAEI--AAFYADKFG---VTFPLFAKGDVNGPDAHPLFAFLK 2424773

Query: 121     D-----KLPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRSFQT 175
               +       P P   P++               +DV WNF K+L+  +G P +RYS   + 
Sbjct: 2424774 EATKDQAAPVP---PWNNKGQ---------AATDVMWNFTKWLV-VDGTPTKRYSYDVKP 2424914

Query: 176     INIEPDIKRLL 186
                 IE DI   L
Sbjct: 2424915 EAIEADIAAAL 2424947



 Score = 50.1 bits (118), Expect = 2e-06
 Identities = 24/54 (44%), Positives = 34/54 (62%), Gaps = 7/54 (12%)
 Frame = +1

Query: 141     IWSPVRRS-------DVSWNFEKFLIGPEGEPFRRYSRSFQTINIEPDIKRLLK 187
               IW+  R S       D++WNFEKFLI  EG+P +RYS  F T ++  D++ LL+
Sbjct: 1233829 IWTGKRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALLE 1233990


>scaffold_34
          Length = 430012

 Score = 80.5 bits (197), Expect = 1e-15
 Identities = 49/127 (38%), Positives = 68/127 (53%), Gaps = 10/127 (7%)
 Frame = -1

Query: 8      FYDLSAVGLDGEKIDFNTFRGRAVLIENVASLXGTTTRDYNQLNELQCRFP-RRLVVLGF 66
              F+DL +  +DG ++ F+ FRG+ V + N+AS  G T   Y  L +L  +F  R L ++GF
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 67     PCNQFGHQENCQNEEILNSLKYVRPGGGYQP---------TFSLTQKCDVNGQNEHPVFA 117
              PCNQF  QE     EI     + R  G   P          F+L +KCDV+G    PVF+
Sbjct: 212041 PCNQFMGQEPGAESEI---KAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 118    YLKDKLP 124
              YLK + P
Sbjct: 211870 YLKREAP 211850


>scaffold_8
          Length = 1961717

 Score = 62.0 bits (149), Expect = 4e-10
 Identities = 48/171 (28%), Positives = 77/171 (45%), Gaps = 1/171 (0%)
 Frame = +3

Query: 4       IAKSFYDLSAVGLDGEKIDFNTFRGRAVLIENVASLXGTTTRDYNQLNELQCRFPRR-LV 62
               +A   +DL  V   G+    + FRG A L+ N+         ++N L  +   +  + L 
Sbjct: 1062447 VATKLFDLQ-VPFKGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLR 1062623

Query: 63      VLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFAYLKDK 122
               V  FP  Q  ++ +    E++ +  Y + G G  PT  +  K DV G   HP++ YL   
Sbjct: 1062624 VWAFPTEQGYYEPDVS--ELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL--- 1062788

Query: 123     LPYPYDDPFSLMTDPKLIIWSPVRRSDVSWNFEKFLIGPEGEPFRRYSRSF 173
                        +L T+P  +         ++ NFEKFL+  +G+P RRY R F
Sbjct: 1062789 ---------ALATNPNGV-------GRLTVNFEKFLLDGDGKPLRRYPRKF 1062893


>scaffold_972
          Length = 3742

 Score = 57.8 bits (138), Expect = 8e-09
 Identities = 36/106 (33%), Positives = 50/106 (47%)
 Frame = +3

Query: 2    AYIAKSFYDLSAVGLDGEKIDFNTFRGRAVLIENVASLXGTTTRDYNQLNELQCRFPRRL 61
            A +  +FYDL+ V  DG ++ F+ F G+     NVAS  G T   Y  + +L   F   L
Sbjct: 3231 APVRAAFYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGDDL 3410

Query: 62   VVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDV 107
             V+ FP  +FG QE    +E +    Y R      P   L QK D+
Sbjct: 3411 AVIAFPTGEFGGQE-LPTDEAIAKFAYDRVKFPAAPRGVLLQKADL 3545


>scaffold_69
          Length = 78151

 Score = 50.1 bits (118), Expect = 2e-06
 Identities = 30/89 (33%), Positives = 43/89 (48%), Gaps = 1/89 (1%)
 Frame = +2

Query: 33    IENVASLXGTTTRDYNQLNELQCRFPRR-LVVLGFPCNQFGHQENCQNEEILNSLKYVRP 91
             I +  S  G T   Y+Q+ EL        L +L FPCNQFG+QE    E +    K    
Sbjct: 68663 ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK---- 68830

Query: 92    GGGYQPTFSLTQKCDVNGQNEHPVFAYLK 120
                Y   F++ +K  V+G   HPV+ +L+
Sbjct: 68831 -SAYGADFTMMEKVAVHGPRAHPVYNFLR 68914



 Score = 40.4 bits (93), Expect = 0.001
 Identities = 19/41 (46%), Positives = 26/41 (63%)
 Frame = +3

Query: 3     YIAKSFYDLSAVGLDGEKIDFNTFRGRAVLIENVASLXGTT 43
             ++A SFYDL A    G  + F+ F+G+ VL+ NVAS  G T
Sbjct: 68373 HVASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKT 68495


>scaffold_23
          Length = 674037

 Score = 32.7 bits (73), Expect = 0.27
 Identities = 17/38 (44%), Positives = 22/38 (57%)
 Frame = -1

Query: 7      SFYDLSAVGLDGEKIDFNTFRGRAVLIENVASLXGTTT 44
              SF+DL      GE +DF T +G+ VL  NVA L   T+
Sbjct: 120669 SFFDLKGRTNAGEAVDFATHKGKCVLAVNVARL*DVTS 120556


>scaffold_2
          Length = 3465789

 Score = 30.4 bits (67), Expect = 1.3
 Identities = 33/123 (26%), Positives = 47/123 (38%), Gaps = 4/123 (3%)
 Frame = -3

Query: 2      AYIAKS--FYDLSAVGLDGEKIDFNTFRGRAVLIENVASLXGTTTRDYNQLNELQCRFPR 59
              AY  K+  FY L A    G    F+    R  +I NV         +   L  +      
Sbjct: 153685 AYHTKTSRFYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISP 153506

Query: 60     --RLVVLGFPCNQFGHQENCQNEEILNSLKYVRPGGGYQPTFSLTQKCDVNGQNEHPVFA 117
                 + +L FP ++FG +   Q          V P      +F L  K  V+G + HPVF 
Sbjct: 153505 VDGVQILAFPSDEFGPKFTDQEFVDFFVSCGVHPNDA---SFRLMAKVRVDGPDAHPVFK 153335

Query: 118    YLK 120
              YL+
Sbjct: 153334 YLR 153326


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.322    0.141    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,090,654
Number of Sequences: 1185
Number of extensions: 210032
Number of successful extensions: 558
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 43
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 401
Number of HSP's gapped (non-prelim): 290
length of query: 190
length of database: 18,886,859
effective HSP length: 98
effective length of query: 92
effective length of database: 18,770,729
effective search space: 1726907068
effective search space used: 1726907068
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000100_1.0 # Protein # Glutathione peroxidase 3 (GPx3) #
Mus musculus # Complete
         (223 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             83   2e-16
scaffold_34                                                            54   1e-07
scaffold_69                                                            47   2e-05
scaffold_8                                                             46   3e-05
scaffold_972                                                           42   8e-04
scaffold_2                                                             29   4.0  
scaffold_13                                                            28   8.9  

>scaffold_6
          Length = 2755913

 Score = 83.2 bits (204), Expect = 2e-16
 Identities = 56/143 (39%), Positives = 77/143 (53%), Gaps = 15/143 (10%)
 Frame = +2

Query: 61      GKYILFVNVASYGD----QYLELNALQEEL-GPFGLVILGFPSNQFGKQEPGENSEILPS 115
               G+ +L  NVAS        + ++N L E+  G F    LGFP+NQFGKQ   ++ E+LP 
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGKF--TCLGFPTNQFGKQTNEKDWELLPM 1233598

Query: 116     LKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSC-PPTAELLG-------SPGRLF 167
               L +VRPGGGFVPNF +F K + NGE     + FL++S   P+ +  G       S   + 
Sbjct: 1233599 LAHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNII 1233778

Query: 168     WEPMKIHDIR--WNFEKFLVGPD 188
               W P+   D R    FE+F  G D
Sbjct: 1233779 WTPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 62.4 bits (150), Expect = 4e-10
 Identities = 52/174 (29%), Positives = 85/174 (48%), Gaps = 3/174 (1%)
 Frame = +2

Query: 39      TIYEYGALTIDGEEYIPFKQYAGKYILFVNVAS---YGDQYLELNALQEELGPFGLVILG 95
               T +++  + + G   +   +Y  +  L VN AS   +  Q  EL AL       GL +L 
Sbjct: 1870688 TYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLA 1870861

Query: 96      FPSNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPP 155
               FP +QFG QEPG   +I  +  Y R G     +F +FEK DVNG +    +T+LK +   
Sbjct: 1870862 FPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNGPRAHPLFTYLKKA--- 1871014

Query: 156     TAELLGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPVMRWYHRTTVSNVKMDIL 209
                    G P  +  + +   +++WNF K+L+  DG  + R+    +  +V  D++
Sbjct: 1871015 ---TAGQP--VPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVV 1871158



 Score = 61.2 bits (147), Expect = 9e-10
 Identities = 51/169 (30%), Positives = 70/169 (41%), Gaps = 2/169 (1%)
 Frame = +3

Query: 42      EYGALTIDGEEYIPFKQYAGKYILFVNVASYGDQYLELNALQEELGPFG--LVILGFPSN 99
               ++    + G   +    YA +  L  N AS      +L   Q     FG  L +L FP +
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQAKFGDRLQVLAFPCD 2424638

Query: 100     QFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTAEL 159
               QF KQEPG  +EI            F   F LF KGDVNG      + FLK +    A  
Sbjct: 2424639 QF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAP 2424800

Query: 160     LGSPGRLFWEPMKIHDIRWNFEKFLVGPDGIPVMRWYHRTTVSNVKMDI 208
               +        +     D+ WNF K+LV  DG P  R+ +      ++ DI
Sbjct: 2424801 VPPWNN---KGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADI 2424935



 Score = 34.7 bits (78), Expect = 0.095
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +1

Query: 175     DIRWNFEKFLVGPDGIPVMRWYHRTTVSNVKMDI 208
               D+ WNFEKFL+  +G PV R+      ++V  D+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADV 1233975


>scaffold_34
          Length = 430012

 Score = 54.3 bits (129), Expect = 1e-07
 Identities = 51/163 (31%), Positives = 67/163 (41%), Gaps = 11/163 (6%)
 Frame = -1

Query: 38     GTIYEYGALTIDGEEYIPFKQYAGKYILFVNVASY----GDQYLELNALQEELGPFGLVI 93
              G  ++  +  IDG E + F  + G+ +   N+AS        Y  L  L  +    GL I
Sbjct: 212227 GGFHDLMSEDIDGAE-VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 94     LGFPSNQFGKQEPGENSEI---LPSLKYVRPGGG---FVPNFQLFEKGDVNGEKEQKFYT 147
              +GFP NQF  QEPG  SEI     S  ++ P  G    V  F L  K DV+G      ++
Sbjct: 212050 VGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 148    FLKNSCPPTAELLGSPGRLFWEPMKIHDIRWNF-EKFLVGPDG 189
              +LK   P                     I WNF   FLV  DG
Sbjct: 211870 YLKREAP-------------------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 47.0 bits (110), Expect = 2e-05
 Identities = 27/75 (36%), Positives = 37/75 (49%)
 Frame = +2

Query: 76    YLELNALQEELGPFGLVILGFPSNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKG 135
             Y ++  L +ELG  GL IL FP NQFG QEP     +    K       +  +F + EK 
Sbjct: 68705 YSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMMEKV 68869

Query: 136   DVNGEKEQKFYTFLK 150
              V+G +    Y FL+
Sbjct: 68870 AVHGPRAHPVYNFLR 68914


>scaffold_8
          Length = 1961717

 Score = 46.2 bits (108), Expect = 3e-05
 Identities = 43/150 (28%), Positives = 64/150 (42%), Gaps = 4/150 (2%)
 Frame = +3

Query: 55      PFKQYAGKYILFVNVAS----YGDQYLELNALQEELGPFGLVILGFPSNQFGKQEPGENS 110
               P  ++ G   L VN+       G+ +  L  +       GL +  FP+ Q G  EP + S
Sbjct: 1062498 PLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYEP-DVS 1062671

Query: 111     EILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTAELLGSPGRLFWEP 170
               E++ +  Y + G G  P   +F+K DV G      Y +L  +  P        GRL    
Sbjct: 1062672 ELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLALATNPNGV-----GRL---- 1062824

Query: 171     MKIHDIRWNFEKFLVGPDGIPVMRWYHRTT 200
                       NFEKFL+  DG P+ R+  + T
Sbjct: 1062825 ------TVNFEKFLLDGDGKPLRRYPRKFT 1062896


>scaffold_972
          Length = 3742

 Score = 41.6 bits (96), Expect = 8e-04
 Identities = 44/158 (27%), Positives = 60/158 (37%), Gaps = 9/158 (5%)
 Frame = +3

Query: 41   YEYGALTIDGEEYIPFKQYAGKYILFVNVASY----GDQYLELNALQEELGPFGLVILGF 96
            Y+   +  DG E + F Q+ GK    VNVAS        Y  +  L +E G   L ++ F
Sbjct: 3252 YDLADVANDGSE-VAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIAF 3425

Query: 97   PSNQFGKQEPGENSEILPSLKYVRPGGGFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPT 156
            P+ +FG QE     E +    Y R      P   L +K D+                   
Sbjct: 3426 PTGEFGGQEL-PTDEAIAKFAYDRVKFPAAPRGVLLQKADL------------------- 3545

Query: 157  AELLGSPGRLFWEPMKIH----DIRWNFE-KFLVGPDG 189
                   GR  W+ M+         WNF+ KFLV  DG
Sbjct: 3546 -------GRASWQAMQAEAGADAPAWNFKGKFLVSKDG 3638


>scaffold_2
          Length = 3465789

 Score = 29.3 bits (64), Expect = 4.0
 Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 5/53 (9%)
 Frame = -3

Query: 55     PFKQYAGKYILFVNVASYG----DQYLELNALQEELGPF-GLVILGFPSNQFG 102
              PF     +  + VNV  +G    ++   L  + +++ P  G+ IL FPS++FG
Sbjct: 153619 PFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQILAFPSDEFG 153461



 Score = 28.1 bits (61), Expect = 8.9
 Identities = 18/50 (36%), Positives = 27/50 (54%)
 Frame = +1

Query: 124     GFVPNFQLFEKGDVNGEKEQKFYTFLKNSCPPTAELLGSPGRLFWEPMKI 173
               G VP+ Q F +G+  GE  + F+ +        A+L+GSP     EPM+I
Sbjct: 2580583 GDVPSSQWFSEGN-GGESRKSFHGYPDGY----AQLIGSPDAWHIEPMQI 2580717


>scaffold_13
          Length = 1338096

 Score = 28.1 bits (61), Expect = 8.9
 Identities = 25/94 (26%), Positives = 35/94 (37%), Gaps = 23/94 (24%)
 Frame = -3

Query: 135    GDVNGEKEQKFYTFLKNSCPPTAELLGSPGRLFWEPM---------------KIHDIRWN 179
              G  + E EQ+ Y  +  SCP  A  + S    F  PM               KI D+  +
Sbjct: 701473 GAQSTESEQRVYVAIVCSCPRNASDVYSTKSRFSSPMKGAYWTLTWWSTPGLKIDDLTRS 701294

Query: 180    FEKFLVGPDGIP--------VMRWYHRTTVSNVK 205
              F  F+    G+P        + RW+   T   VK
Sbjct: 701293 FAAFVRACVGVPSARFRMSLLPRWFDDATTFTVK 701192


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.319    0.141    0.440 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,866,627
Number of Sequences: 1185
Number of extensions: 233429
Number of successful extensions: 826
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 44
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 578
Number of HSP's gapped (non-prelim): 498
length of query: 223
length of database: 18,886,859
effective HSP length: 100
effective length of query: 123
effective length of database: 18,768,359
effective search space: 2308508157
effective search space used: 2308508157
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000101_1.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Mus musculus # Complete
         (197 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             87   1e-17
scaffold_34                                                            85   5e-17
scaffold_69                                                            84   1e-16
scaffold_8                                                             64   9e-11
scaffold_972                                                           56   3e-08
scaffold_2                                                             40   0.002
scaffold_4                                                             28   7.1  

>scaffold_6
          Length = 2755913

 Score = 87.4 bits (215), Expect = 1e-17
 Identities = 55/141 (39%), Positives = 81/141 (57%), Gaps = 5/141 (3%)
 Frame = +2

Query: 58      KYRGFVCIVTNVASQXGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIK 117
               +YR    +V N AS+ G       +L  L ARY   GL +LAFPC+QFG QEPGS  +I+
Sbjct: 1870745 RYRRRAHLVVNTASRCGFAP-QLRELQALWARYP--GLAVLAFPCDQFGGQEPGSAGDIR 1870915

Query: 118     EFAAGYNVKFDMYSKICVNGDDAHPLWKWMKVQPKG-----RGMLGNAIKWNFTKFLIDK 172
               +    Y V F ++ K  VNG  AHPL+ ++K    G     +G+  + ++WNFTK+LI  
Sbjct: 1870916 DAYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVPNKGLPRDEVQWNFTKWLI-V 1871092

Query: 173     NGCVVKRYGPMEEPQVIEKDL 193
               +G V++RY     P+ +  D+
Sbjct: 1871093 DGEVLRRYSFDASPKSVLADV 1871155



 Score = 84.7 bits (208), Expect = 6e-17
 Identities = 63/174 (36%), Positives = 89/174 (51%), Gaps = 14/174 (8%)
 Frame = +3

Query: 38      ARSMHEFSAKDIDGH-MVCLDKYRGFVCIVTNVASQXGKTDVNYTQLVDLHARYAECG-- 94
               A S  +F+   + G   V L  Y     ++ N AS+ G T     QL    A  A+ G  
Sbjct: 2424444 AASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTP----QLGGFQALQAKFGDR 2424611

Query: 95      LRILAFPCNQFGRQEPGSNQEIKEFAAG-YNVKFDMYSKICVNGDDAHPLWKWMKVQPK- 152
               L++LAFPC+QF +QEPGS  EI  F A  + V F +++K  VNG DAHPL+ ++K   K 
Sbjct: 2424612 LQVLAFPCDQF-KQEPGSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKD 2424788

Query: 153     ---------GRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL 197
                         +G     + WNFTK+L+  +G   KRY    +P+ IE D+   L
Sbjct: 2424789 QAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAAL 2424947



 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 32/94 (34%), Positives = 50/94 (53%), Gaps = 6/94 (6%)
 Frame = +2

Query: 61      GFVCIVTNVASQXGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA 120
               G V +V NVAS  G T  ++TQ+ DL  ++A      L FP NQFG+Q    + E+    
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 121     A------GYNVKFDMYSKICVNGDDAHPLWKWMK 148
               A      G+   F +++K   NG+ A  L+K+++
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLR 1233703



 Score = 38.1 bits (87), Expect = 0.007
 Identities = 18/45 (40%), Positives = 24/45 (53%)
 Frame = +1

Query: 138     DDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGP 182
               DD   +W   +  P     +G  + WNF KFLI++ G  VKRY P
Sbjct: 1233814 DDFREIWTGKRSSPSRS--VGTDLAWNFEKFLINQEGKPVKRYSP 1233942


>scaffold_34
          Length = 430012

 Score = 85.1 bits (209), Expect = 5e-17
 Identities = 54/148 (36%), Positives = 79/148 (53%), Gaps = 13/148 (8%)
 Frame = -1

Query: 42     HEFSAKDIDGHMVCLDKYRGFVCIVTNVASQXGKTDVNYTQLVDLHARYAECGLRILAFP 101
              H+  ++DIDG  V    +RG V  VTN+AS+ GKT+ +Y  L  L A+++  GL I+ FP
Sbjct: 212218 HDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFP 212039

Query: 102    CNQFGRQEPGSNQEIKEFA------------AGYNVKFDMYSKICVNGDDAHPLWKWMKV 149
              CNQF  QEPG+  EIK FA            A     F++  K  V+G    P++ ++K 
Sbjct: 212038 CNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKR 211859

Query: 150    QPKGRGMLGNAIKWNF-TKFLIDKNGCV 176
              +   +      I WNF   FL+ ++G V
Sbjct: 211858 EAPCK------ISWNFGAYFLVSRDGAV 211793


>scaffold_69
          Length = 78151

 Score = 84.0 bits (206), Expect = 1e-16
 Identities = 45/124 (36%), Positives = 66/124 (53%), Gaps = 1/124 (0%)
 Frame = +2

Query: 66    VTNVASQXGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFA-AGYN 124
             +++  S  GKT  +Y+Q+V+LH      GL ILAFPCNQFG QEP + + + EFA + Y 
Sbjct: 68663 ISHPFSCPGKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYG 68842

Query: 125   VKFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPME 184
               F M  K+ V+G  AHP++ ++      R   G    WNF  + +      VK Y  + 
Sbjct: 68843 ADFTMMEKVAVHGPRAHPVYNFL------RATTGATPSWNFGVYFVVSRSGEVKAYPDVA 69004

Query: 185   EPQV 188
               Q+
Sbjct: 69005 PAQL 69016



 Score = 32.7 bits (73), Expect = 0.29
 Identities = 20/59 (33%), Positives = 30/59 (50%)
 Frame = +3

Query: 38    ARSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQXGKTDVNYTQLVDLHARYAECGLR 96
             A S ++  AK   G  +  D ++G V ++TNVAS  GKT  +  +  D  +   EC  R
Sbjct: 68379 ASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAH--EKSDSTSLQRECSAR 68549


>scaffold_8
          Length = 1961717

 Score = 64.3 bits (155), Expect = 9e-11
 Identities = 42/130 (32%), Positives = 67/130 (51%), Gaps = 5/130 (3%)
 Frame = +3

Query: 56      LDKYRGFVCIVTNVASQXGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQE 115
               L K+RG   +V N+         N+  L  + A YAE GLR+ AFP  Q G  EP  ++ 
Sbjct: 1062501 LSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYEPDVSEL 1062677

Query: 116     IK-----EFAAGYNVKFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLI 170
               ++     +F  G      ++ KI V G+ AHPL++++ +     G+    +  NF KFL+
Sbjct: 1062678 VRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLALATNPNGV--GRLTVNFEKFLL 1062851

Query: 171     DKNGCVVKRY 180
               D +G  ++RY
Sbjct: 1062852 DGDGKPLRRY 1062881


>scaffold_972
          Length = 3742

 Score = 55.8 bits (133), Expect = 3e-08
 Identities = 42/135 (31%), Positives = 62/135 (45%), Gaps = 3/135 (2%)
 Frame = +3

Query: 50   DGHMVCLDKYRGFVCIVTNVASQXGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQE 109
            DG  V   ++ G VC   NVAS  G T   Y  +  L   + +  L ++AFP  +FG QE
Sbjct: 3276 DGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIAFPTGEFGGQE 3452

Query: 110  PGSNQEIKEFAAGYNVKFDMYSK--ICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFT- 166
              +++ I +FA    VKF    +  +    D     W+ M+ +        +A  WNF  
Sbjct: 3453 LPTDEAIAKFAYD-RVKFPAAPRGVLLQKADLGRASWQAMQAEAG-----ADAPAWNFKG 3614

Query: 167  KFLIDKNGCVVKRYG 181
            KFL+ K+G V    G
Sbjct: 3615 KFLVSKDGAVTDASG 3659


>scaffold_2
          Length = 3465789

 Score = 40.0 bits (92), Expect = 0.002
 Identities = 21/61 (34%), Positives = 39/61 (63%), Gaps = 5/61 (8%)
 Frame = -3

Query: 94     GLRILAFPCNQFGRQEPGSNQEIKEFAAGYNV-----KFDMYSKICVNGDDAHPLWKWMK 148
              G++ILAFP ++FG +   ++QE  +F     V      F + +K+ V+G DAHP++K+++
Sbjct: 153499 GVQILAFPSDEFGPK--FTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLR 153326

Query: 149    V 149
              +
Sbjct: 153325 L 153323


>scaffold_4
          Length = 3024044

 Score = 28.1 bits (61), Expect = 7.1
 Identities = 14/27 (51%), Positives = 17/27 (62%)
 Frame = -1

Query: 33      DDWRCARSMHEFSAKDIDGHMVCLDKY 59
               DD RCAR   E  A  +DG ++CLD Y
Sbjct: 2438084 DDGRCAR---EIDATLLDGLVLCLDTY 2438013


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.324    0.139    0.462 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,698,605
Number of Sequences: 1185
Number of extensions: 174330
Number of successful extensions: 912
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 39
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 667
Number of HSP's gapped (non-prelim): 486
length of query: 197
length of database: 18,886,859
effective HSP length: 99
effective length of query: 98
effective length of database: 18,769,544
effective search space: 1839415312
effective search space used: 1839415312
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000102_1.0 # Protein # Glutathione peroxidase 5 (GPx5) #
Mus musculus # Complete
         (237 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             90   2e-18
scaffold_34                                                            59   4e-09
scaffold_69                                                            43   4e-04
scaffold_972                                                           39   0.004
scaffold_8                                                             37   0.028
scaffold_13                                                            34   0.18 

>scaffold_6
          Length = 2755913

 Score = 90.1 bits (222), Expect = 2e-18
 Identities = 56/142 (39%), Positives = 77/142 (54%), Gaps = 11/142 (7%)
 Frame = +2

Query: 77      GKHVLFVNVATYCGLTIQ-YPELNALQEDLKPFGLVILGFPCNQFGKQEPGDNLEILPGL 135
               G+ VL  NVA+  G T++ + ++N L E         LGFP NQFGKQ    + E+LP L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 136     KYVRPGKGFLPNFQLFAKGDVNGENEQKIFTFLKRSCPHPSET--------VVMSKHTFW 187
                +VRPG GF+PNF +F K + NGE    +F FL+ S   PS+         V+ +K+  W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 188     EPIKVHDIR--WNFEKFLVGPD 207
                P+   D R    FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 82.8 bits (203), Expect = 3e-16
 Identities = 61/177 (34%), Positives = 83/177 (46%), Gaps = 2/177 (1%)
 Frame = +3

Query: 58      DYEALSLNGKEHIPFKQYAGKHVLFVNVATYCGLTIQYPELNALQEDLKPFG--LVILGF 115
               D+    L G   +    YA +  L  N A+ CG+T   P+L   Q     FG  L +L F
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMT---PQLGGFQALQAKFGDRLQVLAF 2424629

Query: 116     PCNQFGKQEPGDNLEILPGLKYVRPGKGFLPNFQLFAKGDVNGENEQKIFTFLKRSCPHP 175
               PC+QF KQEPG   EI            F   F LFAKGDVNG +   +F FLK +    
Sbjct: 2424630 PCDQF-KQEPGSPAEIAAFY-----ADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQ 2424791

Query: 176     SETVVMSKHTFWEPIKVHDIRWNFEKFLVGPDGIPVMRWFHQAPVSTVKSDIMAYLS 232
               +  V    +   +     D+ WNF K+LV  DG P  R+ +      +++DI A L+
Sbjct: 2424792 AAPVPPWNN---KGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAALN 2424950



 Score = 79.7 bits (195), Expect = 3e-15
 Identities = 58/177 (32%), Positives = 88/177 (49%), Gaps = 3/177 (1%)
 Frame = +2

Query: 55      TIYDYEALSLNGKEHIPFKQYAGKHVLFVNVATYCGLTIQYPELNALQEDLKPFGLVILG 114
               T +D+E + L G   +   +Y  +  L VN A+ CG   Q  EL AL       GL +L 
Sbjct: 1870688 TYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLA 1870861

Query: 115     FPCNQFGKQEPGDNLEILPGLKYVRPGKGFLPNFQLFAKGDVNGENEQKIFTFLKRSC-- 172
               FPC+QFG QEPG   +I     Y R G     +F +F K DVNG     +FT+LK++   
Sbjct: 1870862 FPCDQFGGQEPGSAGDIRDA--YDRYG----VDFPVFEKSDVNGPRAHPLFTYLKKATAG 1871023

Query: 173     -PHPSETVVMSKHTFWEPIKVHDIRWNFEKFLVGPDGIPVMRWFHQAPVSTVKSDIM 228
                P P++ +              +++WNF K+L+  DG  + R+   A   +V +D++
Sbjct: 1871024 QPVPNKGLPRD-----------EVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVV 1871158



 Score = 38.9 bits (89), Expect = 0.006
 Identities = 17/40 (42%), Positives = 24/40 (60%)
 Frame = +1

Query: 194     DIRWNFEKFLVGPDGIPVMRWFHQAPVSTVKSDIMAYLSH 233
               D+ WNFEKFL+  +G PV R+      + V +D+ A L H
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALLEH 1233993


>scaffold_34
          Length = 430012

 Score = 59.3 bits (142), Expect = 4e-09
 Identities = 54/167 (32%), Positives = 69/167 (41%), Gaps = 12/167 (7%)
 Frame = -1

Query: 54     GTIYDYEALSLNGKEHIPFKQYAGKHVLFVNVATYCGLT-IQYPELNALQEDLKPFGLVI 112
              G  +D  +  ++G E + F  + G+ V   N+A+  G T   Y  L  L       GL I
Sbjct: 212227 GGFHDLMSEDIDGAE-VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 113    LGFPCNQFGKQEPGDNLEILPGLKYVRPGKGFLP----------NFQLFAKGDVNGENEQ 162
              +GFPCNQF  QEPG   EI    K     +GFL            F L  K DV+G    
Sbjct: 212050 VGFPCNQFMGQEPGAESEI----KAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVD 211883

Query: 163    KIFTFLKRSCPHPSETVVMSKHTFWEPIKVHDIRWNF-EKFLVGPDG 208
               +F++LKR  P                     I WNF   FLV  DG
Sbjct: 211882 PVFSYLKREAP-------------------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 42.7 bits (99), Expect = 4e-04
 Identities = 25/75 (33%), Positives = 40/75 (53%)
 Frame = +2

Query: 95    YPELNALQEDLKPFGLVILGFPCNQFGKQEPGDNLEILPGLKYVRPGKGFLPNFQLFAKG 154
             Y ++  L ++L   GL IL FPCNQFG QEP    E++   ++ +   G   +F +  K 
Sbjct: 68705 YSQMVELHDELGHEGLEILAFPCNQFGNQEPA-TCEVV--AEFAKSAYG--ADFTMMEKV 68869

Query: 155   DVNGENEQKIFTFLK 169
              V+G     ++ FL+
Sbjct: 68870 AVHGPRAHPVYNFLR 68914


>scaffold_972
          Length = 3742

 Score = 39.3 bits (90), Expect = 0.004
 Identities = 45/161 (27%), Positives = 63/161 (39%), Gaps = 4/161 (2%)
 Frame = +3

Query: 52   VKGTIYDYEALSLNGKEHIPFKQYAGKHVLFVNVATYCGLTIQYPELNALQEDLKPFG-- 109
            V+   YD   ++ +G E + F Q+ GK    VNVA+  G T   P    +++    FG  
Sbjct: 3237 VRAAFYDLADVANDGSE-VAFSQFEGKVCYAVNVASA*GAT--RPGYKLMKQLSDEFGDD 3407

Query: 110  LVILGFPCNQFGKQE-PGDNLEILPGLKYVRPGKGFLPNFQLFAKGDVNGENEQKIFTFL 168
            L ++ FP  +FG QE P D  E +    Y R      P   L  K D+   + Q +    
Sbjct: 3408 LAVIAFPTGEFGGQELPTD--EAIAKFAYDRVKFPAAPRGVLLQKADLGRASWQAMQAEA 3581

Query: 169  KRSCPHPSETVVMSKHTFWEPIKVHDIRWNFE-KFLVGPDG 208
                P                       WNF+ KFLV  DG
Sbjct: 3582 GADAP----------------------AWNFKGKFLVSKDG 3638


>scaffold_8
          Length = 1961717

 Score = 36.6 bits (83), Expect = 0.028
 Identities = 32/106 (30%), Positives = 45/106 (42%)
 Frame = +3

Query: 109     GLVILGFPCNQFGKQEPGDNLEILPGLKYVRPGKGFLPNFQLFAKGDVNGENEQKIFTFL 168
               GL +  FP  Q G  EP D  E++    Y + G G  P   +F K DV G     ++ +L
Sbjct: 1062615 GLRVWAFPTEQ-GYYEP-DVSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYL 1062788

Query: 169     KRSCPHPSETVVMSKHTFWEPIKVHDIRWNFEKFLVGPDGIPVMRW 214
                 +                 P  V  +  NFEKFL+  DG P+ R+
Sbjct: 1062789 ALAT---------------NPNGVGRLTVNFEKFLLDGDGKPLRRY 1062881


>scaffold_13
          Length = 1338096

 Score = 33.9 bits (76), Expect = 0.18
 Identities = 24/94 (25%), Positives = 39/94 (41%), Gaps = 23/94 (24%)
 Frame = -3

Query: 154    GDVNGENEQKIFTFLKRSCPHPSETVVMSKHTFWEP---------------IKVHDIRWN 198
              G  + E+EQ+++  +  SCP  +  V  +K  F  P               +K+ D+  +
Sbjct: 701473 GAQSTESEQRVYVAIVCSCPRNASDVYSTKSRFSSPMKGAYWTLTWWSTPGLKIDDLTRS 701294

Query: 199    FEKFLVGPDGIP--------VMRWFHQAPVSTVK 224
              F  F+    G+P        + RWF  A   TVK
Sbjct: 701293 FAAFVRACVGVPSARFRMSLLPRWFDDATTFTVK 701192


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.323    0.140    0.441 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,696,683
Number of Sequences: 1185
Number of extensions: 212048
Number of successful extensions: 627
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 28
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 523
Number of HSP's gapped (non-prelim): 241
length of query: 237
length of database: 18,886,859
effective HSP length: 101
effective length of query: 136
effective length of database: 18,767,174
effective search space: 2552335664
effective search space used: 2552335664
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000103_1.0 # Protein # Glutathione peroxidase 6 (GPx6) #
Mus musculus # Complete
         (221 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             88   7e-18
scaffold_34                                                            61   9e-10
scaffold_69                                                            46   4e-05
scaffold_972                                                           39   0.006
scaffold_8                                                             38   0.011

>scaffold_6
          Length = 2755913

 Score = 88.2 bits (217), Expect = 7e-18
 Identities = 56/142 (39%), Positives = 78/142 (54%), Gaps = 11/142 (7%)
 Frame = +2

Query: 61      GKHILFVNVASFCGLTAT-YPELNTLQEELKPFNVTVLGFPCNQFGKQEPGKNSEILLGL 119
               G+ +L  NVAS  G T   + ++N L E+      T LGFP NQFGKQ   K+ E+L  L
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 120     KYVRPGGGYVPNFQLFEKGDVNGDNEQKVFSFLKNSCPPTSELFG--------SPEHLFW 171
                +VRPGGG+VPNF +F K + NG+    +F FL++S    S+ F         S +++ W
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPSDDFKGQGSDYVISTKNIIW 1233781

Query: 172     DPMKVHDIR--WNFEKFLVGPD 191
                P+   D R    FE+F  G D
Sbjct: 1233782 TPVTRRDQRPVTTFERFGPGKD 1233847



 Score = 78.6 bits (192), Expect = 6e-15
 Identities = 59/175 (33%), Positives = 80/175 (45%)
 Frame = +3

Query: 42      EYGANTIDGGEFVNFQQYAGKHILFVNVASFCGLTATYPELNTLQEELKPFNVTVLGFPC 101
               ++    + GG  V    YA +  L  N AS CG+T        LQ +     + VL FPC
Sbjct: 2424459 DFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQLGGFQALQAKFGD-RLQVLAFPC 2424635

Query: 102     NQFGKQEPGKNSEILLGLKYVRPGGGYVPNFQLFEKGDVNGDNEQKVFSFLKNSCPPTSE 161
               +QF KQEPG  +EI     Y    G     F LF KGDVNG +   +F+FLK +   T +
Sbjct: 2424636 DQF-KQEPGSPAEI--AAFYADKFG---VTFPLFAKGDVNGPDAHPLFAFLKEA---TKD 2424788

Query: 162     LFGSPEHLFWDPMKVHDIRWNFEKFLVGPDGVPVMRWFHHTPVRIVQSDIMEYLN 216
                               D+ WNF K+LV  DG P  R+ +      +++DI   LN
Sbjct: 2424789 QAAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAALN 2424950



 Score = 68.2 bits (165), Expect = 8e-12
 Identities = 54/174 (31%), Positives = 81/174 (46%)
 Frame = +2

Query: 39      TVYEYGANTIDGGEFVNFQQYAGKHILFVNVASFCGLTATYPELNTLQEELKPFNVTVLG 98
               T +++    + GG  +   +Y  +  L VN AS CG      EL  L        + VL 
Sbjct: 1870688 TYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLA 1870861

Query: 99      FPCNQFGKQEPGKNSEILLGLKYVRPGGGYVPNFQLFEKGDVNGDNEQKVFSFLKNSCPP 158
               FPC+QFG QEPG   +I     Y R    Y  +F +FEK DVNG     +F++LK +   
Sbjct: 1870862 FPCDQFGGQEPGSAGDIRDA--YDR----YGVDFPVFEKSDVNGPRAHPLFTYLKKATA- 1871020

Query: 159     TSELFGSPEHLFWDPMKVHDIRWNFEKFLVGPDGVPVMRWFHHTPVRIVQSDIM 212
                    G P      P    +++WNF K+L+  DG  + R+      + V +D++
Sbjct: 1871021 -----GQPVPNKGLPR--DEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVV 1871158



 Score = 33.5 bits (75), Expect = 0.21
 Identities = 12/21 (57%), Positives = 16/21 (76%)
 Frame = +1

Query: 178     DIRWNFEKFLVGPDGVPVMRW 198
               D+ WNFEKFL+  +G PV R+
Sbjct: 1233874 DLAWNFEKFLINQEGKPVKRY 1233936


>scaffold_34
          Length = 430012

 Score = 61.2 bits (147), Expect = 9e-10
 Identities = 54/163 (33%), Positives = 71/163 (43%), Gaps = 8/163 (4%)
 Frame = -1

Query: 38     GTVYEYGANTIDGGEFVNFQQYAGKHILFVNVASFCGLTAT-YPELNTLQEELKPFNVTV 96
              G  ++  +  IDG E V F  + G+ +   N+AS  G T + Y  L  L  +     + +
Sbjct: 212227 GGFHDLMSEDIDGAE-VRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAI 212051

Query: 97     LGFPCNQFGKQEPGKNSEILLGLK---YVRPGGG---YVPNFQLFEKGDVNGDNEQKVFS 150
              +GFPCNQF  QEPG  SEI    +   ++ P  G    V  F L  K DV+G     VFS
Sbjct: 212050 VGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFS 211871

Query: 151    FLKNSCPPTSELFGSPEHLFWDPMKVHDIRWNF-EKFLVGPDG 192
              +LK   P                     I WNF   FLV  DG
Sbjct: 211870 YLKREAP-------------------CKISWNFGAYFLVSRDG 211799


>scaffold_69
          Length = 78151

 Score = 45.8 bits (107), Expect = 4e-05
 Identities = 26/77 (33%), Positives = 38/77 (49%)
 Frame = +2

Query: 77    ATYPELNTLQEELKPFNVTVLGFPCNQFGKQEPGKNSEILLGLKYVRPGGGYVPNFQLFE 136
             A Y ++  L +EL    + +L FPCNQFG QEP     +    K       Y  +F + E
Sbjct: 68699 AHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAK-----SAYGADFTMME 68863

Query: 137   KGDVNGDNEQKVFSFLK 153
             K  V+G     V++FL+
Sbjct: 68864 KVAVHGPRAHPVYNFLR 68914


>scaffold_972
          Length = 3742

 Score = 38.5 bits (88), Expect = 0.006
 Identities = 42/150 (28%), Positives = 58/150 (38%), Gaps = 2/150 (1%)
 Frame = +3

Query: 45   ANTIDGGEFVNFQQYAGKHILFVNVASFCGLTAT-YPELNTLQEELKPFNVTVLGFPCNQ 103
            A+  + G  V F Q+ GK    VNVAS  G T   Y  +  L +E    ++ V+ FP  +
Sbjct: 3261 ADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIAFPTGE 3437

Query: 104  FGKQEPGKNSEILLGLKYVRPGGGYVPNFQLFEKGDVNGDNEQKVFSFLKNSCPPTSELF 163
            FG QE     E +    Y R      P   L +K D+   + Q + +      P      
Sbjct: 3438 FGGQEL-PTDEAIAKFAYDRVKFPAAPRGVLLQKADLGRASWQAMQAEAGADAP------ 3596

Query: 164  GSPEHLFWDPMKVHDIRWNFE-KFLVGPDG 192
                             WNF+ KFLV  DG
Sbjct: 3597 ----------------AWNFKGKFLVSKDG 3638


>scaffold_8
          Length = 1961717

 Score = 37.7 bits (86), Expect = 0.011
 Identities = 32/103 (31%), Positives = 46/103 (44%)
 Frame = +3

Query: 96      VLGFPCNQFGKQEPGKNSEILLGLKYVRPGGGYVPNFQLFEKGDVNGDNEQKVFSFLKNS 155
               V  FP  Q G  EP   SE++    Y + G G  P   +F+K DV G+    ++ +L   
Sbjct: 1062624 VWAFPTEQ-GYYEPDV-SELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLA-- 1062791

Query: 156     CPPTSELFGSPEHLFWDPMKVHDIRWNFEKFLVGPDGVPVMRW 198
                            L  +P  V  +  NFEKFL+  DG P+ R+
Sbjct: 1062792 -------------LATNPNGVGRLTVNFEKFLLDGDGKPLRRY 1062881


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.139    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,829,128
Number of Sequences: 1185
Number of extensions: 216633
Number of successful extensions: 654
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 24
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 560
Number of HSP's gapped (non-prelim): 234
length of query: 221
length of database: 18,886,859
effective HSP length: 100
effective length of query: 121
effective length of database: 18,768,359
effective search space: 2270971439
effective search space used: 2270971439
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000104_1.0 # Protein # Glutathione peroxidase 7 (GPx7) #
Mus musculus # Complete
         (186 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_69                                                            89   3e-18
scaffold_6                                                             83   2e-16
scaffold_34                                                            77   2e-14
scaffold_8                                                             67   1e-11
scaffold_972                                                           62   5e-10
scaffold_2                                                             47   1e-05

>scaffold_69
          Length = 78151

 Score = 89.0 bits (219), Expect = 3e-18
 Identities = 43/107 (40%), Positives = 56/107 (52%)
 Frame = +2

Query: 57    GFTDQNYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDTNREIENFARRTYSVSFPMFSK 116
             G T  +Y  + +L  +LG     +LAFPCNQFG QEP T   +  FA+  Y   F M  K
Sbjct: 68687 GKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEK 68866

Query: 117   IAVTGTGAHPAFKYLTQTSGKEPTWNFWKYLVDPDGKVVGAWDPTVP 163
             +AV G  AHP + +L  T+G  P+WNF  Y V      V A+    P
Sbjct: 68867 VAVHGPRAHPVYNFLRATTGATPSWNFGVYFVVSRSGEVKAYPDVAP 69007



 Score = 32.3 bits (72), Expect = 0.34
 Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
 Frame = +3

Query: 24    FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRA-LQQLQRDLGPHHF 78
             FYD KA    G  ++ + ++G V L+ NVAS  G T  + ++    LQR+     F
Sbjct: 68388 FYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAHEKSDSTSLQRECSARAF 68555


>scaffold_6
          Length = 2755913

 Score = 82.8 bits (203), Expect = 2e-16
 Identities = 59/145 (40%), Positives = 78/145 (53%), Gaps = 13/145 (8%)
 Frame = +2

Query: 24      FYDFKAVNIRGKL-VSLEKYRGSVSLVVNVASECGFTDQNYRALQQL-QRDLGPHHFNVL 81
               ++DF+ V + G   ++  +YR    LVVN AS CGF  Q  R LQ L  R  G     VL
Sbjct: 1870691 YWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ-LRELQALWARYPG---LAVL 1870858

Query: 82      AFPCNQFGQQEPDTNREIENFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTQTSGKEPT- 140
               AFPC+QFG QEP +  +I +   R Y V FP+F K  V G  AHP F YL + +  +P  
Sbjct: 1870859 AFPCDQFGGQEPGSAGDIRDAYDR-YGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVP 1871035

Query: 141     ----------WNFWKYLVDPDGKVV 155
                         WNF K+L+  DG+V+
Sbjct: 1871036 NKGLPRDEVQWNFTKWLI-VDGEVL 1871107



 Score = 73.6 bits (179), Expect = 1e-13
 Identities = 50/131 (38%), Positives = 65/131 (49%), Gaps = 16/131 (12%)
 Frame = +3

Query: 34      GKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQRDLGPHHFNVLAFPCNQFGQQEP 93
               G  V+L  Y     L+ N AS+CG T Q     Q LQ   G     VLAFPC+QF +QEP
Sbjct: 2424486 GGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR-LQVLAFPCDQF-KQEP 2424656

Query: 94      DTNREIENFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTQTS-------------GKEPT 140
                +  EI  F    + V+FP+F+K  V G  AHP F +L + +             G+  T
Sbjct: 2424657 GSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPPWNNKGQAAT 2424836

Query: 141     ---WNFWKYLV 148
                  WNF K+LV
Sbjct: 2424837 DVMWNFTKWLV 2424869



 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 32/98 (32%), Positives = 46/98 (46%), Gaps = 5/98 (5%)
 Frame = +2

Query: 44      GSVSLVVNVASECGFTDQNYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDTNREIENFA 103
               G V LV NVAS  G T +++  +  L        F  L FP NQFG+Q  + + E+    
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 104     RRT-----YSVSFPMFSKIAVTGTGAHPAFKYLTQTSG 136
                       +  +FP+F+K    G GA   FK+L  + G
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLG 1233715



 Score = 29.3 bits (64), Expect = 2.9
 Identities = 11/36 (30%), Positives = 22/36 (61%)
 Frame = +1

Query: 132     TQTSGKEPTWNFWKYLVDPDGKVVGAWDPTVPVAEI 167
               +++ G +  WNF K+L++ +GK V  + P    A++
Sbjct: 1233856 SRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADV 1233963


>scaffold_34
          Length = 430012

 Score = 76.6 bits (187), Expect = 2e-14
 Identities = 48/143 (33%), Positives = 68/143 (47%), Gaps = 12/143 (8%)
 Frame = -1

Query: 24     FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQRDLGPHHFNVLAF 83
              F+D  + +I G  V    +RG V  V N+AS  G T+ +Y+ L +L          ++ F
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 84     PCNQFGQQEPDTNREIENFARRTYSV-----------SFPMFSKIAVTGTGAHPAFKYLT 132
              PCNQF  QEP    EI+ FAR    +           +F +  K  V+G    P F YL 
Sbjct: 212041 PCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLK 211862

Query: 133    QTSGKEPTWNFWKY-LVDPDGKV 154
              + +  + +WNF  Y LV  DG V
Sbjct: 211861 REAPCKISWNFGAYFLVSRDGAV 211793


>scaffold_8
          Length = 1961717

 Score = 67.0 bits (162), Expect = 1e-11
 Identities = 46/133 (34%), Positives = 64/133 (48%), Gaps = 9/133 (6%)
 Frame = +3

Query: 30      VNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQRDLGPHHFNVLAFPCNQFG 89
               V  +GK   L K+RGS  LVVN+  +     +N+ AL+ +  +       V AFP  Q G
Sbjct: 1062474 VPFKGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-G 1062650

Query: 90      QQEPDTN-----REIENFARRTYSVSFPMFSKIAVTGTGAHPAFKYLTQTSGKEP----T 140
                 EPD +     +    F   TY  +  +F KI V G  AHP ++YL   +        T
Sbjct: 1062651 YYEPDVSELVRAKAYSQFGFGTYPTAV-VFDKIDVIGNTAHPLYRYLALATNPNGVGRLT 1062827

Query: 141     WNFWKYLVDPDGK 153
                NF K+L+D DGK
Sbjct: 1062828 VNFEKFLLDGDGK 1062866


>scaffold_972
          Length = 3742

 Score = 61.6 bits (148), Expect = 5e-10
 Identities = 48/140 (34%), Positives = 64/140 (45%), Gaps = 6/140 (4%)
 Frame = +3

Query: 24   FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQRDLGPHHFNVLAF 83
            FYD   V   G  V+  ++ G V   VNVAS  G T   Y+ ++QL  + G     V+AF
Sbjct: 3249 FYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFG-DDLAVIAF 3425

Query: 84   PCNQFGQQEPDTNREIENFARRTYSVSFP-----MFSKIAVTGTGAHPAFKYLTQTSGKE 138
            P  +FG QE  T+  I  FA     V FP     +  + A  G  +  A +   +     
Sbjct: 3426 PTGEFGGQELPTDEAIAKFAYD--RVKFPAAPRGVLLQKADLGRASWQAMQ--AEAGADA 3593

Query: 139  PTWNF-WKYLVDPDGKVVGA 157
            P WNF  K+LV  DG V  A
Sbjct: 3594 PAWNFKGKFLVSKDGAVTDA 3653


>scaffold_2
          Length = 3465789

 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 35/120 (29%), Positives = 53/120 (44%), Gaps = 5/120 (4%)
 Frame = -3

Query: 24     FYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQNYRALQQLQRDLGP-HHFNVLA 82
              FY   A   RG     +       ++VNV        +   AL+++  D+ P     +LA
Sbjct: 153661 FYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQILA 153482

Query: 83     FPCNQFGQQEPDTNREIENF----ARRTYSVSFPMFSKIAVTGTGAHPAFKYLTQTSGKE 138
              FP ++FG +   T++E  +F           SF + +K+ V G  AHP FKYL   S  E
Sbjct: 153481 FPSDEFGPKF--TDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLRLMSNTE 153308


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.136    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,951,188
Number of Sequences: 1185
Number of extensions: 140161
Number of successful extensions: 552
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 51
Number of HSP's successfully gapped in prelim test: 15
Number of HSP's that attempted gapping in prelim test: 391
Number of HSP's gapped (non-prelim): 325
length of query: 186
length of database: 18,886,859
effective HSP length: 98
effective length of query: 88
effective length of database: 18,770,729
effective search space: 1651824152
effective search space used: 1651824152
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000105_1.0 # Protein # Glutathione peroxidase 8 (GPx8) #
Mus musculus # Complete
         (209 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_69                                                            71   8e-13
scaffold_6                                                             70   2e-12
scaffold_34                                                            54   1e-07
scaffold_8                                                             49   3e-06
scaffold_972                                                           47   1e-05
scaffold_2                                                             37   0.017
scaffold_13                                                            29   4.7  

>scaffold_69
          Length = 78151

 Score = 71.2 bits (173), Expect = 8e-13
 Identities = 36/107 (33%), Positives = 48/107 (44%)
 Frame = +2

Query: 82    TDKSYQTLRELHKEFGPYHFNVLAFPCNQFGXXXXXXXXXXXXFARQNYGVTFPIFHKIK 141
             T   Y  + ELH E G     +LAFPCNQFG            FA+  YG  F +  K+ 
Sbjct: 68693 TAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEKVA 68872

Query: 142   ILGPEAEPAFRFIVDSSKKEPRWNFWKYLVNPEGQVVKFWRPEEPLE 188
             + GP A P + F+  ++   P WNF  Y V      VK +    P +
Sbjct: 68873 VHGPRAHPVYNFLRATTGATPSWNFGVYFVVSRSGEVKAYPDVAPAQ 69013



 Score = 36.2 bits (82), Expect = 0.029
 Identities = 16/33 (48%), Positives = 22/33 (66%)
 Frame = +3

Query: 45    NSFYSFEVKDAKGRTVSLEKFKGKASLVVNVAS 77
             +SFY  + K A G T++ + FKGK  L+ NVAS
Sbjct: 68382 SSFYDLKAKTAAGATLTFDAFKGKVVLLTNVAS 68480


>scaffold_6
          Length = 2755913

 Score = 69.7 bits (169), Expect = 2e-12
 Identities = 49/150 (32%), Positives = 75/150 (50%), Gaps = 13/150 (8%)
 Frame = +2

Query: 43      RTNSFYSFE-VKDAKGRTVSLEKFKGKASLVVNVASDCRFTDKSYQTLRELHKEFGPYH- 100
               R  +++ FE V    G  ++  +++ +A LVVN AS C F  +    LREL   +  Y  
Sbjct: 1870679 RLRTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFAPQ----LRELQALWARYPG 1870846

Query: 101     FNVLAFPCNQFGXXXXXXXXXXXXFARQNYGVTFPIFHKIKILGPEAEPAFRFIVDSSKK 160
                 VLAFPC+QFG             A   YGV FP+F K  + GP A P F ++  ++  
Sbjct: 1870847 LAVLAFPCDQFGGQEPGSAGDIRD-AYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAG 1871023

Query: 161     EP-----------RWNFWKYLVNPEGQVVK 179
               +P           +WNF K+L+  +G+V++
Sbjct: 1871024 QPVPNKGLPRDEVQWNFTKWLI-VDGEVLR 1871110



 Score = 63.5 bits (153), Expect = 2e-10
 Identities = 47/155 (30%), Positives = 71/155 (45%), Gaps = 16/155 (10%)
 Frame = +3

Query: 57      GRTVSLEKFKGKASLVVNVASDCRFTDKSYQTLRELHKEFGPYHFNVLAFPCNQFGXXXX 116
               G  V+L  +  +A L+ N AS C  T +     + L  +FG     VLAFPC+QF     
Sbjct: 2424486 GGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGD-RLQVLAFPCDQFKQEPG 2424659

Query: 117     XXXXXXXXFARQNYGVTFPIFHKIKILGPEAEPAFRFIVDSSKKEP-------------- 162
                       +A   +GVTFP+F K  + GP+A P F F+ +++K +               
Sbjct: 2424660 SPAEIAAFYA-DKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPPWNNKGQAAT 2424836

Query: 163     --RWNFWKYLVNPEGQVVKFWRPEEPLEAIRPHVS 195
                  WNF K+LV  +G   K +  +   EAI   ++
Sbjct: 2424837 DVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIA 2424938



 Score = 37.0 bits (84), Expect = 0.017
 Identities = 36/134 (26%), Positives = 53/134 (39%), Gaps = 14/134 (10%)
 Frame = +2

Query: 38      KFLKPRTNSFYSFEVKDAKGRTVSL---------EKFKGKASLVVNVASDCRFTDKSYQT 88
               +  +PR  +  S  V+D  GR + L          +  G+  LV NVAS    T + +  
Sbjct: 1233317 RIARPRRGAVAS--VRDDAGRKIPLFRAARAPHRRRSLGQVVLVENVASI*GTTVRDFTQ 1233490

Query: 89      LRELHKEFGPYHFNVLAFPCNQFGXXXXXXXXXXXXFARQ-----NYGVTFPIFHKIKIL 143
               + +L ++F    F  L FP NQFG                      +   FPIF K +  
Sbjct: 1233491 MNDLTEKFAG-KFTCLGFPTNQFGKQTNEKDWELLPMLAHVRPGGGFVPNFPIFTKTEAN 1233667

Query: 144     GPEAEPAFRFIVDS 157
               G  A   F+F+  S
Sbjct: 1233668 GEGASDLFKFLRSS 1233709



 Score = 30.4 bits (67), Expect = 1.6
 Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 8/48 (16%)
 Frame = +1

Query: 144     GPEAEPAFRFIVDSSKKEPR--------WNFWKYLVNPEGQVVKFWRP 183
               GP +   FR I    +  P         WNF K+L+N EG+ VK + P
Sbjct: 1233799 GPASCDDFREIWTGKRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYSP 1233942


>scaffold_34
          Length = 430012

 Score = 54.3 bits (129), Expect = 1e-07
 Identities = 47/200 (23%), Positives = 77/200 (38%), Gaps = 14/200 (7%)
 Frame = -1

Query: 16     AKIFAVLLSMVLCTVMLFLLQLKFLKP---RTNSFYSFEVKDAKGRTVSLEKFKGKASLV 72
              A   A LLS V  T+ +F  +    +P       F+    +D  G  V    F+G+   V
Sbjct: 212311 AATIAKLLSRVARTMGVFASK----EPPCAHVGGFHDLMSEDIDGAEVRFSAFRGQVVCV 212144

Query: 73     VNVASDCRFTDKSYQTLRELHKEFGPYHFNVLAFPCNQFGXXXXXXXXXXXXFARQNYGV 132
               N+AS    T+  Y+ L +L  +F      ++ FPCNQF             FAR    +
Sbjct: 212143 TNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFPCNQFMGQEPGAESEIKAFARSRGFL 211964

Query: 133    -----------TFPIFHKIKILGPEAEPAFRFIVDSSKKEPRWNFWKYLVNPEGQVVKFW 181
                          F +  K  + G   +P F ++   +  +  WNF  Y +      V+  
Sbjct: 211963 DPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKREAPCKISWNFGAYFLVSRDGAVEAH 211784

Query: 182    RPEEPLEAIRPHVSQMIGQI 201
                  P + + P V  ++G +
Sbjct: 211783 ENAHPRD-LAPRVEALLGAV 211727


>scaffold_8
          Length = 1961717

 Score = 49.3 bits (116), Expect = 3e-06
 Identities = 36/132 (27%), Positives = 61/132 (46%), Gaps = 8/132 (6%)
 Frame = +3

Query: 56      KGRTVSLEKFKGKASLVVNVASDCRFTDKSYQTLRELHKEFGPYHFNVLAFPCNQFGXXX 115
               KG+   L KF+G A LVVN+  D     +++  LR +   +      V AFP  Q G   
Sbjct: 1062483 KGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYE 1062659

Query: 116     XXXXXXXXXFARQNYGV-TFP---IFHKIKILGPEAEPAFRFIVDSSKKEP----RWNFW 167
                         A   +G  T+P   +F KI ++G  A P +R++  ++          NF 
Sbjct: 1062660 PDVSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLALATNPNGVGRLTVNFE 1062839

Query: 168     KYLVNPEGQVVK 179
               K+L++ +G+ ++
Sbjct: 1062840 KFLLDGDGKPLR 1062875


>scaffold_972
          Length = 3742

 Score = 47.4 bits (111), Expect = 1e-05
 Identities = 42/142 (29%), Positives = 61/142 (42%), Gaps = 6/142 (4%)
 Frame = +3

Query: 42   PRTNSFYSFEVKDAKGRTVSLEKFKGKASLVVNVASDCRFTDKSYQTLRELHKEFGPYHF 101
            P   +FY        G  V+  +F+GK    VNVAS    T   Y+ +++L  EFG    
Sbjct: 3234 PVRAAFYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DL 3410

Query: 102  NVLAFPCNQFGXXXXXXXXXXXXFARQNYGVTFP-----IFHKIKILGPEAEPAFRFIVD 156
             V+AFP  +FG            FA     V FP     +  +   LG  +  A +   +
Sbjct: 3411 AVIAFPTGEFGGQELPTDEAIAKFAYDR--VKFPAAPRGVLLQKADLGRASWQAMQ--AE 3578

Query: 157  SSKKEPRWNF-WKYLVNPEGQV 177
            +    P WNF  K+LV+ +G V
Sbjct: 3579 AGADAPAWNFKGKFLVSKDGAV 3644


>scaffold_2
          Length = 3465789

 Score = 37.0 bits (84), Expect = 0.017
 Identities = 23/115 (20%), Positives = 48/115 (41%), Gaps = 3/115 (2%)
 Frame = -3

Query: 43     RTNSFYSFEVKDAKGRTVSLEKFKGKASLVVNVASDCRFTDKSYQTLRELHKEFGPYH-F 101
              +T+ FY      A+G     +    +  ++VNV        +    L+ +  +  P    
Sbjct: 153673 KTSRFYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGV 153494

Query: 102    NVLAFPCNQFGXXXXXXXXXXXXFARQNY--GVTFPIFHKIKILGPEAEPAFRFI 154
               +LAFP ++FG             +   +    +F +  K+++ GP+A P F+++
Sbjct: 153493 QILAFPSDEFGPKFTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYL 153329


>scaffold_13
          Length = 1338096

 Score = 28.9 bits (63), Expect = 4.7
 Identities = 23/80 (28%), Positives = 36/80 (45%), Gaps = 4/80 (5%)
 Frame = -3

Query: 16      AKIFAVLLSMVLCTVMLFLLQLKFLKPRTNSFYSFEVKDAKGRTVSLEK----FKGKASL 71
               A +  V LSM++C +++F + + F+   T S  SF    A GRT  +E       G +  
Sbjct: 1223446 ALLRCVYLSMIICGLVVFAVLVGFI---TESVESFMRSLAAGRTKVVEHGHALILGTSEA 1223276

Query: 72      VVNVASDCRFTDKSYQTLRE 91
                  V +      K YQ + E
Sbjct: 1223275 TPRVVTQLALMRKRYQKVNE 1223216


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.325    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,637,469
Number of Sequences: 1185
Number of extensions: 151499
Number of successful extensions: 630
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 35
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 514
Number of HSP's gapped (non-prelim): 261
length of query: 209
length of database: 18,886,859
effective HSP length: 99
effective length of query: 110
effective length of database: 18,769,544
effective search space: 2064649840
effective search space used: 2064649840
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000086_1.0 # Protein # Glutathione peroxidase 4 (GPx4) #
Drosophila melanogaster # Complete
         (238 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_34                                                            78   8e-15
scaffold_6                                                             78   8e-15
scaffold_8                                                             64   2e-10
scaffold_69                                                            62   5e-10
scaffold_972                                                           57   2e-08
scaffold_2                                                             33   0.40 
scaffold_11                                                            32   0.69 
scaffold_9                                                             30   2.6  
scaffold_26                                                            30   3.4  
scaffold_23                                                            28   7.6  
scaffold_10                                                            28   7.6  
scaffold_19                                                            28   9.9  
scaffold_3                                                             28   9.9  

>scaffold_34
          Length = 430012

 Score = 78.2 bits (191), Expect = 8e-15
 Identities = 55/172 (31%), Positives = 82/172 (47%), Gaps = 11/172 (6%)
 Frame = -1

Query: 78     NAASIYEFTVKDTHGNDVSLEKYKGKVVLVVNIASKCGLTKNNYEKLTDLKEKYGERGLV 137
              +    ++   +D  G +V    ++G+VV V N+AS+ G T ++Y+ L  L  ++  RGL 
Sbjct: 212233 HVGGFHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLA 212054

Query: 138    ILNFPCNQFGSQMPEADGEAMVC-----HLRDSKADIGEVFA-----KVDVNGDNAAPLY 187
              I+ FPCNQF  Q P A+ E          L  +  D   V A     K DV+G    P++
Sbjct: 212053 IVGFPCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVF 211874

Query: 188    KYLKAKQTGTLGSGIKWNF-TKFLVNKEGVPINRYAPTTDPMDIAKDIEKLL 238
               YLK +        I WNF   FLV+++G           P D+A  +E LL
Sbjct: 211873 SYLKREAP----CKISWNFGAYFLVSRDGAV--EAHENAHPRDLAPRVEALL 211736


>scaffold_6
          Length = 2755913

 Score = 78.2 bits (191), Expect = 8e-15
 Identities = 61/173 (35%), Positives = 82/173 (47%), Gaps = 13/173 (7%)
 Frame = +3

Query: 79      AASIYEFTVKDTHGND-VSLEKYKGKVVLVVNIASKCGLTKNNYEKLTDLKEKYGERGLV 137
               AAS  +F V    G   V+L  Y  +  L+ N ASKCG+T         L+ K+G+R L 
Sbjct: 2424444 AASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMTPQ-LGGFQALQAKFGDR-LQ 2424617

Query: 138     ILNFPCNQFGSQMPEADGEAMVCHLRDSKADIGEVFAKVDVNGDNAAPLYKYLKA----- 192
               +L FPC+QF  Q P +  E +     D       +FAK DVNG +A PL+ +LK      
Sbjct: 2424618 VLAFPCDQF-KQEPGSPAE-IAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQ 2424791

Query: 193     -------KQTGTLGSGIKWNFTKFLVNKEGVPINRYAPTTDPMDIAKDIEKLL 238
                         G   + + WNFTK+LV  +G P  RY+    P  I  DI   L
Sbjct: 2424792 AAPVPPWNNKGQAATDVMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAAL 2424947



 Score = 77.0 bits (188), Expect = 2e-14
 Identities = 54/153 (35%), Positives = 77/153 (50%), Gaps = 10/153 (6%)
 Frame = +2

Query: 92      GNDVSLEKYKGKVVLVVNIASKCGLTKNNYEKLTDLKEKYGERGLVILNFPCNQFGSQMP 151
               G  ++  +Y+ +  LVVN AS+CG      E L  L  +Y   GL +L FPC+QFG Q P
Sbjct: 1870724 GAPLTPARYRRRAHLVVNTASRCGFAPQLRE-LQALWARYP--GLAVLAFPCDQFGGQEP 1870894

Query: 152     EADGEAMVCHLRDSKADIG---EVFAKVDVNGDNAAPLYKYLK-------AKQTGTLGSG 201
                + G+     +RD+    G    VF K DVNG  A PL+ YLK           G     
Sbjct: 1870895 GSAGD-----IRDAYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVPNKGLPRDE 1871059

Query: 202     IKWNFTKFLVNKEGVPINRYAPTTDPMDIAKDI 234
               ++WNFTK+L+  +G  + RY+    P  +  D+
Sbjct: 1871060 VQWNFTKWLI-VDGEVLRRYSFDASPKSVLADV 1871155



 Score = 63.5 bits (153), Expect = 2e-10
 Identities = 40/126 (31%), Positives = 67/126 (53%), Gaps = 7/126 (5%)
 Frame = +2

Query: 102     GKVVLVVNIASKCGLTKNNYEKLTDLKEKYGERGLVILNFPCNQFGSQMPEADGE--AMV 159
               G+VVLV N+AS  G T  ++ ++ DL EK+  +    L FP NQFG Q  E D E   M+
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 160     CHLRDSKADIGE--VFAKVDVNGDNAAPLYKYLKAKQTGTLGSGIKWNFTKFLVNKEGV- 216
                H+R     +    +F K + NG+ A+ L+K+L++   G      K   + ++++ + + 
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRS-SLGAPSDDFKGQGSDYVISTKNII 1233778

Query: 217     --PINR 220
                 P+ R
Sbjct: 1233779 WTPVTR 1233796



 Score = 50.8 bits (120), Expect = 1e-06
 Identities = 21/58 (36%), Positives = 37/58 (63%)
 Frame = +1

Query: 181     DNAAPLYKYLKAKQTGTLGSGIKWNFTKFLVNKEGVPINRYAPTTDPMDIAKDIEKLL 238
               D+   ++   ++  + ++G+ + WNF KFL+N+EG P+ RY+P     D+A D+E LL
Sbjct: 1233814 DDFREIWTGKRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALL 1233987



 Score = 29.3 bits (64), Expect = 4.4
 Identities = 18/59 (30%), Positives = 29/59 (49%)
 Frame = +2

Query: 23      PGPVLELSRGQRQCLRLCTIMLPVSCAATPMNAISSAAQHSTAAAIDMSANGDYKNAAS 81
               P P    +R +R   R  T   P + AATP  + ++AA+  TAA    +  G   ++A+
Sbjct: 1596137 PSPSPPSTRARRPSRRRPTASAPTAAAATPRPSAATAARWRTAARSASAPRGRSTSSAA 1596313


>scaffold_8
          Length = 1961717

 Score = 63.9 bits (154), Expect = 2e-10
 Identities = 66/221 (29%), Positives = 93/221 (42%), Gaps = 24/221 (10%)
 Frame = +3

Query: 25      PVLELSRGQRQ-----CLRLCTIMLPVSCAATPMNAI--------SSAAQHSTAAAIDMS 71
               P LE+    R       L L TI   +     P+  +        S+AA  +  AA+  S
Sbjct: 1062234 PALEIQAAMRSHQHTLALALATIATSLDVRPAPLKPVERRSFLGASAAALAAPRAAVAGS 1062413

Query: 72      -----ANGDYKNAASIYEFTVKDTHGNDVSLEKYKGKVVLVVNIASKCGLTKNNYEKLTD 126
                    A      A  +++  V    G    L K++G   LVVNI     +   N+  L  
Sbjct: 1062414 DGVRPAPAAIPVATKLFDLQVP-FKGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRY 1062590

Query: 127     LKEKYGERGLVILNFPCNQFGSQMPEADGEAMVCHLRDSKADIGE-----VFAKVDVNGD 181
               +   Y E+GL +  FP  Q      E D   +V     S+   G      VF K+DV G+
Sbjct: 1062591 VAANYAEKGLRVWAFPTEQ---GYYEPDVSELVRAKAYSQFGFGTYPTAVVFDKIDVIGN 1062761

Query: 182     NAAPLYKYLKAKQTGTLGSG-IKWNFTKFLVNKEGVPINRY 221
                A PLY+YL A  T   G G +  NF KFL++ +G P+ RY
Sbjct: 1062762 TAHPLYRYL-ALATNPNGVGRLTVNFEKFLLDGDGKPLRRY 1062881



 Score = 28.9 bits (63), Expect = 5.8
 Identities = 22/69 (31%), Positives = 32/69 (46%)
 Frame = -3

Query: 128    KEKYGERGLVILNFPCNQFGSQMPEADGEAMVCHLRDSKADIGEVFAKVDVNGDNAAPLY 187
              +E  GER L  L+    + G ++P  DG       RD + D   + A +   GD+AAP  
Sbjct: 662997 REAAGERDLRPLDAAGRRRGRRVPVRDG-------RDPRRDAQRLLACLRARGDDAAPRP 662839

Query: 188    KYLKAKQTG 196
              + L A   G
Sbjct: 662838 RGLLALVRG 662812


>scaffold_69
          Length = 78151

 Score = 62.4 bits (150), Expect = 5e-10
 Identities = 39/117 (33%), Positives = 58/117 (49%)
 Frame = +2

Query: 115   GLTKNNYEKLTDLKEKYGERGLVILNFPCNQFGSQMPEADGEAMVCHLRDSKADIGEVFA 174
             G T  +Y ++ +L ++ G  GL IL FPCNQFG+Q P A  E +    + +      +  
Sbjct: 68687 GKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEP-ATCEVVAEFAKSAYGADFTMME 68863

Query: 175   KVDVNGDNAAPLYKYLKAKQTGTLGSGIKWNFTKFLVNKEGVPINRYAPTTDPMDIA 231
             KV V+G  A P+Y +L+A    T G+   WNF  + V      +  Y P   P  +A
Sbjct: 68864 KVAVHGPRAHPVYNFLRA----TTGATPSWNFGVYFVVSRSGEVKAY-PDVAPAQLA 69019



 Score = 34.7 bits (78), Expect = 0.11
 Identities = 16/39 (41%), Positives = 24/39 (61%)
 Frame = +3

Query: 79    AASIYEFTVKDTHGNDVSLEKYKGKVVLVVNIASKCGLT 117
             A+S Y+   K   G  ++ + +KGKVVL+ N+AS  G T
Sbjct: 68379 ASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKT 68495



 Score = 30.0 bits (66), Expect = 2.6
 Identities = 20/66 (30%), Positives = 33/66 (50%), Gaps = 8/66 (12%)
 Frame = +3

Query: 11    RQALRCYSMRRTPGPVLELSRGQRQC--------LRLCTIMLPVSCAATPMNAISSAAQH 62
             R++ R  S RR PGP    SR +R+C        LR   + LPVS   +  +A++ A + 
Sbjct: 74739 RRSARRASRRRRPGPPPPRSRSRRRCP*CPPCLRLREVRLGLPVSSLISRRSALAGARRR 74918

Query: 63    STAAAI 68
                +++
Sbjct: 74919 PEMSSL 74936


>scaffold_972
          Length = 3742

 Score = 57.4 bits (137), Expect = 2e-08
 Identities = 61/228 (26%), Positives = 101/228 (44%), Gaps = 4/228 (1%)
 Frame = +3

Query: 15   RCYSMRRTPGPVLELSRGQRQCLRLCTIMLPVSCAATPMNAISSAAQHSTAAAIDMSANG 74
            RC  +RR    +   S G  Q  R   +  P++  A    A+++ A   TA A   +   
Sbjct: 3063 RCQHIRRRVPALSTQSIGTSQ--RSSAVHQPMARRAATTLALATLA---TAFAPMPATAR 3227

Query: 75   DYKNAASIYEFTVKDTHGNDVSLEKYKGKVVLVVNIASKCGLTKNNYEKLTDLKEKYGER 134
                 A+ Y+       G++V+  +++GKV   VN+AS  G T+  Y+ +  L +++G+ 
Sbjct: 3228 SAPVRAAFYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD- 3404

Query: 135  GLVILNFPCNQFGSQ---MPEADGEAMVCHLRDSKADIGEVFAKVDVNGDNAAPLYKYLK 191
             L ++ FP  +FG Q     EA  +     ++   A  G +  K D+   +        +
Sbjct: 3405 DLAVIAFPTGEFGGQELPTDEAIAKFAYDRVKFPAAPRGVLLQKADLGRAS-------WQ 3563

Query: 192  AKQTGTLGSGIKWNFT-KFLVNKEGVPINRYAPTTDPMDIAKDIEKLL 238
            A Q         WNF  KFLV+K+G   +  A   D  DIA  ++ LL
Sbjct: 3564 AMQAEAGADAPAWNFKGKFLVSKDGAVTD--ASGVD--DIAAAVKALL 3695


>scaffold_2
          Length = 3465789

 Score = 32.7 bits (73), Expect = 0.40
 Identities = 19/59 (32%), Positives = 34/59 (57%), Gaps = 2/59 (3%)
 Frame = -3

Query: 135    GLVILNFPCNQFGSQMPEAD--GEAMVCHLRDSKADIGEVFAKVDVNGDNAAPLYKYLK 191
              G+ IL FP ++FG +  + +     + C +  + A    + AKV V+G +A P++KYL+
Sbjct: 153499 GVQILAFPSDEFGPKFTDQEFVDFFVSCGVHPNDASF-RLMAKVRVDGPDAHPVFKYLR 153326



 Score = 28.5 bits (62), Expect = 7.6
 Identities = 20/58 (34%), Positives = 28/58 (48%)
 Frame = -2

Query: 10      SRQALRCYSMRRTPGPVLELSRGQRQCLRLCTIMLPVSCAATPMNAISSAAQHSTAAA 67
               +R+  R    RR+PG      R +R+C R C   +  S  A P  A  SA +  +AAA
Sbjct: 3337490 ARRRRRRRRRRRSPGGPGPGGRRRRRCRRTCAGRICRSTRAPPPRAPRSARRGRSAAA 3337317



 Score = 28.5 bits (62), Expect = 7.6
 Identities = 20/58 (34%), Positives = 30/58 (51%), Gaps = 2/58 (3%)
 Frame = +1

Query: 47      SCAATPMNAISSAAQH--STAAAIDMSANGDYKNAASIYEFTVKDTHGNDVSLEKYKG 102
               SCA TP ++ SSAAQ   S+ A +  +A G   +  S    TV  T  +  S +++ G
Sbjct: 2038651 SCAPTPKSSSSSAAQSSPSSTARVRCAATGP-ASIFSTVRSTVTPTSASPASRDRWSG 2038821


>scaffold_11
          Length = 1405779

 Score = 32.0 bits (71), Expect = 0.69
 Identities = 15/32 (46%), Positives = 20/32 (62%)
 Frame = +3

Query: 30     SRGQRQCLRLCTIMLPVSCAATPMNAISSAAQ 61
              SRG R     C+  LPV+CAAT  ++  S+AQ
Sbjct: 163755 SRGSRSSSGTCSARLPVACAATAQSSQRSSAQ 163850


>scaffold_9
          Length = 1602328

 Score = 30.0 bits (66), Expect = 2.6
 Identities = 15/50 (30%), Positives = 25/50 (50%)
 Frame = -3

Query: 28     ELSRGQRQCLRLCTIMLPVSCAATPMNAISSAAQHSTAAAIDMSANGDYK 77
              E SRG   CLRLC ++  V+   TP + + + A     +  + +A   +K
Sbjct: 787469 EASRGMG-CLRLCALLTAVAAFTTPPSELGATALRGVLSTFERAAEVPFK 787323


>scaffold_26
          Length = 549629

 Score = 29.6 bits (65), Expect = 3.4
 Identities = 18/43 (41%), Positives = 21/43 (48%)
 Frame = +1

Query: 38     RLCTIMLPVSCAATPMNAISSAAQHSTAAAIDMSANGDYKNAA 80
              R C I  P S AA P ++ +S A  STAAA   S       AA
Sbjct: 294520 RACEITGPASAAAPPWSSSASRAPSSTAAARPSSTTAPTATAA 294648


>scaffold_23
          Length = 674037

 Score = 28.5 bits (62), Expect = 7.6
 Identities = 25/74 (33%), Positives = 36/74 (48%), Gaps = 9/74 (12%)
 Frame = -2

Query: 49     AATPMNAISSAAQHSTAAAIDMS-ANGDYKNAA--------SIYEFTVKDTHGNDVSLEK 99
              AATP  A   A Q S AAA+D + A GD    A        ++    V D  G D++L +
Sbjct: 641180 AATPTQAAVLATQISWAAAMDAAVAAGDLAGPAAAIAAKLETLAAAVVADAGGGDLALRR 641001

Query: 100    YKGKVVLVVNIASK 113
               K +  L+V +A +
Sbjct: 641000 RK-REQLIVEVAQQ 640962


>scaffold_10
          Length = 1654512

 Score = 28.5 bits (62), Expect = 7.6
 Identities = 18/55 (32%), Positives = 25/55 (45%)
 Frame = -3

Query: 23     PGPVLELSRGQRQCLRLCTIMLPVSCAATPMNAISSAAQHSTAAAIDMSANGDYK 77
              PGP    SR +R   R        +CA     A  SA Q +TA+A+D     D++
Sbjct: 812122 PGPTSGPSRSRRHPPRK*EYREGAACAINSQAASWSAVQAATASAVDRGLGVDFE 811958



 Score = 28.1 bits (61), Expect = 9.9
 Identities = 22/75 (29%), Positives = 32/75 (42%), Gaps = 10/75 (13%)
 Frame = -3

Query: 15      RCYSMR------RTPGPVLELSRGQRQCLRLCTIMLPVSCAATPMNAI----SSAAQHST 64
               RC S R      R+    + L R      R+       SC ATPM++     S++A H  
Sbjct: 1115632 RCTSRRSRSDFARSAARAVALRRAVASSWRVAVCAASASCCATPMSSSSWRRSASATHEL 1115453

Query: 65      AAAIDMSANGDYKNA 79
                AA+  SA+   + A
Sbjct: 1115452 VAALVASASAAGRGA 1115408


>scaffold_19
          Length = 762245

 Score = 28.1 bits (61), Expect = 9.9
 Identities = 27/109 (24%), Positives = 47/109 (43%), Gaps = 5/109 (4%)
 Frame = -2

Query: 21     RTPGPVLELSRGQRQCLRLCTIMLPVSCAATPMNAISSAAQHSTAAAIDMSANGDYKNAA 80
              R PGP   L R  R+C    +  +  + AA+P  A S+  +   A     +A    + AA
Sbjct: 273769 RAPGPRATLLRAARRC--AVSAKMSGAAAASPGVASSATWKTEPARPSSSTAKNAKRVAA 273596

Query: 81     SIYEF-TVKDTHGNDVSLEKYKGKV----VLVVNIASKCGLTKNNYEKL 124
              ++ E  + +  HG D S+ +         +   ++ SK   +KN   +L
Sbjct: 273595 ALRESPSPRIVHGVDGSMRRCSASAGHASLTTRSVGSKARASKNTIARL 273449


>scaffold_3
          Length = 3001400

 Score = 28.1 bits (61), Expect = 9.9
 Identities = 25/77 (32%), Positives = 38/77 (49%), Gaps = 11/77 (14%)
 Frame = +3

Query: 16      CYSMRRTPGPVLEL----SRGQRQCLRLCTIMLPV---SCA----ATPMNAISSAAQHST 64
               C ++   P   LEL     RG+R+C R+     P    +C     AT  +A   AA+HS+
Sbjct: 1118910 CGNIAH*PAARLELIKTSPRGRRRCGRIERRRRPACLRTCRPCPRATSRSARDRAARHSS 1119089

Query: 65      AAAIDMSANGDYKNAAS 81
               AAA + S +  +  +AS
Sbjct: 1119090 AAAANRSPHARWSPSAS 1119140


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.318    0.133    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,651,399
Number of Sequences: 1185
Number of extensions: 230548
Number of successful extensions: 2065
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 34
Number of HSP's that attempted gapping in prelim test: 800
Number of HSP's gapped (non-prelim): 1655
length of query: 238
length of database: 18,886,859
effective HSP length: 101
effective length of query: 137
effective length of database: 18,767,174
effective search space: 2571102838
effective search space used: 2571102838
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000082_1.0 # Protein # Glutathione peroxidase 1 (GPx1) #
Caenorhabditis elegans # Complete
         (163 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             93   1e-19
scaffold_34                                                            91   5e-19
scaffold_972                                                           72   4e-13
scaffold_69                                                            71   5e-13
scaffold_8                                                             59   2e-09
scaffold_2                                                             37   0.014

>scaffold_6
          Length = 2755913

 Score = 93.2 bits (230), Expect = 1e-19
 Identities = 57/164 (34%), Positives = 92/164 (56%), Gaps = 9/164 (5%)
 Frame = +2

Query: 1       MSSVYDFN-VKNANGDDVSLSDYKGKVLIIVNVASQCGLTNKNYTQLKELLDVYKK-DGL 58
               + + +DF  V    G  ++ + Y+ +  ++VN AS+CG       QL+EL  ++ +  GL
Sbjct: 1870682 LRTYWDFELVPLGGGAPLTPARYRRRAHLVVNTASRCGFA----PQLRELQALWARYPGL 1870849

Query: 59      EVLAFPCNQFAGQEPSCEIDIQAFVADKFKFEPTLFQKIDVNGDKQSPLFKFLKNEKG-- 116
                VLAFPC+QF GQEP    DI+    D++  +  +F+K DVNG +  PLF +LK      
Sbjct: 1870850 AVLAFPCDQFGGQEPGSAGDIRD-AYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQ 1871026

Query: 117     -----GFMFDAIKWNFTKFLVGRDGKIIKRFGPTTDPKDMEKDI 155
                    G   D ++WNFTK+L+  DG++++R+     PK +  D+
Sbjct: 1871027 PVPNKGLPRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADV 1871155



 Score = 93.2 bits (230), Expect = 1e-19
 Identities = 73/174 (41%), Positives = 88/174 (50%), Gaps = 16/174 (9%)
 Frame = +3

Query: 2       SSVYDFNVKNANGDD-VSLSDYKGKVLIIVNVASQCGLTNKNYTQLKELLDVYKK--DGL 58
               +S  DF V    G   V+LS Y  +  +I N AS+CG+T     QL     +  K  D L
Sbjct: 2424447 ASYSDFAVAPLGGGGAVTLSTYAARAHLIFNSASKCGMT----PQLGGFQALQAKFGDRL 2424614

Query: 59      EVLAFPCNQFAGQEPSCEIDIQAFVADKFKFEPTLFQKIDVNGDKQSPLFKFLK------ 112
               +VLAFPC+QF  QEP    +I AF ADKF     LF K DVNG    PLF FLK      
Sbjct: 2424615 QVLAFPCDQFK-QEPGSPAEIAAFYADKFGVTFPLFAKGDVNGPDAHPLFAFLKEATKDQ 2424791

Query: 113     -------NEKGGFMFDAIKWNFTKFLVGRDGKIIKRFGPTTDPKDMEKDIKEAL 159
                      N KG    D + WNFTK+LV  DG   KR+     P+ +E DI  AL
Sbjct: 2424792 AAPVPPWNNKGQAATD-VMWNFTKWLV-VDGTPTKRYSYDVKPEAIEADIAAAL 2424947



 Score = 48.5 bits (114), Expect = 3e-06
 Identities = 33/98 (33%), Positives = 52/98 (53%), Gaps = 5/98 (5%)
 Frame = +2

Query: 24      GKVLIIVNVASQCGLTNKNYTQLKELLDVYKKDGLEVLAFPCNQFAGQEPSCE---IDIQ 80
               G+V+++ NVAS  G T +++TQ+ +L + +       L FP NQF  Q    +   + + 
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKF-AGKFTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 81      AFVADKFKFEPT--LFQKIDVNGDKQSPLFKFLKNEKG 116
               A V     F P   +F K + NG+  S LFKFL++  G
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLG 1233715



 Score = 36.2 bits (82), Expect = 0.018
 Identities = 14/37 (37%), Positives = 23/37 (62%)
 Frame = +1

Query: 123     IKWNFTKFLVGRDGKIIKRFGPTTDPKDMEKDIKEAL 159
               + WNF KFL+ ++GK +KR+ P     D+  D++  L
Sbjct: 1233877 LAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALL 1233987


>scaffold_34
          Length = 430012

 Score = 91.3 bits (225), Expect = 5e-19
 Identities = 56/168 (33%), Positives = 86/168 (51%), Gaps = 12/168 (7%)
 Frame = -1

Query: 5      YDFNVKNANGDDVSLSDYKGKVLIIVNVASQCGLTNKNYTQLKELLDVYKKDGLEVLAFP 64
              +D   ++ +G +V  S ++G+V+ + N+AS+ G TN +Y  L +L   +   GL ++ FP
Sbjct: 212218 HDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGFP 212039

Query: 65     CNQFAGQEPSCEIDIQAFVADKFKFEP-----------TLFQKIDVNGDKQSPLFKFLKN 113
              CNQF GQEP  E +I+AF   +   +P            L +K DV+G    P+F +LK 
Sbjct: 212038 CNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLKR 211859

Query: 114    EKGGFMFDAIKWNF-TKFLVGRDGKIIKRFGPTTDPKDMEKDIKEALG 160
              E        I WNF   FLV RDG +         P+D+   ++  LG
Sbjct: 211858 EAP----CKISWNFGAYFLVSRDGAV--EAHENAHPRDLAPRVEALLG 211733


>scaffold_972
          Length = 3742

 Score = 71.6 bits (174), Expect = 4e-13
 Identities = 52/147 (35%), Positives = 73/147 (49%), Gaps = 5/147 (3%)
 Frame = +3

Query: 2    SSVYDFNVKNANGDDVSLSDYKGKVLIIVNVASQCGLTNKNYTQLKELLDVYKKDGLEVL 61
            ++ YD      +G +V+ S ++GKV   VNVAS  G T   Y  +K+L D +  D L V+
Sbjct: 3243 AAFYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEF-GDDLAVI 3419

Query: 62   AFPCNQFAGQEPSCEIDIQAFVADKFKF----EPTLFQKIDVNGDKQSPLFKFLKNEKGG 117
            AFP  +F GQE   +  I  F  D+ KF       L QK D+        ++ ++ E G 
Sbjct: 3420 AFPTGEFGGQELPTDEAIAKFAYDRVKFPAAPRGVLLQKADLG----RASWQAMQAEAGA 3587

Query: 118  FMFDAIKWNFT-KFLVGRDGKIIKRFG 143
               DA  WNF  KFLV +DG +    G
Sbjct: 3588 ---DAPAWNFKGKFLVSKDGAVTDASG 3659


>scaffold_69
          Length = 78151

 Score = 71.2 bits (173), Expect = 5e-13
 Identities = 39/104 (37%), Positives = 60/104 (57%), Gaps = 2/104 (1%)
 Frame = +2

Query: 37    GLTNKNYTQLKELLDVYKKDGLEVLAFPCNQFAGQEP-SCEIDIQAFVADKFKFEPTLFQ 95
             G T  +Y+Q+ EL D    +GLE+LAFPCNQF  QEP +CE+ +  F    +  + T+ +
Sbjct: 68687 GKTAAHYSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEV-VAEFAKSAYGADFTMME 68863

Query: 96    KIDVNGDKQSPLFKFLKNEKGGFMFDAIKWNF-TKFLVGRDGKI 138
             K+ V+G +  P++ FL+   G        WNF   F+V R G++
Sbjct: 68864 KVAVHGPRAHPVYNFLRATTGA----TPSWNFGVYFVVSRSGEV 68983



 Score = 35.4 bits (80), Expect = 0.030
 Identities = 17/38 (44%), Positives = 23/38 (60%)
 Frame = +3

Query: 2     SSVYDFNVKNANGDDVSLSDYKGKVLIIVNVASQCGLT 39
             SS YD   K A G  ++   +KGKV+++ NVAS  G T
Sbjct: 68382 SSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKT 68495


>scaffold_8
          Length = 1961717

 Score = 59.3 bits (142), Expect = 2e-09
 Identities = 41/133 (30%), Positives = 65/133 (48%), Gaps = 4/133 (3%)
 Frame = +3

Query: 14      GDDVSLSDYKGKVLIIVNVASQCGLTNKNYTQLKELLDVYKKDGLEVLAFPCNQFAGQEP 73
               G    LS ++G   ++VN+     +  +N+  L+ +   Y + GL V AFP  Q    EP
Sbjct: 1062486 GKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYEP 1062662

Query: 74      SCEIDIQAFVADKFKF--EPT--LFQKIDVNGDKQSPLFKFLKNEKGGFMFDAIKWNFTK 129
                    ++A    +F F   PT  +F KIDV G+   PL+++L           +  NF K
Sbjct: 1062663 DVSELVRAKAYSQFGFGTYPTAVVFDKIDVIGNTAHPLYRYLALATNPNGVGRLTVNFEK 1062842

Query: 130     FLVGRDGKIIKRF 142
               FL+  DGK ++R+
Sbjct: 1062843 FLLDGDGKPLRRY 1062881


>scaffold_2
          Length = 3465789

 Score = 36.6 bits (83), Expect = 0.014
 Identities = 31/115 (26%), Positives = 49/115 (42%), Gaps = 4/115 (3%)
 Frame = -3

Query: 2      SSVYDFNVKNANGDDVSLSDYKGKVLIIVNVASQCGLTNKNYTQLKELLD-VYKKDGLEV 60
              S  Y      A G      D   +  IIVNV        +    LK + D +   DG+++
Sbjct: 153667 SRFYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQI 153488

Query: 61     LAFPCNQFAGQEPSCEIDIQAFVADKFKFEPTLFQ---KIDVNGDKQSPLFKFLK 112
              LAFP ++F  +    E  +  FV+         F+   K+ V+G    P+FK+L+
Sbjct: 153487 LAFPSDEFGPKFTDQEF-VDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLR 153326


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.319    0.139    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,212,782
Number of Sequences: 1185
Number of extensions: 72983
Number of successful extensions: 251
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 176
Number of HSP's gapped (non-prelim): 130
length of query: 163
length of database: 18,886,859
effective HSP length: 96
effective length of query: 67
effective length of database: 18,773,099
effective search space: 1257797633
effective search space used: 1257797633
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000083_1.0 # Protein # Glutathione peroxidase 5 (GPx5) #
Caenorhabditis elegans # Complete
         (119 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_7                                                             28   2.7  
scaffold_6                                                             27   6.1  
scaffold_2                                                             27   6.1  
scaffold_1468                                                          26   8.0  

>scaffold_7
          Length = 2177197

 Score = 27.7 bits (60), Expect = 2.7
 Identities = 18/45 (40%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
 Frame = -3

Query: 6       SASNCSMNISMTTSPWHFSKFQMIS-DARIGLYINSSLFPIFSTV 49
               SA +    IS+TTSP   S    +S ++R G + +SSL P F++V
Sbjct: 1708457 SAGDTHAIISVTTSPPSESMRSFVSVESR*GTWASSSLSPFFASV 1708323


>scaffold_6
          Length = 2755913

 Score = 26.6 bits (57), Expect = 6.1
 Identities = 11/17 (64%), Positives = 14/17 (82%)
 Frame = +3

Query: 93     DSANAGADLWRVACHVA 109
              D A+AGADLWR A ++A
Sbjct: 771015 DLADAGADLWRHASNIA 771065


>scaffold_2
          Length = 3465789

 Score = 26.6 bits (57), Expect = 6.1
 Identities = 10/19 (52%), Positives = 12/19 (63%)
 Frame = +2

Query: 84     IEFAELGHFDSANAGADLW 102
              +E  ELGH DSA+   D W
Sbjct: 962030 LELLELGHIDSADVWTDRW 962086


>scaffold_1468
          Length = 1445

 Score = 26.2 bits (56), Expect = 8.0
 Identities = 16/53 (30%), Positives = 26/53 (49%)
 Frame = +1

Query: 29  ISDARIGLYINSSLFPIFSTVCGQDSINNFPNYYIFRGDENSMRKARTPWASY 81
           +S++RIGL +     P  +T+C        P+   FRG   S     +PWA++
Sbjct: 469 LSESRIGLLLQPQSAPTSTTMCS-------PSRCEFRG--ASRHSCGSPWAAH 600


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.324    0.133    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,716,212
Number of Sequences: 1185
Number of extensions: 102165
Number of successful extensions: 704
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 53
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 491
Number of HSP's gapped (non-prelim): 423
length of query: 119
length of database: 18,886,859
effective HSP length: 90
effective length of query: 29
effective length of database: 18,780,209
effective search space: 544626061
effective search space used: 544626061
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 55 (25.8 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000093_1.0 # Protein # Glutathione peroxidase 1 (GPx1) #
Saccharomyces cerevisiae # Complete
         (167 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                             77   7e-15
scaffold_69                                                            63   2e-10
scaffold_972                                                           55   3e-08
scaffold_34                                                            55   3e-08
scaffold_2                                                             44   1e-04

>scaffold_6
          Length = 2755913

 Score = 77.4 bits (189), Expect = 7e-15
 Identities = 46/136 (33%), Positives = 71/136 (52%), Gaps = 6/136 (4%)
 Frame = +2

Query: 14      GNPFPFNSLRNKVVLIVNVASHCAFTPQXXXXXXXXXXXXSHGLVIVAFPCGQFGNQEFE 73
               G P      R +  L+VN AS C F PQ              GL ++AFPC QFG QE  
Sbjct: 1870724 GAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARYP--GLAVLAFPCDQFGGQEPG 1870897

Query: 74      KDKEINKFCQDKYGVTFPILHKIRCNGQKQDPVYKFLKNSVSGK----SGIKM--IKWNF 127
                  +I     D+YGV FP+  K   NG +  P++ +LK + +G+     G+    ++WNF
Sbjct: 1870898 SAGDIRD-AYDRYGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVPNKGLPRDEVQWNF 1871074

Query: 128     EKFVVDRNGKVVKRFS 143
                K+++  +G+V++R+S
Sbjct: 1871075 TKWLI-VDGEVLRRYS 1871119



 Score = 62.4 bits (150), Expect = 2e-10
 Identities = 44/127 (34%), Positives = 58/127 (45%), Gaps = 11/127 (8%)
 Frame = +3

Query: 28      LIVNVASHCAFTPQXXXXXXXXXXXXSHGLVIVAFPCGQFGNQEFEKDKEINKFCQDKYG 87
               LI N AS C  TPQ               L ++AFPC QF  QE     EI  F  DK+G
Sbjct: 2424528 LIFNSASKCGMTPQLGGFQALQAKFGDR-LQVLAFPCDQF-KQEPGSPAEIAAFYADKFG 2424701

Query: 88      VTFPILHKIRCNGQKQDPVYKFLKNSVSGKS-----------GIKMIKWNFEKFVVDRNG 136
               VTFP+  K   NG    P++ FLK +   ++               + WNF K++V  +G
Sbjct: 2424702 VTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPPWNNKGQAATDVMWNFTKWLV-VDG 2424878

Query: 137     KVVKRFS 143
                  KR+S
Sbjct: 2424879 TPTKRYS 2424899



 Score = 40.8 bits (94), Expect(2) = 2e-08
 Identities = 29/99 (29%), Positives = 42/99 (42%), Gaps = 5/99 (5%)
 Frame = +2

Query: 25      KVVLIVNVASHCAFTPQXXXXXXXXXXXXSHGLVIVAFPCGQFGNQEFEKDKEINKFCQD 84
               +VVL+ NVAS    T +            +     + FP  QFG Q  EKD E+      
Sbjct: 1233428 QVVLVENVASI*GTTVRDFTQMNDLTEKFAGKFTCLGFPTNQFGKQTNEKDWELLPMLAH 1233607

Query: 85      K-----YGVTFPILHKIRCNGQKQDPVYKFLKNSVSGKS 118
                     +   FPI  K   NG+    ++KFL++S+   S
Sbjct: 1233608 VRPGGGFVPNFPIFTKTEANGEGASDLFKFLRSSLGAPS 1233724



 Score = 34.3 bits (77), Expect(2) = 2e-08
 Identities = 15/33 (45%), Positives = 22/33 (66%)
 Frame = +1

Query: 111     KNSVSGKSGIKMIKWNFEKFVVDRNGKVVKRFS 143
               K S   +S    + WNFEKF++++ GK VKR+S
Sbjct: 1233841 KRSSPSRSVGTDLAWNFEKFLINQEGKPVKRYS 1233939


>scaffold_69
          Length = 78151

 Score = 62.8 bits (151), Expect = 2e-10
 Identities = 33/84 (39%), Positives = 47/84 (55%), Gaps = 1/84 (1%)
 Frame = +2

Query: 56    GLVIVAFPCGQFGNQEFEKDKEINKFCQDKYGVTFPILHKIRCNGQKQDPVYKFLKNSVS 115
             GL I+AFPC QFGNQE    + + +F +  YG  F ++ K+  +G +  PVY FL+ +  
Sbjct: 68747 GLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEKVAVHGPRAHPVYNFLRATTG 68926

Query: 116   GKSGIKMIKWNF-EKFVVDRNGKV 138
                      WNF   FVV R+G+V
Sbjct: 68927 ATP-----SWNFGVYFVVSRSGEV 68983


>scaffold_972
          Length = 3742

 Score = 55.5 bits (132), Expect = 3e-08
 Identities = 41/136 (30%), Positives = 62/136 (45%), Gaps = 1/136 (0%)
 Frame = +3

Query: 4    FYSFSPIDENGNPFPFNSLRNKVVLIVNVASHCAFTPQXXXXXXXXXXXXSHGLVIVAFP 63
            FY  + +  +G+   F+    KV   VNVAS    T                 L ++AFP
Sbjct: 3249 FYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGDDLAVIAFP 3428

Query: 64   CGQFGNQEFEKDKEINKFCQDKYGVTFPILHKIRCNGQKQDPVYKFLKNSVSGKSGIKMI 123
             G+FG QE   D+ I KF  D+  V FP   +     QK D + +    ++  ++G    
Sbjct: 3429 TGEFGGQELPTDEAIAKFAYDR--VKFPAAPR-GVLLQKAD-LGRASWQAMQAEAGADAP 3596

Query: 124  KWNFE-KFVVDRNGKV 138
             WNF+ KF+V ++G V
Sbjct: 3597 AWNFKGKFLVSKDGAV 3644


>scaffold_34
          Length = 430012

 Score = 55.5 bits (132), Expect = 3e-08
 Identities = 44/148 (29%), Positives = 62/148 (41%), Gaps = 13/148 (8%)
 Frame = -1

Query: 4      FYSFSPIDENGNPFPFNSLRNKVVLIVNVASHCAFTPQXXXXXXXXXXXXS-HGLVIVAF 62
              F+     D +G    F++ R +VV + N+AS    T              S  GL IV F
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 63     PCGQFGNQEFEKDKEINKFCQDKYGV-----------TFPILHKIRCNGQKQDPVYKFLK 111
              PC QF  QE   + EI  F + +  +            F ++ K   +G   DPV+ +LK
Sbjct: 212041 PCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLK 211862

Query: 112    NSVSGKSGIKMIKWNF-EKFVVDRNGKV 138
                   K     I WNF   F+V R+G V
Sbjct: 211861 REAPCK-----ISWNFGAYFLVSRDGAV 211793


>scaffold_2
          Length = 3465789

 Score = 43.5 bits (101), Expect = 1e-04
 Identities = 31/113 (27%), Positives = 51/113 (45%), Gaps = 5/113 (4%)
 Frame = -3

Query: 4      FYSFSPIDENGNPFPFNSLRNKVVLIVNVASHCAFTPQXXXXXXXXXXXXS--HGLVIVA 61
              FY        G PFPF+ L ++  +IVNV  H     +            S   G+ I+A
Sbjct: 153661 FYKLVAHTARGWPFPFDDLHHRKCIIVNVGIHGPDAREEVAALKRISDDISPVDGVQILA 153482

Query: 62     FPCGQFGNQEFEKDKEINKFCQ---DKYGVTFPILHKIRCNGQKQDPVYKFLK 111
              FP  +FG  +F   + ++ F          +F ++ K+R +G    PV+K+L+
Sbjct: 153481 FPSDEFG-PKFTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLR 153326


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.322    0.139    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,374,663
Number of Sequences: 1185
Number of extensions: 67914
Number of successful extensions: 158
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 118
Number of HSP's gapped (non-prelim): 61
length of query: 167
length of database: 18,886,859
effective HSP length: 96
effective length of query: 71
effective length of database: 18,773,099
effective search space: 1332890029
effective search space used: 1332890029
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000094_1.0 # Protein # Glutathione peroxidase 2 (GPx2) #
Saccharomyces cerevisiae # Complete
         (162 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            102   2e-22
scaffold_34                                                            87   1e-17
scaffold_69                                                            74   8e-14
scaffold_972                                                           59   3e-09
scaffold_8                                                             55   3e-08
scaffold_23                                                            32   0.33 

>scaffold_6
          Length = 2755913

 Score =  102 bits (255), Expect = 2e-22
 Identities = 56/130 (43%), Positives = 79/130 (60%), Gaps = 6/130 (4%)
 Frame = +2

Query: 29      LIVNVASKCGFTPQYKELEELYKKYQDKGFVILGFPCNQFGKQEPGSDEQITEFCQLNYG 88
               L+VN AS+CGF PQ +EL+ L+ +Y   G  +L FPC+QFG QEPGS   I +     YG
Sbjct: 1870766 LVVNTASRCGFAPQLRELQALWARYP--GLAVLAFPCDQFGGQEPGSAGDIRDAYD-RYG 1870936

Query: 89      VTFPIMKKIDVNGSNADSVYNYLKSQKAGL------LGFKGIKWNFEKFLVDSNGKVVQR 142
               V FP+ +K DVNG  A  ++ YLK   AG       L    ++WNF K+L+  +G+V++R
Sbjct: 1870937 VDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVPNKGLPRDEVQWNFTKWLI-VDGEVLRR 1871113

Query: 143     FSSLTKPSSL 152
               +S    P S+
Sbjct: 1871114 YSFDASPKSV 1871143



 Score = 89.0 bits (219), Expect = 2e-18
 Identities = 56/144 (38%), Positives = 81/144 (56%), Gaps = 11/144 (7%)
 Frame = +3

Query: 29      LIVNVASKCGFTPQYKELEELYKKYQDKGFVILGFPCNQFGKQEPGSDEQITEFCQLNYG 88
               LI N ASKCG TPQ    + L  K+ D+   +L FPC+QF KQEPGS  +I  F    +G
Sbjct: 2424528 LIFNSASKCGMTPQLGGFQALQAKFGDR-LQVLAFPCDQF-KQEPGSPAEIAAFYADKFG 2424701

Query: 89      VTFPIMKKIDVNGSNADSVYNYL----KSQKAGLLGFKG-------IKWNFEKFLVDSNG 137
               VTFP+  K DVNG +A  ++ +L    K Q A +  +         + WNF K+LV  +G
Sbjct: 2424702 VTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPPWNNKGQAATDVMWNFTKWLV-VDG 2424878

Query: 138     KVVQRFSSLTKPSSLDQEIQSLLS 161
                  +R+S   KP +++ +I + L+
Sbjct: 2424879 TPTKRYSYDVKPEAIEADIAAALN 2424950



 Score = 54.7 bits (130), Expect = 5e-08
 Identities = 36/95 (37%), Positives = 52/95 (54%), Gaps = 6/95 (6%)
 Frame = +2

Query: 25      GKVVLIVNVASKCGFTPQ-YKELEELYKKYQDKGFVILGFPCNQFGKQEPGSD-EQITEF 82
               G+VVL+ NVAS  G T + + ++ +L +K+  K F  LGFP NQFGKQ    D E +   
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 83      CQLNYG----VTFPIMKKIDVNGSNADSVYNYLKS 113
                 +  G      FPI  K + NG  A  ++ +L+S
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRS 1233706



 Score = 35.0 bits (79), Expect = 0.039
 Identities = 14/37 (37%), Positives = 25/37 (67%)
 Frame = +1

Query: 124     IKWNFEKFLVDSNGKVVQRFSSLTKPSSLDQEIQSLL 160
               + WNFEKFL++  GK V+R+S     + +  ++++LL
Sbjct: 1233877 LAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALL 1233987


>scaffold_34
          Length = 430012

 Score = 86.7 bits (213), Expect = 1e-17
 Identities = 54/168 (32%), Positives = 86/168 (51%), Gaps = 12/168 (7%)
 Frame = -1

Query: 5      FYDLECKDKKGESFKFDQLKGKVVLIVNVASKCGFT-PQYKELEELYKKYQDKGFVILGF 63
              F+DL  +D  G   +F   +G+VV + N+AS+ G T   YK L +L  ++  +G  I+GF
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 64     PCNQFGKQEPGSDEQITEFCQLNYGV-----------TFPIMKKIDVNGSNADSVYNYLK 112
              PCNQF  QEPG++ +I  F +    +            F +M+K DV+G+  D V++YLK
Sbjct: 212041 PCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLK 211862

Query: 113    SQKAGLLGFKGIKWNFEKFLVDSNGKVVQRFSSLTKPSSLDQEIQSLL 160
               +         I WNF  + + S    V+   +   P  L   +++LL
Sbjct: 211861 REAP-----CKISWNFGAYFLVSRDGAVEAHEN-AHPRDLAPRVEALL 211736


>scaffold_69
          Length = 78151

 Score = 73.9 bits (180), Expect = 8e-14
 Identities = 37/101 (36%), Positives = 55/101 (54%)
 Frame = +2

Query: 43    YKELEELYKKYQDKGFVILGFPCNQFGKQEPGSDEQITEFCQLNYGVTFPIMKKIDVNGS 102
             Y ++ EL+ +   +G  IL FPCNQFG QEP + E + EF +  YG  F +M+K+ V+G 
Sbjct: 68705 YSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEKVAVHGP 68884

Query: 103   NADSVYNYLKSQKAGLLGFKGIKWNFEKFLVDSNGKVVQRF 143
              A  VYN+L++            WNF  + V S    V+ +
Sbjct: 68885 RAHPVYNFLRATTGAT-----PSWNFGVYFVVSRSGEVKAY 68992



 Score = 43.5 bits (101), Expect = 1e-04
 Identities = 21/45 (46%), Positives = 29/45 (64%)
 Frame = +3

Query: 1     MTTSFYDLECKDKKGESFKFDQLKGKVVLIVNVASKCGFTPQYKE 45
             + +SFYDL+ K   G +  FD  KGKVVL+ NVAS  G T  +++
Sbjct: 68376 VASSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAHEK 68510


>scaffold_972
          Length = 3742

 Score = 58.5 bits (140), Expect = 3e-09
 Identities = 47/144 (32%), Positives = 69/144 (47%), Gaps = 8/144 (5%)
 Frame = +3

Query: 4    SFYDLECKDKKGESFKFDQLKGKVVLIVNVASKCGFT-PQYKELEELYKKYQDKGFVILG 62
            +FYDL      G    F Q +GKV   VNVAS  G T P YK +++L  ++ D    ++ 
Sbjct: 3246 AFYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGD-DLAVIA 3422

Query: 63   FPCNQFGKQEPGSDEQITEFCQLNYGVTFP------IMKKIDVNGSNADSVYNYLKSQKA 116
            FP  +FG QE  +DE I +F      V FP      +++K D+  ++  ++         
Sbjct: 3423 FPTGEFGGQELPTDEAIAKFAYDR--VKFPAAPRGVLLQKADLGRASWQAM--------Q 3572

Query: 117  GLLGFKGIKWNFE-KFLVDSNGKV 139
               G     WNF+ KFLV  +G V
Sbjct: 3573 AEAGADAPAWNFKGKFLVSKDGAV 3644


>scaffold_8
          Length = 1961717

 Score = 55.5 bits (132), Expect = 3e-08
 Identities = 44/149 (29%), Positives = 71/149 (47%), Gaps = 6/149 (4%)
 Frame = +3

Query: 1       MTTSFYDLECKDKKGESFKFDQLKGKVVLIVNVASKCGFTPQ-YKELEELYKKYQDKGFV 59
               + T  +DL+   K G+     + +G   L+VN+        + +  L  +   Y +KG  
Sbjct: 1062447 VATKLFDLQVPFK-GKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLR 1062623

Query: 60      ILGFPCNQFGKQEPGSDEQITE--FCQLNYGVTFP---IMKKIDVNGSNADSVYNYLKSQ 114
               +  FP  Q G  EP   E +    + Q  +G T+P   +  KIDV G+ A  +Y YL + 
Sbjct: 1062624 VWAFPTEQ-GYYEPDVSELVRAKAYSQFGFG-TYPTAVVFDKIDVIGNTAHPLYRYL-AL 1062794

Query: 115     KAGLLGFKGIKWNFEKFLVDSNGKVVQRF 143
                    G   +  NFEKFL+D +GK ++R+
Sbjct: 1062795 ATNPNGVGRLTVNFEKFLLDGDGKPLRRY 1062881


>scaffold_23
          Length = 674037

 Score = 32.0 bits (71), Expect = 0.33
 Identities = 16/31 (51%), Positives = 20/31 (64%)
 Frame = -1

Query: 4      SFYDLECKDKKGESFKFDQLKGKVVLIVNVA 34
              SF+DL+ +   GE+  F   KGK VL VNVA
Sbjct: 120669 SFFDLKGRTNAGEAVDFATHKGKCVLAVNVA 120577


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.317    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,722,468
Number of Sequences: 1185
Number of extensions: 60512
Number of successful extensions: 172
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 21
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 127
Number of HSP's gapped (non-prelim): 61
length of query: 162
length of database: 18,886,859
effective HSP length: 96
effective length of query: 66
effective length of database: 18,773,099
effective search space: 1239024534
effective search space used: 1239024534
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000095_1.0 # Protein # Glutathione peroxidase 3 (GPx3) #
Saccharomyces cerevisiae # Complete
         (163 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_6                                                            106   1e-23
scaffold_34                                                            85   4e-17
scaffold_69                                                            73   1e-13
scaffold_972                                                           63   1e-10
scaffold_8                                                             60   9e-10
scaffold_2                                                             59   2e-09

>scaffold_6
          Length = 2755913

 Score =  106 bits (265), Expect = 1e-23
 Identities = 66/166 (39%), Positives = 95/166 (57%), Gaps = 8/166 (4%)
 Frame = +2

Query: 5       YKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFTPQYKELEALYKRYKDEGFTIIGFPC 64
               ++L P+   G P    + + +  L+VN AS+CGF PQ +EL+AL+ RY   G  ++ FPC
Sbjct: 1870700 FELVPLGG-GAPLTPARYRRRAHLVVNTASRCGFAPQLRELQALWARY--PGLAVLAFPC 1870870

Query: 65      NQFGHQEPGSDEEIAQFCQLNYGVTFPIMKKIDVNGGNEDPVYKFLKSQKSGM------L 118
               +QFG QEPGS  +I       YGV FP+ +K DVNG    P++ +LK   +G       L
Sbjct: 1870871 DQFGGQEPGSAGDIRDAYD-RYGVDFPVFEKSDVNGPRAHPLFTYLKKATAGQPVPNKGL 1871047

Query: 119     GLRGIKWNFEKFLVDKKGKVYERYSSLTKPSS-LSETIEEL-LKEV 162
                   ++WNF K+L+   G+V  RYS    P S L++ +  L LKEV
Sbjct: 1871048 PRDEVQWNFTKWLI-VDGEVLRRYSFDASPKSVLADVVYTLKLKEV 1871182



 Score = 88.6 bits (218), Expect = 3e-18
 Identities = 56/143 (39%), Positives = 72/143 (50%), Gaps = 11/143 (7%)
 Frame = +3

Query: 28      LIVNVASKCGFTPQYKELEALYKRYKDEGFTIIGFPCNQFGHQEPGSDEEIAQFCQLNYG 87
               LI N ASKCG TPQ    +AL  ++ D    ++ FPC+QF  QEPGS  EIA F    +G
Sbjct: 2424528 LIFNSASKCGMTPQLGGFQALQAKFGDR-LQVLAFPCDQF-KQEPGSPAEIAAFYADKFG 2424701

Query: 88      VTFPIMKKIDVNGGNEDPVYKFLKSQKSGML-----------GLRGIKWNFEKFLVDKKG 136
               VTFP+  K DVNG +  P++ FLK                      + WNF K+LV   G
Sbjct: 2424702 VTFPLFAKGDVNGPDAHPLFAFLKEATKDQAAPVPPWNNKGQAATDVMWNFTKWLV-VDG 2424878

Query: 137     KVYERYSSLTKPSSLSETIEELL 159
                  +RYS   KP ++   I   L
Sbjct: 2424879 TPTKRYSYDVKPEAIEADIAAAL 2424947



 Score = 50.1 bits (118), Expect = 1e-06
 Identities = 33/95 (34%), Positives = 50/95 (52%), Gaps = 6/95 (6%)
 Frame = +2

Query: 24      GKVVLIVNVASKCGFTPQ-YKELEALYKRYKDEGFTIIGFPCNQFGHQEPGSDEEIAQFC 82
               G+VVL+ NVAS  G T + + ++  L +++  + FT +GFP NQFG Q    D E+    
Sbjct: 1233425 GQVVLVENVASI*GTTVRDFTQMNDLTEKFAGK-FTCLGFPTNQFGKQTNEKDWELLPML 1233601

Query: 83      QL-----NYGVTFPIMKKIDVNGGNEDPVYKFLKS 112
                       +   FPI  K + NG     ++KFL+S
Sbjct: 1233602 AHVRPGGGFVPNFPIFTKTEANGEGASDLFKFLRS 1233706



 Score = 36.2 bits (82), Expect = 0.018
 Identities = 15/38 (39%), Positives = 26/38 (68%)
 Frame = +1

Query: 123     IKWNFEKFLVDKKGKVYERYSSLTKPSSLSETIEELLK 160
               + WNFEKFL++++GK  +RYS     + ++  +E LL+
Sbjct: 1233877 LAWNFEKFLINQEGKPVKRYSPGFLTADVAADVEALLE 1233990


>scaffold_34
          Length = 430012

 Score = 84.7 bits (208), Expect = 4e-17
 Identities = 59/173 (34%), Positives = 84/173 (48%), Gaps = 13/173 (7%)
 Frame = -1

Query: 4      FYKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFT-PQYKELEALYKRYKDEGFTIIGF 62
              F+ L   D  G    F   +G+VV + N+AS+ G T   YK L  L  ++   G  I+GF
Sbjct: 212221 FHDLMSEDIDGAEVRFSAFRGQVVCVTNLASR*GKTNSHYKGLAKLGAQFSGRGLAIVGF 212042

Query: 63     PCNQFGHQEPGSDEEIAQFCQLNYGV-----------TFPIMKKIDVNGGNEDPVYKFLK 111
              PCNQF  QEPG++ EI  F +    +            F +M+K DV+G   DPV+ +LK
Sbjct: 212041 PCNQFMGQEPGAESEIKAFARSRGFLDPALGDASAVGAFNLMRKCDVSGAAVDPVFSYLK 211862

Query: 112    SQKSGMLGLRGIKWNF-EKFLVDKKGKVYERYSSLTKPSSLSETIEELLKEVE 163
               +         I WNF   FLV + G V    ++   P  L+  +E LL  V+
Sbjct: 211861 REAPCK-----ISWNFGAYFLVSRDGAVEAHENA--HPRDLAPRVEALLGAVD 211724


>scaffold_69
          Length = 78151

 Score = 73.2 bits (178), Expect = 1e-13
 Identities = 37/98 (37%), Positives = 55/98 (56%), Gaps = 1/98 (1%)
 Frame = +2

Query: 42    YKELEALYKRYKDEGFTIIGFPCNQFGHQEPGSDEEIAQFCQLNYGVTFPIMKKIDVNGG 101
             Y ++  L+     EG  I+ FPCNQFG+QEP + E +A+F +  YG  F +M+K+ V+G 
Sbjct: 68705 YSQMVELHDELGHEGLEILAFPCNQFGNQEPATCEVVAEFAKSAYGADFTMMEKVAVHGP 68884

Query: 102   NEDPVYKFLKSQKSGMLGLRGIKWNF-EKFLVDKKGKV 138
                PVY FL++            WNF   F+V + G+V
Sbjct: 68885 RAHPVYNFLRATTGAT-----PSWNFGVYFVVSRSGEV 68983



 Score = 35.8 bits (81), Expect = 0.023
 Identities = 19/46 (41%), Positives = 25/46 (54%)
 Frame = +3

Query: 2     SEFYKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFTPQYKELEA 47
             S FY L      G    FD  KGKVVL+ NVAS  G T  +++ ++
Sbjct: 68382 SSFYDLKAKTAAGATLTFDAFKGKVVLLTNVASA*GKTAAHEKSDS 68519


>scaffold_972
          Length = 3742

 Score = 63.2 bits (152), Expect = 1e-10
 Identities = 51/143 (35%), Positives = 72/143 (50%), Gaps = 8/143 (5%)
 Frame = +3

Query: 4    FYKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFT-PQYKELEALYKRYKDEGFTIIGF 62
            FY LA V   G    F Q +GKV   VNVAS  G T P YK ++ L   + D+   +I F
Sbjct: 3249 FYDLADVANDGSEVAFSQFEGKVCYAVNVASA*GATRPGYKLMKQLSDEFGDD-LAVIAF 3425

Query: 63   PCNQFGHQEPGSDEEIAQFCQLNYGVTFP------IMKKIDVNGGNEDPVYKFLKSQKSG 116
            P  +FG QE  +DE IA+F      V FP      +++K D+   +    ++ ++++   
Sbjct: 3426 PTGEFGGQELPTDEAIAKFAYDR--VKFPAAPRGVLLQKADLGRAS----WQAMQAE--- 3578

Query: 117  MLGLRGIKWNFE-KFLVDKKGKV 138
              G     WNF+ KFLV K G V
Sbjct: 3579 -AGADAPAWNFKGKFLVSKDGAV 3644


>scaffold_8
          Length = 1961717

 Score = 60.5 bits (145), Expect = 9e-10
 Identities = 42/136 (30%), Positives = 67/136 (49%), Gaps = 6/136 (4%)
 Frame = +3

Query: 13      KGQPFPFDQLKGKVVLIVNVASKCGFTPQ-YKELEALYKRYKDEGFTIIGFPCNQFGHQE 71
               KG+P P  + +G   L+VN+        + +  L  +   Y ++G  +  FP  Q G+ E
Sbjct: 1062483 KGKPQPLSKFRGSAHLVVNIKMDDPVAGENFNALRYVAANYAEKGLRVWAFPTEQ-GYYE 1062659

Query: 72      PGSDEEIAQ--FCQLNYGVTFP---IMKKIDVNGGNEDPVYKFLKSQKSGMLGLRGIKWN 126
               P   E +    + Q  +G T+P   +  KIDV G    P+Y++L +  +   G+  +  N
Sbjct: 1062660 PDVSELVRAKAYSQFGFG-TYPTAVVFDKIDVIGNTAHPLYRYL-ALATNPNGVGRLTVN 1062833

Query: 127     FEKFLVDKKGKVYERY 142
               FEKFL+D  GK   RY
Sbjct: 1062834 FEKFLLDGDGKPLRRY 1062881


>scaffold_2
          Length = 3465789

 Score = 59.3 bits (142), Expect = 2e-09
 Identities = 41/119 (34%), Positives = 62/119 (52%), Gaps = 9/119 (7%)
 Frame = -3

Query: 2      SEFYKLAPVDKKGQPFPFDQLKGKVVLIVNVASKCGFTPQYKELEALYKRYKD-----EG 56
              S FYKL     +G PFPFD L  +  +IVNV       P  +E  A  KR  D     +G
Sbjct: 153667 SRFYKLVAHTARGWPFPFDDLHHRKCIIVNVGI---HGPDAREEVAALKRISDDISPVDG 153497

Query: 57     FTIIGFPCNQFGHQEPGSDEEIAQF---CQLN-YGVTFPIMKKIDVNGGNEDPVYKFLK 111
                I+ FP ++FG +   +D+E   F   C ++    +F +M K+ V+G +  PV+K+L+
Sbjct: 153496 VQILAFPSDEFGPK--FTDQEFVDFFVSCGVHPNDASFRLMAKVRVDGPDAHPVFKYLR 153326


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.317    0.139    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,232,887
Number of Sequences: 1185
Number of extensions: 87600
Number of successful extensions: 230
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 170
Number of HSP's gapped (non-prelim): 108
length of query: 163
length of database: 18,886,859
effective HSP length: 96
effective length of query: 67
effective length of database: 18,773,099
effective search space: 1257797633
effective search space used: 1257797633
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
