TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000001_1.0 # Protein # Iodothyronine deiodinase 1 (DI1) #
Homo sapiens # Complete
         (249 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_8                                                             54   2e-07
scaffold_2                                                             33   0.33 
scaffold_18                                                            30   2.8  

>scaffold_8
          Length = 1961717

 Score = 53.9 bits (128), Expect = 2e-07
 Identities = 45/167 (26%), Positives = 79/167 (47%), Gaps = 10/167 (5%)
 Frame = -1

Query: 88      LGGLAPNCPVVRLSGQRCNIWEFMQGNRPLVLNFGSCTXPSFMFKFDQFKRLIEDFSSIA 147
               +G  AP+  + RL G + ++       +PLV+  GS + P F     +   L  +    A
Sbjct: 1757606 VGDAAPDVALWRLDGSKTSLLAGRDAAKPLVVVAGSVS*PPFRRVGPELDALARECPG-A 1757430

Query: 148     DFLVIYIEEAHASDGWAFKN------NMDIRNHQNLQDRLQAAHLLLA-RSPQCPVVVDT 200
               DF  +Y+ EAHA+D W  ++       + +   + L DR+  A    A      PVVVD 
Sbjct: 1757429 DFTFVYVAEAHAADEWPLRSARFAAAPVVVDQPRTLADRVALARRYAADYGIASPVVVDD 1757250

Query: 201     MQNQS-SQLYAALPERLYIIQEGRILYKGKSGPWNY--NPEEVRAVL 244
                 +++  + YA  P RLY+++   + +  +    +Y  +  E+RA+L
Sbjct: 1757249 PTDEAFERAYAPWPLRLYVVRGAALAWIAEPDGASYERSVAELRAML 1757109


>scaffold_2
          Length = 3465789

 Score = 33.1 bits (74), Expect = 0.33
 Identities = 27/94 (28%), Positives = 44/94 (46%), Gaps = 3/94 (3%)
 Frame = -3

Query: 153     YIEEAHASDGWAFKNNMDIRNHQNLQDRLQAAHLLLARSPQCPVVVDTMQNQSSQLYAAL 212
               + ++A AS G+AF         ++ Q +L  + L    SP+    V + +   + L  A 
Sbjct: 1482424 FFQDASASAGFAFPQ-------RDGQRKLWQSRLAKQWSPREANDVSSSEIAMAALVDAE 1482266

Query: 213     PERLYIIQEGRILYKGKSGP---WNYNPEEVRAV 243
               PERL++  EGR  ++    P   W Y P   RA+
Sbjct: 1482265 PERLHVCYEGRETFEVAIDPPKSWRYGPVVGRAL 1482164


>scaffold_18
          Length = 809113

 Score = 30.0 bits (66), Expect = 2.8
 Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 7/68 (10%)
 Frame = -2

Query: 174    HQNLQDRLQAAH-----LLLARSPQCPVVVDTMQNQSSQLYAALPERLYIIQ--EGRILY 226
              H ++ DR  AA      L L  +    +V+D+M +     Y   P R+Y+++   G I Y
Sbjct: 372183 HASIDDRKAAAEKALSLLRLKIAGPVTMVLDSMDDALEAAYETRPWRVYVVEVATGAIAY 372004

Query: 227    KGKSGPWN 234
                  GP N
Sbjct: 372003 ASGPGPMN 371980


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.324    0.138    0.448 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,441,209
Number of Sequences: 1185
Number of extensions: 202132
Number of successful extensions: 1022
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 33
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 837
Number of HSP's gapped (non-prelim): 382
length of query: 249
length of database: 18,886,859
effective HSP length: 101
effective length of query: 148
effective length of database: 18,767,174
effective search space: 2777541752
effective search space used: 2777541752
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 61 (28.1 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000002_1.0 # Protein # Iodothyronine deiodinase 2 (DI2) #
Homo sapiens # Complete
         (273 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_8                                                             47   3e-05
scaffold_31                                                            32   1.1  
scaffold_7                                                             30   4.2  
scaffold_15                                                            29   7.2  
scaffold_9                                                             28   9.5  
scaffold_5                                                             28   9.5  

>scaffold_8
          Length = 1961717

 Score = 46.6 bits (109), Expect = 3e-05
 Identities = 41/144 (28%), Positives = 62/144 (43%), Gaps = 2/144 (1%)
 Frame = -1

Query: 108     EGATCHLLDFASPERPLVVNFGSATXPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPS 167
               +G+   LL      +PLVV  GS + PPF    P    L  E    ADF  VY+ EAH +
Sbjct: 1757567 DGSKTSLLAGRDAAKPLVVVAGSVS*PPFRRVGPELDALARECPG-ADFTFVYVAEAHAA 1757391

Query: 168     DGWAIPGD--SSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGV 225
               D W +     ++    V + +   DR A A++    + +     VV D  D     AY  
Sbjct: 1757390 DEWPLRSARFAAAPVVVDQPRTLADRVALARRYAADYGIASPV-VVDDPTDEAFERAYAP 1757214

Query: 226     AFERVCIVQRQKIAYLGGKGPFSY 249
                  R+ +V+   +A++      SY
Sbjct: 1757213 WPLRLYVVRGAALAWIAEPDGASY 1757142


>scaffold_31
          Length = 470201

 Score = 31.6 bits (70), Expect = 1.1
 Identities = 30/94 (31%), Positives = 48/94 (51%), Gaps = 7/94 (7%)
 Frame = +1

Query: 161    IDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMD--NN 218
              ++E H  DG A PGD      V++     +R    + ++     PP+ RV A R +  + 
Sbjct: 426940 VEERH-GDGLA-PGD------VRRQHRARERRERRRLVVAEEVHPPRPRVRAPRQELPDR 427095

Query: 219    ANIAYG--VAFERVCIVQR---QKIAYLGGKGPF 247
                +A G  +  E+V +V R   ++IAYLGG+G F
Sbjct: 427096 GRVALGGPLEQEQVEVVLRGAHERIAYLGGRGGF 427197


>scaffold_7
          Length = 2177197

 Score = 29.6 bits (65), Expect = 4.2
 Identities = 16/37 (43%), Positives = 18/37 (48%)
 Frame = +2

Query: 42     SRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQVKLG 78
              S S STR  W R  +S   R VWK   L  +K   LG
Sbjct: 670094 SGSSSTRSPWARRTSSTRSRSVWKESELPNFKGSDLG 670204


>scaffold_15
          Length = 987846

 Score = 28.9 bits (63), Expect = 7.2
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +2

Query: 43     RSKSTRGEWRRMLTSEGLRCVWKSFLLDAY 72
              RS+  RGE  R+  S G  C++K  +L  Y
Sbjct: 788369 RSEGARGESNRLRNSRGCACIYKQSILRRY 788458


>scaffold_9
          Length = 1602328

 Score = 28.5 bits (62), Expect = 9.5
 Identities = 26/82 (31%), Positives = 40/82 (48%), Gaps = 2/82 (2%)
 Frame = -3

Query: 126    VNFGSATXPPFTSQ-LPAFRKLVEEFSSVADFLLVY-IDEAHPSDGWAIPGDSSLSFEVK 183
              VN+G AT PP ++Q LP  R++   F++ A  L  Y +DE     G A+  D  ++ +  
Sbjct: 292583 VNYGWATVPPQSAQTLP--REIT--FNAAARLLQQYPVDEIAKLRGAAVVVDDDVTVDGA 292416

Query: 184    KHQNQEDRCAAAQQLLERFSLP 205
                 +       A +LL  F LP
Sbjct: 292415 HAIDVPGNVTKASELLATFELP 292350


>scaffold_5
          Length = 3000003

 Score = 28.5 bits (62), Expect = 9.5
 Identities = 11/25 (44%), Positives = 14/25 (56%)
 Frame = +3

Query: 42      SRSKSTRGEWRRMLTSEGLRCVWKS 66
               +R+ S    WRR  TS   RC W+S
Sbjct: 1338159 ARTPSQSRRWRRSRTSSSSRCAWRS 1338233


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.320    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,689,454
Number of Sequences: 1185
Number of extensions: 290375
Number of successful extensions: 1455
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 82
Number of HSP's successfully gapped in prelim test: 29
Number of HSP's that attempted gapping in prelim test: 770
Number of HSP's gapped (non-prelim): 1098
length of query: 273
length of database: 18,886,859
effective HSP length: 102
effective length of query: 171
effective length of database: 18,765,989
effective search space: 3208984119
effective search space used: 3208984119
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= SPP00000003_1.0 # Protein # Iodothyronine deiodinase 3 (DI3) #
Homo sapiens # Complete
         (278 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_8                                                             55   1e-07
scaffold_18                                                            33   0.39 
scaffold_3                                                             31   1.5  
scaffold_4                                                             30   3.3  
scaffold_877                                                           30   4.3  
scaffold_29                                                            30   4.3  
scaffold_23                                                            30   4.3  
scaffold_7                                                             30   4.3  
scaffold_110                                                           28   9.7  
scaffold_9                                                             28   9.7  
scaffold_2                                                             28   9.7  

>scaffold_8
          Length = 1961717

 Score = 54.7 bits (130), Expect = 1e-07
 Identities = 49/169 (28%), Positives = 76/169 (44%), Gaps = 11/169 (6%)
 Frame = -1

Query: 106     GGPAPNSEVVLPDGFQSQHILDYAQGNRPLVLNFGSCTXPPFMARMSAFQRLVTKYQRDV 165
               G  AP+  +   DG ++  +L      +PLV+  GS + PPF  R+      + +     
Sbjct: 1757603 GDAAPDVALWRLDGSKTS-LLAGRDAAKPLVVVAGSVS*PPFR-RVGPELDALARECPGA 1757430

Query: 166     DFLIIYIEEAHPSDGWVTTD-----SPYIIPQHRSLEDRVSAAR--VLQQGAPGCALVLD 218
               DF  +Y+ EAH +D W         +P ++ Q R+L DRV+ AR      G     +V D
Sbjct: 1757429 DFTFVYVAEAHAADEWPLRSARFAAAPVVVDQPRTLADRVALARRYAADYGIASPVVVDD 1757250

Query: 219     TMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDG----YQVSELRTWL 263
                   +   AY  +  RLYV++   + +     PDG      V+ELR  L
Sbjct: 1757249 PTDEAFERAYAPWPLRLYVVRGAALAWIA--EPDGASYERSVAELRAML 1757109


>scaffold_18
          Length = 809113

 Score = 33.1 bits (74), Expect = 0.39
 Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 7/66 (10%)
 Frame = -2

Query: 193    HRSLEDRVSAAR----VLQQGAPG-CALVLDTMANSSSSAYGAYFERLYVIQ--SGTIMY 245
              H S++DR +AA     +L+    G   +VLD+M ++  +AY     R+YV++  +G I Y
Sbjct: 372183 HASIDDRKAAAEKALSLLRLKIAGPVTMVLDSMDDALEAAYETRPWRVYVVEVATGAIAY 372004

Query: 246    QGGRGP 251
                G GP
Sbjct: 372003 ASGPGP 371986


>scaffold_3
          Length = 3001400

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 15/52 (28%), Positives = 24/52 (46%)
 Frame = +1

Query: 23      FPRFLGTAFMLWLLDFLCIRKHFLGRRRRGQPEPEVELNSEGEEVPPDDPPI 74
               +P   G A +   LDF C R+    +    +  PE+    + +E PP  PP+
Sbjct: 2052763 WPLLAGAALLCLCLDFACRRRPCRTKEPPAEEPPEMVSYLDSDEPPPPLPPV 2052918



 Score = 29.3 bits (64), Expect = 5.7
 Identities = 15/35 (42%), Positives = 19/35 (54%)
 Frame = +2

Query: 187     PYIIPQHRSLEDRVSAARVLQQGAPGCALVLDTMA 221
               P  +P+ R L+DRV+A R    G P   LVL   A
Sbjct: 2160503 PPRLPRERRLQDRVAARRAQHHGVPARDLVLAAAA 2160607



 Score = 28.9 bits (63), Expect = 7.4
 Identities = 12/28 (42%), Positives = 15/28 (53%)
 Frame = -1

Query: 248     GRGPDGYQVSELRTWLERYDEQLHGARP 275
               GR P    V  +R+W  R +  LHG RP
Sbjct: 1434881 GRPPPPPNVQPVRSWYTRLERALHGRRP 1434798



 Score = 28.5 bits (62), Expect = 9.7
 Identities = 22/81 (27%), Positives = 32/81 (39%), Gaps = 3/81 (3%)
 Frame = +1

Query: 145     PPFMARMSAFQRLVTKYQRDVDFLIIYIEEAHPSDGW---VTTDSPYIIPQHRSLEDRVS 201
               PP     +A  R  T  Q  V    + +E +  +  W     T  P I P+H   + R S
Sbjct: 1684690 PPPTTTPTATSRFATSAQSTV----LSVEHSVQTPLWPCTTKTSRPVIAPKHCGSDSRQS 1684857

Query: 202     AARVLQQGAPGCALVLDTMAN 222
                 R +  G P    +  TMA+
Sbjct: 1684858 TRRPMHMGVPVKVCMRKTMAS 1684920


>scaffold_4
          Length = 3024044

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 23/67 (34%), Positives = 26/67 (38%)
 Frame = -2

Query: 48      RRRRGQPEPEVELNSEGEEVPPDDPPICVSDDNRLCTLASLKAVWHGQKLDFFKQAHEGG 107
               RRRR +P P       G E PP  PP C+S   R    A    V     +   K  HEG 
Sbjct: 2134468 RRRRRRPPPP----PRGAEGPPPPPPACLSGTERDRRSALRYLVLSAVLVITQKPRHEGD 2134301

Query: 108     PAPNSEV 114
                    EV
Sbjct: 2134300 GGAAGEV 2134280



 Score = 28.5 bits (62), Expect = 9.7
 Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
 Frame = -2

Query: 248     GRGPDGYQVSELRTWLERYDEQL---HGARPRR 277
               GRG  G ++ ELR   ER D++L   HG R RR
Sbjct: 2265250 GRGGHGRELLELRVAEERVDDELDVGHGERHRR 2265152


>scaffold_877
          Length = 4090

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 27/84 (32%), Positives = 34/84 (40%)
 Frame = -2

Query: 194  RSLEDRVSAARVLQQGAPGCALVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDG 253
            R  E R +A RVLQ G+PG                G   ERL+ ++SG      GRG DG
Sbjct: 3516 RRREGRAAARRVLQHGSPG---------------PGGRRERLFALRSGARREARGRG-DG 3385

Query: 254  YQVSELRTWLERYDEQLHGARPRR 277
             +    R  L     +   A P R
Sbjct: 3384 RRHRRRRRLLHELRRRGPRAAPPR 3313


>scaffold_29
          Length = 462849

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +1

Query: 44    HFLGRRRRGQPEPEVELNSEGEEVPPDDPP 73
             HF G+  RG+P  +   + EG + PP  PP
Sbjct: 34117 HFRGQAARGRPHAQRLQHPEGVDAPPRPPP 34206



 Score = 29.6 bits (65), Expect = 4.3
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = +3

Query: 44    HFLGRRRRGQPEPEVELNSEGEEVPPDDPP 73
             HF G+  RG+P  +   + EG + PP  PP
Sbjct: 34446 HFRGQAARGRPHAQRLQHPEGVDAPPRPPP 34535


>scaffold_23
          Length = 674037

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 14/27 (51%), Positives = 14/27 (51%)
 Frame = -2

Query: 47    GRRRRGQPEPEVELNSEGEEVPPDDPP 73
             GRRRR Q  PE  L       PPDD P
Sbjct: 29279 GRRRRRQDPPEQRLRLPEGPAPPDDAP 29199


>scaffold_7
          Length = 2177197

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 15/34 (44%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
 Frame = -3

Query: 41     IRKHFLGRRRRGQ-PEPEVELNSEGEEVPPDDPP 73
              +R    GRR RG+ P PE    +EG   PP  PP
Sbjct: 307460 LRPRVRGRRTRGRRPRPEGAEGAEGATDPPQSPP 307359


>scaffold_110
          Length = 43059

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
 Frame = +3

Query: 248  GRGPDGYQVSELRTWLERYDEQL---HGARPRR 277
            GRG  G ++ ELR   ER D++L   HG R RR
Sbjct: 6972 GRGGHGRELLELRVAEERVDDELDVGHGERHRR 7070


>scaffold_9
          Length = 1602328

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 16/43 (37%), Positives = 24/43 (55%)
 Frame = +2

Query: 162     QRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAAR 204
               +RDV   ++   E H  D     D+P + P+ R+L+DRV  AR
Sbjct: 1521023 RRDVFLPVVAEREGH--DARQDADAPRVHPRVRALDDRVEFAR 1521145


>scaffold_2
          Length = 3465789

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 13/21 (61%), Positives = 15/21 (71%)
 Frame = +1

Query: 44      HFLGRRRRGQPEPEVELNSEG 64
               H  GRRRRG+PEPE E  + G
Sbjct: 1863478 HPHGRRRRGEPEPERERVAPG 1863540


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.322    0.139    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 30,817,738
Number of Sequences: 1185
Number of extensions: 724371
Number of successful extensions: 5156
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 52
Number of HSP's that attempted gapping in prelim test: 1221
Number of HSP's gapped (non-prelim): 4542
length of query: 278
length of database: 18,886,859
effective HSP length: 103
effective length of query: 175
effective length of database: 18,764,804
effective search space: 3283840700
effective search space used: 3283840700
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ENSMUST00000082426 # Protein # Iodothyronine deiodinase 1 (DI1)
# Mus musculus # Complete
         (257 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_8                                                             59   5e-09
scaffold_2                                                             39   0.008
scaffold_18                                                            30   2.9  
scaffold_28                                                            29   5.0  
scaffold_405                                                           29   6.6  
scaffold_10                                                            28   8.6  
scaffold_6                                                             28   8.6  

>scaffold_8
          Length = 1961717

 Score = 59.3 bits (142), Expect = 5e-09
 Identities = 50/166 (30%), Positives = 80/166 (48%), Gaps = 10/166 (6%)
 Frame = -1

Query: 89      GGLAPNCTVVCLSGQKCNIWDFIQGSRPLVLNFGSCTXPSFLLKFDQFKRLVDDFASTAD 148
               G  AP+  +  L G K ++      ++PLV+  GS + P F     +   L  +    AD
Sbjct: 1757603 GDAAPDVALWRLDGSKTSLLAGRDAAKPLVVVAGSVS*PPFRRVGPELDALARECPG-AD 1757427

Query: 149     FLIIYIEEAHATDGWAFKN------NVDIRQHRSLQERVRAARMLLA-RSPQCPVVVDTM 201
               F  +Y+ EAHA D W  ++       V + Q R+L +RV  AR   A      PVVVD  
Sbjct: 1757426 FTFVYVAEAHAADEWPLRSARFAAAPVVVDQPRTLADRVALARRYAADYGIASPVVVDDP 1757247

Query: 202     QNQS-SQLYAALPERLYVIQEGRICYKGKAGPWNY--NPEEVRAVL 244
                +++  + YA  P RLYV++   + +  +    +Y  +  E+RA+L
Sbjct: 1757246 TDEAFERAYAPWPLRLYVVRGAALAWIAEPDGASYERSVAELRAML 1757109


>scaffold_2
          Length = 3465789

 Score = 38.5 bits (88), Expect = 0.008
 Identities = 35/116 (30%), Positives = 55/116 (47%), Gaps = 3/116 (2%)
 Frame = -3

Query: 136     FKRLVDDFASTADFLIIYIEEAHATDGWAFKNNVDIRQHRSLQERVRAARMLLARSPQCP 195
               F+R  D+  ST+   + + ++A A+ G+AF         R  Q ++  +R+    SP+  
Sbjct: 1482469 FRRSKDE--STSRGALEFFQDASASAGFAFPQ-------RDGQRKLWQSRLAKQWSPREA 1482317

Query: 196     VVVDTMQNQSSQLYAALPERLYVIQEGRICYKGKAGP---WNYNPEEVRAVLEKLC 248
                 V + +   + L  A PERL+V  EGR  ++    P   W Y P   RA LE  C
Sbjct: 1482316 NDVSSSEIAMAALVDAEPERLHVCYEGRETFEVAIDPPKSWRYGPVVGRA-LEAFC 1482152



 Score = 28.9 bits (63), Expect = 6.6
 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
 Frame = -3

Query: 134     DQFKRLVDDFASTADFLIIYIEEAHATDG--WAFKNNVDIRQHRSLQERVRAARMLL 188
               D+F  +VDD A+ A  L + + EA A DG     +  VD+   R   E V+   +LL
Sbjct: 2509093 DEFAHVVDDDAALALHLHVAVAEAAAEDGNNDGQRRAVDLGHERRAHEAVQRRDVLL 2508923


>scaffold_18
          Length = 809113

 Score = 30.0 bits (66), Expect = 2.9
 Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 7/68 (10%)
 Frame = -2

Query: 174    HRSLQERVRAARMLLAR-----SPQCPVVVDTMQNQSSQLYAALPERLYVIQ--EGRICY 226
              H S+ +R  AA   L+      +    +V+D+M +     Y   P R+YV++   G I Y
Sbjct: 372183 HASIDDRKAAAEKALSLLRLKIAGPVTMVLDSMDDALEAAYETRPWRVYVVEVATGAIAY 372004

Query: 227    KGKAGPWN 234
                  GP N
Sbjct: 372003 ASGPGPMN 371980


>scaffold_28
          Length = 481284

 Score = 29.3 bits (64), Expect = 5.0
 Identities = 18/46 (39%), Positives = 25/46 (54%)
 Frame = -3

Query: 115    RPLVLNFGSCTXPSFLLKFDQFKRLVDDFASTADFLIIYIEEAHAT 160
              +PL L+ G+ T  SF L++    R  D  A   D+LI Y+E  H T
Sbjct: 252292 KPLYLDAGAPTANSFQLRWSPPLR--DGGAEIVDYLIHYVEVRHLT 252161


>scaffold_405
          Length = 7358

 Score = 28.9 bits (63), Expect = 6.6
 Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 2/57 (3%)
 Frame = +1

Query: 134  DQFKRLVDDFASTADFLIIYIEEAHATDG--WAFKNNVDIRQHRSLQERVRAARMLL 188
            D+F  +VDD A+ A  L + + EA A DG     +  VD+   R   E V+   +LL
Sbjct: 7081 DEFAHVVDDDAALALHLHVAVAEAAAEDGNNDGQRRAVDLGHERRAHEAVQRRDVLL 7251


>scaffold_10
          Length = 1654512

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 18/56 (32%), Positives = 30/56 (53%)
 Frame = -1

Query: 3      LPQLWLWLKRLVIFLQVALEVAVGKVLMTLFPGRVKQSILAMGQKTGMARNPRFAP 58
              LPQL L  +  ++ L +ALE+  G+VL     GR ++ + ++G +    R  R  P
Sbjct: 993840 LPQLRLLPRAPLLLLALALELLGGEVLHARGHGRPRRHVWSVGVRRCRRRRARPPP 993673


>scaffold_6
          Length = 2755913

 Score = 28.5 bits (62), Expect = 8.6
 Identities = 15/71 (21%), Positives = 28/71 (39%), Gaps = 13/71 (18%)
 Frame = -1

Query: 59      DNWVPTFFSIQYFWFVLKVR---WQRLEDRAEFG----------GLAPNCTVVCLSGQKC 105
               D W P    ++Y W+    R   WQ+  D   FG           +A     +  +   C
Sbjct: 1073204 DLWCPRSVDVEYMWYQWLPRSSIWQKRSDGIHFG*RPQRMEMRPVVAQRSVTMSKT*PNC 1073025

Query: 106     NIWDFIQGSRP 116
               ++W  ++ ++P
Sbjct: 1073024 DVWSNLRAAQP 1072992


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.326    0.140    0.454 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,727,316
Number of Sequences: 1185
Number of extensions: 294132
Number of successful extensions: 1853
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 80
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 1080
Number of HSP's gapped (non-prelim): 1300
length of query: 257
length of database: 18,886,859
effective HSP length: 102
effective length of query: 155
effective length of database: 18,765,989
effective search space: 2908728295
effective search space used: 2908728295
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 62 (28.5 bits)
TBLASTN 2.2.13 [Nov-27-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= ENSMUST00000082432 # Protein # Iodothyronine deiodinase 2 (DI2)
# Mus musculus # Complete
         (266 letters)

Database: genome.fa 
           1185 sequences; 56,660,578 total letters

Searching...................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

scaffold_8                                                             55   9e-08
scaffold_10                                                            30   3.1  
scaffold_31                                                            30   4.1  
scaffold_53                                                            29   5.3  

>scaffold_8
          Length = 1961717

 Score = 55.1 bits (131), Expect = 9e-08
 Identities = 48/155 (30%), Positives = 71/155 (45%), Gaps = 4/155 (2%)
 Frame = -1

Query: 105     DGAECHLLDFASAERPLVVNFGSATXPPFTRQLPAFRQLVEEFSSVADFLLVYIDEAHPS 164
               DG++  LL    A +PLVV  GS + PPF R  P    L  E    ADF  VY+ EAH +
Sbjct: 1757567 DGSKTSLLAGRDAAKPLVVVAGSVS*PPFRRVGPELDALARECPG-ADFTFVYVAEAHAA 1757391

Query: 165     DGWAVPGD--SSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMDNNANVAYGV 222
               D W +     ++    V + R   DR A A +    + +     VV D  D     AY  
Sbjct: 1757390 DEWPLRSARFAAAPVVVDQPRTLADRVALARRYAADYGIASPV-VVDDPTDEAFERAYAP 1757214

Query: 223     AFERVCIVQRRKIAYLGGKGPFSY--NLQEVRSWL 255
                  R+ +V+   +A++      SY  ++ E+R+ L
Sbjct: 1757213 WPLRLYVVRGAALAWIAEPDGASYERSVAELRAML 1757109



 Score = 29.6 bits (65), Expect = 4.1
 Identities = 16/38 (42%), Positives = 21/38 (55%)
 Frame = +3

Query: 71     AYKQVKLGEDAPNSSVVHVSNPESGNNYASEKTADGAE 108
              A K  K GE+ PN+ V +   P+     AS KT +GAE
Sbjct: 237351 ADKNTKDGEEGPNTRVEYTDKPDGDG--ASAKTPEGAE 237458


>scaffold_10
          Length = 1654512

 Score = 30.0 bits (66), Expect = 3.1
 Identities = 27/97 (27%), Positives = 38/97 (39%)
 Frame = -2

Query: 78      GEDAPNSSVVHVSNPESGNNYASEKTADGAECHLLDFASAERPLVVNFGSATXPPFTRQL 137
               G        V V  P + +  A+   A      LL   +A RP   + G    PP     
Sbjct: 1103585 GSSTDGEGAVKVKKPRNSSKAAATMAALWV-ARLLPLVAASRP---SAGGVAAPPAEADS 1103418

Query: 138     PAFRQLVEEFSSVADFLLVYIDEAHPSDGWAVPGDSS 174
               PA+R+LVE +S     L    D    ++ W+ P  SS
Sbjct: 1103417 PAWRRLVEAWSRRR--LDAGDDACEHAECWSCPTASS 1103313


>scaffold_31
          Length = 470201

 Score = 29.6 bits (65), Expect = 4.1
 Identities = 31/94 (32%), Positives = 47/94 (50%), Gaps = 7/94 (7%)
 Frame = +1

Query: 158    IDEAHPSDGWAVPGDSSLSFEVKKHRNQEDRCAAAHQLLERFSLPPQCQVVADRMD--NN 215
              ++E H  DG A PGD       ++ R +  R   A ++      PP+ +V A R +  + 
Sbjct: 426940 VEERH-GDGLA-PGDVRRQHRARERRERR-RLVVAEEVH-----PPRPRVRAPRQELPDR 427095

Query: 216    ANVAYG--VAFERVCIVQR---RKIAYLGGKGPF 244
                VA G  +  E+V +V R    +IAYLGG+G F
Sbjct: 427096 GRVALGGPLEQEQVEVVLRGAHERIAYLGGRGGF 427197


>scaffold_53
          Length = 203801

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 27/92 (29%), Positives = 40/92 (43%), Gaps = 14/92 (15%)
 Frame = -3

Query: 126   GSATXPPFTRQLPAFRQLVEEFSSVADF-LLVYIDEAHPSDG-------WAVPGDSSLSF 177
             G+    P TR LP +  L+   S  A +  L++I  +HP+         WA    S  +F
Sbjct: 11112 GARRRAPSTRGLPTYDSLLLALSWRAIYRALIHIPSSHPAGDMLNAASTWAST*VSISNF 10933

Query: 178   ------EVKKHRNQEDRCAAAHQLLERFSLPP 203
                   + ++ R + D   AAH LLE    PP
Sbjct: 10932 AHQQGPQHRRLRLKRDLNTAAHALLETSEAPP 10837


  Database: genome.fa
    Posted date:  Feb 16, 2009  1:41 PM
  Number of letters in database: 56,660,578
  Number of sequences in database:  1185
  
Lambda     K      H
   0.321    0.135    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,437,132
Number of Sequences: 1185
Number of extensions: 284270
Number of successful extensions: 1401
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 75
Number of HSP's successfully gapped in prelim test: 34
Number of HSP's that attempted gapping in prelim test: 764
Number of HSP's gapped (non-prelim): 1010
length of query: 266
length of database: 18,886,859
effective HSP length: 102
effective length of query: 164
effective length of database: 18,765,989
effective search space: 3077622196
effective search space used: 3077622196
frameshift window, decay const: 40,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
