TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000064_1.0 # Protein # Selenoprotein 15 (Sel15) # Tetraodon nigroviridis # Complete (153 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** Database: genome.fa Posted date: Feb 25, 2008 11:55 AM Number of letters in database: 20,171,213 Number of sequences in database: 2960 Lambda K H 0.320 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2960 Number of Hits to DB: 2,262,063 Number of extensions: 22122 Number of successful extensions: 70 Number of sequences better than 10.0: 1 Number of HSP's gapped: 70 Number of HSP's successfully gapped: 1 Length of query: 153 Length of database: 6,723,737 Length adjustment: 88 Effective length of query: 65 Effective length of database: 6,463,257 Effective search space: 420111705 Effective search space used: 420111705 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 41 (20.4 bits)