TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000039_1.0 # Protein # Methionine sulfoxide reductase A (MsrA) # Drosophila melanogaster # Complete (246 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY02707|2002-09-10|ds-DNA... 27 7.7 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY02707|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 3255 Score = 26.9 bits (58), Expect = 7.7, Method: Composition-based stats. Identities = 12/43 (27%), Positives = 25/43 (58%) Frame = +1 Query: 130 HDEEQKQVAHASKLEEQERRAPEIITTEIASKENFYPAEAYHQ 172 HD+ + K+ +Q+++ IIT +IA+K +Y + +H+ Sbjct: 727 HDQINNGSTFSPKISDQKKKTQNIITLQIATK-FYYSLKNFHK 852 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000047_1.0 # Protein # Selenophosphate synthetase 1 (SPS1) # Drosophila melanogaster # Complete (398 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds-DNA... 62 3e-10 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 6010 Score = 62.4 bits (150), Expect = 3e-10, Method: Composition-based stats. Identities = 72/312 (23%), Positives = 122/312 (39%), Gaps = 52/312 (16%) Frame = -2 Query: 48 GRGCKVPQDVLSKLVSALQQDYSAQDQEPQFLNVAIPRIGIGL----DCSVI-----PLR 98 G G KVP +VLS + +L S P + +G+ DC + Sbjct: 3669 GCGSKVPSNVLSNSLKSLNIYTS-------------PNVYLGVEGCDDCCIFVHSKSKRS 3529 Query: 99 HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLLA---VSTKMT 155 LVQT DFF +DD Y++G++ + LSD+Y+MG T + +L+ + K+ Sbjct: 3528 EESPALVQTIDFFKSFIDDEYILGEVIAIHCLSDIYSMGGTGICALCVLIIKDNIEKKLQ 3349 Query: 156 EKERDVVIPLIMR----------GFKDSALE--AGTTVTG-------------------- 183 ++ +++ + G + E G VTG Sbjct: 3348 QRLENILTGCCQKLKEEKCVLSGGHTCAGTENYVGLAVTGKIKKKKNNNKDKEEIRKSQN 3169 Query: 184 GQSVVNPWCTIGGVASTICQPNE-----YIVPDNAV---VGDVLVLTKPLGTQVAVNAHQ 235 +SV++ +G ++I + NE + +P ++ GDV++ TK G + AH Sbjct: 3168 NESVLDKTNQVGN--NSINKVNELNEAYHFLPKGSIEVKPGDVIITTKVFGFGFIMAAHI 2995 Query: 236 WIDQPERWNRIKLVVSEEDVRKAYHRAMNSMARLNRVAARLMHKYNAHGATDITGFGLLG 295 RW + ++ M NR K NA TD+TGFG+LG Sbjct: 2994 SKKAKARW---------------IYNCLDEMLISNRKGGLYFLKNNAKACTDVTGFGILG 2860 Query: 296 HAQTLAAHQKKD 307 H + +++ Sbjct: 2859 HLNEMIKCSRRE 2824 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000048_1.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Drosophila melanogaster # Complete (370 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds-DNA... 43 2e-04 Plasmodium_yoelii_yoelii_str._17XNL|MALPY00282|2002-09-10|ds-DNA... 28 5.7 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 6010 Score = 43.1 bits (100), Expect = 2e-04, Method: Composition-based stats. Identities = 29/96 (30%), Positives = 44/96 (45%), Gaps = 1/96 (1%) Frame = -2 Query: 184 QPGDVLVLTKPLGGQMAMDAHLWQLNQTEKYK-KLLSECSDADIKETFEIAVKSMTYLNK 242 +PGDV++ TK G M AH+ ++K K + + C D M N+ Sbjct: 3060 KPGDVIITTKVFGFGFIMAAHI-----SKKAKARWIYNCLD------------EMLISNR 2932 Query: 243 NAALLMHKYQAHCATDITGFGLLGHANNLAQFQKEK 278 L K A TD+TGFG+LGH N + + + + Sbjct: 2931 KGGLYFLKNNAKACTDVTGFGILGHLNEMIKCSRRE 2824 Score = 43.1 bits (100), Expect = 2e-04, Method: Composition-based stats. Identities = 26/88 (29%), Positives = 41/88 (46%), Gaps = 4/88 (4%) Frame = -2 Query: 23 GUSCKIPQKVLEKYLRGTEIENKNNDGYLIGSGMDCAVIPLKRHK----DYLLIQTVDFF 78 G K+P VL L+ I N + DC + + K L+QT+DFF Sbjct: 3669 GCGSKVPSNVLSNSLKSLNIYTSPNVYLGVEGCDDCCIFVHSKSKRSEESPALVQTIDFF 3490 Query: 79 YPMVNDPELLGRIALANVLSDVYAVGVT 106 ++D +LG + + LSD+Y++G T Sbjct: 3489 KSFIDDEYILGEVIAIHCLSDIYSMGGT 3406 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00282|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 13925 Score = 28.5 bits (62), Expect = 5.7, Method: Composition-based stats. Identities = 18/49 (36%), Positives = 23/49 (46%) Frame = -3 Query: 219 SECSDADIKETFEIAVKSMTYLNKNAALLMHKYQAHCATDITGFGLLGH 267 S CS+ KET +IA + Y N A + + H DI G L GH Sbjct: 7536 SICSEGWTKETEKIACLELGYHNVKA----NGFSQHLCNDIAGENLCGH 7402 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000040_1.0 # Protein # Selenoprotein 15 (Sel15) # Drosophila melanogaster # Complete (178 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY00867|2002-09-10|ds-DNA... 27 5.3 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00867|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 19695 Score = 26.9 bits (58), Expect = 5.3, Method: Composition-based stats. Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = -3 Query: 127 QETLSITKWNTDTVEEFFETHLAKDGAGKNSYS 159 QETL K++ + E + H+ DG+G YS Sbjct: 18334 QETLDEIKYSEEVCTEQIDIHILLDGSGSIGYS 18236 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000041_1.0 # Protein # Selenoprotein H1 (SelH1) # Drosophila melanogaster # Complete (249 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000042_1.0 # Protein # Selenoprotein H2 (SelH2) # Drosophila melanogaster # Complete (146 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000043_1.0 # Protein # Selenoprotein H3 (SelH3) # Drosophila melanogaster # Complete (143 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY00925|2002-09-10|ds-DNA... 27 2.3 Plasmodium_yoelii_yoelii_str._17XNL|MALPY00008|2002-09-10|ds-DNA... 27 3.7 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00925|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 10981 Score = 27.3 bits (59), Expect = 2.3, Method: Composition-based stats. Identities = 15/35 (42%), Positives = 21/35 (60%), Gaps = 1/35 (2%) Frame = -3 Query: 26 SMIYIEHTYECPIF-QTKADECGTFFTQRIPERKF 59 S+IYI HT+E IF TK + C ++ T I E+ Sbjct: 746 SLIYI*HTFENVIFLSTKLNYCCSWKTSFITEKSL 642 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00008|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 25321 Score = 26.6 bits (57), Expect = 3.7, Method: Composition-based stats. Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = -3 Query: 16 SSDEHFSKDRSMIYIEHTYECPIF 39 +S EHF + R M+ + Y CP+F Sbjct: 12824 NSQEHFWELRKMVKNYNDYNCPLF 12753 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000044_1.0 # Protein # Selenoprotein K1 (SelK1) # Drosophila melanogaster # Complete (110 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000045_1.0 # Protein # Selenoprotein K2 (SelK2) # Drosophila melanogaster # Complete (118 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000046_1.0 # Protein # Selenoprotein T (SelT) # Drosophila melanogaster # Complete (198 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY02908|2002-09-10|ds-DNA... 27 5.1 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY02908|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 2387 Score = 27.3 bits (59), Expect = 5.1, Method: Composition-based stats. Identities = 11/25 (44%), Positives = 15/25 (60%) Frame = -3 Query: 3 RLTGRNVALLVLCLCAGYALVFAEG 27 R+ GR + L+LCLC A+ AE Sbjct: 924 RIAGRRMKRLILCLCGMLAISAAEA 850 Database: genome.fa Posted date: Feb 25, 2008 11:55 AM Number of letters in database: 20,171,213 Number of sequences in database: 2960 Lambda K H 0.314 0.131 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2960 Number of Hits to DB: 36,981,699 Number of extensions: 479695 Number of successful extensions: 1701 Number of sequences better than 10.0: 14 Number of HSP's gapped: 1698 Number of HSP's successfully gapped: 17 Length of database: 6,723,737 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 15 ( 6.8 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.1 bits) S2: 42 (20.8 bits)