CLUSTAL W (1.81) Multiple Sequence Alignments Sequence type explicitly set to Protein Sequence format is Pearson Sequence 1: subseq_8000_4000_.[1811_3967]. 719 aa Sequence 2: EFsec_2 935 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 42.6982 (Partial alignment) Sequences (2:2) Aligned. Score: 90.8021 Guide tree file created: [clustalw.dnd] Start of Multiple Alignment There are 1 groups Aligning... Group 1: Sequences: 2 Score:5465 Alignment Score 2101 CLUSTAL-Alignment file created [clustalw.aln] clustalw.aln CLUSTAL W (1.81) multiple sequence alignment subseq_8000_4000_.[1811_3967]. MINVNIGVLGHVDSGKTSLCRCLSEVLSTCALDKHRQSQEKGITIDLGFS EFsec_2 MININVGVLGHVDSGKTSLCKCLSQVLSTCALDKHKESKERGITIDLGFS ***:*:**************:***:**********::*:*:********* subseq_8000_4000_.[1811_3967]. SFYIKKKK-------------SQTSTSQTTTSQNQHGEIQNNKIVPKHGP EFsec_2 SFYIKKKKKKKYDVNQKREIQTNLLNDQTTDQKESFLYCKREEKNDLHNI ******** :: ..*** .::.. :.:: *. subseq_8000_4000_.[1811_3967]. NKNECIPDQNDVKKEDINNDCEIENIQNISDENELIQICLVDCPGHHSLI EFsec_2 QYDKTNIEYNEEQNCYDYTNDKHHSNNIYFYDEEIIQICLVDCPGHHSLL : :: : *: :: .: : .. : ::*:**************: subseq_8000_4000_.[1811_3967]. KCIVMGAKIMDMIFLVIDINKGIQKQTIECLVLCQFVKSDIIIILNKIDL EFsec_2 KSIIMGSEITDIIILVIDINKGIQKQTIECLVLCKIINCDIIIVLNKIDL *.*:**::* *:*:********************::::.****:****** subseq_8000_4000_.[1811_3967]. IPLHEREKKINTMKKKIKDAINRTS-LKKLSYYIVSLSANKKNQ------ EFsec_2 IPLHLREKKINLMKKKIQEAFYKFKSLQSLNYHIVSISANVKGKDNKDVE **** ****** *****::*: : . *:.*.*:***:*** *.: subseq_8000_4000_.[1811_3967]. -------------------------------------------------- EFsec_2 GDPPNLLSHDKKGVKILLSGDNKDLPILLSDDKKDPPILLNDDKKDPPNL subseq_8000_4000_.[1811_3967]. -----------------------NDNEKIDHVEIEENAISMCRNNPNEIN EFsec_2 LNDDKKDPPNLLNDDKKDPPNLLNDDKKDPSILLSDDKKDPPNLLNDDKK **::* : :.:: . . :: : subseq_8000_4000_.[1811_3967]. PRFNILKKEK--------------------------HTSENINELIELLK EFsec_2 DPPNLLNDDKKDPSILLNDHMNEWSQNKSSSDIPFMNKTINIKEIINVLT *:*:.:* :.: **:*:*::*. subseq_8000_4000_.[1811_3967]. SVIKIPVRETNPSFEDFYFLYDHSFEIKGKGMVYTGTVIKGKITINSNIS EFsec_2 NIIKIPEREIS-TYEDFYFLYDHSFNIKGKGTIFTGTVIKGKIKINCNVT .:**** ** . ::***********:***** ::*********.**.*:: subseq_8000_4000_.[1811_3967]. ILPLNIKGKIKDIQSFKKKQKEGTSGNRLSFLISHDNIKNLKKVERGIIV EFsec_2 ILPLNETGKIKEIQSFKKNVHEGKKGSRLSLLILNNNIKNIKKNERGVII ***** .****:******: :**..*.***:** ::****:** ***:*: subseq_8000_4000_.[1811_3967]. HEKSNICNFTVFICKIKLIEFYKKSIKNKELLTCIIGFASSHSYGFFFKK EFsec_2 YENSNISYFSMFICKVKQVEFYKKDLNNLEHFICIIGFSSTECYGYFFKK :*:***. *::****:* :*****.::* * : *****:*:..**:**** subseq_8000_4000_.[1811_3967]. LK------------------------------------------------ EFsec_2 IKNKYETNESKKINYKENSQGHDVFPTLLEDTLNVDKRFDRKGNYLLINK :* subseq_8000_4000_.[1811_3967]. ---TDPEKEQTINGHNNGHNNGHNNGHSNGHSNGHNN------------- EFsec_2 LCYDDTQKENNNNNKKNKKNNNNKKNKKNGNKNEHSDDIIKNEHGEDINK *.:**:. *.::* :**.:::.:.**:.* *.: subseq_8000_4000_.[1811_3967]. ---------------GHSNGHSNGHNNEQNNGQNNGERDSTCLSNNIGFD EFsec_2 NEHSDDNNKNEHGDDNNKNEHGDYINNNHLNDYINNNHLNDDVNNNHLND .:.* *.: **:: *. *.:: . :.** * subseq_8000_4000_.[1811_3967]. RNGNYLIIDEVEYLNHINDETVS---------------ENDLFEDEKDEE EFsec_2 DVNNSHLNDDVNN-NHLNDDVNNNHLNDDVNNNHLNDDVNNSHLNNYDDD .* : *:*: **:**:. . *: . :: *:: subseq_8000_4000_.[1811_3967]. IYFLVILKKKMYGFNGEKCIFLKNDENINCRICLHGNIVDIIKDSAINSQ EFsec_2 IYFLVVMKRKIHCFENEKCIFLKNDEHTNCRICLYGEIIEIIDNNCTYKK *****::*:*:: *:.**********: ******:*:*::**.:.. .: subseq_8000_4000_.[1811_3967]. INNSTFVMNKKPCLNEHEDYKNLKIMKEKEKIGLIEKVIDNTTLLCKNVF EFsec_2 DD--SFVFNKQPNIYKYEEYKDLKILKEKNKKGNIDKIIDEHTIICKKLF : :**:**:* : ::*:**:***:***:* * *:*:**: *::**::* subseq_8000_4000_.[1811_3967]. TSSNQVTAYLTQKIYLIPLEHQKNK------NVCKTATIGYIAKSFAKNG EFsec_2 QSSNQVIPYIYKKIYIVKSTYDQKNGNTTSGNTSNVLHTGTIIKPFAKQG ***** .*: :***:: ::::: *..:. * * *.***:* subseq_8000_4000_.[1811_3967]. KFIVHFDDDISHIKDNIKSYIIMIKYYKDIFTKKKIFM EFsec_2 KAIVSFDEDISYLKNDIKSYNFLLVYYKDVFSKKKVFL * ** **:***::*::**** ::: ****:*:***:*: clustalw.dnd (subseq_8000_4000_.[1811_3967].:0.28651,EFsec_2:0.28651);