TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000049_1.0 # Protein # Methionine sulfoxide reductase A1 (MsrA1) # Anopheles gambiae # Complete (236 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY00946|2002-09-10|ds-DNA... 29 2.2 Plasmodium_yoelii_yoelii_str._17XNL|MALPY01972|2002-09-10|ds-DNA... 28 2.4 Plasmodium_yoelii_yoelii_str._17XNL|MALPY02407|2002-09-10|ds-DNA... 28 2.6 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00946|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 10425 Score = 28.9 bits (63), Expect = 2.2, Method: Composition-based stats. Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = +2 Query: 108 RMKRQYMSLILYHNEQQRQIAEASRAEEQVK 138 R ++QYMSL L+ N+ + + ++S E ++K Sbjct: 8456 RTQKQYMSLNLFSNKNKNEEYDSSSNESEIK 8548 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01972|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 4410 Score = 28.5 bits (62), Expect = 2.4, Method: Composition-based stats. Identities = 12/53 (22%), Positives = 28/53 (52%) Frame = -1 Query: 95 DLFWNNHEYGLTTRMKRQYMSLILYHNEQQRQIAEASRAEEQVKRAPEQIITE 147 D+FWNN++ G +++ Y + N+ +IA+ Q + P +++++ Sbjct: 3405 DIFWNNNKVGKSSQAVYAYFVCVCLENKPSSKIAK*QNC--QAAKLPSKVLSK 3253 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY02407|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 3950 Score = 28.5 bits (62), Expect = 2.6, Method: Composition-based stats. Identities = 32/161 (19%), Positives = 70/161 (43%), Gaps = 11/161 (6%) Frame = +2 Query: 65 ESPAYKKMGDHTEVIEIDYDPQTISY--NDLLDLFWNNHEYGLTTRMKRQYMSLILYHNE 122 E+P Y+K+ ++++ D + + I+Y N + + N + ++++ + Sbjct: 2189 ENPEYEKLSSKNKIVQNDINHEMINYKNNAIFNKSLQNEKASQCLQLEKTFDDKT---KN 2359 Query: 123 QQRQIAEASRAEEQVKRAP---EQIITEIAPAGPFYPAEN---YHQKYRLQGHTDLAKGI 176 + +I EA + +Q+ AP +Q+ +G A+ Y +K +L +L I Sbjct: 2360 ESGKIGEAPKESDQLCEAPKESDQLCEAPKESGQLCEAQKGYGYIEKDKLYIINNLKNKI 2539 Query: 177 GLTPDLLHTSHVAARL---NGYLIGVSGLKQFEDEADLLGL 214 +T ++ + NG + GLK E+ + L+ L Sbjct: 2540 KVTCSKINNISFVEEMHYSNGSYKIILGLKNVENNSYLIKL 2662 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000050_1.0 # Protein # Methionine sulfoxide reductase A2 (MsrA2) # Anopheles gambiae # Complete (236 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY00946|2002-09-10|ds-DNA... 29 2.0 Plasmodium_yoelii_yoelii_str._17XNL|MALPY01972|2002-09-10|ds-DNA... 28 2.4 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00946|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 10425 Score = 28.9 bits (63), Expect = 2.0, Method: Composition-based stats. Identities = 11/31 (35%), Positives = 21/31 (67%) Frame = +2 Query: 108 RMKRQYMSLILYHNEQQRQIAEASRAEEQVK 138 R ++QYMSL L+ N+ + + ++S E ++K Sbjct: 8456 RTQKQYMSLNLFSNKNKNEEYDSSSNESEIK 8548 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01972|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 4410 Score = 28.5 bits (62), Expect = 2.4, Method: Composition-based stats. Identities = 12/53 (22%), Positives = 28/53 (52%) Frame = -1 Query: 95 DLFWNNHEYGLTTRMKRQYMSLILYHNEQQRQIAEASRAEEQVKRAPEQIITE 147 D+FWNN++ G +++ Y + N+ +IA+ Q + P +++++ Sbjct: 3405 DIFWNNNKVGKSSQAVYAYFVCVCLENKPSSKIAK*QNC--QAAKLPSKVLSK 3253 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000055_1.0 # Protein # Selenophosphate synthetase 1 (SPS1) # Anopheles gambiae # Complete (396 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds-DNA... 69 4e-12 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 6010 Score = 68.9 bits (167), Expect = 4e-12, Method: Composition-based stats. Identities = 74/307 (24%), Positives = 112/307 (36%), Gaps = 52/307 (16%) Frame = -2 Query: 51 KVPKEVLEKLVSSLQQDYMQDPDQPFMPMSSPRIGIGL----DCSVI-----PLRHGGLC 101 KVP VL + SL +SP + +G+ DC + Sbjct: 3657 KVPSNVLSNSLKSLN------------IYTSPNVYLGVEGCDDCCIFVHSKSKRSEESPA 3514 Query: 102 MVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTEVDNMLMLLAVSTKMIEKERDVVI 161 +VQT DFF +DD Y++G++ + LSD+Y+MG T + + +L+ IEK+ + Sbjct: 3513 LVQTIDFFKSFIDDEYILGEVIAIHCLSDIYSMGGTGICALCVLIIKDN--IEKKLQQRL 3340 Query: 162 PLIMRGFKDSALEAGTSVTGGHSVVNPWCTIGGVATTI----------------CQQNEF 205 I+ G E ++GGH+ +G T Q NE Sbjct: 3339 ENILTGCCQKLKEEKCVLSGGHTCAGTENYVGLAVTGKIKKKKNNNKDKEEIRKSQNNES 3160 Query: 206 I------VPDNAV---------------------VGDVLVLTKALGTQVAVNAHQWLDQS 238 + V +N++ GDV++ TK G + AH Sbjct: 3159 VLDKTNQVGNNSINKVNELNEAYHFLPKGSIEVKPGDVIITTKVFGFGFIMAAHISKKAK 2980 Query: 239 ERWNRIKLVVSEEDVRKAYHRAMDSMSRLNRVAARLMHKYNAHGATDVTGFGLLGHAQTL 298 RW + +D M NR K NA TDVTGFG+LGH + Sbjct: 2979 ARW---------------IYNCLDEMLISNRKGGLYFLKNNAKACTDVTGFGILGHLNEM 2845 Query: 299 ASHQKNE 305 + E Sbjct: 2844 IKCSRRE 2824 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000056_1.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Anopheles gambiae # Complete (369 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds-DNA... 46 2e-05 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01772|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 6010 Score = 46.2 bits (108), Expect = 2e-05, Method: Composition-based stats. Identities = 39/123 (31%), Positives = 60/123 (48%), Gaps = 10/123 (8%) Frame = -2 Query: 19 STLRGUGSKVPQDVLNRLLAG--VYGEQLGDKDGKGPNKEEGVGIGLDSSVIAL------ 70 +T G GSKVP +VL+ L +Y PN GV G D I + Sbjct: 3681 NTCGGCGSKVPSNVLSNSLKSLNIY---------TSPNVYLGVE-GCDDCCIFVHSKSKR 3532 Query: 71 -KHDLFLVQSVDFFYPLIDDPFMLGKIALANVVSDVFAVGATEIDQI-KLIVTAPTEFTE 128 + LVQ++DFF IDD ++LG++ + +SD++++G T I + LI+ E Sbjct: 3531 SEESPALVQTIDFFKSFIDDEYILGEVIAIHCLSDIYSMGGTGICALCVLIIKDNIEKKL 3352 Query: 129 QER 131 Q+R Sbjct: 3351 QQR 3343 Score = 35.4 bits (80), Expect = 0.043, Method: Composition-based stats. Identities = 26/89 (29%), Positives = 36/89 (40%) Frame = -2 Query: 186 QPGDALVLTKPLGTQLATNAYIWMGEQQSDSWAQLRERFTVADIEQTYRIALESMSRLNK 245 +PGD ++ TK G A+I ++ W Y L+ M N+ Sbjct: 3060 KPGDVIITTKVFGFGFIMAAHI--SKKAKARW--------------IYN-CLDEMLISNR 2932 Query: 246 TGAELMKKYGAHAATDVTGFGLYGHAENL 274 G K A A TDVTGFG+ GH + Sbjct: 2931 KGGLYFLKNNAKACTDVTGFGILGHLNEM 2845 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000051_1.0 # Protein # Selenoprotein 15 (Sel15) # Anopheles gambiae # Complete (163 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY00941|2002-09-10|ds-DNA... 27 5.0 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00941|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 6880 Score = 26.6 bits (57), Expect = 5.0, Method: Composition-based stats. Identities = 21/63 (33%), Positives = 32/63 (50%) Frame = -3 Query: 95 IKSDRPAKFPNLTIKYVRGLDPIVKLMDEQGNVKETLSINKWNTDTVQEFFETRLAKVED 154 IKS + K P + I D I+ MD+ NV LS N+ N++ + + +LAK Sbjct: 5558 IKSQKIYKIPRICIHR----DKILNKMDKT-NVDTKLSENRENSEKNNKKKKXQLAKNGK 5394 Query: 155 DDY 157 D+Y Sbjct: 5393 DNY 5385 TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000052_1.0 # Protein # Selenoprotein H1 (SelH1) # Anopheles gambiae # Complete (131 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000053_1.0 # Protein # Selenoprotein K1 (SelK1) # Anopheles gambiae # Complete (96 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= SPP00000054_1.0 # Protein # Selenoprotein T (SelT) # Anopheles gambiae # Complete (202 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done ***** No hits found ****** Database: genome.fa Posted date: Feb 25, 2008 11:55 AM Number of letters in database: 20,171,213 Number of sequences in database: 2960 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2960 Number of Hits to DB: 32,671,797 Number of extensions: 408221 Number of successful extensions: 1479 Number of sequences better than 10.0: 14 Number of HSP's gapped: 1478 Number of HSP's successfully gapped: 22 Length of database: 6,723,737 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 15 ( 6.9 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (20.8 bits) S2: 42 (20.8 bits)