TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= gi|45550694|ref|NP_649556.2| CG1427 CG1427-PA, isoform A [Drosophila melanogaster] (448 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY01707|2002-09-10|ds-DNA... 232 2e-66 Plasmodium_yoelii_yoelii_str._17XNL|MALPY02378|2002-09-10|ds-DNA... 28 7.5 Plasmodium_yoelii_yoelii_str._17XNL|MALPY01587|2002-09-10|ds-DNA... 28 7.7 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01707|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 11279 Score = 232 bits (592), Expect(2) = 2e-66, Method: Composition-based stats. Identities = 120/317 (37%), Positives = 189/317 (59%), Gaps = 5/317 (1%) Frame = -2 Query: 95 IGRSGDLLEAQPKAAGSTLLARLTNALILDLIRGIGLPSCAGCFLVPMCTGMTLTLCLQS 154 I RSG+L + QPK+AG++LL ++T ++ DLI+ G+ C +++P TGM ++ CL Sbjct: 8377 IFRSGNLEDVQPKSAGNSLLCKMTTNMLKDLIKTFGIKKCEDVYILPYATGMCISTCLLY 8198 Query: 155 LRKRRPGARYVLWSRIDQKSCFKAITATGLVPVVIPCLIKGESLNTNVDLFREKIKSLGV 214 L++ R ++YV+ SRID K+C+K + L +V+ + + LNT+ +K L Sbjct: 8197 LKRMRQNSKYVIISRIDHKTCYKCLDFCDLKYIVVDMIFNNDELNTDEKKIENLMKKLK- 8021 Query: 215 DSILCLYTTTSCFAPRNSDDIAEVSKLSKQWQIPHLVNNAYGLQAKEIVNQLE-CANRVG 273 + I C+ T TS +APRNSD+I ++S+L ++ IPHL+NN +GLQ I +++ C + G Sbjct: 8020 EKICCIITVTSSYAPRNSDNILKISQLCNKYDIPHLINNGFGLQCTYICKEIQKCYDSNG 7841 Query: 274 RIDYFVQSSDKNLLVPVGSAIVASFNESVLHDVASTYAGRASGSQSLDVLMTLLSLGRNG 333 RID+ VQS DKN L+P+ I+ S N+ ++ ++ TY GR + LD+ +TLL LG+N Sbjct: 7840 RIDFVVQSCDKNFLLPINGGIIFSSNQKIMTELKKTYPGRTPVNVYLDLFITLLELGKNK 7661 Query: 334 FRLLFDQRGENFNYLRENLRKFAEPRGEIVIDSRFNSISLAITLATL----AGDQMKSIT 389 L ++R +NFN++ E ++ +I + N IS+AI L L K I Sbjct: 7660 IMTLREERVKNFNWINEQVQNICSKYNLKLIKTTKNKISIAINLNNLYNYCGNTNPKIIN 7481 Query: 390 KLGSMLHMRGVSGARVI 406 LGS L R V+G RVI Sbjct: 7480 MLGSFLFYRNVTGHRVI 7430 Score = 38.9 bits (89), Expect(2) = 2e-66, Method: Composition-based stats. Identities = 16/37 (43%), Positives = 24/37 (64%) Frame = -3 Query: 61 LDSNNYPHKVGLGEREARIACKLVARRHYNFGHGIGR 97 L+ N + +GERE+RI +V +++ FGHGIGR Sbjct: 8571 LNRCNSEKNIKIGERESRIYSSIVRNKYFGFGHGIGR 8461 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY02378|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 3300 Score = 28.5 bits (62), Expect = 7.5, Method: Composition-based stats. Identities = 13/35 (37%), Positives = 22/35 (62%) Frame = -2 Query: 15 DNYLQLGLAAQRSKQRSFKELLEKRKLPKNGWSDE 49 +NY QLG+ ++S +KE+ EK K+ N + D+ Sbjct: 1499 NNYGQLGINYKKSIYTEYKEIGEKNKVGNNWYIDK 1395 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01587|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 14660 Score = 28.1 bits (61), Expect = 7.7, Method: Composition-based stats. Identities = 18/66 (27%), Positives = 32/66 (48%) Frame = -2 Query: 246 QIPHLVNNAYGLQAKEIVNQLECANRVGRIDYFVQSSDKNLLVPVGSAIVASFNESVLHD 305 QI ++ N+ + + +E N + + + S K + P+ I++SFN S L D Sbjct: 2980 QIKNIENDVNQYKKDYEIGIVEKINEIAKANKDKIESTKESIEPIIQNIMSSFNSSDLED 2801 Query: 306 VASTYA 311 +AS A Sbjct: 2800 IASNEA 2783 Database: genome.fa Posted date: Mar 4, 2008 12:20 PM Number of letters in database: 20,171,213 Number of sequences in database: 2960 Lambda K H 0.320 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2960 Number of Hits to DB: 8,697,383 Number of extensions: 112116 Number of successful extensions: 340 Number of sequences better than 10.0: 5 Number of HSP's gapped: 337 Number of HSP's successfully gapped: 6 Length of query: 448 Length of database: 6,723,737 Length adjustment: 100 Effective length of query: 348 Effective length of database: 6,427,737 Effective search space: 2236852476 Effective search space used: 2236852476 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 45 (21.9 bits)