TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= MAL8P1.86_changed | Plasmodium falciparum 3D7 | Sel3 protein|conserved Plasmodium selenoprotein, unknown function | protein | length=351 (351 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY01723|2002-09-10|ds-DNA... 202 2e-52 Plasmodium_yoelii_yoelii_str._17XNL|MALPY02589|2002-09-10|ds-DNA... 30 1.8 Plasmodium_yoelii_yoelii_str._17XNL|MALPY01965|2002-09-10|ds-DNA... 29 3.0 Plasmodium_yoelii_yoelii_str._17XNL|MALPY00824|2002-09-10|ds-DNA... 28 9.0 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01723|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 4336 Score = 202 bits (513), Expect = 2e-52, Method: Composition-based stats. Identities = 148/352 (42%), Positives = 194/352 (55%), Gaps = 2/352 (0%) Frame = +2 Query: 1 MILKKVYXXXXXXXXXXXXRTVDSGXSKQLHIKLPDEDDDYLAKLIGVFNDICTYSINNK 60 M+LK+VY + D+G SK LHIKLP+EDDDYL KLI +FN+ICTYS+NNK Sbjct: 47 MVLKRVYLFVVFILFYISIQYADAG*SKTLHIKLPNEDDDYLGKLINIFNNICTYSLNNK 226 Query: 61 EKIAKILSTSAVSVYTITSLYNSGITFKKNPHYSFFLPSQKYXXXXXXXXXXXXXXXXXX 120 +KIAKI++TS +S YT+ SLYNSGITFKKNPHYS F PS +Y Sbjct: 227 KKIAKIITTSLLSGYTLNSLYNSGITFKKNPHYSLFFPSNEYIKNVFKKLKKNYEIKTKL 406 Query: 121 XXXXXXXPIFER--TFTNKHNIQMSKTYXXXXXXXXXXXXPYKLKKESLYNFSKYKEFDG 178 IFER T+ + +I + Y P KLK SLYNFSKY+EF Sbjct: 407 KKDQ----IFERDITWYDNQSINNVEIYVSNNLKNFLSFFPNKLKLTSLYNFSKYEEFKD 574 Query: 179 LKYVENPFTQVQVLNHEDNIIVYQAKTKKHYWIHLPFKYNIIKISDDNTTSYILMFIPLY 238 LK N F V+V+ H+++I ++ K KKH+WIH+P KYNI K +TT+ L IPLY Sbjct: 575 LK---NLFNYVKVMKHQNDIFIFHGKLKKHFWIHIPMKYNITKSETGDTTT--LTIIPLY 739 Query: 239 KYYSNYIIQMKITSNNNNQNVTFSSCIKQEKTEHIQNNSFHYDIIKNISKHITYXXXXXX 298 KYYS+Y+I+++ NN N+ F + IK ++ N ++ +IIKNI+ + TY Sbjct: 740 KYYSDYVIEIQFVKQNN--NMKFITSIKSKENNKKNTNLYYKNIIKNIAMYFTYDLFNGI 913 Query: 299 XXXXXXLYMRNVKPGKYNFINTTNALQXXXXXXXXXXFTLPSFKSLPFKFKR 350 Y R VK K +FI N L + P FK K KR Sbjct: 914 HNNLDIFYKRGVKLRKISFIRPNNLLS--KKNSKGTTYRAPLFKHYKLKTKR 1063 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY02589|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 2511 Score = 30.0 bits (66), Expect = 1.8, Method: Composition-based stats. Identities = 15/38 (39%), Positives = 22/38 (57%) Frame = -3 Query: 201 YQAKTKKHYWIHLPFKYNIIKISDDNTTSYILMFIPLY 238 YQ TK+H HLP Y K + N + YI++F+P + Sbjct: 1912 YQ*HTKRHISTHLPLSYINTKSNSYNLSKYIILFVPFH 1799 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY01965|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 2794 Score = 29.3 bits (64), Expect = 3.0, Method: Composition-based stats. Identities = 16/50 (32%), Positives = 28/50 (56%) Frame = -3 Query: 212 HLPFKYNIIKISDDNTTSYILMFIPLYKYYSNYIIQMKITSNNNNQNVTF 261 HLP Y K + + YI +F+P Y ++ +YI ++ I N+N + T+ Sbjct: 1763 HLPLSYINKKCNSYILSKYITLFVPFYPHFFSYIFRLNINFKNSNLSYTY 1614 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00824|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 11370 Score = 27.7 bits (60), Expect = 9.0, Method: Composition-based stats. Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = -1 Query: 211 IHLPFKYNIIKISDDNTTSYILMFIPLY 238 IH PFKYN + I++D S + F+ L+ Sbjct: 2952 IHFPFKYNSLSITEDVCISVSVAFVLLF 2869 Database: genome.fa Posted date: Feb 25, 2008 2:31 PM Number of letters in database: 20,171,213 Number of sequences in database: 2960 Lambda K H 0.322 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2960 Number of Hits to DB: 10,319,596 Number of extensions: 234385 Number of successful extensions: 2631 Number of sequences better than 10.0: 133 Number of HSP's gapped: 2618 Number of HSP's successfully gapped: 141 Length of query: 351 Length of database: 6,723,737 Length adjustment: 98 Effective length of query: 253 Effective length of database: 6,433,657 Effective search space: 1627715221 Effective search space used: 1627715221 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 44 (21.6 bits)