TBLASTN 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= PF14_0033_changed | Plasmodium falciparum 3D7 | conserved Plasmodium selenoprotein, unknown function|Sel1 protein | protein | length=114 (116 letters) Database: genome.fa 2960 sequences; 20,171,213 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_yoelii_yoelii_str._17XNL|MALPY02298|2002-09-10|ds-DNA... 37 0.001 Plasmodium_yoelii_yoelii_str._17XNL|MALPY02422|2002-09-10|ds-DNA... 26 3.4 Plasmodium_yoelii_yoelii_str._17XNL|MALPY00234|2002-09-10|ds-DNA... 25 8.6 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY02298|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 3736 Score = 37.4 bits (85), Expect = 0.001, Method: Composition-based stats. Identities = 17/34 (50%), Positives = 26/34 (76%) Frame = +2 Query: 7 NKKNADYEIFLRDRKLKYEKIRKRNALHNKFILP 40 N + DYE L++RKLKYE +R+ N+ +NK+I+P Sbjct: 2825 NDEKNDYESILKNRKLKYENLRRNNSSYNKYIVP 2926 Score = 35.4 bits (80), Expect = 0.005, Method: Composition-based stats. Identities = 16/25 (64%), Positives = 20/25 (80%) Frame = +1 Query: 92 RTGGLPKSRIMELKNLGTCLGSTXG 116 +TG + +SRIMELKN+ CLGST G Sbjct: 3403 KTGNIGRSRIMELKNISACLGST*G 3477 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY02422|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 4686 Score = 26.2 bits (56), Expect = 3.4, Method: Composition-based stats. Identities = 18/63 (28%), Positives = 25/63 (39%) Frame = +1 Query: 3 DRKENKKNADYEIFLRDRKLKYEKIRKRNALHNKFILPXXXXXXXXXXXXXTVINILAIF 62 ++ +N +NA +F KY+ I + N F P INIL F Sbjct: 340 NKSDNNENAFMNLF------KYKNIISYDIFINSFSYPNDSSFQAIVHFSHVFINILRTF 501 Query: 63 FKT 65 FKT Sbjct: 502 FKT 510 >Plasmodium_yoelii_yoelii_str._17XNL|MALPY00234|2002-09-10|ds- DNA|Plasmodium_yoelii_TIGR Length = 6499 Score = 24.6 bits (52), Expect = 8.6, Method: Composition-based stats. Identities = 16/33 (48%), Positives = 17/33 (51%) Frame = +1 Query: 7 NKKNADYEIFLRDRKLKYEKIRKRNALHNKFIL 39 N KN + I DRKLK EK LHN IL Sbjct: 5251 NVKNINKNIQKVDRKLKKEKYAYDALLHNSLIL 5349 Database: genome.fa Posted date: Feb 25, 2008 2:31 PM Number of letters in database: 20,171,213 Number of sequences in database: 2960 Lambda K H 0.325 0.141 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2960 Number of Hits to DB: 2,203,361 Number of extensions: 25546 Number of successful extensions: 240 Number of sequences better than 10.0: 12 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 13 Length of query: 116 Length of database: 6,723,737 Length adjustment: 83 Effective length of query: 33 Effective length of database: 6,478,057 Effective search space: 213775881 Effective search space used: 213775881 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 40 (20.0 bits)