TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000032_1.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Homo sapiens
         (392 letters)

Database: genome.fa 
           2809 sequences; 26,957,458 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_viv...    46   3e-05
Plasmodium_vivax_SaI-1|ctg_7165|2005-09-01|ds-DNA|Plasmodium_viv...    29   4.8  

>Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR
          Length = 1198945

 Score = 46.2 bits (108), Expect = 3e-05,   Method: Composition-based stats.
 Identities = 46/169 (27%), Positives = 75/169 (44%), Gaps = 6/169 (3%)
 Frame = -1

Query: 2      STRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVM 61
              + RE  N  SY  +   ++       G G KVP +VL   L+ L    +     +LG   
Sbjct: 402439 AKREKVN--SYIKESIEKIINRNTCGGCGSKVPSNVLSNSLKGLP--MYSSPNVYLGIE- 402275

Query: 62     PRLGIGMDTCVIPL----RHGGLS--LVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMG 115
                     D C I +    + G  S  LVQT D+    +DD Y++G I   + LSD+Y+MG
Sbjct: 402274 -----ACDDCCIFVHSKSKKGEESPALVQTIDFFKSFIDDEYILGEIIAIHCLSDVYSMG 402110

Query: 116    VTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQT 164
               T    + +L+   N   +++  + +  I+ G     +E    ++GG T
Sbjct: 402109 GTGICALCVLIVKDN--IEKKLQQRLQNILTGCCQKLKEEKCVLSGGHT 401969



 Score = 43.1 bits (100), Expect = 3e-04,   Method: Composition-based stats.
 Identities = 28/96 (29%), Positives = 40/96 (41%), Gaps = 1/96 (1%)
 Frame = -1

Query: 194    GDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAA- 252
              GD+++ TK  G    +A H       +W                   +  M   NR +  
Sbjct: 401623 GDIIITTKMFGFGFIMAAHICKKAKARW---------------IYNCLDEMLLSNRKSGL 401489

Query: 253    GLMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSF 288
               L+   NA A TD+TGFG+LGH   + K  R E+ F
Sbjct: 401488 YLLQNNNAKACTDVTGFGVLGHLNEMIKCSRREIYF 401381



 Score = 30.4 bits (67), Expect = 2.1,   Method: Composition-based stats.
 Identities = 16/46 (34%), Positives = 28/46 (60%)
 Frame = -1

Query: 300    AAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKY 345
              A++S+  G +F       P+TSGGL+  + R++A R  A++K+  Y
Sbjct: 401128 ASLSERYGILFD------PQTSGGLMAIVERDRAERILADLKNMGY 401009


>Plasmodium_vivax_SaI-1|ctg_7165|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR
          Length = 54073

 Score = 29.3 bits (64), Expect = 4.8,   Method: Composition-based stats.
 Identities = 18/48 (37%), Positives = 23/48 (47%)
 Frame = -3

Query: 2     STRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENH 49
             S + + NP+ ++ D SF LT      G          Q LLES QENH
Sbjct: 47789 SDKPTNNPQLHQSDSSFVLTYLPSPSG----------QNLLESSQENH 47676


  Database: genome.fa
    Posted date:  Feb 25, 2008  2:49 PM
  Number of letters in database: 26,957,458
  Number of sequences in database:  2809
  
Lambda     K      H
   0.320    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2809
Number of Hits to DB: 11,440,259
Number of extensions: 169979
Number of successful extensions: 563
Number of sequences better than 10.0: 2
Number of HSP's gapped: 563
Number of HSP's successfully gapped: 4
Length of query: 392
Length of database: 8,985,819
Length adjustment: 101
Effective length of query: 291
Effective length of database: 8,702,110
Effective search space: 2532314010
Effective search space used: 2532314010
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SPP00000032_1.0 # Protein # Selenophosphate synthetase 1 (SPS1) # Homo sapiens
# Database: genome.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SPP00000032_1.0	Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	27.22	169	117	6	2	164	402439	401969	3e-05	46.2
SPP00000032_1.0	Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	29.17	96	67	2	194	288	401623	401381	3e-04	43.1
SPP00000032_1.0	Plasmodium_vivax_SaI-1|ctg_7052|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	34.78	46	30	1	300	345	401128	401009	2.1	30.4
SPP00000032_1.0	Plasmodium_vivax_SaI-1|ctg_7165|2005-09-01|ds-DNA|Plasmodium_vivax_TIGR	37.50	48	30	1	2	49	47789	47676	4.8	29.3