TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= SPP00000038_1.0 # Protein # Eukaryotic elongation factor
(eEFSec) # Homo sapiens # Complete
(596 letters)
Database: genoma.fasta
14 sequences; 23,462,190 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-DNA|Plasmodium_... 206 3e-53
Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|... 127 2e-29
Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmod... 92 1e-18
Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds... 72 7e-13
Plasmodium_knowlesi_strain_H|PK4.chr05|2007-02-22|ds-DNA|Plasmod... 59 1e-08
Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmod... 57 3e-08
Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_... 50 5e-06
Plasmodium_knowlesi_strain_H|chr10|2007-02-22|ds-DNA|Plasmodium_... 45 9e-05
Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmod... 33 0.42
Plasmodium_knowlesi_strain_H|PK4.chr02|2007-02-22|ds-DNA|Plasmod... 32 1.1
>Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 726886
Score = 206 bits (525), Expect = 3e-53, Method: Composition-based stats.
Identities = 191/696 (27%), Positives = 294/696 (42%), Gaps = 164/696 (23%)
Frame = +1
Query: 6 VNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLR 65
+NVNVGVLGH+DSGKT+L R LS ST A DK QS+E+GIT+DLGFS F + R R
Sbjct: 167698 INVNVGVLGHVDSGKTSLCRCLSEVLSTCALDKHRQSQEKGITIDLGFSSFYLRRRKRNR 167877
Query: 66 SS---------------LP--------------------------------EFQAAPEAE 78
+ +P E A P
Sbjct: 167878 DAEGAEGGHNGQITSREIPNGKVFHNELFEEEHSDGRVCNGSACGGEVPSGEGSADPLNC 168057
Query: 79 PEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIAC 138
E LQ+ LVDCPGH SL+ II GA+I D+++L+ID+TKG+Q Q+ ECLV+ ++
Sbjct: 168058 SSAEEETLQICLVDCPGHHSLLNCIIMGAEITDMILLLIDITKGIQKQTVECLVLCEVLQ 168237
Query: 139 QKLVVVLNKIDLLPEGKRQAAIDKMTKKMQ------KTLENTKFRGAPIIPVAA--KPGG 190
+ +VVVLNKIDL+P +R+ I M +K++ L N K+ ++ ++A K G
Sbjct: 168238 RDVVVVLNKIDLVPAEQRERRIKLMKEKIELVFRSKAGLRNLKWY---VVGLSAQLKKGE 168408
Query: 191 PEA------------------------PETEAPQGI----------PELIELLTSQISIP 216
P A P + A GI +LI LL I +P
Sbjct: 168409 PTAGAYPPNSLPPTDSKLQSQSQWQCRPVSNADDGIGASPPRSENVAQLINLLREIIRVP 168588
Query: 217 TRDPSGP--------------------FLMSVDHCFSIKGQGTVMTGTILSGSISLGDSV 256
TR F DH F IKG+G TGT++ G+I +V
Sbjct: 168589 TRRGKNSHNGGGDDNNDDDGNDRGGDEFYFLYDHTFDIKGKGRAYTGTVIRGTIKRNATV 168768
Query: 257 EIPALKVVKKVKSMQMFHMPITSAMQGDRLGICV----TQFDPKLLERGLVCAPESLHTV 312
I +K KVK +Q F + QG+RL + + + K +ERG++ + E +
Sbjct: 168769 SILPMKEKGKVKEIQSFKQIVNEGTQGNRLSLLICNDHIRNSGKKIERGVIVS-EISNIA 168945
Query: 313 HAALI--SVEKIPYFRGPLQTKAKFHITVGHETVMGRLMFFSPAPDNFDQEPIL------ 364
H ++ V+ + Y+ + + +G T FF P + P
Sbjct: 168946 HFSIFICRVKLVQYYGRSISSSELLSCVIGFSTSPCYGYFFRPINKDTGGMPSQRGRYLK 169125
Query: 365 ----------DSFNFSQEYLFQEQYL---SKDLTPAVTDNDEADKKAGQATEGHCPRQQW 411
+F+ +YLF Q + + +N ++D + +
Sbjct: 169126 QTSPTKACEKKTFDPQGDYLFINQIIFEQEEQKKKESEENPKSDD------------EFF 169269
Query: 412 ALVEFEKPVTCPR--LCLVIGSRLDADIHTNTCRLAFHGILLHGLEDRNYADSFLP---- 465
LV +K + C + C+ I + + + CR+ HGI + + D Y P
Sbjct: 169270 FLVILKKKIYCYKKEKCIFIKNEENLN-----CRICLHGI-IEDIVDDGYPHQKTPFIAG 169431
Query: 466 ---------RLKVYKL---KHKHGLVERAMDDYSVIGRSLFKKETNIQLFVGLKVHLSTG 513
R K +K+ K K G +ER DD++V+ R++F+ T + ++G ++ L
Sbjct: 169432 KKPTLSDFSRFKNFKMLIEKQKRGEIERVQDDHTVVCRNMFRCSTQVAPYLGKEICLVDA 169611
Query: 514 E------------LGIIDSAFGQSGKFKIHIPGGLS 537
+ +G+I + F +SGKF H LS
Sbjct: 169612 DYKSDLRDVEVRHIGVITAPFAKSGKFIAHFQEDLS 169719
>Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 3128370
Score = 127 bits (319), Expect = 2e-29, Method: Composition-based stats.
Identities = 87/316 (27%), Positives = 155/316 (49%), Gaps = 16/316 (5%)
Frame = +2
Query: 4 RRVNVNVGVLGHIDSGKTALARALS---------TTASTAAFDKQPQSRERGITLDLGFS 54
++ ++N+G +GH+D GKT L A++ T S DK P+ ++RGIT++
Sbjct: 912149 KKPHMNIGTIGHVDHGKTTLTAAITKVCASLKRATFKSYEEIDKTPEEQKRGITIN---- 912316
Query: 55 CFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMM 114
++ E++ + +DCPGH ++ +I G +D +
Sbjct: 912317 -----------ATHVEYETEKR----------HYSHIDCPGHLDYVKNMITGTSQMDGSI 912433
Query: 115 LVIDVTKGMQTQSAECLVIG-QIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLEN 173
LV+ G+ Q+ E +++ QI +K++V LNKID+ + + +D + ++++ L
Sbjct: 912434 LVVSAYDGLMPQTKEHVLLSRQIGIEKMIVYLNKIDMCED---KELVDLVELEIRELLSF 912604
Query: 174 TKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTS---QISIPTRDPSGPFLMSVDH 230
K+ G I P + ++ G+P +++LL + I P R PFLMS+D
Sbjct: 912605 HKYDGDNI-PFIKGSALKALNDDQSEYGVPSILKLLDACDNYIDEPKRKMDLPFLMSIDD 912781
Query: 231 CFSIKGQGTVMTGTILSGSISLGDSVEIPALK---VVKKVKSMQMFHMPITSAMQGDRLG 287
I G+GTV TG + G++ L DSVEI +K + V ++MF + +A GD++G
Sbjct: 912782 VLQISGKGTVATGRVEQGTLKLNDSVEIMGIKEKPIKTVVTGIEMFRKTLDAAQAGDQIG 912961
Query: 288 ICVTQFDPKLLERGLV 303
+ + L RG+V
Sbjct: 912962 VMLKNVKRNDLTRGMV 913009
Score = 43.9 bits (102), Expect = 3e-04, Method: Composition-based stats.
Identities = 36/167 (21%), Positives = 72/167 (43%), Gaps = 2/167 (1%)
Frame = -1
Query: 9 NVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPARLRSSL 68
N+ V+ H+D GK+ L +L + A + +R D C ++ +S+
Sbjct: 2388174 NMSVIAHVDHGKSTLTDSLVSKAGIISSKHAGDARFTDTRADEQERCITI------KSTG 2388013
Query: 69 PEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSA 128
+ E G+ + L+D PGH + ++ D ++V+D +G+ Q+
Sbjct: 2388012 ISMYFEHDLEDGEGKKPFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTE 2387833
Query: 129 ECLVIGQIACQKLVVVLNKID--LLPEGKRQAAIDKMTKKMQKTLEN 173
L K V+ +NK+D LL + Q ++ + + +T+E+
Sbjct: 2387832 TVLYQALGERIKPVLHVNKVDRALL---ELQMEVEDIYQTFARTIES 2387701
>Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2200295
Score = 91.7 bits (226), Expect = 1e-18, Method: Composition-based stats.
Identities = 82/277 (29%), Positives = 134/277 (48%), Gaps = 22/277 (7%)
Frame = -3
Query: 4 RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPAR 63
R+ +N+G +GH+ GK+ L A+S T F + +ER IT+ LG++ +
Sbjct: 1776921 RQATINLGTIGHVAHGKSTLVHAISGV-HTVRFKHE---KERNITIKLGYANAKIYKCTN 1776754
Query: 64 LRSSLPE----FQAAPEAEPE-PGEP-------LLQVTLVDCPGHASLIRTIIGGAQIID 111
PE ++++ E +P P E L V+ VDCPGH L+ T++ GA ++D
Sbjct: 1776753 PDCLPPECYKSYESSKEDDPMCPRENCNHKMKLLRHVSFVDCPGHDILMATMLNGAAVMD 1776574
Query: 112 LMMLVIDVTKGM-QTQSAECLVIGQIACQKLVVVL-NKIDLLPEGKRQAAIDKMTKKMQK 169
+L++ + Q Q++E L +I K +++L NK++L+ E QA K ++++
Sbjct: 1776573 AALLLVAGNESCPQPQTSEHLAAVEIMRLKHILILQNKVELIKE--EQAL--KQQEEIRN 1776406
Query: 170 TLENTKFRGAPIIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVD 229
+ T APIIP++A I + E + +QISIP RD M V
Sbjct: 1776405 FVSGTAADSAPIIPISAV----------LKYNIDVVCEYIVTQISIPKRDFISSPHMIVI 1776256
Query: 230 HCFSIKG--------QGTVMTGTILSGSISLGDSVEI 258
F + QG V G+IL G + +GD +EI
Sbjct: 1776255 RSFDVNKPGEDIETLQGGVAGGSILHGVLKVGDKIEI 1776145
>Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2372884
Score = 72.4 bits (176), Expect = 7e-13, Method: Composition-based stats.
Identities = 78/342 (22%), Positives = 141/342 (41%), Gaps = 37/342 (10%)
Frame = +3
Query: 1 MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
M + ++N+ V+GH+DSGK+ R + +A
Sbjct: 657075 MGKEKTHINLVVIGHVDSGKSTTTGHIIYKLGGIDRRTIEKFEKESAEMGKGSFKYAWVL 657254
Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
DK RERGIT+D+ F P T++D PGH
Sbjct: 657255 DKLKAERERGITIDIALWKFETPR-------------------------YFFTVIDAPGH 657359
Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
I+ +I G D+ +LV+ G + Q+ E L+ + +++VV +NK+
Sbjct: 657360 KDFIKNMITGTSQADVALLVVPAEVGGFEGAFSKEGQTKEHALLAFTLGVKQIVVGVNKM 657539
Query: 149 DLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPI--IPVAAKPGG---PEAPETEAPQGIP 203
D + + + +++ K+++ L+ ++ + IP++ G ++ +T +G
Sbjct: 657540 DTVKYSEDR--YEEIKKEVKDYLKKVGYQADKVDFIPISGFEGDNLIEKSDKTPWYKG-R 657710
Query: 204 ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263
LIE L + + P R P + + + I G GTV G + +G + G + V
Sbjct: 657711 TLIEALDT-MEPPKRPYDKPLRIPLQGVYKIGGIGTVPVGRVETGILKAGMVLNFAPSAV 657887
Query: 264 VKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
V + KS++M + A GD +G V K ++RG V +
Sbjct: 657888 VSECKSVEMHKEVLEEARPGDNIGFNVKNVSVKEIKRGYVAS 658013
Score = 72.4 bits (176), Expect = 7e-13, Method: Composition-based stats.
Identities = 78/342 (22%), Positives = 141/342 (41%), Gaps = 37/342 (10%)
Frame = -2
Query: 1 MAGRRVNVNVGVLGHIDSGKTALA------------RALSTTASTAA------------F 36
M + ++N+ V+GH+DSGK+ R + +A
Sbjct: 655569 MGKEKTHINLVVIGHVDSGKSTTTGHIIYKLGGIDRRTIEKFEKESAEMGKGSFKYAWVL 655390
Query: 37 DKQPQSRERGITLDLGFSCFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGH 96
DK RERGIT+D+ F P T++D PGH
Sbjct: 655389 DKLKAERERGITIDIALWKFETPR-------------------------YFFTVIDAPGH 655285
Query: 97 ASLIRTIIGGAQIIDLMMLVIDVTKG-------MQTQSAE-CLVIGQIACQKLVVVLNKI 148
I+ +I G D+ +LV+ G + Q+ E L+ + +++VV +NK+
Sbjct: 655284 KDFIKNMITGTSQADVALLVVPAEVGGFEGAFSKEGQTKEHALLAFTLGVKQIVVGVNKM 655105
Query: 149 DLLPEGKRQAAIDKMTKKMQKTLENTKFRGAPI--IPVAAKPGG---PEAPETEAPQGIP 203
D + + + +++ K+++ L+ ++ + IP++ G ++ +T +G
Sbjct: 655104 DTVKYSEDR--YEEIKKEVKDYLKKVGYQADKVDFIPISGFEGDNLIEKSDKTPWYKG-R 654934
Query: 204 ELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTVMTGTILSGSISLGDSVEIPALKV 263
LIE L + + P R P + + + I G GTV G + +G + G + V
Sbjct: 654933 TLIEALDT-MEPPKRPYDKPLRIPLQGVYKIGGIGTVPVGRVETGILKAGMVLNFAPSAV 654757
Query: 264 VKKVKSMQMFHMPITSAMQGDRLGICVTQFDPKLLERGLVCA 305
V + KS++M + A GD +G V K ++RG V +
Sbjct: 654756 VSECKSVEMHKEVLEEARPGDNIGFNVKNVSVKEIKRGYVAS 654631
Score = 50.1 bits (118), Expect = 3e-06, Method: Composition-based stats.
Identities = 42/143 (29%), Positives = 67/143 (46%), Gaps = 1/143 (0%)
Frame = -2
Query: 10 VGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERG-ITLDLGFSCFSVPLPARLRSSL 68
V +LGH+D+GKT L L T Q E G IT +G + F + +
Sbjct: 2040144 VCILGHVDTGKTKLLDKLRHT--------NVQDNEAGGITQQIGATFFPKDVLDK----- 2040004
Query: 69 PEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSA 128
E + E+ + ++ ++D PGH S G+ + D+ +LVID+ G++ Q+
Sbjct: 2040003 -EIKKVDESIKCMSKGIM---IIDTPGHESFYNLRKRGSSLCDIAILVIDLMHGLEQQTK 2039836
Query: 129 ECLVIGQIACQKLVVVLNKIDLL 151
E + I + V+ LNKID L
Sbjct: 2039835 ESIQILKQRNCPFVIALNKIDRL 2039767
>Plasmodium_knowlesi_strain_H|PK4.chr05|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1324984
Score = 58.5 bits (140), Expect = 1e-08, Method: Composition-based stats.
Identities = 51/187 (27%), Positives = 83/187 (44%), Gaps = 3/187 (1%)
Frame = -1
Query: 4 RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPAR 63
++ N V +LGH+D GKT L + Q+ GIT LG F V
Sbjct: 212050 KKRNPVVTILGHVDHGKTTLLDRFRNS-------NVAQNEIGGITQKLG--AFEV----- 211913
Query: 64 LRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM 123
+ E G ++T +D PGH+ + AQ DL++LVI + G+
Sbjct: 211912 -------------VDKETGR---KITFLDTPGHSVFKKIRQRCAQCTDLIILVISIDDGI 211781
Query: 124 QTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFR---GAP 180
+++ EC+ + Q L++ NKID K + ++K++K + T+ P
Sbjct: 211780 MSETVECMKLAQKFNIPLIIAANKID-----KCGSDLEKLSKSLVAYDVITELEENGNVP 211616
Query: 181 IIPVAAK 187
IIP++AK
Sbjct: 211615 IIPISAK 211595
>Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1496036
Score = 57.4 bits (137), Expect = 3e-08, Method: Composition-based stats.
Identities = 68/287 (23%), Positives = 113/287 (39%), Gaps = 11/287 (3%)
Frame = +3
Query: 9 NVGVLGHIDSGKTALA-RALSTTASTAAFDKQPQ-------SRERGITLDLGFSCFSVPL 60
N +L HIDSGK+ LA R L T + Q Q RE+GIT+ L
Sbjct: 466218 NFCILAHIDSGKSTLADRFLELTKTIKKKKMQDQFLDMMSLEREKGITIKL--------- 466370
Query: 61 PARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVT 120
+R + + L+D PGH + + + +L+ID T
Sbjct: 466371 -KAVRMNYKNYI---------------FNLIDTPGHFDFYHEVKRSLSVCEGAILLIDGT 466502
Query: 121 KGMQTQSAECLVIGQIACQKLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLENTKFRGAP 180
KG+Q+Q+ + Q K++ V+NKIDL I ++ K L F
Sbjct: 466503 KGIQSQTLNIFMELQKHNLKIIPVINKIDL--------NICRLEKIENDLLNKFHFMKKD 466658
Query: 181 IIPVAAKPGGPEAPETEAPQGIPELIELLTSQISIPTRDPSGPFLMSVDHCFSIKGQGTV 240
I+ ++AK G+ L + + S+I P + F V F + +G +
Sbjct: 466659 ILHISAK----------YSHGVESLFQRIVSEIPSPAIKSNSFFRAIVFDSFYDQYKGVI 466808
Query: 241 MTGTILSGSISLGDSV---EIPALKVVKKVKSMQMFHMPITSAMQGD 284
+ +L+G ++ V + ++++V + P S QGD
Sbjct: 466809 LIIKVLNGVLTKKTEVFFIQSEKTYIIQEVGYLTPEMKPTESIRQGD 466949
Score = 32.0 bits (71), Expect = 1.1, Method: Composition-based stats.
Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 19/61 (31%)
Frame = -2
Query: 8 VNVGVLGHIDSGKTALARAL-------------------STTASTAAFDKQPQSRERGIT 48
+N+ VLGHID+GK+ L AL ++ T D++ RER IT
Sbjct: 1315045 LNILVLGHIDAGKSTLIGALLYNLSYVSEQTVKKYEHVRESSKYTFILDEEDDERERNIT 1314866
Query: 49 L 49
L
Sbjct: 1314865 L 1314863
Score = 30.0 bits (66), Expect = 4.0, Method: Composition-based stats.
Identities = 16/71 (22%), Positives = 33/71 (46%), Gaps = 4/71 (5%)
Frame = -2
Query: 85 LLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECL----VIGQIACQK 140
L ++ + D PGH L+ + + D +LV+D ++ E ++ +
Sbjct: 1314634 LRKINIFDTPGHNELVTNLHTWSFFADSAILVVDANNIYNQKNDETYRNISILEAVGISN 1314455
Query: 141 LVVVLNKIDLL 151
++V +NK+DL
Sbjct: 1314454 VIVAVNKLDLF 1314422
>Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 3159096
Score = 49.7 bits (117), Expect = 5e-06, Method: Composition-based stats.
Identities = 33/131 (25%), Positives = 52/131 (39%), Gaps = 11/131 (8%)
Frame = -2
Query: 8 VNVGVLGHIDSGKTALARAL-----------STTASTAAFDKQPQSRERGITLDLGFSCF 56
+N+G+L HID+GKT ++ + S D Q RERGIT+ +SCF
Sbjct: 2445479 MNLGILAHIDAGKTTISEDILYNANEIKVKGSINDQNTQLDFLRQERERGITIKTAYSCF 2445300
Query: 57 SVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLV 116
++V L+D PGH + D ++V
Sbjct: 2445299 K-------------------------WNNVKVNLIDTPGHVDFSNETFLSLCVSDKCVIV 2445195
Query: 117 IDVTKGMQTQS 127
+D +G+Q Q+
Sbjct: 2445194 VDAKEGIQIQT 2445162
Score = 41.2 bits (95), Expect = 0.002, Method: Composition-based stats.
Identities = 61/266 (22%), Positives = 106/266 (39%), Gaps = 18/266 (6%)
Frame = +3
Query: 9 NVGVLGHIDSGKTALARAL-----STTASTAAFDKQPQSRERGITLDLGFSCFSVPLPAR 63
NV ++ H+D GKT L L T + D +ERGIT+ + R
Sbjct: 1011243 NVAIIAHVDHGKTTLVDKLLKQGGEETKNERVMDHNDLEKERGITI--------MSKVTR 1011398
Query: 64 LRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM 123
++ F +VD PGH+ + +ID + L+IDV +G
Sbjct: 1011399 IKYDDYFFN-----------------IVDTPGHSDFGGEVERVLNLIDGVCLIIDVVEGP 1011527
Query: 124 QTQSAECLVIGQIACQ-KLVVVLNKIDLLPEGKRQAAIDKMTKKMQKTLEN-TKFRGAPI 181
+ Q+ L + + K++V++NK D K + I+ + L + PI
Sbjct: 1011528 KNQTKFVLKKSLLNPKCKIIVIMNKFDKPSNIKSKEEIENEIFDLFADLNAPDELMNYPI 1011707
Query: 182 IPVAAKPGGPEAPETEAPQG----------IPELIELLTSQISIPTRDPSGPFLMSVDHC 231
+ +AK G +A Q ++IE S + +P+ + SVD
Sbjct: 1011708 LYASAKNGWCTDNFEDAKQNKCEHNNVLAIFKKIIEYFDSPMMLPSGSED---VSSVDS- 1011875
Query: 232 FSIKGQGTVMTGTILSG-SISLGDSV 256
+ Q + +TG +G + ++G+SV
Sbjct: 1011876 -KMGSQNSFITGDDANGITTAVGESV 1011950
Score = 38.9 bits (89), Expect = 0.008, Method: Composition-based stats.
Identities = 32/133 (24%), Positives = 49/133 (36%), Gaps = 14/133 (10%)
Frame = -2
Query: 9 NVGVLGHIDSGKTALA-RALSTTAST-------------AAFDKQPQSRERGITLDLGFS 54
N+G+ HID+GKT L R L T A D RE+GIT+
Sbjct: 2249243 NIGISAHIDAGKTTLTERILYYTGKIKSIHEVRGSDGVGATMDSMDLEREKGITIQ---- 2249076
Query: 55 CFSVPLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMM 114
AA + + ++D PGH + +++D +
Sbjct: 2249075 -----------------SAATNCNWDFNNKKFTINIIDTPGHVDFTIEVERSLRVLDAAV 2248947
Query: 115 LVIDVTKGMQTQS 127
LVI G+Q+Q+
Sbjct: 2248946 LVICGVSGVQSQT 2248908
>Plasmodium_knowlesi_strain_H|chr10|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1486039
Score = 45.4 bits (106), Expect = 9e-05, Method: Composition-based stats.
Identities = 37/147 (25%), Positives = 68/147 (46%)
Frame = +2
Query: 4 RRVNVNVGVLGHIDSGKTALARALSTTASTAAFDKQPQSRERGITLDLGFSCFSVPLPAR 63
++ N+ V +GHI+ GKT+L + T + +E+G+ + F V
Sbjct: 779606 KKRNIVVTFIGHINHGKTSLFDYICKT--------NERDKEKGL-ITQNIRAFKV----- 779743
Query: 64 LRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGM 123
+S +F TL+D PGH + + G +I D+ +LVI +G+
Sbjct: 779744 -KSRNNDFT---------------FTLIDTPGHEAFMPIRSRGVKISDVSILVISGEEGI 779875
Query: 124 QTQSAECLVIGQIACQKLVVVLNKIDL 150
Q Q+ EC+ + + ++V+ + K+DL
Sbjct: 779876 QEQTVECIKLIKEHDIRIVIAVTKMDL 779956
>Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2147124
Score = 33.1 bits (74), Expect = 0.42, Method: Composition-based stats.
Identities = 30/124 (24%), Positives = 56/124 (45%), Gaps = 6/124 (4%)
Frame = +1
Query: 5 RVNVNVGVLGHIDSGK-TALARALSTTA-----STAAFDKQPQSRERGITLDLGFSCFSV 58
R ++N+ +GH+D+GK TA L + ++++ + + R + F F +
Sbjct: 927496 RQHLNIIFIGHVDAGKSTACGNILYILGYVDDRTIEKYEREAKEKNR----ESWFLAFIM 927663
Query: 59 PLPARLRSSLPEFQAAPEAEPEPGEPLLQVTLVDCPGHASLIRTIIGGAQIIDLMMLVID 118
+ R + A E + + T++D PGH + I +I GA D+ +L+I
Sbjct: 927664 DINEEERQKGKTVEVG-RAHFETKDR--RFTILDAPGHKNFIPNMISGAAQADIGVLIIS 927834
Query: 119 VTKG 122
KG
Sbjct: 927835 ARKG 927846
>Plasmodium_knowlesi_strain_H|PK4.chr02|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1770351
Score = 32.0 bits (71), Expect = 1.1, Method: Composition-based stats.
Identities = 22/90 (24%), Positives = 39/90 (43%), Gaps = 6/90 (6%)
Frame = -3
Query: 90 LVDCPGHASLIRTIIGGAQIIDLMMLVIDVTKGMQTQSAECLVIGQIACQKLVVVLNKID 149
++D PGH + + I + LV+DVT G + + K+V++LN ID
Sbjct: 152773 IMDTPGHVNFFDEFLCAVNICECCCLVVDVTDGCMYVTENVIKTCIYENVKIVLILNCID 152594
Query: 150 ------LLPEGKRQAAIDKMTKKMQKTLEN 173
LP I+ +++ K +E+
Sbjct: 152593 KLIMDLRLPPNDAYHKINYTIEEINKKIES 152504
Database: genoma.fasta
Posted date: Mar 3, 2008 9:06 AM
Number of letters in database: 23,462,190
Number of sequences in database: 14
Lambda K H
0.319 0.136 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 14,981,766
Number of extensions: 228968
Number of successful extensions: 926
Number of sequences better than 10.0: 10
Number of HSP's gapped: 914
Number of HSP's successfully gapped: 24
Length of query: 596
Length of database: 7,820,730
Length adjustment: 104
Effective length of query: 492
Effective length of database: 7,819,274
Effective search space: 3847082808
Effective search space used: 3847082808
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SPP00000038_1.0 # Protein # Eukaryotic elongation factor (eEFSec) # Homo sapiens # Complete
# Database: genoma.fasta
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 27.44 696 341 20 6 537 167698 169719 3e-53 206
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 27.53 316 213 8 4 303 912149 913009 2e-29 127
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 21.56 167 129 3 9 173 2388174 2387701 3e-04 43.9
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 29.60 277 173 11 4 258 1776921 1776145 1e-18 91.7
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 22.81 342 227 10 1 305 657075 658013 7e-13 72.4
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 22.81 342 227 10 1 305 655569 654631 7e-13 72.4
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 29.37 143 100 4 10 151 2040144 2039767 3e-06 50.1
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr05|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 27.27 187 133 6 4 187 212050 211595 1e-08 58.5
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 23.69 287 208 7 9 284 466218 466949 3e-08 57.4
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 32.79 61 22 1 8 49 1315045 1314863 1.1 32.0
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 22.54 71 51 1 85 151 1314634 1314422 4.0 30.0
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 25.19 131 87 2 8 127 2445479 2445162 5e-06 49.7
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 22.93 266 187 9 9 256 1011243 1011950 0.002 41.2
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 24.06 133 87 3 9 127 2249243 2248908 0.008 38.9
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|chr10|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 25.17 147 110 4 4 150 779606 779956 9e-05 45.4
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 24.19 124 88 5 5 122 927496 927846 0.42 33.1
SPP00000038_1.0 Plasmodium_knowlesi_strain_H|PK4.chr02|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 24.44 90 62 1 90 173 152773 152504 1.1 32.0
