TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= subseq(908011,5472).[2965:4503].sp.tr
(529 letters)
Database: cds.fasta
5106 sequences; 11,140,562 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_072100|Annotation|Pla... 1077 0.0
Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_132920|Annotation|Pla... 207 6e-54
Plasmodium_knowlesi_strain_H|PK4.chr05|PKH_051660|Annotation|Pla... 108 4e-24
Plasmodium_knowlesi_strain_H|chr14|PKH_144930|Annotation|Plasmod... 97 7e-21
Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_133040|Annotation|Pla... 29 3.5
Plasmodium_knowlesi_strain_H|PK4.chr09|PKH_093890|Annotation|Pla... 28 5.2
Plasmodium_knowlesi_strain_H|PK4.chr03|PKH_031670|Annotation|Pla... 28 5.7
Plasmodium_knowlesi_strain_H|PK4.chr05|PKH_052140|Annotation|Pla... 27 8.3
Plasmodium_knowlesi_strain_H|PK4.chr08|PKH_082970|Annotation|Pla... 27 9.9
>Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_072100|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) thioredoxin
reductase 2, putative
Length = 1872
Score = 1077 bits (2786), Expect = 0.0, Method: Composition-based stats.
Identities = 522/522 (100%), Positives = 522/522 (100%)
Frame = +1
Query: 1 MEGKNALEKGGTHPNQSNNHECKSQGECNYDYDYVVIGGGPGGMASAKEAASHGAKVLLF 60
MEGKNALEKGGTHPNQSNNHECKSQGECNYDYDYVVIGGGPGGMASAKEAASHGAKVLLF
Sbjct: 274 MEGKNALEKGGTHPNQSNNHECKSQGECNYDYDYVVIGGGPGGMASAKEAASHGAKVLLF 453
Query: 61 DYVKPSNKGTKWGIGGTCVNVGCVPKKLMHYAGNMGSLFKMDSNPYGWSYNNLIHNWGKL 120
DYVKPSNKGTKWGIGGTCVNVGCVPKKLMHYAGNMGSLFKMDSNPYGWSYNNLIHNWGKL
Sbjct: 454 DYVKPSNKGTKWGIGGTCVNVGCVPKKLMHYAGNMGSLFKMDSNPYGWSYNNLIHNWGKL 633
Query: 121 VTTVQSHIRSLNFSYMTGLRSSKVKYMNGLASLKDEHTVSYYLKGDMSKEELVTAKYILI 180
VTTVQSHIRSLNFSYMTGLRSSKVKYMNGLASLKDEHTVSYYLKGDMSKEELVTAKYILI
Sbjct: 634 VTTVQSHIRSLNFSYMTGLRSSKVKYMNGLASLKDEHTVSYYLKGDMSKEELVTAKYILI 813
Query: 181 ATGCRPSIPDDVEGAKELSITSDDIFSMKKDPGKTLIVGASYVALECAGFLNSLGYDVTV 240
ATGCRPSIPDDVEGAKELSITSDDIFSMKKDPGKTLIVGASYVALECAGFLNSLGYDVTV
Sbjct: 814 ATGCRPSIPDDVEGAKELSITSDDIFSMKKDPGKTLIVGASYVALECAGFLNSLGYDVTV 993
Query: 241 AVRSIVLRGFDSQCALKVKIYMEEQGVLFKHGVLPKKLSKMEGEKISVLFNDGTTELFDT 300
AVRSIVLRGFDSQCALKVKIYMEEQGVLFKHGVLPKKLSKMEGEKISVLFNDGTTELFDT
Sbjct: 994 AVRSIVLRGFDSQCALKVKIYMEEQGVLFKHGVLPKKLSKMEGEKISVLFNDGTTELFDT 1173
Query: 301 VLYATGRKGDIDMLNLDQVKIQVNKNTNKIITNEVSCTNISNIFAVGDVAVDVPELAPVA 360
VLYATGRKGDIDMLNLDQVKIQVNKNTNKIITNEVSCTNISNIFAVGDVAVDVPELAPVA
Sbjct: 1174VLYATGRKGDIDMLNLDQVKIQVNKNTNKIITNEVSCTNISNIFAVGDVAVDVPELAPVA 1353
Query: 361 IKAGEILARRLFNQSEEIMDYTFIPTSIYTPIEYGTCGYSEEKAYEIFGTSNVEVFLQEF 420
IKAGEILARRLFNQSEEIMDYTFIPTSIYTPIEYGTCGYSEEKAYEIFGTSNVEVFLQEF
Sbjct: 1354IKAGEILARRLFNQSEEIMDYTFIPTSIYTPIEYGTCGYSEEKAYEIFGTSNVEVFLQEF 1533
Query: 421 NNLEISAVHREKHVRAQKDQYDTDVSSTCLSKLVCLKNEDNRVVGFHYVGPNAGEITQGM 480
NNLEISAVHREKHVRAQKDQYDTDVSSTCLSKLVCLKNEDNRVVGFHYVGPNAGEITQGM
Sbjct: 1534NNLEISAVHREKHVRAQKDQYDTDVSSTCLSKLVCLKNEDNRVVGFHYVGPNAGEITQGM 1713
Query: 481 ALALRLKAKKSDFDKCIGIHPTDAESFMNLTITRSSGLSFAA 522
ALALRLKAKKSDFDKCIGIHPTDAESFMNLTITRSSGLSFAA
Sbjct: 1714ALALRLKAKKSDFDKCIGIHPTDAESFMNLTITRSSGLSFAA 1839
>Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_132920|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) glutathione
reductase, putative
Length = 1686
Score = 207 bits (526), Expect = 6e-54, Method: Composition-based stats.
Identities = 180/539 (33%), Positives = 271/539 (50%), Gaps = 57/539 (10%)
Frame = +1
Query: 29 NYDYDYVVIGGGPGGMASAKEAASHGAKVLLFDYVKPSNKGTKWGIGGTCVNVGCVPKKL 88
N YD +VIGGG GGMA+A+ AA + AKV L + K +GGTCVNVGCVPKK+
Sbjct: 136 NMVYDLIVIGGGSGGMAAARRAARNKAKVALVE---------KSYLGGTCVNVGCVPKKI 288
Query: 89 MHYAGNMGSLFKMDSNPYGWSYNNLIHNWGKLVTTVQSHIRSLNFSYMTGLRSSKVKYMN 148
M A ++ + + +S YG+ N LV +IR LN Y L+ V+Y
Sbjct: 289 MFNAASIHDILE-NSRHYGFD-TQFSFNLPLLVERRDKYIRRLNDIYRQNLKKDNVEYFE 462
Query: 149 GLASLKDEHTV----------------------SYYLKGDMSKEELVTAKYILIATGCRP 186
G ASL E+ V + +++ K ILIA G +P
Sbjct: 463 GKASLLSENKVLIKKVKQQGHHYDDQEDDEEHYYEEDDEGDDEGQVIEGKNILIAVGNKP 642
Query: 187 SIPDDVEGAKELSITSDDIFSMKKDPGKTLIVGASYVALECAGFLNSLGYDVTVAVR-SI 245
P+ V+G E +I+SDD F +K + + IVG+ Y+A+E + LG + + R +
Sbjct: 643 IFPN-VKGI-ENTISSDDFFKIK-EAKRIGIVGSGYIAVELINVVKRLGMESYIFARGNR 813
Query: 246 VLRGFDSQCALKVKIYMEEQGVLFKHGVLPKKLSKMEGEKISVLFNDGTT-ELFDTVLYA 304
+LR FD +++ M++ + +++ K++ + +++ +DG E FD V+Y
Sbjct: 814 LLRKFDETIINELENDMKKNNINIITHANVEEIEKVKEKNLTIYLSDGRKYEHFDYVIYC 993
Query: 305 TGRKGDIDMLNLDQVKIQVNKNTNKIITNEVSCTNISNIFAVGDVAV--------DVP-- 354
GR + + LNL + I+ KN II ++ TN+ +I+AVGD + D+
Sbjct: 994 VGRSPNTEDLNLQALNIKTEKNY--IIVDDNQRTNVKHIYAVGDCCMVKKKQEIEDLNLL 1167
Query: 355 -----------------------ELAPVAIKAGEILARRLFNQSEEIMDYTFIPTSIYTP 391
+L PVAI AG +LA RLF + I +Y IP+ I++
Sbjct: 1168KLYNEEVYLKKKENTSGDQYYNVQLTPVAINAGRLLADRLFLKRSRITNYKLIPSVIFSH 1347
Query: 392 IEYGTCGYSEEKAYEIFGTSNVEVFLQEFNNLEISAVHREKHVRAQKDQYDTDVSSTCLS 451
GT GYSE++A +I+G NV+++ F NL S + AQK++ T L
Sbjct: 1348PPIGTIGYSEQEAIDIYGKENVKIYESRFTNLFFSVYDMDP---AQKEK-------TYL- 1494
Query: 452 KLVCLKNEDNRVVGFHYVGPNAGEITQGMALALRLKAKKSDFDKCIGIHPTDAESFMNL 510
KLVC+ E+ + G H VG NA EI QG A+AL++ A K DFD+ I IHPT AE F+ +
Sbjct: 1495KLVCVGKEE-LIKGLHIVGLNADEIVQGFAVALKMNATKKDFDETIPIHPTAAEEFVTM 1668
>Plasmodium_knowlesi_strain_H|PK4.chr05|PKH_051660|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) dihydrolipoamide
dehydrogenase, putative
Length = 1995
Score = 108 bits (269), Expect = 4e-24, Method: Composition-based stats.
Identities = 143/549 (26%), Positives = 225/549 (40%), Gaps = 75/549 (13%)
Frame = +1
Query: 32 YDYVVIGGGPGGMASAKEAASHGAKVLLFDYVKPSNKGTKWGIGGTCVNVGCVPKKLMHY 91
YD ++G G GG A+A A G KV++F G K IGGTCVNVGC+P K + Y
Sbjct: 340 YDVGILGCGVGGHAAAINAMERGLKVIIF-------TGDKDSIGGTCVNVGCIPSKSLLY 498
Query: 92 AGNMGSLFKMDSNPYGWS-YNNLIHNWGK---------LVTTVQSHIRSLNFSYMTGLRS 141
A K + Y + Y N GK L TVQ I L + +
Sbjct: 499 ATGKYRELKNLAKLYTYGIYTNAFRKSGKEDPVERNQLLADTVQIDIGKLKEYTQSVINK 678
Query: 142 SKVKYMNGLASLK----DEHTVSYYLKGDMSKEELVTA---------KYILIATGCRPSI 188
K NGL K EH Y +G + ++ ++ K I+IATG P+I
Sbjct: 679 LKGGIENGLKKKKFCKNSEHVQVIYERGHIVEKNIIKGEKSGKEYQVKNIIIATGSTPNI 858
Query: 189 PDDVEGAKELSITSDDIFSMKKDPGKTLIVGASYVALECAGFLNSLGYDVT--------- 239
PD++E ++ TSD ++ IVG + +E +LG +V
Sbjct: 859 PDNIEVDEKTVFTSDQAVKLEGLQKYMGIVGMGIIGIEFTDIYTALGSEVVSFDYSPQLL 1038
Query: 240 -------------VAVRSIVLRGFDSQCALKVKIYMEEQGVLFKHGVLPKKLSKMEGEKI 286
V ++S +R + C V+ Q V+ H + + E +K
Sbjct: 1039PLLDADVATYFERVFIKSKPMRVHLNTCIEYVRAGKGNQPVIIGH----SERNDAEEDKP 1206
Query: 287 SVLFNDGTTELFDTVLYATGRKGDIDMLNLDQVKIQVNK---NTNKIITNEVSCTNI-SN 342
N D+ L ATGRK + + + LD ++IQ+N+ + ++ + E + N
Sbjct: 1207IQRNNKIKETRVDSCLVATGRKPNTNNMGLDDLQIQMNRGFVSVDEHLRVERKDQGVYDN 1386
Query: 343 IFAVGDVAVDVPELAPVAIKAGEILARRLFNQSEEIMD------------------YTFI 384
IF +GD A LA A + + + +E + Y I
Sbjct: 1387IFCIGD-ANGKQMLAHTASHQALKVVDWIISNGKETHNNTVHSIASHNDWASKPIIYRNI 1563
Query: 385 PTSIYTPIEYGTCGYSEEKAYEIFGTSNV--EVFLQEFN------NLEISAVHREKHVRA 436
P+ YT E G +E++A +++ N+ E+ + N N +I+ R K+
Sbjct: 1564PSVCYTTPELAFVGLTEKEAKKLYPPENIGTEISFYKANSKVLCENNDITFPERSKNNSY 1743
Query: 437 QKDQYDTDVSSTCLSKLVCLKNEDNRVVGFHYVGPNAGEITQGMALALRLKAKKSDFDKC 496
K +Y+T +++ + K+V LK E ++G VG A + LAL LK D
Sbjct: 1744NKGKYNTVDNTSGMVKIVYLK-ESKEILGLFIVGSYASILIHEGVLALNLKLSVIDLAHM 1920
Query: 497 IGIHPTDAE 505
+ HPT +E
Sbjct: 1921VHSHPTISE 1947
>Plasmodium_knowlesi_strain_H|chr14|PKH_144930|Annotation|Plasmodium
_knowlesi_Sanger|(protein coding) lipoamide
dehydrogenase, putative
Length = 1542
Score = 97.4 bits (241), Expect = 7e-21, Method: Composition-based stats.
Identities = 143/518 (27%), Positives = 232/518 (44%), Gaps = 43/518 (8%)
Frame = +1
Query: 31 DYDYVVIGGGPGGMASAKEAASHGAKVLLFDYVKPSNKGTKWGIGGTCVNVGCVPKK-LM 89
DYD +VIGGGPGG + A + VL N +GGTC+N GC+P K L+
Sbjct: 73 DYDVIVIGGGPGGYVCSIRCAQNKLNVL--------NVNEDKKLGGTCLNRGCIPSKSLL 228
Query: 90 HYAGNMGSLFKMDSNPYGWSYNNLIHNWGKLVTTVQSHIRSLN-------------FSYM 136
H A N + N G +V VQ + +L+ +Y+
Sbjct: 229 HIAHNY------------YEAKNKFKQMGIIVDNVQLDVETLHKHKNKCMGNLADGITYL 372
Query: 137 TGLRSSKVKYMNGLASLKDEHTVSYYLKGDMSKEELVTAKYILIATGCRP-SIP------ 189
+ +KV ++ G S+ D ++V L KE+LVTA+ I+IATG +P IP
Sbjct: 373 --YKKNKVNHIIGHGSIVDGNSV---LVNSEGKEKLVTAERIVIATGSKPIEIPLKKLND 537
Query: 190 DDVEGAK----------ELSITSDDIFSMKKDPGKTLIVGASYVALECAGFLNSLGYDVT 239
D+V+ A+ ++ TSDDI + KK P K I+G + LE +G +V
Sbjct: 538 DNVKEAETVVDLLQYDHQMIQTSDDILNFKKIPKKISIIGGGVIGLEIGSVFAKMGSEVI 717
Query: 240 VAVRSIVLRGF-DSQCALKVKIYMEEQGVLFKHGVLPKKLSKMEGEKISVLFNDGTTELF 298
+ + L F D+ + ++ +E+ + F +E E+ ++ + T+
Sbjct: 718 IFEYNNRLCSFLDADVSRVLQKTLEKIKIKFAFNTSVIG-GNVENEQATIFAQNTKTKEI 894
Query: 299 -----DTVLYATGRKGDIDMLNLDQVKIQVNKNTNKIITNEVSCTNISNIFAVGDVAVDV 353
D VL GR+ + D LNLD+V I++ KN + + I A+GD A+D
Sbjct: 895 QKVTSDVVLVCVGRRPNFDNLNLDKVNIELGKNKKIQVDESFGVISHPTIKAIGD-AIDG 1071
Query: 354 PELAPVAIKAGEILARRLFNQ------SEEIMDYTFIPTSIYTPIEYGTCGYSEEKAYEI 407
P LA A + G ILA L ++ + ++Y +P+ IYT E + G +EEK ++
Sbjct: 1072PMLAHKAEEEGYILANILLSELKLNKPKKSHINYDLVPSVIYTHPEVASVGLNEEKCKQM 1251
Query: 408 FGTSNVEVFLQEFNNLEISAVHREKHVRAQKDQYDTDVSSTCLSKLVCLKNEDNRVVGFH 467
+ F +A R + + D +D L KL+ ++NE N+++G
Sbjct: 1252NLSFKAVTF-------PFAANSRSRTI----DDFDG------LIKLL-VENETNKILGSQ 1377
Query: 468 YVGPNAGEITQGMALALRLKAKKSDFDKCIGIHPTDAE 505
VG NA ++ +++ + K I HPT +E
Sbjct: 1378IVGNNASDLILPLSIYVGSGGSSKSLSKIIYAHPTFSE 1491
>Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_133040|Annotation|Plasmodi
um_knowlesi_Sanger|(protein coding) cyclic-nucleotide
binding protein
Length = 10143
Score = 28.9 bits (63), Expect = 3.5, Method: Composition-based stats.
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = -1
Query: 372 FNQSEEIMDYTFIPTSIYTPIEYGTCGYSEEKAYEIFGT 410
FN + + DY + + P Y C +SEE E+FGT
Sbjct: 7185 FNGNNSLFDYVKMVRVVVIP--YYDCSFSEEFKIEMFGT 7075
>Plasmodium_knowlesi_strain_H|PK4.chr09|PKH_093890|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) hypothetical
protein, conserved in Plasmodium species
Length = 1023
Score = 28.1 bits (61), Expect = 5.2, Method: Composition-based stats.
Identities = 19/56 (33%), Positives = 27/56 (48%)
Frame = +1
Query: 403 KAYEIFGTSNVEVFLQEFNNLEISAVHREKHVRAQKDQYDTDVSSTCLSKLVCLKN 458
K E+ E+FLQE + E VH E + QKD D + +K++ LKN
Sbjct: 391 KTKEVLLERRKELFLQELSGGEFVNVHAELFLDPQKDYIDAVKNK---NKMLTLKN 549
>Plasmodium_knowlesi_strain_H|PK4.chr03|PKH_031670|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) tRNA
guanylyltransferase, putative
Length = 849
Score = 28.1 bits (61), Expect = 5.7, Method: Composition-based stats.
Identities = 34/125 (27%), Positives = 51/125 (40%), Gaps = 9/125 (7%)
Frame = +1
Query: 296 ELFDTVLYATGRKGDIDMLNLDQVKIQVNKNTNKIITNEVSCTNISNIFAVGDVAVDVPE 355
E FD + A G + L + K+ N+ +KI+TN VSC + S F + + E
Sbjct: 187 EKFDEIDLAYGHSDEYSFLFRKKTKVW-NRRYDKILTNVVSCFSGSFPF-LWKIFFPEQE 360
Query: 356 LAPVAIKAGEILARRLFNQSEEIMDY-TFIPTSIYTPIEYGTC--------GYSEEKAYE 406
L V G I+ L E DY + + +Y C GYS ++AY
Sbjct: 361 LLYVPSFDGRIV---LLPTEREAKDYFRWRQVDCHINTQYNECFWNLINKDGYSHQQAYN 531
Query: 407 IFGTS 411
T+
Sbjct: 532 TLITT 546
>Plasmodium_knowlesi_strain_H|PK4.chr05|PKH_052140|Annotation|Plasmodi
um_knowlesi_Sanger|(protein coding) hypothetical protein,
conserved in Plasmodium species
Length = 16035
Score = 27.3 bits (59), Expect = 8.3, Method: Composition-based stats.
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = +3
Query: 263 EEQGVLFKHGVLPKKLSKMEGEKISVLF 290
E GV F HG + +L GE++ +LF
Sbjct: 1440 ENAGVRFSHGTIQSELCIQRGERLPLLF 1523
>Plasmodium_knowlesi_strain_H|PK4.chr08|PKH_082970|Annotation|Plasmodi
um_knowlesi_Sanger|(protein coding) helicase, putative
Length = 6180
Score = 27.3 bits (59), Expect = 9.9, Method: Composition-based stats.
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +1
Query: 318 QVKIQVNKNTNKIITNEVSCTNISNIFAVGDVAVDVP 354
Q + V+ N I N CTNI N+F+ G +P
Sbjct: 4195 QAIVPVHVNQKDEINNGSLCTNIINMFSSGRTCFSIP 4305
Database: cds.fasta
Posted date: Mar 6, 2008 6:01 PM
Number of letters in database: 11,140,562
Number of sequences in database: 5106
Lambda K H
0.316 0.134 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5106
Number of Hits to DB: 6,449,690
Number of extensions: 93766
Number of successful extensions: 547
Number of sequences better than 10.0: 14
Number of HSP's gapped: 534
Number of HSP's successfully gapped: 15
Length of query: 529
Length of database: 3,713,520
Length adjustment: 97
Effective length of query: 432
Effective length of database: 3,218,238
Effective search space: 1390278816
Effective search space used: 1390278816
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 41 (20.4 bits)
