TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= sel3_Pknowlesi_prot
(344 letters)
Database: cds.fa
5106 sequences; 11,140,562 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|chr14|PKH_142340|Annotation|Plasmod... 566 e-162
Plasmodium_knowlesi_strain_H|PK4.chr09|PKH_093850|Annotation|Pla... 30 0.70
Plasmodium_knowlesi_strain_H|PK4.chr05|PKH_050680|Annotation|Pla... 29 2.0
Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_134460|Annotation|Pla... 28 4.7
Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_121780|Annotat... 27 7.9
Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_120090|Annotat... 27 8.0
Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_132690|Annotation|Pla... 27 8.3
Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_072690|Annotation|Pla... 27 8.3
Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_124940|Annotat... 27 8.4
>Plasmodium_knowlesi_strain_H|chr14|PKH_142340|Annotation|Plasmodium
_knowlesi_Sanger|(protein coding) hypothetical protein,
conserved in Plasmodium species
Length = 972
Score = 566 bits (1458), Expect = e-162, Method: Composition-based stats.
Identities = 323/344 (93%), Positives = 323/344 (93%)
Frame = +1
Query: 1 MVLNKVYLLTILVLFYVNTLCVEAG*SKKLHIKLPNEDDDYLGKLINISSKITKYAQNNK 60
MVLNKVYLLTIL LPNEDDDYLGKLINISSKITKYAQNNK
Sbjct: 1 MVLNKVYLLTIL---------------------LPNEDDDYLGKLINISSKITKYAQNNK 117
Query: 61 FKIAKILSTSALSAYSLNWVYQTGVTLLKDPHYSLFVPSNNYMNNAIRRIRTNYPVKSYT 120
FKIAKILSTSALSAYSLNWVYQTGVTLLKDPHYSLFVPSNNYMNNAIRRIRTNYPVKSYT
Sbjct: 118 FKIAKILSTSALSAYSLNWVYQTGVTLLKDPHYSLFVPSNNYMNNAIRRIRTNYPVKSYT 297
Query: 121 FKVNKILERNFHHEYANTEMGQIYVVNNFVNFLNFLPYKWRKKCTYNFCKYKEFENIGNL 180
FKVNKILERNFHHEYANTEMGQIYVVNNFVNFLNFLPYKWRKKCTYNFCKYKEFENIGNL
Sbjct: 298 FKVNKILERNFHHEYANTEMGQIYVVNNFVNFLNFLPYKWRKKCTYNFCKYKEFENIGNL 477
Query: 181 FNCIRISKHEGPILLFQGKLKKQYWIHLPLKYEIVKNGEEDSLCTLTFTPLHKYYSDYTI 240
FNCIRISKHEGPILLFQGKLKKQYWIHLPLKYEIVKNGEEDSLCTLTFTPLHKYYSDYTI
Sbjct: 478 FNCIRISKHEGPILLFQGKLKKQYWIHLPLKYEIVKNGEEDSLCTLTFTPLHKYYSDYTI 657
Query: 241 EIKLVKEKENNNVTFITSVKCANKNNGNGNSFYINVIQNIAMFLAYDIFEGINNNIHVVH 300
EIKLVKEKENNNVTFITSVKCANKNNGNGNSFYINVIQNIAMFLAYDIFEGINNNIHVVH
Sbjct: 658 EIKLVKEKENNNVTFITSVKCANKNNGNGNSFYINVIQNIAMFLAYDIFEGINNNIHVVH 837
Query: 301 RRNANYRKTTFNTSNVTLKRKKKTNFQFVLSPVINPWSFKIRRS 344
RRNANYRKTTFNTSNVTLKRKKKTNFQFVLSPVINPWSFKIRRS
Sbjct: 838 RRNANYRKTTFNTSNVTLKRKKKTNFQFVLSPVINPWSFKIRRS 969
>Plasmodium_knowlesi_strain_H|PK4.chr09|PKH_093850|Annotation|Plasmodi
um_knowlesi_Sanger|(protein coding) hypothetical protein,
conserved in Plasmodium species
Length = 7470
Score = 30.4 bits (67), Expect = 0.70, Method: Composition-based stats.
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +1
Query: 159 KWRKKCTYNFCKYKEFENIGN-LFNCIRISKHEG 191
+WRK+ + +FC + E +NI L+N SK EG
Sbjct: 3646 RWRKESSSDFCNFHEMKNIERVLYNSRVASKGEG 3747
>Plasmodium_knowlesi_strain_H|PK4.chr05|PKH_050680|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) hypothetical
protein, conserved in Plasmodium species
Length = 3138
Score = 28.9 bits (63), Expect = 2.0, Method: Composition-based stats.
Identities = 15/42 (35%), Positives = 25/42 (59%)
Frame = +1
Query: 212 YEIVKNGEEDSLCTLTFTPLHKYYSDYTIEIKLVKEKENNNV 253
+E+ KN +++C L +T +YS Y E+ VKE+ NN+
Sbjct: 415 HEMSKN---ENICMLKYTSYESFYSKYDNEMDKVKEQGENNI 531
>Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_134460|Annotation|Plasmodi
um_knowlesi_Sanger|(protein coding) hypothetical protein,
conserved in Plasmodium species
Length = 1665
Score = 27.7 bits (60), Expect = 4.7, Method: Composition-based stats.
Identities = 15/35 (42%), Positives = 18/35 (51%)
Frame = +3
Query: 159 KWRKKCTYNFCKYKEFENIGNLFNCIRISKHEGPI 193
KWRKKC N + +E+ G N R SK E I
Sbjct: 1023 KWRKKCKRNEGSRR*YESRGQQCNGGRKSKGEASI 1127
>Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_121780|Annotation|P
lasmodium_knowlesi_Sanger|(protein coding) hypothetical
protein, conserved in Plasmodium species
Length = 1926
Score = 26.9 bits (58), Expect = 7.9, Method: Composition-based stats.
Identities = 17/41 (41%), Positives = 24/41 (58%)
Frame = +1
Query: 133 HEYANTEMGQIYVVNNFVNFLNFLPYKWRKKCTYNFCKYKE 173
H+ TE +IYV+ N +NF N L K+R Y +C+ KE
Sbjct: 1495 HDGKETE-NEIYVLKNKINFNNDLKKKYR---FYFYCRMKE 1605
>Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_120090|Annotation
|Plasmodium_knowlesi_Sanger|(protein coding)
hypothetical protein, conserved in Plasmodium species
Length = 1842
Score = 26.9 bits (58), Expect = 8.0, Method: Composition-based stats.
Identities = 11/35 (31%), Positives = 24/35 (68%)
Frame = +1
Query: 221 DSLCTLTFTPLHKYYSDYTIEIKLVKEKENNNVTF 255
D + T+TF L+K Y+D+ ++K ++ +EN+ + +
Sbjct: 124 DIILTITFFALYKLYNDFK-KMKFLQPRENHQIIY 225
>Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_132690|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) hypothetical
protein, conserved in Plasmodium species
Length = 918
Score = 26.6 bits (57), Expect = 8.3, Method: Composition-based stats.
Identities = 14/32 (43%), Positives = 17/32 (53%), Gaps = 1/32 (3%)
Frame = +1
Query: 151 NFLNFLPYKWRKK-CTYNFCKYKEFENIGNLF 181
NF FL Y ++KK C NFCK + N F
Sbjct: 517 NFYTFLSYDYKKKSCIQNFCKKYSQVEVSNQF 612
>Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_072690|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) SICAvar antigen
(fragment)
Length = 970
Score = 26.6 bits (57), Expect = 8.3, Method: Composition-based stats.
Identities = 18/74 (24%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
Frame = +1
Query: 251 NNVTFITSVKCANKNNGNGNSFYI-NVIQNIAMFLAYDIFEGINNNIHVVHRRNANYRKT 309
N + + +K N N+G N+F + +V+ N + L + +GI + V N +
Sbjct: 742 NKTSVVEFMKLDNSNSGQSNTFSLADVLMNSEIQLPENTIQGILKEM-VADSANGTVEPS 918
Query: 310 TFNTSNVTLKRKKK 323
T+ TL+++ K
Sbjct: 919 KMKTAVQTLEKESK 960
>Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_124940|Annotation
|Plasmodium_knowlesi_Sanger|(protein coding)
hypothetical protein, conserved in Plasmodium species
Length = 2133
Score = 26.6 bits (57), Expect = 8.4, Method: Composition-based stats.
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = -1
Query: 141 GQIYVVNNFVNFLNFLPYKWRKKCTYNFCKY 171
G IY N+F FL+F +K YN C Y
Sbjct: 327 GSIYQANSFYFFLHFCAFKQITDILYNVCAY 235
Database: cds.fa
Posted date: Mar 10, 2008 1:57 PM
Number of letters in database: 11,140,562
Number of sequences in database: 5106
Lambda K H
0.323 0.138 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5106
Number of Hits to DB: 5,393,821
Number of extensions: 104004
Number of successful extensions: 805
Number of sequences better than 10.0: 55
Number of HSP's gapped: 803
Number of HSP's successfully gapped: 57
Length of query: 344
Length of database: 3,713,520
Length adjustment: 93
Effective length of query: 251
Effective length of database: 3,238,662
Effective search space: 812904162
Effective search space used: 812904162
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 40 (20.0 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: sel3_Pknowlesi_prot
# Database: cds.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|chr14|PKH_142340|Annotation|Plasmodium_knowlesi_Sanger|(protein 93.90 344 21 1 1 344 1 969 4e-162 566
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr09|PKH_093850|Annotation|Plasmodium_knowlesi_Sanger|(protein 41.18 34 19 1 159 191 3646 3747 0.70 30.4
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr05|PKH_050680|Annotation|Plasmodium_knowlesi_Sanger|(protein 35.71 42 27 1 212 253 415 531 2.0 28.9
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_134460|Annotation|Plasmodium_knowlesi_Sanger|(protein 42.86 35 20 0 159 193 1023 1127 4.7 27.7
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_121780|Annotation|Plasmodium_knowlesi_Sanger|(protein 41.46 41 24 2 133 173 1495 1605 7.9 26.9
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_120090|Annotation|Plasmodium_knowlesi_Sanger|(protein 31.43 35 24 1 221 255 124 225 8.0 26.9
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_132690|Annotation|Plasmodium_knowlesi_Sanger|(protein 43.75 32 17 1 151 181 517 612 8.3 26.6
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_072690|Annotation|Plasmodium_knowlesi_Sanger|(protein 24.32 74 55 2 251 323 742 960 8.3 26.6
sel3_Pknowlesi_prot Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|PKH_124940|Annotation|Plasmodium_knowlesi_Sanger|(protein 41.94 31 18 0 141 171 327 235 8.4 26.6
