TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= MAL8P186_Plasmodiumfalciparum_protein
(351 letters)
Database: PknowlesiGenomic_plasmoDB-5.4.fasta
14 sequences; 23,462,190 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_... 183 1e-46
Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds... 30 2.6
>Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 3159096
Score = 183 bits (465), Expect = 1e-46, Method: Composition-based stats.
Identities = 125/352 (35%), Positives = 180/352 (51%), Gaps = 1/352 (0%)
Frame = +2
Query: 1 MILKKVYXXXXXXXXXXXXRTVDSUXSKQLHIKLPDEDDDYLAKLIGVFNDICTYSINNK 60
M+L KVY V++ SK+LHIKLP+EDDDYL KLI + + I Y+ NNK
Sbjct: 1037351 MVLNKVYLLTILVLFYVNTLCVEAG*SKKLHIKLPNEDDDYLGKLINISSKITKYAQNNK 1037530
Query: 61 EKIAKILSTSAVSVYTITSLYNSGITFKKNPHYSFFLPSQKYXXXXXXXXXXXXXXXXXX 120
KIAKILSTSA+S Y++ +Y +G+T K+PHYS F+PS Y
Sbjct: 1037531 FKIAKILSTSALSAYSLNWVYQTGVTLLKDPHYSLFVPSNNYMNNAIRRIRTNYPVKSYT 1037710
Query: 121 XXXXXXXPIFERTFTNKH-NIQMSKTYXXXXXXXXXXXXPYKLKKESLYNFSKYKEFDGL 179
I ER F +++ N +M + Y PYK +K+ YNF KYKEF+
Sbjct: 1037711 FKVNK---ILERNFHHEYANTEMGQIYVVNNFVNFLNFLPYKWRKKCTYNFCKYKEFEN- 1037878
Query: 180 KYVENPFTQVQVLNHEDNIIVYQAKTKKHYWIHLPFKYNIIKISDDNTTSYILMFIPLYK 239
+ N F +++ HE I+++Q K KK YWIHLP KY I+K ++++ L F PL+K
Sbjct: 1037879 --IGNLFNCIRISKHEGPILLFQGKLKKQYWIHLPLKYEIVKNGEEDSLC-TLTFTPLHK 1038049
Query: 240 YYSNYIIQMKITSNNNNQNVTFSSCIKQEKTEHIQNNSFHYDIIKNISKHITYXXXXXXX 299
YYS+Y I++K+ N NVTF + +K + NSF+ ++I+NI+ + Y
Sbjct: 1038050 YYSDYTIEIKLVKEKENNNVTFITSVKCANKNNGNGNSFYINVIQNIAMFLAYDIFEGIN 1038229
Query: 300 XXXXXLYMRNVKPGKYNFINTTNALQXXXXXXXXXXFTLPSFKSLPFKFKRT 351
++ RN K F NT+N P FK +R+
Sbjct: 1038230 NNIHVVHRRNANYRKTTF-NTSNVTLKRKKKTNFQFVLSPVINPWSFKIRRS 1038382
>Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2372884
Score = 29.6 bits (65), Expect = 2.6, Method: Composition-based stats.
Identities = 11/24 (45%), Positives = 19/24 (79%)
Frame = +2
Query: 249 KITSNNNNQNVTFSSCIKQEKTEH 272
K+++N +N+NVT SC ++EK +H
Sbjct: 527711 KVSTNGSNKNVT*ESCTRREKKQH 527782
Database: PknowlesiGenomic_plasmoDB-5.4.fasta
Posted date: Feb 25, 2008 11:43 AM
Number of letters in database: 23,462,190
Number of sequences in database: 14
Lambda K H
0.322 0.136 0.402
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 8,267,142
Number of extensions: 125044
Number of successful extensions: 884
Number of sequences better than 10.0: 11
Number of HSP's gapped: 881
Number of HSP's successfully gapped: 17
Length of query: 351
Length of database: 7,820,730
Length adjustment: 99
Effective length of query: 252
Effective length of database: 7,819,344
Effective search space: 1970474688
Effective search space used: 1970474688
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 60 (27.7 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: MAL8P186_Plasmodiumfalciparum_protein
# Database: PknowlesiGenomic_plasmoDB-5.4.fasta
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
MAL8P186_Plasmodiumfalciparum_protein Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 42.05 352 203 5 1 351 1037351 1038382 1e-46 183
MAL8P186_Plasmodiumfalciparum_protein Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 45.83 24 13 0 249 272 527711 527782 2.6 29.6
