TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= Sel2_prot
(231 letters)
Database: cds.fasta
5106 sequences; 11,140,562 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_072950|Annotation|Pla... 287 2e-78
Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|PKH_111780|An... 28 1.5
Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|PKH_114380|An... 28 1.5
Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_070150|Annotation|Pla... 27 3.5
Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|PKH_111110|An... 26 7.3
>Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_072950|Annotation|Plasmo
dium_knowlesi_Sanger|(protein coding) hypothetical
protein, conserved in Plasmodium species
Length = 564
Score = 287 bits (734), Expect = 2e-78, Method: Composition-based stats.
Identities = 187/231 (80%), Positives = 187/231 (80%)
Frame = +1
Query: 1 MYYLKLFLKLLLLLFLSYDFIVLKNGTREVVHGDRNFLKESYEFLFVQKDTFPSYINRDN 60
MYYLKLFLKLLLLLFLSYDFIVLKNGTREVVHGDRNFLKESYEFLF
Sbjct: 1 MYYLKLFLKLLLLLFLSYDFIVLKNGTREVVHGDRNFLKESYEFLF-------------- 138
Query: 61 QISIYFCKS*QSHYVLSRIKKYFDLLNHGEDTEIYFEEDSYQADNSSNGEEKYVIFLRNI 120
DTEIYFEEDSYQADNSSNGEEKYVIFLRNI
Sbjct: 139 ------------------------------DTEIYFEEDSYQADNSSNGEEKYVIFLRNI 228
Query: 121 KAALIYRLICRFVLFIYVLIAASLIFPHYLKPYIPPILLNNRRFNEVFENMQQKKLMVLG 180
KAALIYRLICRFVLFIYVLIAASLIFPHYLKPYIPPILLNNRRFNEVFENMQQKKLMVLG
Sbjct: 229 KAALIYRLICRFVLFIYVLIAASLIFPHYLKPYIPPILLNNRRFNEVFENMQQKKLMVLG 408
Query: 181 IMFFSYNVIYGMLCNTSVIHVYQNRNLIYDDYYSDHLFVQRLRENMTHVPG 231
IMFFSYNVIYGMLCNTSVIHVYQNRNLIYDDYYSDHLFVQRLRENMTHVPG
Sbjct: 409 IMFFSYNVIYGMLCNTSVIHVYQNRNLIYDDYYSDHLFVQRLRENMTHVPG 561
>Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|PKH_111780|Annotat
ion|Plasmodium_knowlesi_Sanger|(protein coding)
hypothetical protein, conserved in Plasmodium species
Length = 5463
Score = 28.5 bits (62), Expect = 1.5, Method: Composition-based stats.
Identities = 20/72 (27%), Positives = 38/72 (52%), Gaps = 3/72 (4%)
Frame = +1
Query: 64 IYFCKS*QSHYVLSRIKKYFDLLNHGEDTEIYFEEDSYQADNSSNGEEKYVI---FLRNI 120
IY +S + + +K YF+ + +IYFEE+++Q N +EK++ F+ N+
Sbjct: 4798 IYGGRSPRDESFENAMKAYFNAKAIYDKWKIYFEENNFQG----NAQEKFLFNLSFIYNV 4965
Query: 121 KAALIYRLICRF 132
L++ IC +
Sbjct: 4966 YNYLVWIYICTY 5001
>Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|PKH_114380|Annot
ation|Plasmodium_knowlesi_Sanger|(protein coding)
SICAvar antigen (fragment)
Length = 912
Score = 28.5 bits (62), Expect = 1.5, Method: Composition-based stats.
Identities = 18/39 (46%), Positives = 22/39 (56%), Gaps = 2/39 (5%)
Frame = -3
Query: 122 AALIYRLICRF--VLFIYVLIAASLIFPHYLKPYIPPIL 158
A IYR ICR VL+IY ++ S PY+PPIL
Sbjct: 589 AEWIYRTICRCL*VLYIYPILHPS--------PYVPPIL 497
>Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_070150|Annotation|Plasmodi
um_knowlesi_Sanger|(protein coding) hypothetical protein,
conserved in Plasmodium species
Length = 6954
Score = 26.9 bits (58), Expect = 3.5, Method: Composition-based stats.
Identities = 11/41 (26%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -2
Query: 125 IYRLICRFVLFIYVLIAASLIFPHYLKP--YIPPILLNNRR 163
++RL+C F++ + A +FP P +PP+ +R
Sbjct: 5342 LFRLVCNSSPFLWYMWALGGLFPRLFPPPGKVPPVYFETKR 5220
>Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|PKH_111110|Annotat
ion|Plasmodium_knowlesi_Sanger|(protein coding)
hypothetical protein, conserved in Plasmodium species
Length = 9525
Score = 26.2 bits (56), Expect = 7.3, Method: Composition-based stats.
Identities = 18/44 (40%), Positives = 25/44 (56%), Gaps = 4/44 (9%)
Frame = +1
Query: 192 MLCNTSVIHVYQNRNLIYDDYYSDHL--FVQRL--RENMTHVPG 231
+LC S +QN + +YDD Y DHL ++ R RE + VPG
Sbjct: 2863 LLCRNSKNTCFQNVSKVYDDIY-DHLEKYILRYKSREGLV-VPG 2988
Database: cds.fasta
Posted date: Mar 6, 2008 6:01 PM
Number of letters in database: 11,140,562
Number of sequences in database: 5106
Lambda K H
0.332 0.147 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5106
Number of Hits to DB: 3,734,200
Number of extensions: 71623
Number of successful extensions: 811
Number of sequences better than 10.0: 37
Number of HSP's gapped: 809
Number of HSP's successfully gapped: 39
Length of query: 231
Length of database: 3,713,520
Length adjustment: 89
Effective length of query: 142
Effective length of database: 3,259,086
Effective search space: 462790212
Effective search space used: 462790212
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 38 (19.2 bits)
