# TBLASTN 2.2.17 [Aug-26-2007]
# Query:
# Database: genoma_PlasmoDB.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
1_0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 50.87 230 109 1 2 227 1322389 1323078 2e-55 212
1_0 Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 30.12 83 58 2 76 158 2271384 2271142 0.35 31.6
1_0 Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 34.62 52 27 3 184 228 1227980 1228132 0.51 31.2
1_0 Plasmodium_knowlesi_strain_H|chr04|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 34.15 41 26 1 112 151 577455 577577 0.85 30.4
1_0 Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 20.97 62 49 0 132 193 2621022 2621207 2.2 28.9
1_0 Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 33.96 53 34 1 164 215 840011 839853 3.9 28.1
1_0 Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 55.00 20 9 0 168 187 175052 174993 2.6 28.9
1_0 Plasmodium_knowlesi_strain_H|PK4.chr06|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 32.50 40 27 1 120 159 749652 749545 8.8 26.9
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query=
(229 letters)
Database: genoma_PlasmoDB.fa
14 sequences; 23,462,190 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmod... 212 2e-55
Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_... 32 0.35
Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmod... 31 0.51
Plasmodium_knowlesi_strain_H|chr04|2007-02-22|ds-DNA|Plasmodium_... 30 0.85
Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|... 29 2.2
Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-DNA|Plasmodium_... 29 2.6
Plasmodium_knowlesi_strain_H|PK4.chr06|2007-02-22|ds-DNA|Plasmod... 27 8.8
>Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1496036
Score = 212 bits (539), Expect = 2e-55, Method: Composition-based stats.
Identities = 117/230 (50%), Positives = 169/230 (73%), Gaps = 4/230 (1%)
Frame = +1
Query: 2 KAYYVVIILKVVLLLFFSYDFIFLKNEDNIIKGKGSSFLKSSYELLFVQKDVLLPHIKKN 61
K YY+ + LK++LLLF SYDFI LKN + +FLK SYE LFVQKD +I ++
Sbjct: 1322389 KMYYLKLFLKLLLLLFLSYDFIVLKNGTREVVHGDRNFLKESYEFLFVQKDTFPSYINRD 1322568
Query: 62 NQISIFLCRS*QSQYIINKIKNYFDILNKGRETEIYFENNNYIVDN----DQKYILFLMS 117
NQISI+ C+S*QS Y++++IK YFD+LN G +TEIYFE ++Y DN ++KY++FL +
Sbjct: 1322569 NQISIYFCKS*QSHYVLSRIKKYFDLLNHGEDTEIYFEEDSYQADNSSNGEEKYVIFLRN 1322748
Query: 118 IQPPLFFTLICYFILFIFILIALNIYLPKHCEFFIPSFLLNNTKFIETFEKMRKKKIIVS 177
I+ L + LIC F+LFI++LIA ++ P + + +IP LLNN +F E FE M++KK++V
Sbjct: 1322749 IKAALIYRLICRFVLFIYVLIAASLIFPHYLKPYIPPILLNNRRFNEVFENMQQKKLMVL 1322928
Query: 178 SIMFLAYNIIYSILCNTNEIHVYQNKNLIYKDYFNEYFFIQTLRTNLKNI 227
IMF +YN+IY +LCNT+ IHVYQN+NLIY DY++++ F+Q LR N+ ++
Sbjct: 1322929 GIMFFSYNVIYGMLCNTSVIHVYQNRNLIYDDYYSDHLFVQRLRENMTHV 1323078
>Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 3159096
Score = 31.6 bits (70), Expect = 0.35, Method: Composition-based stats.
Identities = 25/83 (30%), Positives = 44/83 (53%)
Frame = -1
Query: 76 YIINKIKNYFDILNKGRETEIYFENNNYIVDNDQKYILFLMSIQPPLFFTLICYFILFIF 135
Y I+++KN + +N I +ENNNY +DN +Y +FL++ + C +LF+F
Sbjct: 2271384 YHIDQLKNVNESINAELMKYIQYENNNYTIDN-YRYDVFLITSGFINQLNIFCD-LLFLF 2271211
Query: 136 ILIALNIYLPKHCEFFIPSFLLN 158
+ +I L C F +L++
Sbjct: 2271210 HVYDYSIKLFSVCLFIYAYYLVH 2271142
>Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2147124
Score = 31.2 bits (69), Expect = 0.51, Method: Composition-based stats.
Identities = 18/52 (34%), Positives = 30/52 (57%), Gaps = 7/52 (13%)
Frame = +2
Query: 184 YNIIYSILCNTNEIHVYQNKNLIYKDYFNE------YFFIQTL-RTNLKNIH 228
Y +IY + C + I +NKN YK+Y N YFF++ L + +L+N++
Sbjct: 1227980 YTVIY-LCCKMSIIKRLKNKNFHYKNYLNSSGEKSIYFFLENLDKHDLQNVN 1228132
>Plasmodium_knowlesi_strain_H|chr04|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 785142
Score = 30.4 bits (67), Expect = 0.85, Method: Composition-based stats.
Identities = 14/41 (34%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +3
Query: 112 ILFLMSIQPPLFFTL-ICYFILFIFILIALNIYLPKHCEFF 151
I+F+ PP + ++ + YF+ + +LI LN+YL C F
Sbjct: 577455 IVFVHGFAPPPYLSMKVAYFLSALDLLIILNLYLGGTCSDF 577577
>Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 3128370
Score = 28.9 bits (63), Expect = 2.2, Method: Composition-based stats.
Identities = 13/62 (20%), Positives = 32/62 (51%)
Frame = +3
Query: 132 LFIFILIALNIYLPKHCEFFIPSFLLNNTKFIETFEKMRKKKIIVSSIMFLAYNIIYSIL 191
++I + + LNI++ HC ++ +L + + F + +++S ++ L IY +
Sbjct: 2621022 VYILLRVRLNIHVNMHCSYWEKEEILIRNRGMRNFGSVPANCLVISGVILLTRTCIYIHI 2621201
Query: 192 CN 193
C+
Sbjct: 2621202 CS 2621207
Score = 28.1 bits (61), Expect = 3.9, Method: Composition-based stats.
Identities = 18/53 (33%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = -2
Query: 164 ETFEKMRKKKIIVSSIMFLAYNIIYS-ILCNTNEIHVYQNKNLIYKDYFNEYF 215
E +K+RK KI++ + ++ I + ILC EI +Y N L D N +F
Sbjct: 840011 EEEKKIRKSKILIVGLNGVSSEICKNLILCGVKEIGIYDNDILRMDDLDNLFF 839853
>Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 726886
Score = 28.9 bits (63), Expect = 2.6, Method: Composition-based stats.
Identities = 11/20 (55%), Positives = 17/20 (85%)
Frame = -3
Query: 168 KMRKKKIIVSSIMFLAYNII 187
K +K K+I++S+MFL YNI+
Sbjct: 175052 KSQKGKLIIASVMFLLYNIL 174993
>Plasmodium_knowlesi_strain_H|PK4.chr06|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1053092
Score = 26.9 bits (58), Expect = 8.8, Method: Composition-based stats.
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = -3
Query: 120 PPLFFTLICYFILFIFILIALNIYLPKHCEFFIPSFLLNN 159
PP + +C I F+ + N+Y+P F P FLL +
Sbjct: 749652 PPFEYIPLCTLIFFL----STNLYVPSFTPFLHPPFLLRS 749545
Database: genoma_PlasmoDB.fa
Posted date: Mar 10, 2008 2:39 PM
Number of letters in database: 23,462,190
Number of sequences in database: 14
Lambda K H
0.334 0.149 0.440
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 9,824,607
Number of extensions: 253790
Number of successful extensions: 6304
Number of sequences better than 10.0: 14
Number of HSP's gapped: 6277
Number of HSP's successfully gapped: 343
Length of query: 229
Length of database: 7,820,730
Length adjustment: 95
Effective length of query: 134
Effective length of database: 7,819,400
Effective search space: 1047799600
Effective search space used: 1047799600
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 58 (26.9 bits)
