# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SP1_P.falciparum
# Database: genoma_PlasmoDB.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SP1_P.falciparum Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 55.00 60 26 1 1 59 2085057 2084878 1e-11 64.7
SP1_P.falciparum Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 70.00 30 9 0 87 116 2084430 2084341 2e-04 40.0
SP1_P.falciparum Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 40.00 30 15 1 41 67 2162164 2162253 0.30 29.6
SP1_P.falciparum Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 30.00 40 26 1 31 68 2015509 2015628 7.5 25.0
SP1_P.falciparum Plasmodium_knowlesi_strain_H|PK4.chr02|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 32.61 46 28 1 17 59 310530 310393 8.4 25.0
TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= SP1_P.falciparum
(116 letters)
Database: genoma_PlasmoDB.fa
14 sequences; 23,462,190 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmod... 65 1e-11
Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmod... 25 7.5
Plasmodium_knowlesi_strain_H|PK4.chr02|2007-02-22|ds-DNA|Plasmod... 25 8.4
>Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2200295
Score = 64.7 bits (156), Expect = 1e-11, Method: Composition-based stats.
Identities = 33/60 (55%), Positives = 46/60 (76%), Gaps = 1/60 (1%)
Frame = -3
Query: 1 MDDRKENKKN-ADYEIFLRDRKLKYEKIRKRNALHNKFILPIINFIKGIINFIKTVINIL 59
M+ +KEN++N +YE LRDRKLKYE +RK++ LHN+F+ PI N I INF+KT+ NI+
Sbjct: 2085057 MNRKKENQENEGEYENVLRDRKLKYENLRKKSTLHNRFMSPIFNLINSAINFLKTLFNIV 2084878
Score = 40.0 bits (92), Expect = 2e-04, Method: Composition-based stats.
Identities = 21/30 (70%), Positives = 25/30 (83%)
Frame = -3
Query: 87 FFKKKRTGGLPKSRIMELKNLGTCLGSTUG 116
F KK++ G L KSR+MELKNLGTCLGS+ G
Sbjct: 2084430 FNKKRKMGTLQKSRMMELKNLGTCLGSS*G 2084341
Score = 29.6 bits (65), Expect = 0.30, Method: Composition-based stats.
Identities = 12/30 (40%), Positives = 22/30 (73%), Gaps = 3/30 (10%)
Frame = +1
Query: 41 IINFIKGIINFIKTVI---NILAIFFKTLL 67
I++F+K I+NF++ +I +L IFF+ L+
Sbjct: 2162164 IVSFVKIIVNFVRIIIVFFRVLIIFFRILI 2162253
>Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2147124
Score = 25.0 bits (53), Expect = 7.5, Method: Composition-based stats.
Identities = 12/40 (30%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = +1
Query: 31 NALHNKFILPIINFIKGIINFIKTVINILAIF--FKTLLG 68
N LHN I+P++NF+ + ++ I F F+ +G
Sbjct: 2015509 NILHNAVIIPLMNFLSHPFRYTMELVLIAFAFRPFRPCIG 2015628
>Plasmodium_knowlesi_strain_H|PK4.chr02|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1770351
Score = 25.0 bits (53), Expect = 8.4, Method: Composition-based stats.
Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 3/46 (6%)
Frame = -1
Query: 17 LRDRKLKYEKIRKRNALHNKF---ILPIINFIKGIINFIKTVINIL 59
+RD +L EKI+ L N I+P++ F+ GI++ +K + I+
Sbjct: 310530 IRDHELVKEKIKHVAELSNLIDLKIIPLLMFLNGIVDKLKGYLLII 310393
Database: genoma_PlasmoDB.fa
Posted date: Mar 10, 2008 2:39 PM
Number of letters in database: 23,462,190
Number of sequences in database: 14
Lambda K H
0.322 0.140 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 3,359,089
Number of extensions: 53668
Number of successful extensions: 567
Number of sequences better than 10.0: 11
Number of HSP's gapped: 566
Number of HSP's successfully gapped: 48
Length of query: 116
Length of database: 7,820,730
Length adjustment: 84
Effective length of query: 32
Effective length of database: 7,819,554
Effective search space: 250225728
Effective search space used: 250225728
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 52 (24.6 bits)
