TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2)
# Homo sapiens # Complete
(448 letters)
Database: genoma.fa
14 sequences; 23,462,190 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmod... 49 5e-06
>Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1496036
Score = 48.9 bits (115), Expect = 5e-06, Method: Composition-based stats.
Identities = 31/102 (30%), Positives = 47/102 (46%)
Frame = -3
Query: 241 SAVVGDVLVLTKPLGTQVAVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMFNMATLNR 300
S GD+++ TK G + AH RW + + +E+ L+ + +
Sbjct: 409614 SVKAGDIIITTKMFGFGFIMAAHICKKAKARW----IYICLDEMLLSNR----------K 409477
Query: 301 TAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIH 342
+ L+ NA A TD+TGFGILGH + K R E+ F H
Sbjct: 409476 SGLYLLQNNNAKACTDVTGFGILGHLNEMIKCSRREIYFASH 409351
Score = 44.7 bits (104), Expect = 1e-04, Method: Composition-based stats.
Identities = 25/84 (29%), Positives = 49/84 (58%)
Frame = -3
Query: 132 SLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLLSVSQSMSEEEREKV 191
+LVQT DFF ++D Y++G I + LSD+Y+MG T + +L+ V ++ ++ ++++
Sbjct: 410157 ALVQTIDFFKSFIDDEYILGEIIAIHCLSDVYSMGGTGICALCVLI-VKDNIEKKLQQRL 409981
Query: 192 TPLMVKGFRDAAEEGGTAVTGGQT 215
++ + EE ++GG T
Sbjct: 409980 QNILTGCCQKLKEE-KCVLSGGHT 409912
Score = 30.8 bits (68), Expect = 1.8, Method: Composition-based stats.
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -1
Query: 300 RTAAGLMHTF-NAHAATDITGFGILGHSQNLAKQQRN 335
RT G +H NA +I GIL H QN+ + RN
Sbjct: 345704 RTNGGYLHNHRNAKKKINIINMGILTHIQNIPAESRN 345594
Database: genoma.fa
Posted date: Mar 4, 2008 2:57 PM
Number of letters in database: 23,462,190
Number of sequences in database: 14
Lambda K H
0.317 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 11,103,149
Number of extensions: 168829
Number of successful extensions: 570
Number of sequences better than 10.0: 1
Number of HSP's gapped: 569
Number of HSP's successfully gapped: 3
Length of query: 448
Length of database: 7,820,730
Length adjustment: 101
Effective length of query: 347
Effective length of database: 7,819,316
Effective search space: 2713302652
Effective search space used: 2713302652
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SPP00000033_1.0 # Protein # Selenophosphate synthetase 2 (SPS2) # Homo sapiens # Complete
# Database: genoma.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SPP00000033_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 30.39 102 71 2 241 342 409614 409351 5e-06 48.9
SPP00000033_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 29.76 84 59 2 132 215 410157 409912 1e-04 44.7
SPP00000033_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 40.54 37 21 1 300 335 345704 345594 1.8 30.8
