TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPS1_nostra
         (419 letters)

Database: cds.fa 
           5106 sequences; 11,140,562 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_070780|Annotation|Pla...   432   e-122
Plasmodium_knowlesi_strain_H|PK4.chr03|PKH_030600|Annotation|Pla...    32   0.40 
Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_133400|Annotation|Pla...    29   2.4  

>Plasmodium_knowlesi_strain_H|PK4.chr07|PKH_070780|Annotation|Plasmodi
            um_knowlesi_Sanger|(protein coding) selenophosphate
            synthase, putative
          Length = 3450

 Score =  432 bits (1110), Expect = e-122,   Method: Composition-based stats.
 Identities = 212/212 (100%), Positives = 212/212 (100%)
 Frame = +1

Query: 13   LLVSKETAYRVYQEEGENGTTITIIVQDGSVHKGTPSHSDTKREKVNSYIKESIEKIINR 72
            LLVSKETAYRVYQEEGENGTTITIIVQDGSVHKGTPSHSDTKREKVNSYIKESIEKIINR
Sbjct: 1810 LLVSKETAYRVYQEEGENGTTITIIVQDGSVHKGTPSHSDTKREKVNSYIKESIEKIINR 1989

Query: 73   NTCGGCGSKVPSNVLSNSLKGLSVYNSPNVFLGIEACDDCCIFVHSKSKRGEESPALVQT 132
            NTCGGCGSKVPSNVLSNSLKGLSVYNSPNVFLGIEACDDCCIFVHSKSKRGEESPALVQT
Sbjct: 1990 NTCGGCGSKVPSNVLSNSLKGLSVYNSPNVFLGIEACDDCCIFVHSKSKRGEESPALVQT 2169

Query: 133  IDFFKSFIDDEYILGEIIAIHCLSDVYSMGGTGICALCVLIVKDNIEKKLQQRLQNILTG 192
            IDFFKSFIDDEYILGEIIAIHCLSDVYSMGGTGICALCVLIVKDNIEKKLQQRLQNILTG
Sbjct: 2170 IDFFKSFIDDEYILGEIIAIHCLSDVYSMGGTGICALCVLIVKDNIEKKLQQRLQNILTG 2349

Query: 193  CCQKLKEEKCVLSGGHTCAGNENYVGLAVTGK 224
            CCQKLKEEKCVLSGGHTCAGNENYVGLAVTGK
Sbjct: 2350 CCQKLKEEKCVLSGGHTCAGNENYVGLAVTGK 2445



 Score =  267 bits (683), Expect = 3e-72,   Method: Composition-based stats.
 Identities = 130/138 (94%), Positives = 132/138 (95%)
 Frame = +1

Query: 224  KENYLFLPKGSGSVKAGDIIITTKMFGFGFIMAAHICKKAKARWIYICLDEMLLSNRKSG 283
            KENYLFLPKGSGSVKAGDIIITTKMFGFGFIMAAHICKKAKARWIYICLDEMLLSNRKSG
Sbjct: 2662 KENYLFLPKGSGSVKAGDIIITTKMFGFGFIMAAHICKKAKARWIYICLDEMLLSNRKSG 2841

Query: 284  LYLLQNNNAKACTDVTGFGILGHLNEMIKCSRREIYFASHMKKKSLHTTSQRKILEGRNK 343
            LYLLQNNNAKACTDVTGFGILGHLNEMIKCSRREIYFASHMKKKSLHTTSQRKILEGRNK
Sbjct: 2842 LYLLQNNNAKACTDVTGFGILGHLNEMIKCSRREIYFASHMKKKSLHTTSQRKILEGRNK 3021

Query: 344  RMDDGENDQTSGGLMAIV 361
            RMDDGENDQ     M ++
Sbjct: 3022 RMDDGENDQVKEPPMNLI 3075



 Score =  140 bits (353), Expect = 5e-34,   Method: Composition-based stats.
 Identities = 68/68 (100%), Positives = 68/68 (100%)
 Frame = +1

Query: 352  QTSGGLMAIVERERAHQILADLKNMGYSNCSAVGEIINVQDYKFKGVPINQVSLDDYLDT 411
            QTSGGLMAIVERERAHQILADLKNMGYSNCSAVGEIINVQDYKFKGVPINQVSLDDYLDT
Sbjct: 3244 QTSGGLMAIVERERAHQILADLKNMGYSNCSAVGEIINVQDYKFKGVPINQVSLDDYLDT 3423

Query: 412  TNSVYIEC 419
            TNSVYIEC
Sbjct: 3424 TNSVYIEC 3447


>Plasmodium_knowlesi_strain_H|PK4.chr03|PKH_030600|Annotation|Plasmodi
            um_knowlesi_Sanger|(protein coding) Sporozoite protein
            with MAC/Perforin domain
          Length = 2535

 Score = 31.6 bits (70), Expect = 0.40,   Method: Composition-based stats.
 Identities = 17/56 (30%), Positives = 29/56 (51%)
 Frame = +1

Query: 84   SNVLSNSLKGLSVYNSPNVFLGIEACDDCCIFVHSKSKRGEESPALVQTIDFFKSF 139
            +    N++ GL     P  F+G+EA  +C   V+ + K  EE  ++   I FFK++
Sbjct: 1159 TTAFKNAVDGL-----PPHFIGLEAESECASDVYEQKKTSEECESVHAWITFFKTY 1311


>Plasmodium_knowlesi_strain_H|PK4.chr13|PKH_133400|Annotation|Plasmo
           dium_knowlesi_Sanger|(protein coding) hypothetical
           protein, conserved in Plasmodium species
          Length = 1908

 Score = 28.9 bits (63), Expect = 2.4,   Method: Composition-based stats.
 Identities = 17/58 (29%), Positives = 27/58 (46%)
 Frame = +1

Query: 191 TGCCQKLKEEKCVLSGGHTCAGNENYVGLAVTGKENYLFLPKGSGSVKAGDIIITTKM 248
           T C +  +EEK  ++ G     NEN   +    K+  +     S   +A DI++TT M
Sbjct: 607 TSCVKFAEEEKRNMNNGRWFGNNENQTNIPRRCKDQVMLPYSSSKDNEAADILLTTPM 780


  Database: cds.fa
    Posted date:  Mar 7, 2008  5:38 PM
  Number of letters in database: 11,140,562
  Number of sequences in database:  5106
  
Lambda     K      H
   0.319    0.136    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 5106
Number of Hits to DB: 5,449,989
Number of extensions: 88475
Number of successful extensions: 516
Number of sequences better than 10.0: 6
Number of HSP's gapped: 516
Number of HSP's successfully gapped: 8
Length of query: 419
Length of database: 3,713,520
Length adjustment: 95
Effective length of query: 324
Effective length of database: 3,228,450
Effective search space: 1046017800
Effective search space used: 1046017800
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 40 (20.0 bits)