TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= SPP00000032_1.0 # Protein # Selenophosphate synthetase 1 (SPS1)
# Homo sapiens # Complete
(392 letters)
Database: genoma.fa
14 sequences; 23,462,190 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmod... 49 6e-06
Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmod... 30 1.7
Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds... 29 5.7
>Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 1496036
Score = 48.5 bits (114), Expect = 6e-06, Method: Composition-based stats.
Identities = 47/169 (27%), Positives = 76/169 (44%), Gaps = 6/169 (3%)
Frame = -3
Query: 2 STRESFNPESYELDKSFRLTRFTELKGTGCKVPQDVLQKLLESLQENHFQEDEQFLGAVM 61
+ RE N SY + ++ G G KVP +VL L+ L + + FLG
Sbjct: 410382 TKREKVN--SYIKESIEKIINRNTCGGCGSKVPSNVLSNSLKGL--SVYNSPNVFLGIE- 410218
Query: 62 PRLGIGMDTCVIPL----RHGGLS--LVQTTDYIYPIVDDPYMMGRIACANVLSDLYAMG 115
D C I + + G S LVQT D+ +DD Y++G I + LSD+Y+MG
Sbjct: 410217 -----ACDDCCIFVHSKSKRGEESPALVQTIDFFKSFIDDEYILGEIIAIHCLSDVYSMG 410053
Query: 116 VTECDNMLMLLGVSNKMTDRERDKVMPLIIQGFKDAAEEAGTSVTGGQT 164
T + +L+ N +++ + + I+ G +E ++GG T
Sbjct: 410052 GTGICALCVLIVKDN--IEKKLQQRLQNILTGCCQKLKEEKCVLSGGHT 409912
Score = 47.8 bits (112), Expect = 1e-05, Method: Composition-based stats.
Identities = 31/98 (31%), Positives = 44/98 (44%)
Frame = -3
Query: 194 GDVLVLTKPLGTQVAVAVHQWLDIPEKWNKIKLVVTQEDVELAYQEAMMNMARLNRTAAG 253
GD+++ TK G +A H +W I L + M+ R ++
Sbjct: 409602 GDIIITTKMFGFGFIMAAHICKKAKARWIYICL------------DEMLLSNR--KSGLY 409465
Query: 254 LMHTFNAHAATDITGFGILGHAQNLAKQQRNEVSFVIH 291
L+ NA A TD+TGFGILGH + K R E+ F H
Sbjct: 409464 LLQNNNAKACTDVTGFGILGHLNEMIKCSRREIYFASH 409351
Score = 30.8 bits (68), Expect = 1.4, Method: Composition-based stats.
Identities = 15/37 (40%), Positives = 19/37 (51%), Gaps = 1/37 (2%)
Frame = -1
Query: 249 RTAAGLMHTF-NAHAATDITGFGILGHAQNLAKQQRN 284
RT G +H NA +I GIL H QN+ + RN
Sbjct: 345704 RTNGGYLHNHRNAKKKINIINMGILTHIQNIPAESRN 345594
Score = 29.6 bits (65), Expect = 3.0, Method: Composition-based stats.
Identities = 16/46 (34%), Positives = 28/46 (60%)
Frame = -3
Query: 300 AAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKY 345
A++S+ G +F P+TSGGL+ + RE+A + A++K+ Y
Sbjct: 409107 ASLSERYGLLFD------PQTSGGLMAIVERERAHQILADLKNMGY 408988
>Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2200295
Score = 30.4 bits (67), Expect = 1.7, Method: Composition-based stats.
Identities = 21/58 (36%), Positives = 27/58 (46%)
Frame = +3
Query: 297 AKMAAVSKACGNMFGLMHGTCPETSGGLLICLPREQAARFCAEIKSPKYGEGHQAWII 354
K + VS+ C N+F L LLIC PR++AA KYGE Q + I
Sbjct: 747423 GKASCVSR-CPNLFSLF--------SKLLICTPRDEAA---------KYGEAEQTFKI 747542
>Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2372884
Score = 28.9 bits (63), Expect = 5.7, Method: Composition-based stats.
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +1
Query: 288 FVIHNLPVLAKMAAVSKACGNMFGLMHGT 316
F +H + +LA + V+KAC +FG +H T
Sbjct: 1686832 FFVHVVRLLALPSFVAKACALLFGFLHYT 1686918
Database: genoma.fa
Posted date: Mar 7, 2008 5:34 PM
Number of letters in database: 23,462,190
Number of sequences in database: 14
Lambda K H
0.320 0.135 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 9,812,666
Number of extensions: 141669
Number of successful extensions: 427
Number of sequences better than 10.0: 4
Number of HSP's gapped: 427
Number of HSP's successfully gapped: 7
Length of query: 392
Length of database: 7,820,730
Length adjustment: 100
Effective length of query: 292
Effective length of database: 7,819,330
Effective search space: 2283244360
Effective search space used: 2283244360
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SPP00000032_1.0 # Protein # Selenophosphate synthetase 1 (SPS1) # Homo sapiens # Complete
# Database: genoma.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SPP00000032_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 27.81 169 116 6 2 164 410382 409912 6e-06 48.5
SPP00000032_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 31.63 98 67 2 194 291 409602 409351 1e-05 47.8
SPP00000032_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 40.54 37 21 1 249 284 345704 345594 1.4 30.8
SPP00000032_1.0 Plasmodium_knowlesi_strain_H|PK4.chr07|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 34.78 46 30 1 300 345 409107 408988 3.0 29.6
SPP00000032_1.0 Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 36.21 58 37 3 297 354 747423 747542 1.7 30.4
SPP00000032_1.0 Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 41.38 29 17 0 288 316 1686832 1686918 5.7 28.9
