BLASTP 2.2.17 [Aug-26-2007] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= kinasedomain_dsp (286 letters) Database: orfs.fa 176,078 sequences; 18,685,909 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Plasmodium_k||chr02-6-405575-404370|computed|PlasmoDB|length=402 31 0.28 Plasmodium_k||PK4.chr09-6-1618072-1616432|computed|PlasmoDB|leng... 31 0.30 Plasmodium_k||PK4.chr12.pseudo-3-705576-705749|computed|PlasmoDB... 29 1.2 Plasmodium_k||chr04-5-309467-308547|computed|PlasmoDB|length=307 28 1.6 Plasmodium_k||PK4.chr11.pseudo.embl-6-727400-727167|computed|Pla... 28 1.6 Plasmodium_k||chr14-2-2311154-2311378|computed|PlasmoDB|length=75 28 1.8 Plasmodium_k||PK4.chr12.pseudo-2-1955309-1955473|computed|Plasmo... 28 2.6 Plasmodium_k||PK4.chr12.pseudo-5-2012630-2010117|computed|Plasmo... 27 3.5 Plasmodium_k||PK4.chr09-1-81460-83400|computed|PlasmoDB|length=647 27 4.3 Plasmodium_k||chr14-5-2152544-2152215|computed|PlasmoDB|length=110 27 4.6 Plasmodium_k||PK4.chr13-5-641074-639572|computed|PlasmoDB|length... 27 5.0 Plasmodium_k||PK4.chr13-2-125039-126745|computed|PlasmoDB|length... 27 6.0 Plasmodium_k||PK4.chr11.pseudo.embl-2-1519907-1520176|computed|P... 26 7.8 >Plasmodium_k||chr02-6-405575-404370|computed|PlasmoDB|length=402 Length = 402 Score = 30.8 bits (68), Expect = 0.28, Method: Composition-based stats. Identities = 19/45 (42%), Positives = 24/45 (53%), Gaps = 3/45 (6%) Query: 76 QVRRISSSGDYNSGRH-LILCDDNFYYRSMRYKLYQLCRDSGCIF 119 Q +R SS H LIL +D F+ SMR K Y LCR C++ Sbjct: 359 QNQRCSSRQCRRKTEHVLILLNDTFHLPSMRKKYYLLCRK--CMY 401 >Plasmodium_k||PK4.chr09-6-1618072- 1616432|computed|PlasmoDB|length=547 Length = 547 Score = 30.8 bits (68), Expect = 0.30, Method: Composition-based stats. Identities = 18/82 (21%), Positives = 42/82 (51%), Gaps = 1/82 (1%) Query: 123 YMASSLDSCLQANSLRSDATRVPVDVVRQMNERLEVPDTSEAWERNSLTLNGLDMDTTGS 182 + ++S C+ NS++ AT + + +R+E PD E W + + L+ + ++ Sbjct: 52 FSSASQIECVDENSIKKLATVLRKKKNKNERDRMEHPDNPEKWVMSEVDLDEILVNIKNL 111 Query: 183 ALLA-FIASLLDLPAMETTLDL 203 ++ + +S+++ ME +DL Sbjct: 112 SVCSNLYSSMVESEIMEDIIDL 133 >Plasmodium_k||PK4.chr12.pseudo-3-705576- 705749|computed|PlasmoDB|length=58 Length = 58 Score = 28.9 bits (63), Expect = 1.2, Method: Composition-based stats. Identities = 11/18 (61%), Positives = 14/18 (77%) Query: 75 PQVRRISSSGDYNSGRHL 92 PQVR++S+ G NSGR L Sbjct: 36 PQVRKVSTKGKTNSGRTL 53 >Plasmodium_k||chr04-5-309467-308547|computed|PlasmoDB|length=307 Length = 307 Score = 28.5 bits (62), Expect = 1.6, Method: Composition-based stats. Identities = 20/69 (28%), Positives = 30/69 (43%), Gaps = 23/69 (33%) Query: 30 IVHLCYDDFLDATPSADLAYKEQRGRIFKVIEKLISAIQEDTDWPPQVRRISSSGDYNSG 89 I+HL DDF +KE+ G + ++ +KL A+ + SG +SG Sbjct: 66 ILHLIQDDF----------WKEETGEVVQLWQKLSEAMAQ-------------SGGQDSG 102 Query: 90 RHLILCDDN 98 H DDN Sbjct: 103 DHCNTVDDN 111 >Plasmodium_k||PK4.chr11.pseudo.embl-6-727400- 727167|computed|PlasmoDB|length=78 Length = 78 Score = 28.5 bits (62), Expect = 1.6, Method: Composition-based stats. Identities = 17/75 (22%), Positives = 35/75 (46%), Gaps = 2/75 (2%) Query: 27 VRHIVHLCYDDFLDATPSADLAYKEQRGRIFKVIEKLISAIQEDTDWPPQVRRISSSGDY 86 V+ V +C+ F+ S+D + G + KV+ L+ ++ D D P + SS+ Sbjct: 5 VKFRVSICWLPFMYRQNSSD--FMSSVGILNKVVSLLMVCLRMDLDTPSSRKLTSSTSTR 62 Query: 87 NSGRHLILCDDNFYY 101 + ++ CD + + Sbjct: 63 EENKFMLTCDSSVSF 77 >Plasmodium_k||chr14-2-2311154-2311378|computed|PlasmoDB|length=75 Length = 75 Score = 28.1 bits (61), Expect = 1.8, Method: Composition-based stats. Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 12/63 (19%) Query: 66 AIQEDTDWP----PQVR--------RISSSGDYNSGRHLILCDDNFYYRSMRYKLYQLCR 113 AIQ + P P++R RISS ++ H+ +C NF+ S L+ LC Sbjct: 6 AIQAEFSSPSFVIPRIRLKSPTGTCRISSVIHTHTHTHVCVCAGNFFVFSKSILLFPLCG 65 Query: 114 DSG 116 SG Sbjct: 66 QSG 68 >Plasmodium_k||PK4.chr12.pseudo-2-1955309- 1955473|computed|PlasmoDB|length=55 Length = 55 Score = 27.7 bits (60), Expect = 2.6, Method: Composition-based stats. Identities = 10/18 (55%), Positives = 14/18 (77%) Query: 71 TDWPPQVRRISSSGDYNS 88 TD+PPQ+ ++SS G NS Sbjct: 19 TDYPPQMTQLSSKGKRNS 36 >Plasmodium_k||PK4.chr12.pseudo-5-2012630- 2010117|computed|PlasmoDB|length=838 Length = 838 Score = 27.3 bits (59), Expect = 3.5, Method: Composition-based stats. Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 4/54 (7%) Query: 49 YKEQRGRIFKVIEKLISAIQE----DTDWPPQVRRISSSGDYNSGRHLILCDDN 98 YK RGR KV+ K I+E D D +++R++ G++ +L +DN Sbjct: 740 YKSYRGRTAKVLRKKKKLIEELDRLDPDRRQEMKRLTLQGEFAQLDNLRNFNDN 793 >Plasmodium_k||PK4.chr09-1-81460-83400|computed|PlasmoDB|length=647 Length = 647 Score = 26.9 bits (58), Expect = 4.3, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 19/34 (55%) Query: 249 LNSRRKDILTKFRTEIGGKQIDGDVEGDALDYYV 282 +N KD+L K++ E+ G ++G DYY+ Sbjct: 175 INESMKDVLEKYKNELTSLHFWGILKGVKKDYYI 208 >Plasmodium_k||chr14-5-2152544-2152215|computed|PlasmoDB|length=110 Length = 110 Score = 26.9 bits (58), Expect = 4.6, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 2/47 (4%) Query: 196 AMETTLDLPPAVARGLRQDQSLVHRLDLLLRTRIGALLDGLRQGEDK 242 AMET+L LPP A SL+ L+ + R+ ++ +R+ DK Sbjct: 21 AMETSLTLPPIYAHIYMYFSSLLKELNDIFRS--NDIVGDMRKNSDK 65 >Plasmodium_k||PK4.chr13-5-641074- 639572|computed|PlasmoDB|length=501 Length = 501 Score = 26.6 bits (57), Expect = 5.0, Method: Composition-based stats. Identities = 12/21 (57%), Positives = 16/21 (76%) Query: 1 VALIGLPAAGKSSLCSWLLGQ 21 +ALIG P AGKSSL + +L + Sbjct: 109 IALIGAPNAGKSSLLNSILNK 129 >Plasmodium_k||PK4.chr13-2-125039- 126745|computed|PlasmoDB|length=569 Length = 569 Score = 26.6 bits (57), Expect = 6.0, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 18/39 (46%) Query: 2 ALIGLPAAGKSSLCSWLLGQQAALRVRHIVHLCYDDFLD 40 A+ G GKSSLC+ + + H HL Y F D Sbjct: 79 AITGRRNTGKSSLCAHICVNLFFNDILHYFHLFYSSFFD 117 >Plasmodium_k||PK4.chr11.pseudo.embl-2-1519907- 1520176|computed|PlasmoDB|length=90 Length = 90 Score = 26.2 bits (56), Expect = 7.8, Method: Composition-based stats. Identities = 15/55 (27%), Positives = 22/55 (40%) Query: 188 IASLLDLPAMETTLDLPPAVARGLRQDQSLVHRLDLLLRTRIGALLDGLRQGEDK 242 I L+ LPA + LPP ++ Q VH R LL + G+ + Sbjct: 1 IGELISLPARKQQGKLPPDAIHVSKRTQIYVHTFSTAQRLAKSCLLTSISNGQKQ 55 Database: orfs.fa Posted date: Mar 12, 2008 4:11 PM Number of letters in database: 18,685,909 Number of sequences in database: 176,078 Lambda K H 0.321 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 176078 Number of Hits to DB: 8,994,727 Number of extensions: 301817 Number of successful extensions: 643 Number of sequences better than 10.0: 16 Number of HSP's gapped: 643 Number of HSP's successfully gapped: 16 Length of query: 286 Length of database: 18,685,909 Length adjustment: 90 Effective length of query: 196 Effective length of database: 2,838,889 Effective search space: 556422244 Effective search space used: 556422244 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 55 (25.8 bits) # BLASTP 2.2.17 [Aug-26-2007] # Query: kinasedomain_dsp # Database: orfs.fa # Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score kinasedomain_dsp Plasmodium_k||chr02-6-405575-404370|computed|PlasmoDB|length=402 42.22 45 23 2 76 119 359 401 0.28 30.8 kinasedomain_dsp Plasmodium_k||PK4.chr09-6-1618072-1616432|computed|PlasmoDB|length=547 21.95 82 63 1 123 203 52 133 0.30 30.8 kinasedomain_dsp Plasmodium_k||PK4.chr12.pseudo-3-705576-705749|computed|PlasmoDB|length=58 61.11 18 7 0 75 92 36 53 1.2 28.9 kinasedomain_dsp Plasmodium_k||chr04-5-309467-308547|computed|PlasmoDB|length=307 28.99 69 26 2 30 98 66 111 1.6 28.5 kinasedomain_dsp Plasmodium_k||PK4.chr11.pseudo.embl-6-727400-727167|computed|PlasmoDB|length=78 22.67 75 56 1 27 101 5 77 1.6 28.5 kinasedomain_dsp Plasmodium_k||chr14-2-2311154-2311378|computed|PlasmoDB|length=75 31.75 63 31 2 66 116 6 68 1.8 28.1 kinasedomain_dsp Plasmodium_k||PK4.chr12.pseudo-2-1955309-1955473|computed|PlasmoDB|length=55 55.56 18 8 0 71 88 19 36 2.6 27.7 kinasedomain_dsp Plasmodium_k||PK4.chr12.pseudo-5-2012630-2010117|computed|PlasmoDB|length=838 31.48 54 33 1 49 98 740 793 3.5 27.3 kinasedomain_dsp Plasmodium_k||PK4.chr09-1-81460-83400|computed|PlasmoDB|length=647 32.35 34 23 0 249 282 175 208 4.3 26.9 kinasedomain_dsp Plasmodium_k||chr14-5-2152544-2152215|computed|PlasmoDB|length=110 34.04 47 29 1 196 242 21 65 4.6 26.9 kinasedomain_dsp Plasmodium_k||PK4.chr13-5-641074-639572|computed|PlasmoDB|length=501 57.14 21 9 0 1 21 109 129 5.0 26.6 kinasedomain_dsp Plasmodium_k||PK4.chr13-2-125039-126745|computed|PlasmoDB|length=569 35.90 39 25 0 2 40 79 117 6.0 26.6 kinasedomain_dsp Plasmodium_k||PK4.chr11.pseudo.embl-2-1519907-1520176|computed|PlasmoDB|length=90 27.27 55 40 0 188 242 1 55 7.8 26.2