TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= pstk_Drosophila_melanogaster
(292 letters)
Database: genoma.fa
14 sequences; 23,462,190 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-DNA|Plasmodium_... 31 0.96
Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmod... 30 1.1
Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_... 28 5.4
>Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 726886
Score = 30.8 bits (68), Expect = 0.96, Method: Composition-based stats.
Identities = 21/49 (42%), Positives = 24/49 (48%), Gaps = 1/49 (2%)
Frame = -3
Query: 82 QVRRISSSGDYNSGRH-LILCDDNFYYRSMRYKLYQLCRDSGCIFGQIY 129
Q +R SS H LIL +D F+ SMR K Y LCR I IY
Sbjct: 404501 QNQRCSSRQCRRKTEHVLILLNDTFHLPSMRKKYYLLCRKCMYI*PYIY 404355
>Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 2147124
Score = 30.4 bits (67), Expect = 1.1, Method: Composition-based stats.
Identities = 18/82 (21%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = -3
Query: 129 YMASSLDSCLQANSLRSDATRVPVDVVRQMNERLEVPDTSEAWERNSLTLNGLDMDTTGS 188
+ ++S C+ NS++ AT + + +R+E PD E W + + L+ + ++
Sbjct: 1617919 FSSASQIECVDENSIKKLATVLRKKKNKNERDRMEHPDNPEKWVMSEVDLDEILVNIKNL 1617740
Query: 189 ALLA-FIASLLDLPAMETTLDL 209
++ + +S+++ ME +DL
Sbjct: 1617739 SVCSNLYSSMVESEIMEDIIDL 1617674
>Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-
DNA|Plasmodium_knowlesi_Sanger
Length = 3159096
Score = 28.1 bits (61), Expect = 5.4, Method: Composition-based stats.
Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 12/63 (19%)
Frame = +2
Query: 72 AIQEDTDWP----PQVR--------RISSSGDYNSGRHLILCDDNFYYRSMRYKLYQLCR 119
AIQ + P P++R RISS ++ H+ +C NF+ S L+ LC
Sbjct: 2311169 AIQAEFSSPSFVIPRIRLKSPTGTCRISSVIHTHTHTHVCVCAGNFFVFSKSILLFPLCG 2311348
Query: 120 DSG 122
SG
Sbjct: 2311349 QSG 2311357
Database: genoma.fa
Posted date: Mar 11, 2008 12:17 PM
Number of letters in database: 23,462,190
Number of sequences in database: 14
Lambda K H
0.322 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 7,237,971
Number of extensions: 104860
Number of successful extensions: 529
Number of sequences better than 10.0: 4
Number of HSP's gapped: 529
Number of HSP's successfully gapped: 5
Length of query: 292
Length of database: 7,820,730
Length adjustment: 97
Effective length of query: 195
Effective length of database: 7,819,372
Effective search space: 1524777540
Effective search space used: 1524777540
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
# TBLASTN 2.2.17 [Aug-26-2007]
# Query: pstk_Drosophila_melanogaster
# Database: genoma.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
pstk_Drosophila_melanogaster Plasmodium_knowlesi_strain_H|chr02|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 42.86 49 27 1 82 129 404501 404355 0.96 30.8
pstk_Drosophila_melanogaster Plasmodium_knowlesi_strain_H|PK4.chr09|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 21.95 82 63 1 129 209 1617919 1617674 1.1 30.4
pstk_Drosophila_melanogaster Plasmodium_knowlesi_strain_H|chr14|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger 31.75 63 31 2 72 122 2311169 2311357 5.4 28.1
