GPx7

# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SPP00000010_1.0 # Protein # Glutathione peroxidase 7 (GPx7) # Homo sapiens # Complete
# Database: genoma_PlasmoDB.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SPP00000010_1.0	Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	33.33	72	48	0	26	97	511563	511348	1e-04	42.4
SPP00000010_1.0	Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	33.33	72	48	0	26	97	2538817	2539032	1e-04	42.4


TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000010_1.0 # Protein # Glutathione peroxidase 7 (GPx7) #
Homo sapiens # Complete
         (187 letters)

Database: genoma_PlasmoDB.fa 
           14 sequences; 23,462,190 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmod...    42   1e-04
Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|...    42   1e-04

>Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger
          Length = 2200295

 Score = 42.4 bits (98), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 24/72 (33%), Positives = 33/72 (45%)
 Frame = -3

Query: 26     YDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFP 85
              YD+    + G  V +  Y+  V L+VN AS+CG T +H   L QL   L      V  + 
Sbjct: 511563 YDYHVKKLDGTTVPMSTYKNKVLLIVNSASKCGLTRKHVDQLNQLHDRLNEQGLEVRHWT 511384

Query: 86     CNQFGQQEPDSN 97
                + GQ    SN
Sbjct: 511383 KYRRGQLHVPSN 511348


>Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger
          Length = 3128370

 Score = 42.4 bits (98), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 24/72 (33%), Positives = 33/72 (45%)
 Frame = +1

Query: 26      YDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFP 85
               YD+    + G  V +  Y+  V L+VN AS+CG T +H   L QL   L      V  + 
Sbjct: 2538817 YDYHVKKLDGTTVPMSTYKNKVLLIVNSASKCGLTRKHVDQLNQLHDRLNEQGLEVRHWT 2538996

Query: 86      CNQFGQQEPDSN 97
                 + GQ    SN
Sbjct: 2538997 KYRRGQLHVPSN 2539032


  Database: genoma_PlasmoDB.fa
    Posted date:  Feb 22, 2008 12:03 PM
  Number of letters in database: 23,462,190
  Number of sequences in database:  14
  
Lambda     K      H
   0.320    0.135    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 4,484,125
Number of extensions: 61432
Number of successful extensions: 260
Number of sequences better than 1.0e-02: 2
Number of HSP's gapped: 260
Number of HSP's successfully gapped: 2
Length of query: 187
Length of database: 7,820,730
Length adjustment: 92
Effective length of query: 95
Effective length of database: 7,819,442
Effective search space: 742846990
Effective search space used: 742846990
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)



GPx8

# TBLASTN 2.2.17 [Aug-26-2007]
# Query: SPP00000011_1.0 # Protein # Glutathione peroxidase 8 (GPx8) # Homo sapiens # Complete
# Database: genoma.fa
# Fields: Query id, Subject id, % identity, alignment length, mismatches, gap openings, q. start, q. end, s. start, s. end, e-value, bit score
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	44.44	54	30	1	40	93	2538802	2538954	1e-04	43.1
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	48.15	27	13	1	164	189	2539690	2539770	0.53	30.8
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	59.09	22	9	0	162	183	1727245	1727310	2.0	28.9
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	44.44	54	30	1	40	93	511578	511426	1e-04	43.1
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	48.15	27	13	1	164	189	510682	510602	0.43	31.2
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	26.39	72	53	2	138	209	469922	469743	8.6	26.9
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr01|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	38.71	31	19	0	79	109	238006	238098	3.9	28.1
SPP00000011_1.0	Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-DNA|Plasmodium_knowlesi_Sanger	44.00	25	14	0	102	126	1105440	1105366	5.9	27.3


TBLASTN 2.2.17 [Aug-26-2007]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= SPP00000011_1.0 # Protein # Glutathione peroxidase 8 (GPx8) #
Homo sapiens # Complete
         (209 letters)

Database: genoma.fa 
           14 sequences; 23,462,190 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-DNA|...    43   1e-04
Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-DNA|Plasmod...    43   1e-04
Plasmodium_knowlesi_strain_H|PK4.chr01|2007-02-22|ds-DNA|Plasmod...    28   3.9  
Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds...    27   5.9  

>Plasmodium_knowlesi_strain_H|PK4.chr12.pseudo|2007-02-22|ds-
               DNA|Plasmodium_knowlesi_Sanger
          Length = 3128370

 Score = 43.1 bits (100), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 24/54 (44%), Positives = 31/54 (57%)
 Frame = +1

Query: 40      LKPKINSFYAFEVKDAKGRTVSLEKYKGKVSLVVNVASDCQLTDRNYLGLKELH 93
               L+P I   Y + VK   G TV +  YK KV L+VN AS C LT ++   L +LH
Sbjct: 2538802 LRPSI---YDYHVKKLDGTTVPMSTYKNKVLLIVNSASKCGLTRKHVDQLNQLH 2538954



 Score = 30.8 bits (68), Expect = 0.53,   Method: Composition-based stats.
 Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
 Frame = +1

Query: 164     WNFWKYLVNPEGQVVKFWKPEE-PIEV 189
               WNF K+LVN  G VV ++ P   P+E+
Sbjct: 2539690 WNFGKFLVNRSGNVVSYFSPRTYPLEI 2539770



 Score = 28.9 bits (63), Expect = 2.0,   Method: Composition-based stats.
 Identities = 13/22 (59%), Positives = 15/22 (68%)
 Frame = +1

Query: 162     PRWNFWKYLVNPEGQVVKFWKP 183
               PRWN  K+LV  E + VKF KP
Sbjct: 1727245 PRWNISKWLVKEEYKSVKFSKP 1727310


>Plasmodium_knowlesi_strain_H|PK4.chr13|2007-02-22|ds-
              DNA|Plasmodium_knowlesi_Sanger
          Length = 2200295

 Score = 43.1 bits (100), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 24/54 (44%), Positives = 31/54 (57%)
 Frame = -3

Query: 40     LKPKINSFYAFEVKDAKGRTVSLEKYKGKVSLVVNVASDCQLTDRNYLGLKELH 93
              L+P I   Y + VK   G TV +  YK KV L+VN AS C LT ++   L +LH
Sbjct: 511578 LRPSI---YDYHVKKLDGTTVPMSTYKNKVLLIVNSASKCGLTRKHVDQLNQLH 511426



 Score = 31.2 bits (69), Expect = 0.43,   Method: Composition-based stats.
 Identities = 13/27 (48%), Positives = 18/27 (66%), Gaps = 1/27 (3%)
 Frame = -2

Query: 164    WNFWKYLVNPEGQVVKFWKPEE-PIEV 189
              WNF K+LVN  G VV ++ P   P+E+
Sbjct: 510682 WNFGKFLVNRSGNVVSYFSPRTYPLEI 510602



 Score = 26.9 bits (58), Expect = 8.6,   Method: Composition-based stats.
 Identities = 19/72 (26%), Positives = 36/72 (50%)
 Frame = -1

Query: 138    HKIKILGSEGEPAFRFLVDSSKKEPRWNFWKYLVNPEGQVVKFWKPEEPIEVIRPDIAAL 197
              H I +L  E   A +F     K   R      L+N EG++      +E ++++ PD+  +
Sbjct: 469922 HVISLLTYEHMEASKFYTFKFKSVIR------LINVEGEI------KEEVKIVVPDMDVI 469779

Query: 198    VRQVIIKKKEDL 209
              +R++  +K ED+
Sbjct: 469778 LRKLKNEKLEDI 469743


>Plasmodium_knowlesi_strain_H|PK4.chr01|2007-02-22|ds-
              DNA|Plasmodium_knowlesi_Sanger
          Length = 838594

 Score = 28.1 bits (61), Expect = 3.9,   Method: Composition-based stats.
 Identities = 12/31 (38%), Positives = 16/31 (51%)
 Frame = +1

Query: 79     CQLTDRNYLGLKELHKEFGPSHFSVLAFPCN 109
              C    R+++ L  LH     SH+S L  PCN
Sbjct: 238006 CNFRRRHHVPLHLLHIYLDSSHYSPLPVPCN 238098


>Plasmodium_knowlesi_strain_H|PK4.chr11.pseudo.embl|2007-02-22|ds-
               DNA|Plasmodium_knowlesi_Sanger
          Length = 2372884

 Score = 27.3 bits (59), Expect = 5.9,   Method: Composition-based stats.
 Identities = 11/25 (44%), Positives = 16/25 (64%)
 Frame = -2

Query: 102     SVLAFPCNQFGESEPRPSKEVESFA 126
               + L+F CN F +  P PSK VE+ +
Sbjct: 1105440 TTLSFRCNYFSKCSPSPSKGVENVS 1105366


  Database: genoma.fa
    Posted date:  Feb 29, 2008 11:22 AM
  Number of letters in database: 23,462,190
  Number of sequences in database:  14
  
Lambda     K      H
   0.321    0.139    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14
Number of Hits to DB: 6,669,270
Number of extensions: 106514
Number of successful extensions: 485
Number of sequences better than 10.0: 5
Number of HSP's gapped: 485
Number of HSP's successfully gapped: 9
Length of query: 209
Length of database: 7,820,730
Length adjustment: 93
Effective length of query: 116
Effective length of database: 7,819,428
Effective search space: 907053648
Effective search space used: 907053648
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)