TBLASTN 2.2.17 [Aug-26-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= SPP00000035_1.0 # Protein # Thioredoxin reductase 2 (TR2) #
Homo sapiens # Complete
(524 letters)
Database: genome
1422 sequences; 8,743,570 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AAEL01000190 [organism=Cryptosporidium hominis][strain=TU502][te... 455 e-128
>AAEL01000190 [organism=Cryptosporidium
hominis][strain=TU502][tech=wgs][chromosome=2]
Length = 13440
Score = 455 bits (1170), Expect = e-128, Method: Composition-based stats.
Identities = 224/474 (47%), Positives = 323/474 (68%), Gaps = 15/474 (3%)
Frame = +3
Query: 56 KEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQ-DAPNYG 114
KEAA+ G+KVA+ D+V+PS QGT+WGLGGTCVNVGC+PKKLMH +AL+ I DA +G
Sbjct: 8490 KEAAKYGKKVALFDFVKPSTQGTKWGLGGTCVNVGCVPKKLMHYSALISSSIHHDAQMFG 8669
Query: 115 WEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAKGG 174
+ + +W K+ E ++NH++ LN+ +R L+ V+Y N A +D H+V G
Sbjct: 8670 HKTSSSF--EWGKLVETLRNHIRMLNFSYRTGLRVGNVEYINALAKLIDPHSV-EYEDNG 8840
Query: 175 KEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECA 234
++ +++ +I++ATGGRP P + GA++Y ITSDDIF+L +SPGKTLV+GASY+ LE A
Sbjct: 8841 QKKTITSRYILLATGGRPSIPETVPGAIQYSITSDDIFFLSKSPGKTLVIGASYIGLETA 9020
Query: 235 GFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQV 294
GFL +G DTT+ MRSIPLRGFD+Q S ++E+M + GT+FL G P + ++ + ++V
Sbjct: 9021 GFLNELGFDTTVAMRSIPLRGFDRQCSEKIVEYMKATGTKFLVGVVPINIEKVNE-NIKV 9197
Query: 295 TWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHI 354
++ D + + F+TVL+A GR PD + LNL GV+ S D+ KI+ ++ATSVP I
Sbjct: 9198 SFSDGSVEE-----FETVLYATGRNPDVKGLNLNAVGVEVS-DSGKIIA-PKDATSVPSI 9356
Query: 355 YAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEE 414
+A+GD+VEGRPELTP+A+ AG LL +RLF GS++ +DYD VPTTVFTP+EYG VGLS E
Sbjct: 9357 FAVGDIVEGRPELTPVAVKAGILLARRLFAGSNEFIDYDFVPTTVFTPIEYGHVGLSSEA 9536
Query: 415 AVARHGQEHVEVYHAHYKPLEFTVAGRDASQ--------------CYVKMVCLREPPQLV 460
A+A++G++ +E Y + + LE A R+ + C K+V ++ + V
Sbjct: 9537 AIAKYGEDDIEEYLSEFSTLEIAAAHREKPEHLRENEMDFALPLNCLAKLVVVKSQGEKV 9716
Query: 461 LGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSG 514
+G HF+GPNAGE+TQGF+L +K GA+ +GIHPT +E L ++KRSG
Sbjct: 9717 VGFHFVGPNAGEITQGFSLAVKLGATKKDFDDMIGIHPTDAEVFGILEVTKRSG 9878
Database: genome
Posted date: Jan 30, 2008 8:57 PM
Number of letters in database: 8,743,570
Number of sequences in database: 1422
Lambda K H
0.321 0.137 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1422
Number of Hits to DB: 3,897,357
Number of extensions: 46838
Number of successful extensions: 164
Number of sequences better than 5.0e-02: 1
Number of HSP's gapped: 159
Number of HSP's successfully gapped: 1
Length of query: 524
Length of database: 2,914,523
Length adjustment: 95
Effective length of query: 429
Effective length of database: 2,779,433
Effective search space: 1192376757
Effective search space used: 1192376757
Neighboring words threshold: 13
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 45 (21.9 bits)