CLUSTAL W (1.83) multiple sequence alignment transcript2 GCCTTCCTGGGATTGGAGTCTCGAGCTTTCTTCGTTCGTTCGTCGGCGGGTTCGCGCCCT 60 transcript3 GCCTTCCTGGGATTGGAGTCTCGAGCTTTCTTCGTTCGTTCGTCGGCGGGTTCGCGCCCT 60 transcript1 GCCTTCCTGGGATTGGAGTCTCGAGCTTTCTTCGTTCGTTCGTCGGCGGGTTCGCGCCCT 60 ************************************************************ transcript2 TCTCGCGCCTCGGGGCTGCGAGGCTGGGGAAGGGGTTGGAGGGGGCTGTTGATCGCCGCG 120 transcript3 TCTCGCGCCTCGGGGCTGCGAGGCTGGGGAAGGGGTTGGAGGGGGCTGTTGATCGCCGCG 120 transcript1 TCTCGCGCCTCGGGGCTGCGAGGCTGGGGAAGGGGTTGGAGGGGGCTGTTGATCGCCGCG 120 ************************************************************ transcript2 TTTAAGTTGCGCTCGGGGCGGCCATGTCGGCCGGCGAGGTCGAGCGCCTAGTGTCGGAGC 180 transcript3 TTTAAGTTGCGCTCGGGGCGGCCATGTCGGCCGGCGAGGTCGAGCGCCTAGTGTCGGAGC 180 transcript1 TTTAAGTTGCGCTCGGGGCGGCCATGTCGGCCGGCGAGGTCGAGCGCCTAGTGTCGGAGC 180 ************************************************************ transcript2 TGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGTGGCTCTATGGCGGCCCATGGGACG 240 transcript3 TGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGTGGCTCTATGGCGGCCCATGGGACG 240 transcript1 TGAGCGGCGGGACCGGAGGGGATGAGGAGGAAGAGTGGCTCTATGGCG------------ 228 ************************************************ transcript2 TGCATGTGCACAGTGATTTGGCAAAGGACCTAGATGAAAATGAAGTTGAAAGGCCAGAAG 300 transcript3 TGCATGTGCACAGTGATTTGGCAAAGGACCTAGATGAAAATGAAGTTGAAAGGCCAGAAG 300 transcript1 ---------------------------------ATGAAAATGAAGTTGAAAGGCCAGAAG 255 *************************** transcript2 AAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGATGAAACTGCTGAAAATGGTG 360 transcript3 AAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGATGAAACTGCTGAAAATGGTG 360 transcript1 AAGAAAATGCCAGTGCTAATCCTCCATCTGGAATTGAAGATGAAACTGCTGAAAATGGTG 315 ************************************************************ transcript2 TACCAAAACCGAAAGTGACTGAGACCGAAGATGATAGTGATAGTGACAGCGATGATGATG 420 transcript3 TACCAAAACCGAAAGTGACTGAGACCGAAGATGATAGTGATAGTGACAGCGATGATGATG 420 transcript1 TACCAAAACCGAAAGTGACTGAGACCGAAGATGATAGTGATAGTGACAGCGATGATGATG 375 ************************************************************ transcript2 AAGATGATGTTCATGTCACTATAGGAGACATTAAAACGGGAGCACCACAGTATGGGAGTT 480 transcript3 AAGATGATGTTCATGTCACTATAGGAGACATTAAAACGGGAGCACCACAGTATGGGAGTT 480 transcript1 AAGATGATGTTCATGTCACTATAGGAGACATTAAAACGGGAGCACCACAGTATGGGAGTT 435 ************************************************************ transcript2 ATGGTACAGCACCTGTAAATCTTAACATCAAGACAGGGGGAAGAGTTTATGGAACTACAG 540 transcript3 ATGGTACAGCACCTGTAAATCTTAACATCAAGACAGGGGGAAGAGTTTATGGAACTACAG 540 transcript1 ATGGTACAGCACCTGTAAATCTTAACATCAAGACAGGGGGAAGAGTTTATGGAACTACAG 495 ************************************************************ transcript2 GGACAAAAGTCAAAGGAGTAGACCTTGATGCACCTGGAAGCATTAATGGAGTTCCACTCT 600 transcript3 GGACAAAAGTCAAAGGAGTAGACCTTGATGCACCTGGAAGCATTAATGGAGTTCCACTCT 600 transcript1 GGACAAAAGTCAAAGGAGTAGACCTTGATGCACCTGGAAGCATTAATGGAGTTCCACTCT 555 ************************************************************ transcript2 TAGAGGTAGATTTGGATTCTTTTGAAGATAAACCATGGCGTAAACCTGGTGCTGATCTTT 660 transcript3 TAGAGGTAGATTTGGATTCTTTTGAAGATAAACCATGGCGTAAACCTGGTGCTGATCTTT 660 transcript1 TAGAGGTAGATTTGGATTCTTTTGAAGATAAACCATGGCGTAAACCTGGTGCTGATCTTT 615 ************************************************************ transcript2 CTGATTATTTTAATTATGGGTTTAATGAAGATACCTGGAAAGCTTACTGTGAAAAACAAA 720 transcript3 CTGATTATTTTAATTATGGGTTTAATGAAGATACCTGGAAAGCTTACTGTGAAAAACAAA 720 transcript1 CTGATTATTTTAATTATGGGTTTAATGAAGATACCTGGAAAGCTTACTGTGAAAAACAAA 675 ************************************************************ transcript2 AGAGGATACGAATGGGACTTGAAGTTATACCAGTAACCTCTACTACAAATAAAATTACGG 780 transcript3 AGAGGATACGAATGGGACTTGAAGTTATACCAGTAACCTCTACTACAAATAAAATTACGG 780 transcript1 AGAGGATACGAATGGGACTTGAAGTTATACCAGTAACCTCTACTACAAATAAAATTACGG 735 ************************************************************ transcript2 CCGAAGACTGTACTATGGAAGTTACACCAGGTGCAGAGATCCAAGATGGCAGATTCAATC 840 transcript3 CCGAAGACTGTACTATGGAAGTTACACCAGGTGCAGAGATCCAAGATGGCAGATTCAATC 840 transcript1 ------------------------------------------------------------ transcript2 TTTTTAAGGTACAGCAGGGAAGAACTGGAAACTCAGAGAAAGAAACTGCCCTTCCATCTA 900 transcript3 TTTTTAAGGTACAGCAGGGAAGAACTGGAAACTCAGAGAAAGAAACTGCCCTTCCATCTA 900 transcript1 ---------TACAGCAGGGAAGAACTGGAAACTCAGAGAAAGAAACTGCCCTTCCATCTA 786 *************************************************** transcript2 CAAAAGCTGAGTTTACTTCTCCTCCTTCTTTGTTCAAGACTGGGCTTCCACCGAGCAGAA 960 transcript3 CAAAAGCTGAGTTTACTTCTCCTCCTTCTTTGTTCAAGACTGGGCTTCCACCGAGCAGAA 960 transcript1 CAAAAGCTGAGTTTACTTCTCCTCCTTCTTTGTTCAAGACTGGGCTTCCACCGAGCAG-- 844 ********************************************************** transcript2 ACAGCACTTCTTCTCAGTCTCAGACAAGTACTGCCTCCAGAAAAGCCAATTCAAGCGTTG 1020 transcript3 ACAGCACTTCTTCTCAGTCTCAGACAAGTACTGCCTCCAGAAAAGCCAATTCAAGCGTTG 1020 transcript1 ------------------------------------------------------------ transcript2 GGAAGTGGCAGGATCGATATGGGAGGGCCGAATCACCTGATCTAAGGAGATTACCTGGGG 1080 transcript3 GGAAGTGGCAGGATCGATATGGGAGGGCCGAATCACCTGATCTAAGGAGATTACCTGGGG 1080 transcript1 ----------------------------------------------GAGATTACCTGGGG 858 ************** transcript2 CAATTGATGTTATCGGTCAGACTATAACTATCAGCCGAGTAGAAGGCAGGCGACGGGCAA 1140 transcript3 CAATTGATGTTATCGGTCAGACTATAACTATCAGCCGAGTAGAAGGCAGGCGACGGGCAA 1140 transcript1 CAATTGATGTTATCGGTCAGACTATAACTATCAGCCGAGTAGAAGGCAGGCGACGGGCAA 918 ************************************************************ transcript2 ATGAGAACAGCAACATACAGGTCCTTTCTGAAAGATCTGCTACTGAAGTAGACAACAATT 1200 transcript3 ATGAGAACAGCAACATACAGGTCCTTTCTGAAAGATCTGCTACTGAAGTAGACAACAATT 1200 transcript1 ATGAGAACAGCAACATACAGGTCCTTTCTGAAAGATCTGCTACTGAAGTAGACAACAATT 978 ************************************************************ transcript2 TTAGCAAACCACCTCCGTTTTTCCCTCCAGGAGCTCCTCCCACTCACCTTCCACCTCCTC 1260 transcript3 TTAGCAAACCACCTCCGTTTTTCCCTCCAGGAGCTCCTCCCACTCACCTTCCACCTCCTC 1260 transcript1 TTAGCAAACCACCTCCGTTTTTCCCTCCAGGAGCTCCTCCCACTCACCTTCCACCTCCTC 1038 ************************************************************ transcript2 CATTTCTTCCACCTCCTCCGACTGTCAGCACTGCTCCACCTCTGATTCCACCACCGGGTT 1320 transcript3 CATTTCTTCCACCTCCTCCGACTGTCAGCACTGCTCCACCTCTGATTCCACCACCGGGTT 1320 transcript1 CATTTCTTCCACCTCCTCCGACTGTCAGCACTGCTCCACCTCTGATTCCACCACCGGGTT 1098 ************************************************************ transcript2 TTCCTCCTCCACCAGGCGCTCCACCTCCATCTCTTATACCAACAATAGAAAGTGGACATT 1380 transcript3 TTCCTCCTCCACCAGGCGCTCCACCTCCATCTCTTATACCAACAATAGAAAGTGGACATT 1380 transcript1 TTCCTCCTCCACCAGGCGCTCCACCTCCATCTCTTATACCAACAATAGAAAGTGGACATT 1158 ************************************************************ transcript2 CCTCTGGTTATGATAGTCGTTCTGCACGTGCATTTCCATATGGCAATGTTGCCTTTCCCC 1440 transcript3 CCTCTGGTTATGATAGTCGTTCTGCACGTGCATTTCCATATGGCAATGTTGCCTTTCCCC 1440 transcript1 CCTCTGGTTATGATAGTCGTTCTGCACGTGCATTTCCATATGGCAATGTTGCCTTTCCCC 1218 ************************************************************ transcript2 ATCTTCCTGGTTCTGCTCCTTCGTGGCCTAGTCTTGTGGACACCAGCAAGCAGTGGGACT 1500 transcript3 ATCTTCCTGGTTCTGCTCCTTCGTGGCCTAGTCTTGTGGACACCAGCAAGCAGTGGGACT 1500 transcript1 ATCTTCCTGGTTCTGCTCCTTCGTGGCCTAGTCTTGTGGACACCAGCAAGCAGTGGGACT 1278 ************************************************************ transcript2 ATTATGCCAGAAGAGAGAAAGACCGAGATAGAGAGAGAGACAGAGACAGAGAGCGAGACC 1560 transcript3 ATTATGCCAGAAGAGAGAAAGACCGAGATAGAGAGAGAGACAGAGACAGAGAGCGAGACC 1560 transcript1 ATTATGCCAGAAGAGAGAAAGACCGAGATAGAGAGAGAGACAGAGACAGAGAGCGAGACC 1338 ************************************************************ transcript2 GTGATCGGGACAGAGAAAGAGAACGCACCAGAGAGAGAGAGAGGGAGCGTGATCACAGTC 1620 transcript3 GTGATCGGGACAGAGAAAGAGAACGCACCAGAGAGAGAGAGAGGGAGCGTGATCACAGTC 1620 transcript1 GTGATCGGGACAGAGAAAGAGAACGCACCAGAGAGAGAGAGAGGGAGCGTGATCACAGTC 1398 ************************************************************ transcript2 CTACACCAAGTGTTTTCAACAGCGATGAAGAACGATACAGATACAGGGAATATGCAGAAA 1680 transcript3 CTACACCAAGTGTTTTCAACAGCGATGAAGAACGATACAGATACAGGGAATATGCAGAAA 1680 transcript1 CTACACCAAGTGTTTTCAACAGCGATGAAGAACGATACAGATACAGGGAATATGCAGAAA 1458 ************************************************************ transcript2 GAGGTTATGAGCGTCACAGAGCAAGTCGAGAAAAAGAAGAACGACATAGAGAAAGACGAC 1740 transcript3 GAGGTTATGAGCGTCACAGAGCAAGTCGAGAAAAAGAAGAACGACATAGAGAAAGACGAC 1740 transcript1 GAGGTTATGAGCGTCACAGAGCAAGTCGAGAAAAAGAAGAACGACATAGAGAAAGACGAC 1518 ************************************************************ transcript2 ACAGGGAGAAAGAGGAAACCAGACATAAGTCTTCTCGAAGTAATAGTAGACGTCGCCATG 1800 transcript3 ACAGGGAGAAAGAGGAAACCAGACATAAGTCTTCTCGAAGTAATAGTAGACGTCGCCATG 1800 transcript1 ACAGGGAGAAAGAGGAAACCAGACATAAGTCTTCTCGAAGTAATAGTAGACGTCGCCATG 1578 ************************************************************ transcript2 AAAGTGAAGAAGGAGATAGTCACAGGAGACACAAACACAAAAAATCTAAAAGAAGCAAAG 1860 transcript3 AAAGTGAAGAAGGAGATAGTCACAGGAGACACAAACACAAAAAATCTAAAAGAAGCAAAG 1860 transcript1 AAAGTGAAGAAGGAGATAGTCACAGGAGACACAAACACAAAAAATCTAAAAGAAGCAAAG 1638 ************************************************************ transcript2 AAGGAAAAGAAGCGGGCAGTGAGCCTGCCCCTGAACAGGAGAGCACCGAAGCTACACCTG 1920 transcript3 AAGGAAAAGAAGCGGGCAGTGAGCCTGCCCCTGAACAGGAGAGCACCGAAGCTACACCTG 1920 transcript1 AAGGAAAAGAAGCGGGCAGTGAGCCTGCCCCTGAACAGGAGAGCACCGAAGCTACACCTG 1698 ************************************************************ transcript2 CAGAATAGGCATGGTTTTGGCCTTTTGTGTATATTAGTACCAGAAGTAGATACTATAAAT 1980 transcript3 CAGAATAGGCATGGTTTTGGCCTTTTGTGTATATTAGTACCAGAAGTAGATACTATAAAT 1980 transcript1 CAGAATAGGCATGGTTTTGGCCTTTTGTGTATATTAGTACCAGAAGTAGATACTATAAAT 1758 ************************************************************ transcript2 CTTGTTATTTTTCTGGATAATGTTTAAGAAATTTACCTTAAATCTTGTTCTGTTTGTTAG 2040 transcript3 CTTGTTATTTTTCTGGATAATGTTTAAGAAATTTACCTTAAATCTTGTTCTGTTTGTTAG 2040 transcript1 CTTGTTATTTTTCTGGATAATGTTTAAGAAATTTACCTTAAATCTTGTTCTGTTTGTTAG 1818 ************************************************************ transcript2 TATGAAAAGTTAACTTTTTTTCCAAAATAAAAGAGTGAATTTTTCATGTTAAGTTAAAAA 2100 transcript3 TATGAAAAGTTAACTTTTTTTCCAAAATAAAAGAGTGAATTTTTCATGTTAAGTTAAAAA 2100 transcript1 TATGAAAAGTTAACTTTTTTTCCAAAATAAAAGAGTGAATTTTTCATGTTAAGTTAAAAA 1878 ************************************************************ transcript2 TCTTTGTCTTGTACTATTTCAAAAATAAAAAGACAGCAATGACTTTATATCCAA 2154 transcript3 ------------------------------------------------------ transcript1 TCTTTGTCTTGTACTATTTCAAAAATAAAAAGACAGCAATGACTTTATATCCAA 1932
Here we can see the alignment between the three transcripts of FIP1L1. As we have said before, the transcript number 1 doesn't have exons number 2, 9 and 11. We have marked in green the place where transcript 2 and 3 have exon 2 and transcript number 1 hasn't; in red the place where transcript 2 and 3 have exon 9 and transcript number 1 hasn't; and finally, in blue the place where transcript 2 and 3 have exon 11 and transcript number 1 hasn't. In yellow we have marked the absence of 54 nucleotides in transcript number 3, wheareas transcript number two has this 54 nucleotides.