CLUSTAL W (1.83) multiple sequence alignment Fusion --------------------------------MSAGEVERLVSELSGGTGGDEEEEWLYG 28 PDGFRA MGTSHPAFLVLGCLLTGLSLILCQLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQY- 59 FIP1L1 --------------------------------MSAGEVERLVSELSGGTGGDEEEEWLYG 28 . :* :* *.:* *:.* .* * Fusion GPWDVHVHSDLAKDLDENE----VERPEEENASANPP---------SGIEDETAEN---- 71 PDGFRA -PMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIY 118 FIP1L1 GPWDVHVHSDLAKDLDENE----VERPEEENASANPP---------SGIEDETAENG--- 72 * . . **: :**: * * ..*** . : *:: *. Fusion -GVPKPKVTET----EDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGG 126 PDGFRA IYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGF 178 FIP1L1 --VPKPKVTETE----DDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGG 126 **.*.*: . * .***. : * :* .. :.* *..*.. : : * Fusion -------------------------RVYGTTGTKVKGVDLDAPGSING-----VPLLEVD 156 PDGFRA NGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVF 238 FIP1L1 -------------------------RVYGTTGTKVKGVDLDAPGSING-----VPLLEVD 156 .**. ..*. .::::* :: * * Fusion LDSFEDKPWRKPG----------------------------ADLSDYFNYGFNEDTWKAY 188 PDGFRA NNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATRE 298 FIP1L1 LDSFEDKPWRKPG----------------------------ADLSDYFNYGFNEDTWKAY 188 :.. * * ** * :.* :* . Fusion CEKQKRIRMG------LEVIPVTS----------------------------TTNKITAE 214 PDGFRA VKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIE 358 FIP1L1 CEKQKRIRMG------LEVIPVTS----------------------------TTNKITAE 214 :: *:: :. :*: *. * ..* * Fusion DCTMEVTPGAEIQDGRFN----LFKVQQGRTGN----SEKETALPS-TKAEFTSPPSLFK 265 PDGFRA NLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSIL 418 FIP1L1 DCTMEVTPGAEIQDGRFN----LFKVQQGRTGN----SEKETALPS-TKAEFTSPPSLFK 265 : * .* :**: *:. *::.:: :*: :::* *: * * :*. ** : Fusion TGLPPSRNSTSSQSQTSTAS---------------RKAN--------------------- 289 PDGFRA DLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHS 478 FIP1L1 TGLPPSRNSTSSQSQTSTAS---------------RKAN--------------------- 289 : :.**..*: .**. :*.* Fusion ---SSVG----------------------KWQDRYGRAESPDLR---RLPGAIDVIGQTI 321 PDGFRA RDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSELTVAAAVLVLLVIV 538 FIP1L1 ---SSVG----------------------KWQDRYGRAESPDLR---RLPGAIDVIGQTI 321 *:* ::* : :* ** :..*: *: : Fusion TIS------------RVEGRRRANENSN-----------IQLPYDSRWEFPRDGLVLGRV 358 PDGFRA IISLIVLVVIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFPRDGLVLGRV 598 FIP1L1 TIS------------RVEGRRRANENSN-------------------------IQVLSER 344 ** * * * *. *. . **.. Fusion LGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGPHLNIV 418 PDGFRA LGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGPHLNIV 658 FIP1L1 SATEVDNNFSKPPPFFPPGAPPTHLPPPPFLPPPPTVSTAPPLIPPP------------- 391 .: . .:. : ..: . : *. . :* *.. * .*:. Fusion NLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEKPKKELDIFGLNPADESTR 478 PDGFRA NLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEKPKKELDIFGLNPADESTR 718 FIP1L1 --------------------------------------------------GFPPPPGAPP 401 *: *. :. Fusion SYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEV 538 PDGFRA SYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEV 778 FIP1L1 PSLIPTIESG-------------------------------------------------- 411 . :* ::*.. Fusion KNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQGKIVKICDFG 598 PDGFRA KNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQGKIVKICDFG 838 FIP1L1 ------------------------------------------------------------ Fusion LARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIFSLGGTPYPG 658 PDGFRA LARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIFSLGGTPYPG 898 FIP1L1 ------HSSGYDSRS----------------------ARAFPYGNVAFPHLPGSAPSWPS 443 *.*.* *:. : .:.** : : :. ....:*. Fusion MMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEIVENLLPGQY 718 PDGFRA MMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEIVENLLPGQY 958 FIP1L1 LVDTSKQWDYYAR-----REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVF 498 :: *. :: : .. * . : : : *:. : : * .. *. : Fusion KKSYEKIHLDFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLSADSGY 778 PDGFRA KKSYEKIHLDFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLSADSGY 1018 FIP1L1 NSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESE--- 555 :.. *: : . * :.:: : ::::*:. :. . . ..:* .:: Fusion IIPLPDIDPVPEEEDLGKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIDMMDDIGI 838 PDGFRA IIPLPDIDPVPEEEDLGKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIDMMDDIGI 1078 FIP1L1 ------------EGDSHRRHKHKKSKRSKEGKEAGSEPAPEQESTEATPAE--------- 594 * * :*::*.... .:.. *:**..:. : : * : Fusion DSSDLVEDSFL 849 PDGFRA DSSDLVEDSFL 1089 FIP1L1 -----------
As we have said before the FIP1L1-PDGFRA fusion protein is formed by the first part of the FIP1L1 protein and for the last part of the PDGFRA protein. Here with this alignment we can demonstrate that this fact occurs like that. In green you can see how the first part of FIP1L1 protein is equal than the first part of the fusion protein. In red you can see how the last part of the PDGFRA protein is equal than the last part of the fusion protein.