This version of the TRANSFAC database is free for users from non-profit organizations only.
Users from commercial organizations have to license the TRANSFAC databases and accompanying programs.
AC T00385
XX
ID T00385
XX
DT 15.10.1992 (created); ewi.
DT 12.11.2004 (updated); oke.
CO Copyright (C), Biobase GmbH.
XX
FA HSF1
XX
SY heat shock (transcription) factor; heat shock transcription factor; HSF; HSTF; YGL073W.
XX
OS yeast, Saccharomyces cerevisiae
OC Eukaryota; Fungi; Ascomycota; Hemiascomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
XX
GE G000932 HSF1.
XX
CL C0050 HSF; 3.4.1.0.8..
XX
SZ 833 AA; 93.3 kDa (cDNA), 130-150 kDa (SDS)
XX
SQ MNNAANTGTTNESNVSDAPRIEPLPSLNDDDIEKILQPNDIFTTDRTDASTTSSTAIEDI
SQ INPSLDPQSAASPVPSSSFFHDSRKPSTSTHLVRRGTPLGIYQTNLYGHNSRENTNPNST
SQ LLSSKLLAHPPVPYGQNPDLLQHAVYRAQPSSGTTNAQPRQTTRRYQSHKSRPAFVNKLW
SQ SMLNDDSNTKLIQWAEDGKSFIVTNREEFVHQILPKYFKHSNFASFVRQLNMYGWHKVQD
SQ VKSGSIQSSSDDKWQFENENFIRGREDLLEKIIRQKGSSNNHNSPSGNGNPANGSNIPLD
SQ NAAGSNNSNNNISSSNSFFNNGHLLQGKTLRLMNEANLGDKNDVTAILGELEQIKYNQIA
SQ ISKDLLRINKDNELLWQENMMARERHRTQQQALEKMFRFLTSIVPHLDPKMIMDGLGDPK
SQ VNNEKLNSANNIGLNRDNTGTIDELKSNDSFINDDRNSFTNATTNARNNMSPNNDDNSID
SQ TASTNTTNRKKNIDENIKNNNDIINDIIFNTNLANNLSNYNSNNNAGSPIRPYKQRYLLK
SQ NRANSSTSSENPSLTPFDIESNNDRKISEIPFDDEEEEETDFRPFTSRDPNNQTSENTFD
SQ PNRFTMLSDDDLKKDSHTNDNKHNESDLFWDNVHRNIDEQDARLQNLENMVHILSPGYPN
SQ KSFNNKTSSTNTNSNMESAVNVNSPGFNLQDYLTGESNSPNSVHSVPSNGSGSTPLPMPN
SQ DNDTEHASTSVNQGENGSGLTPFLTVDDHTLNDNNTSEGSTRVSPDIKFSATENTKVSDN
SQ LPSFNDHSYSTQADTAPENAKKRFVEEIPEPAIVEIQDPTEYNDHRLPKRAKK
XX
SC SwissProt #P10961
XX
FT 43 90 
serine-/threonine-rich region (20/48).
FT 167 284 
DNA-binding domain [9].
FT 171 275 
SM00415; HSF.
FT 173 416 
PF00447; HSF_DNA-bind.
FT 280 321 
PS50321; ASN_RICH_1.
FT 327 424 
trimerization through 2 alpha-helices forming a putative 3-stranded parallel coiled coil.
FT 422 525 
PS50321; ASN_RICH_2.
FT 569 581 
acidic region (9/13).
XX
SF phosphorylation [12] [10];
SF 2 activation domains within the N-terminus (for transient activity) and within the C-terminus (for sustained activity) [5];
SF at least one of them is masked under non-induced conditions [6];
SF trimerization through the hydrophobic heptad repeats of 2 alpha-helices that may form a 3-stranded parallel coiled-coil [7];
SF DNA-binding and oligomerization domain determine temperature-dependent regulation [3];
XX
FF activator, essential for growth, mediating expression of heat-shock genes in response to elevated temperatures [9] [10];
FF activates gene expression in response to stress conditions such as glucose starvation (sustained induction of the CUP1 gene;
FF transient induction by heat) [2];
FF binds simultaneously with URSF to SSA1 promoter, but displaces URSF upon heat shock;
FF activation domain is repressed under non-shock conditions;
FF N-terminal sequences lead to the transient increase in HSF activation, C-terminal sequences to sustained increase concomitant with phosphorylation, presumably by SNF1 protein kinase [3] [2];
XX
IN T00385 HSF1; yeast, Saccharomyces cerevisiae.
IN T01264 HST; yeast, Kluyveromyces lactis.
XX
MX M00167 F$HSF_02.
MX M00168 F$HSF_03.
MX M00169 F$HSF_04.
MX M00170 F$HSF_05.
XX
BS R01773 AS$HSF_01; Quality: 4.
BS R03465 AS$HSF_04; Quality: 6.
BS R03823 AS$HSF_06; Quality: 1.
BS R03824 AS$HSF_07; Quality: 1.
BS R00751 DROME$HSP70_01; Quality: 6; hsp70, G000109; fruit fly, Drosophila melanogaster.
BS R00754 DROME$HSP70_04; Quality: 6; hsp70, G000109; fruit fly, Drosophila melanogaster.
BS R00756 DROME$HSP70_06; Quality: 2; hsp70, G000109; fruit fly, Drosophila melanogaster.
BS R01043 Y$CUP1_04; Quality: 2; CUP1, G000902; yeast, Saccharomyces cerevisiae.
BS R03320 Y$CUP1_08; Quality: 2; CUP1, G000902; yeast, Saccharomyces cerevisiae.
BS R00771 Y$HSP70_01; Quality: 4; hsp70, G000934; yeast, Saccharomyces cerevisiae.
BS R00775 Y$HSP83_03; Quality: 2; hsp83, G000935; yeast, Saccharomyces cerevisiae.
BS R00776 Y$HSP83_04; Quality: 2; hsp83, G000935; yeast, Saccharomyces cerevisiae.
BS R03007 Y$SSA3_01; Quality: 4; SSA3, G000983; yeast, Saccharomyces cerevisiae.
BS R03862 Y$SSA4_01; Quality: 5; SSA4, G000984; yeast, Saccharomyces cerevisiae.
XX
DR EMBL: J03139.
DR EMBL: M22040.
DR EMBL: Z72596.
DR SWISSPROT: P10961.
DR PIR: A31592.
DR PIR: A31593.
XX
RN [1]; RE0005288.
RX PUBMED: 8284672.
RA Harrison C. J., Bohm A. A., Nelson H. C. M.
RT Crystal structure of the DNA binding domain of the heat shock transcription factor
RL Science 263:224-227 (1994).
RN [2]; RE0005290.
RX PUBMED: 7969152.
RA Tamai K. T., Liu X., Silar P., Sosinowski T., Thiele D. J.
RT Heat shock transcription factor activates yeast metallothionein gene expression in response to heat and glucose starvation via distinct signalling pathways
RL Mol. Cell. Biol. 14:8155-8165 (1994).
RN [3]; RE0005287.
RX PUBMED: 1545786.
RA Bonner J. J., Heyward S., Fackenthal D. L.
RT Temperature-dependent regulation of a heterologous transcriptional activation domain fused to yeast heat shock transcription factor
RL Mol. Cell. Biol. 12:1021-1030 (1992).
RN [4]; RE0000761.
RX PUBMED: 2065978.
RA Park H.-O., Craig E. A.
RT Transcriptional regulation of a yeast HSP70 gene by heat shock factor and an upstream repression site-binding factor
RL Genes Dev. 5:1299-1308 (1991).
RN [5]; RE0000252.
RX PUBMED: 2201452.
RA Sorger P. K.
RT Yeast heat shock factor contains separable transient and sustained response transcriptional activators
RL Cell 62:793-805 (1990).
RN [6]; RE0000253.
RX PUBMED: 2201453.
RA Nieto-Sotelo J., Wiederrecht G., Okuda A., Parker C. S.
RT The yeast heat shock transcription factor contains a transcription activation domain whose activity is repressed under nonshock conditions
RL Cell 62:807-817 (1990).
RN [7]; RE0000251.
RX PUBMED: 2686840.
RA Sorger P. K., Nelson H. C. M.
RT Trimerization of a yeast transcriptional activator via a coiled-coil motif
RL Cell 59:807-813 (1989).
RN [8]; RE0001288.
RX PUBMED: 2664467.
RA Park H.-O., Craig E. A.
RT Positive and Negative Regulation of Basal Expression of a Yeast HSP70 Gene
RL Mol. Cell. Biol. 9:2025-2033 (1989).
RN [9]; RE0000083.
RX PUBMED: 3044612.
RA Wiederrecht G., Seto D., Parker C.
RT Isolation of the Gene Encoding the S. cerevisiae Heat Shock Transcription Factor
RL Cell 54:841-853 (1988).
RN [10]; RE0000146.
RX PUBMED: 3044613.
RA Sorger P. K., Pelham H. R. B.
RT Yeast Heat Shock Protein Is an Essential DNA-Binding Protein That Exhibits Temperature-Dependent Phosphorylation
RL Cell 54:855-864 (1988).
RN [11]; RE0000082.
RX PUBMED: 3100052.
RA Wiederrecht G., Shuey D. J., Kibbe W. A., Parker C. S.
RT The Saccharomyces and Drosophila heat shock transcription factors are identical in size and DNA binding properties
RL Cell 48:507-515 (1987).
RN [12]; RE0000416.
RX PUBMED: 3319580.
RA Sorger P. K., Pelham H. R. B.
RT Purification and characterization of a heat-shock element binding protein from yeast
RL EMBO J. 6:3035-3041 (1987).
XX
//
This version of the TRANSFAC database is free for users from non-profit organizations only.
Users from commercial organizations have to license the TRANSFAC databases and accompanying programs.