Resultats i conclusions
Una forma de comprovar el funcionament d'un programa bioinformatic es testar-lo amb seqüències de les cuals coneixem les característiques experimentalment.
Nosaltres hem provat el correcte funcionament d'EXON_fishing executant dues seqüències de les quals en coneixem l'anotació real dels exons.
Observant els resultats comprovem que el programa prediu tots els exons d'ambdues seqüències.
1. Seqüència NM_006533
Anotació real del gen (experimental).
NM_006533 chr19 Utr 1 70 . + . "H.sapiens"
NM_006533 chr19 First 71 197 . + . "H.sapiens"
NM_006533 chr19 Internal 280 413 . + "H.sapiens"
NM_006533 chr19 Internal 1497 1607 . + "H.sapiens"
NM_006533 chr19 Terminal 1925 1948 . + "H.sapiens"
NM_006533 chr19 Utr 1949 2019 . + . "H.sapiens"
Exons predits per EXONfishing en la seqüència NM_006533.
En color taronja es mostren els exons reals que el programa ha predit.
Selecció dels llindars
- llindar de puntuació dels senyals de splicing: 1.
- llindar de puntuació global dels exons predits: 2.
NM_006533 exon_fishing internal 65 85 5.09 + 1 NM_006533 exon_fishing internal 65 85 5.96 + 0 NM_006533 exon_fishing internal 65 85 4.53 + 2 NM_006533 exon_fishing terminal 65 165 2.09 + 1 NM_006533 exon_fishing internal 65 197 13.55 + 0 NM_006533 exon_fishing internal 65 197 6.99 + 2 NM_006533 exon_fishing internal 65 413 12.25 + 0 NM_006533 exon_fishing internal 65 413 16.60 + 2 NM_006533 exon_fishing terminal 65 460 10.48 + 0 NM_006533 exon_fishing terminal 65 509 17.35 + 2 NM_006533 exon_fishing firstATG 71 85 2.63 + 0 NM_006533 exon_fishing firstATG 71 197 10.22 + 0 NM_006533 exon_fishing firstATG 71 413 8.92 + 0 NM_006533 exon_fishing _single_ 71 460 7.15 + 0 NM_006533 exon_fishing firstATG 149 197 6.68 + 0 NM_006533 exon_fishing firstATG 149 413 5.38 + 0 NM_006533 exon_fishing _single_ 149 460 3.61 + 0 NM_006533 exon_fishing firstATG 204 413 12.05 + 0 NM_006533 exon_fishing _single_ 204 509 12.80 + 0 NM_006533 exon_fishing terminal 280 339 3.77 + 0 NM_006533 exon_fishing internal 280 413 2.93 + 2 NM_006533 exon_fishing internal 280 413 15.99 + 1 NM_006533 exon_fishing terminal 280 509 16.74 + 1 NM_006533 exon_fishing firstATG 291 413 10.38 + 0 NM_006533 exon_fishing _single_ 291 509 11.14 + 0 NM_006533 exon_fishing internal 309 413 10.20 + 0 NM_006533 exon_fishing terminal 309 509 10.96 + 0 NM_006533 exon_fishing firstATG 315 413 7.57 + 0 NM_006533 exon_fishing _single_ 315 509 8.32 + 0 NM_006533 exon_fishing terminal 600 635 2.72 + 0 NM_006533 exon_fishing terminal 737 792 3.08 + 1 NM_006533 exon_fishing terminal 740 792 2.13 + 1 NM_006533 exon_fishing firstATG 789 830 4.25 + 0 NM_006533 exon_fishing firstATG 1140 1174 2.54 + 0 NM_006533 exon_fishing _single_ 1225 1311 2.78 + 0 NM_006533 exon_fishing terminal 1497 1578 8.92 + 2 NM_006533 exon_fishing internal 1497 1607 18.68 + 0 NM_006533 exon_fishing internal 1497 1705 22.17 + 0 NM_006533 exon_fishing terminal 1497 1787 18.57 + 0 NM_006533 exon_fishing terminal 1503 1578 5.13 + 2 NM_006533 exon_fishing internal 1503 1607 14.59 + 0 NM_006533 exon_fishing internal 1503 1705 18.07 + 0 NM_006533 exon_fishing terminal 1503 1787 14.48 + 0 NM_006533 exon_fishing firstATG 1617 1705 5.99 + 0 NM_006533 exon_fishing _single_ 1617 1787 2.40 + 0 NM_006533 exon_fishing firstATG 1664 1705 3.22 + 0 NM_006533 exon_fishing firstATG 1674 1705 4.37 + 0 NM_006533 exon_fishing terminal 1925 1948 7.23 + 0 NM_006533 exon_fishing terminal 1925 2018 9.31 + 2 NM_006533 exon_fishing terminal 1925 2019 8.59 + 1
1. Seqüència NM_005641
Anotació real del gen (experimental).
NM_005641 chr7 Utr 1 477 . + . "H.sapiens"
NM_005641 chr7 Utr 5089 5147 . + . "H.sapiens"
NM_005641 chr7 First 5148 5303 . + . "H.sapiens"
NM_005641 chr7 Internal 5403 5489 . + . "H.sapiens"
NM_005641 chr7 Internal 5588 5741 . + . "H.sapiens"
NM_005641 chr7 Internal 5952 6008 . + . "H.sapiens"
NM_005641 chr7 Internal 6440 6559 . + . "H.sapiens"
NM_005641 chr7 Internal 7104 7249 . + . "H.sapiens"
NM_005641 chr7 Internal 7358 7435 . + . "H.sapiens"
NM_005641 chr7 Internal 7528 7629 . + . "H.sapiens"
NM_005641 chr7 Internal 8036 8218 . + . "H.sapiens"
NM_005641 chr7 Internal 9083 9157 . + . "H.sapiens"
NM_005641 chr7 Internal 9284 9409 . + . "H.sapiens"
NM_005641 chr7 Internal 10817 10990 . + . "H.sapiens"
NM_005641 chr7 Internal 11234 11431 . + . "H.sapiens"
NM_005641 chr7 Terminal 11734 12111 . + . "H.sapiens"
NM_005641 chr7 Utr 12112 12278 . + . "H.sapiens"
Exons predits per EXONfishing en la seqüència NM_005641.
En color taronja es mostren els exons reals que el programa ha predit.
Selecció dels llindars
- llindar de puntuació dels senyals de splicing: -2.
- llindar de puntuació global dels exons predits: 4.
NM_005641 exon_fishing terminal 596 636 4.30 + 1 NM_005641 exon_fishing internal 596 839 6.93 + 0 NM_005641 exon_fishing firstATG 653 839 4.90 + 0 NM_005641 exon_fishing internal 704 839 6.45 + 0 NM_005641 exon_fishing internal 708 839 7.36 + 1 NM_005641 exon_fishing internal 728 809 4.38 + 0 NM_005641 exon_fishing internal 728 839 7.79 + 0 NM_005641 exon_fishing internal 764 839 4.46 + 0 NM_005641 exon_fishing internal 867 961 8.76 + 2 NM_005641 exon_fishing internal 867 961 11.21 + 0 NM_005641 exon_fishing terminal 867 965 6.53 + 0 NM_005641 exon_fishing internal 867 1148 10.47 + 2 NM_005641 exon_fishing internal 867 1199 7.26 + 2 NM_005641 exon_fishing internal 867 1223 10.02 + 2 NM_005641 exon_fishing terminal 867 1227 6.28 + 2 NM_005641 exon_fishing firstATG 883 961 5.35 + 0 NM_005641 exon_fishing firstATG 883 1148 7.06 + 0 NM_005641 exon_fishing firstATG 883 1223 6.61 + 0 NM_005641 exon_fishing internal 894 961 4.95 + 2 NM_005641 exon_fishing internal 894 961 5.67 + 1 NM_005641 exon_fishing internal 894 961 6.80 + 0 NM_005641 exon_fishing internal 894 1148 6.66 + 2 NM_005641 exon_fishing internal 894 1223 6.21 + 2 NM_005641 exon_fishing internal 946 961 4.05 + 0 NM_005641 exon_fishing internal 946 961 4.15 + 2 NM_005641 exon_fishing internal 946 961 4.97 + 1 NM_005641 exon_fishing internal 946 1148 5.76 + 0 NM_005641 exon_fishing internal 946 1223 5.31 + 0 NM_005641 exon_fishing internal 1009 1148 7.78 + 0 NM_005641 exon_fishing internal 1009 1148 4.44 + 1 NM_005641 exon_fishing internal 1009 1199 4.57 + 0 NM_005641 exon_fishing internal 1009 1223 7.33 + 0 NM_005641 exon_fishing internal 1033 1148 5.01 + 0 NM_005641 exon_fishing internal 1033 1148 4.40 + 1 NM_005641 exon_fishing internal 1033 1223 4.56 + 0 NM_005641 exon_fishing internal 1199 1352 4.06 + 0 NM_005641 exon_fishing internal 1224 1352 6.53 + 1 NM_005641 exon_fishing internal 1257 1352 7.35 + 2 NM_005641 exon_fishing internal 1257 1352 5.13 + 1 NM_005641 exon_fishing internal 1257 1439 6.77 + 2 NM_005641 exon_fishing internal 1257 1500 4.89 + 2 NM_005641 exon_fishing internal 1257 1532 7.40 + 2 NM_005641 exon_fishing terminal 1257 1533 6.19 + 2 NM_005641 exon_fishing internal 1315 1352 7.49 + 0 NM_005641 exon_fishing internal 1315 1352 7.15 + 2 NM_005641 exon_fishing terminal 1315 1427 4.77 + 1 NM_005641 exon_fishing internal 1315 1439 6.92 + 0 NM_005641 exon_fishing internal 1315 1500 5.04 + 0 NM_005641 exon_fishing internal 1315 1532 7.54 + 0 NM_005641 exon_fishing terminal 1315 1533 6.34 + 0 NM_005641 exon_fishing internal 1319 1352 6.52 + 1 NM_005641 exon_fishing internal 1319 1352 6.31 + 0 NM_005641 exon_fishing terminal 1319 1427 4.95 + 2 NM_005641 exon_fishing internal 1319 1439 5.95 + 1 NM_005641 exon_fishing internal 1319 1500 4.07 + 1 NM_005641 exon_fishing internal 1319 1532 6.57 + 1 NM_005641 exon_fishing terminal 1319 1533 5.37 + 1 NM_005641 exon_fishing internal 1339 1352 4.13 + 0 NM_005641 exon_fishing internal 1339 1532 4.18 + 0 NM_005641 exon_fishing firstATG 1728 1836 8.22 + 0 NM_005641 exon_fishing _single_ 1728 1853 5.03 + 0 NM_005641 exon_fishing firstATG 1731 1836 7.88 + 0 NM_005641 exon_fishing _single_ 1731 1853 4.69 + 0 NM_005641 exon_fishing internal 1740 1836 6.87 + 0 NM_005641 exon_fishing internal 1740 1854 5.07 + 2 NM_005641 exon_fishing internal 1820 1854 4.53 + 1 NM_005641 exon_fishing internal 2040 2105 5.90 + 2 NM_005641 exon_fishing internal 2040 2236 7.16 + 2 NM_005641 exon_fishing internal 2040 2327 5.23 + 2 NM_005641 exon_fishing firstATG 2103 2236 6.67 + 0 NM_005641 exon_fishing internal 2139 2236 5.22 + 1 NM_005641 exon_fishing internal 2139 2236 6.41 + 0 NM_005641 exon_fishing firstATG 2524 2585 4.52 + 0 NM_005641 exon_fishing firstATG 2588 2644 6.25 + 0 NM_005641 exon_fishing internal 2745 2795 4.31 + 2 NM_005641 exon_fishing internal 2745 2801 5.03 + 2 NM_005641 exon_fishing terminal 2745 2805 5.44 + 2 NM_005641 exon_fishing internal 2883 2936 4.26 + 1 NM_005641 exon_fishing internal 2883 2980 4.79 + 1 NM_005641 exon_fishing internal 2883 3029 5.60 + 1 NM_005641 exon_fishing internal 2883 3032 7.57 + 1 NM_005641 exon_fishing terminal 2883 3106 7.19 + 1 NM_005641 exon_fishing internal 2886 3029 4.59 + 1 NM_005641 exon_fishing internal 2886 3032 6.55 + 1 NM_005641 exon_fishing terminal 2886 3106 6.17 + 1 NM_005641 exon_fishing internal 2965 3032 4.59 + 2 NM_005641 exon_fishing terminal 2965 3106 4.21 + 2 NM_005641 exon_fishing firstATG 3315 3356 5.71 + 0 NM_005641 exon_fishing firstATG 3315 3369 4.28 + 0 NM_005641 exon_fishing firstATG 3318 3356 5.37 + 0 NM_005641 exon_fishing internal 3414 3469 4.18 + 2 NM_005641 exon_fishing internal 3414 3469 4.63 + 0 NM_005641 exon_fishing internal 3414 3511 5.65 + 2 NM_005641 exon_fishing internal 3464 3469 4.16 + 2 NM_005641 exon_fishing internal 3464 3511 5.00 + 1 NM_005641 exon_fishing internal 3488 3511 4.67 + 2 NM_005641 exon_fishing internal 3551 3644 5.52 + 2 NM_005641 exon_fishing internal 3554 3644 5.85 + 2 NM_005641 exon_fishing internal 3701 3778 5.35 + 1 NM_005641 exon_fishing internal 3701 3778 4.72 + 2 NM_005641 exon_fishing internal 3701 3804 5.26 + 1 NM_005641 exon_fishing internal 3705 3778 5.82 + 2 NM_005641 exon_fishing internal 3705 3778 4.15 + 0 NM_005641 exon_fishing internal 3705 3804 5.73 + 2 NM_005641 exon_fishing terminal 4512 4608 4.32 + 2 NM_005641 exon_fishing terminal 4512 4616 5.47 + 0 NM_005641 exon_fishing internal 4651 4720 5.69 + 0 NM_005641 exon_fishing internal 4651 4720 8.30 + 1 NM_005641 exon_fishing terminal 4651 4724 7.01 + 1 NM_005641 exon_fishing internal 4651 4762 4.46 + 0 NM_005641 exon_fishing internal 4751 4762 4.23 + 0 NM_005641 exon_fishing internal 4802 4855 5.04 + 1 NM_005641 exon_fishing internal 4802 4888 5.56 + 1 NM_005641 exon_fishing internal 4802 4902 4.65 + 1 NM_005641 exon_fishing internal 4805 4855 4.10 + 1 NM_005641 exon_fishing internal 4805 4888 4.61 + 1 NM_005641 exon_fishing internal 4884 4970 6.69 + 0 NM_005641 exon_fishing internal 4884 5207 16.34 + 0 NM_005641 exon_fishing internal 4884 5210 15.73 + 0 NM_005641 exon_fishing internal 4884 5273 26.30 + 0 NM_005641 exon_fishing internal 4884 5303 29.13 + 0 NM_005641 exon_fishing terminal 4884 5378 25.86 + 0 NM_005641 exon_fishing internal 4901 5207 13.39 + 2 NM_005641 exon_fishing internal 4901 5210 12.78 + 2 NM_005641 exon_fishing internal 4901 5273 23.34 + 2 NM_005641 exon_fishing internal 4901 5303 26.18 + 2 NM_005641 exon_fishing terminal 4901 5378 22.90 + 2 NM_005641 exon_fishing internal 4927 5207 10.56 + 1 NM_005641 exon_fishing internal 4927 5210 9.95 + 1 NM_005641 exon_fishing internal 4927 5273 20.51 + 1 NM_005641 exon_fishing internal 4927 5303 23.35 + 1 NM_005641 exon_fishing terminal 4927 5378 20.07 + 1 NM_005641 exon_fishing internal 4932 5207 10.11 + 0 NM_005641 exon_fishing internal 4932 5210 9.50 + 0 NM_005641 exon_fishing internal 4932 5273 20.07 + 0 NM_005641 exon_fishing internal 4932 5303 22.90 + 0 NM_005641 exon_fishing terminal 4932 5378 19.63 + 0 NM_005641 exon_fishing internal 4960 4970 5.99 + 2 NM_005641 exon_fishing internal 4960 4970 6.20 + 1 NM_005641 exon_fishing terminal 4960 5155 7.86 + 2 NM_005641 exon_fishing internal 4960 5207 15.85 + 1 NM_005641 exon_fishing internal 4960 5210 15.24 + 1 NM_005641 exon_fishing internal 4960 5273 25.80 + 1 NM_005641 exon_fishing internal 4960 5303 28.63 + 1 NM_005641 exon_fishing terminal 4960 5378 25.36 + 1 NM_005641 exon_fishing firstATG 4977 5207 10.10 + 0 NM_005641 exon_fishing firstATG 4977 5210 9.49 + 0 NM_005641 exon_fishing firstATG 4977 5273 20.06 + 0 NM_005641 exon_fishing firstATG 4977 5303 22.89 + 0 NM_005641 exon_fishing _single_ 4977 5378 19.62 + 0 NM_005641 exon_fishing terminal 5089 5155 6.21 + 2 NM_005641 exon_fishing internal 5089 5207 13.07 + 1 NM_005641 exon_fishing internal 5089 5210 12.46 + 1 NM_005641 exon_fishing internal 5089 5273 23.03 + 1 NM_005641 exon_fishing internal 5089 5303 25.86 + 1 NM_005641 exon_fishing terminal 5089 5378 22.59 + 1 NM_005641 exon_fishing terminal 5126 5155 4.30 + 0 NM_005641 exon_fishing internal 5126 5207 13.60 + 2 NM_005641 exon_fishing internal 5126 5210 12.99 + 2 NM_005641 exon_fishing internal 5126 5273 23.56 + 2 NM_005641 exon_fishing internal 5126 5303 26.39 + 2 NM_005641 exon_fishing terminal 5126 5378 23.12 + 2 NM_005641 exon_fishing firstATG 5148 5207 8.40 + 0 NM_005641 exon_fishing firstATG 5148 5210 7.79 + 0 NM_005641 exon_fishing firstATG 5148 5273 18.36 + 0 NM_005641 exon_fishing firstATG 5148 5303 21.19 + 0 NM_005641 exon_fishing _single_ 5148 5378 17.92 + 0 NM_005641 exon_fishing firstATG 5202 5273 11.14 + 0 NM_005641 exon_fishing firstATG 5202 5303 13.98 + 0 NM_005641 exon_fishing _single_ 5202 5378 10.70 + 0 NM_005641 exon_fishing firstATG 5223 5273 7.90 + 0 NM_005641 exon_fishing firstATG 5223 5303 10.73 + 0 NM_005641 exon_fishing _single_ 5223 5378 7.46 + 0 NM_005641 exon_fishing internal 5238 5303 6.77 + 0 NM_005641 exon_fishing internal 5403 5489 11.26 + 0 NM_005641 exon_fishing internal 5403 5517 12.37 + 0 NM_005641 exon_fishing internal 5403 5639 13.26 + 0 NM_005641 exon_fishing internal 5403 5677 10.30 + 0 NM_005641 exon_fishing terminal 5403 5687 10.28 + 0 NM_005641 exon_fishing firstATG 5418 5489 7.68 + 0 NM_005641 exon_fishing firstATG 5418 5517 8.79 + 0 NM_005641 exon_fishing firstATG 5418 5639 9.68 + 0 NM_005641 exon_fishing firstATG 5418 5677 6.72 + 0 NM_005641 exon_fishing _single_ 5418 5687 6.70 + 0 NM_005641 exon_fishing firstATG 5424 5489 7.45 + 0 NM_005641 exon_fishing firstATG 5424 5517 8.56 + 0 NM_005641 exon_fishing firstATG 5424 5639 9.46 + 0 NM_005641 exon_fishing firstATG 5424 5677 6.49 + 0 NM_005641 exon_fishing _single_ 5424 5687 6.47 + 0 NM_005641 exon_fishing internal 5537 5639 6.84 + 0 NM_005641 exon_fishing internal 5537 5677 7.26 + 0 NM_005641 exon_fishing internal 5537 5716 10.90 + 0 NM_005641 exon_fishing internal 5537 5741 16.08 + 0 NM_005641 exon_fishing internal 5537 5762 15.40 + 0 NM_005641 exon_fishing internal 5537 5792 20.78 + 0 NM_005641 exon_fishing internal 5537 5796 19.65 + 0 NM_005641 exon_fishing internal 5537 5800 19.20 + 0 NM_005641 exon_fishing terminal 5537 5800 18.25 + 0 NM_005641 exon_fishing internal 5541 5639 8.51 + 1 NM_005641 exon_fishing internal 5541 5677 8.94 + 1 NM_005641 exon_fishing internal 5541 5716 12.58 + 1 NM_005641 exon_fishing internal 5541 5741 17.76 + 1 NM_005641 exon_fishing internal 5541 5762 17.08 + 1 NM_005641 exon_fishing internal 5541 5792 22.46 + 1 NM_005641 exon_fishing internal 5541 5796 21.33 + 1 NM_005641 exon_fishing internal 5541 5800 20.88 + 1 NM_005641 exon_fishing terminal 5541 5800 19.93 + 1 NM_005641 exon_fishing internal 5588 5639 10.53 + 0 NM_005641 exon_fishing internal 5588 5639 5.55 + 2 NM_005641 exon_fishing internal 5588 5677 10.95 + 0 NM_005641 exon_fishing internal 5588 5716 14.59 + 0 NM_005641 exon_fishing internal 5588 5741 19.77 + 0 NM_005641 exon_fishing internal 5588 5762 19.09 + 0 NM_005641 exon_fishing internal 5588 5792 24.47 + 0 NM_005641 exon_fishing internal 5588 5796 23.34 + 0 NM_005641 exon_fishing internal 5588 5800 22.89 + 0 NM_005641 exon_fishing terminal 5588 5800 21.94 + 0 NM_005641 exon_fishing internal 5612 5639 5.80 + 0 NM_005641 exon_fishing internal 5612 5677 6.22 + 0 NM_005641 exon_fishing internal 5612 5716 9.86 + 0 NM_005641 exon_fishing internal 5612 5741 15.04 + 0 NM_005641 exon_fishing internal 5612 5762 14.36 + 0 NM_005641 exon_fishing internal 5612 5792 19.74 + 0 NM_005641 exon_fishing internal 5612 5796 18.61 + 0 NM_005641 exon_fishing internal 5612 5800 18.16 + 0 NM_005641 exon_fishing terminal 5612 5800 17.21 + 0 NM_005641 exon_fishing internal 5742 5792 5.43 + 1 NM_005641 exon_fishing internal 5742 5796 4.31 + 1 NM_005641 exon_fishing internal 5742 5847 9.04 + 2 NM_005641 exon_fishing internal 5742 5847 8.58 + 0 NM_005641 exon_fishing terminal 5742 5933 6.09 + 0 NM_005641 exon_fishing firstATG 5791 5847 5.63 + 0 NM_005641 exon_fishing firstATG 5891 6008 7.65 + 0 NM_005641 exon_fishing _single_ 5891 6076 5.34 + 0 NM_005641 exon_fishing internal 5952 6008 8.56 + 2 NM_005641 exon_fishing internal 5952 6008 11.70 + 1 NM_005641 exon_fishing terminal 5952 6076 9.38 + 1 NM_005641 exon_fishing terminal 6009 6200 5.56 + 0 NM_005641 exon_fishing _single_ 6018 6200 5.70 + 0 NM_005641 exon_fishing terminal 6078 6204 4.93 + 2 NM_005641 exon_fishing terminal 6082 6200 4.62 + 1 NM_005641 exon_fishing terminal 6082 6204 5.77 + 0 NM_005641 exon_fishing internal 6082 6246 4.20 + 2 NM_005641 exon_fishing internal 6339 6482 9.99 + 2 NM_005641 exon_fishing internal 6339 6529 18.74 + 2 NM_005641 exon_fishing internal 6339 6559 24.38 + 2 NM_005641 exon_fishing terminal 6339 6573 19.21 + 2 NM_005641 exon_fishing internal 6353 6482 7.38 + 1 NM_005641 exon_fishing internal 6353 6529 16.14 + 1 NM_005641 exon_fishing internal 6353 6559 21.78 + 1 NM_005641 exon_fishing terminal 6353 6573 16.61 + 1 NM_005641 exon_fishing firstATG 6355 6482 9.35 + 0 NM_005641 exon_fishing firstATG 6355 6529 18.10 + 0 NM_005641 exon_fishing firstATG 6355 6559 23.74 + 0 NM_005641 exon_fishing _single_ 6355 6573 18.58 + 0 NM_005641 exon_fishing firstATG 6400 6482 8.77 + 0 NM_005641 exon_fishing firstATG 6400 6529 17.53 + 0 NM_005641 exon_fishing firstATG 6400 6559 23.17 + 0 NM_005641 exon_fishing _single_ 6400 6573 18.00 + 0 NM_005641 exon_fishing terminal 6440 6481 5.94 + 0 NM_005641 exon_fishing internal 6440 6482 8.74 + 1 NM_005641 exon_fishing internal 6440 6529 17.50 + 1 NM_005641 exon_fishing internal 6440 6559 23.14 + 1 NM_005641 exon_fishing terminal 6440 6573 17.97 + 1 NM_005641 exon_fishing internal 6449 6529 11.68 + 1 NM_005641 exon_fishing internal 6449 6559 17.32 + 1 NM_005641 exon_fishing terminal 6449 6573 12.15 + 1 NM_005641 exon_fishing internal 6646 6683 5.11 + 2 NM_005641 exon_fishing internal 6650 6683 4.32 + 0 NM_005641 exon_fishing internal 6670 6683 4.97 + 2 NM_005641 exon_fishing internal 6719 6767 4.81 + 1 NM_005641 exon_fishing internal 6811 6904 4.51 + 2 NM_005641 exon_fishing internal 6814 6904 5.67 + 0 NM_005641 exon_fishing internal 6814 6904 5.21 + 2 NM_005641 exon_fishing internal 6840 6904 4.33 + 1 NM_005641 exon_fishing internal 6891 6955 5.81 + 0 NM_005641 exon_fishing internal 6891 7032 5.74 + 0 NM_005641 exon_fishing terminal 6891 7037 4.69 + 0 NM_005641 exon_fishing internal 6905 6955 4.53 + 2 NM_005641 exon_fishing internal 6905 7032 4.46 + 2 NM_005641 exon_fishing firstATG 6917 6955 5.67 + 0 NM_005641 exon_fishing firstATG 6917 7032 7.80 + 0 NM_005641 exon_fishing _single_ 6917 7054 6.87 + 0 NM_005641 exon_fishing firstATG 6930 6955 4.00 + 0 NM_005641 exon_fishing internal 7021 7249 6.30 + 0 NM_005641 exon_fishing terminal 7021 7281 4.26 + 0 NM_005641 exon_fishing internal 7082 7222 11.57 + 0 NM_005641 exon_fishing internal 7082 7249 4.95 + 1 NM_005641 exon_fishing internal 7082 7249 18.30 + 0 NM_005641 exon_fishing terminal 7082 7336 18.47 + 0 NM_005641 exon_fishing internal 7104 7222 12.26 + 1 NM_005641 exon_fishing internal 7104 7249 6.09 + 2 NM_005641 exon_fishing internal 7104 7249 18.99 + 1 NM_005641 exon_fishing terminal 7104 7281 4.06 + 2 NM_005641 exon_fishing terminal 7104 7336 19.16 + 1 NM_005641 exon_fishing internal 7358 7435 8.32 + 1 NM_005641 exon_fishing internal 7358 7435 13.63 + 0 NM_005641 exon_fishing internal 7358 7435 6.98 + 2 NM_005641 exon_fishing terminal 7358 7447 10.07 + 0 NM_005641 exon_fishing internal 7358 7482 8.26 + 1 NM_005641 exon_fishing internal 7358 7527 12.59 + 1 NM_005641 exon_fishing internal 7358 7539 10.18 + 1 NM_005641 exon_fishing internal 7358 7579 14.18 + 1 NM_005641 exon_fishing internal 7358 7584 15.19 + 1 NM_005641 exon_fishing internal 7358 7629 18.57 + 1 NM_005641 exon_fishing internal 7358 7690 20.68 + 1 NM_005641 exon_fishing internal 7358 7697 21.12 + 1 NM_005641 exon_fishing terminal 7358 7719 19.82 + 1 NM_005641 exon_fishing internal 7433 7527 4.96 + 1 NM_005641 exon_fishing internal 7433 7579 6.55 + 1 NM_005641 exon_fishing internal 7433 7584 7.56 + 1 NM_005641 exon_fishing internal 7433 7629 10.94 + 1 NM_005641 exon_fishing internal 7433 7690 13.05 + 1 NM_005641 exon_fishing internal 7433 7697 13.49 + 1 NM_005641 exon_fishing terminal 7433 7719 12.19 + 1 NM_005641 exon_fishing firstATG 7444 7527 5.41 + 0 NM_005641 exon_fishing firstATG 7444 7579 7.00 + 0 NM_005641 exon_fishing firstATG 7444 7584 8.01 + 0 NM_005641 exon_fishing firstATG 7444 7629 11.39 + 0 NM_005641 exon_fishing firstATG 7444 7690 13.50 + 0 NM_005641 exon_fishing firstATG 7444 7697 13.94 + 0 NM_005641 exon_fishing _single_ 7444 7719 12.64 + 0 NM_005641 exon_fishing internal 7463 7527 4.08 + 1 NM_005641 exon_fishing internal 7463 7579 5.67 + 1 NM_005641 exon_fishing internal 7463 7584 6.67 + 1 NM_005641 exon_fishing internal 7463 7629 10.05 + 1 NM_005641 exon_fishing internal 7463 7690 12.16 + 1 NM_005641 exon_fishing internal 7463 7697 12.61 + 1 NM_005641 exon_fishing terminal 7463 7719 11.30 + 1 NM_005641 exon_fishing internal 7510 7579 5.57 + 0 NM_005641 exon_fishing internal 7510 7584 6.58 + 0 NM_005641 exon_fishing internal 7510 7629 9.96 + 0 NM_005641 exon_fishing internal 7510 7690 12.07 + 0 NM_005641 exon_fishing internal 7510 7697 12.51 + 0 NM_005641 exon_fishing terminal 7510 7719 11.21 + 0 NM_005641 exon_fishing terminal 7528 7537 5.60 + 2 NM_005641 exon_fishing internal 7528 7539 5.24 + 0 NM_005641 exon_fishing internal 7528 7539 4.79 + 1 NM_005641 exon_fishing internal 7528 7579 9.24 + 0 NM_005641 exon_fishing internal 7528 7584 10.25 + 0 NM_005641 exon_fishing internal 7528 7629 13.63 + 0 NM_005641 exon_fishing internal 7528 7629 5.03 + 1 NM_005641 exon_fishing internal 7528 7690 15.74 + 0 NM_005641 exon_fishing internal 7528 7697 16.18 + 0 NM_005641 exon_fishing terminal 7528 7719 14.88 + 0 NM_005641 exon_fishing firstATG 7576 7690 5.54 + 0 NM_005641 exon_fishing firstATG 7576 7697 5.99 + 0 NM_005641 exon_fishing _single_ 7576 7719 4.68 + 0 NM_005641 exon_fishing firstATG 7582 7629 4.21 + 0 NM_005641 exon_fishing firstATG 7582 7690 6.32 + 0 NM_005641 exon_fishing firstATG 7582 7697 6.76 + 0 NM_005641 exon_fishing _single_ 7582 7719 5.46 + 0 NM_005641 exon_fishing firstATG 7597 7690 4.85 + 0 NM_005641 exon_fishing firstATG 7597 7697 5.29 + 0 NM_005641 exon_fishing internal 7622 7690 4.20 + 1 NM_005641 exon_fishing internal 7622 7697 4.64 + 1 NM_005641 exon_fishing internal 7660 7690 7.83 + 0 NM_005641 exon_fishing internal 7660 7690 7.37 + 1 NM_005641 exon_fishing internal 7660 7697 8.27 + 0 NM_005641 exon_fishing internal 7660 7697 8.51 + 1 NM_005641 exon_fishing terminal 7660 7719 6.97 + 0 NM_005641 exon_fishing internal 7660 7745 11.33 + 1 NM_005641 exon_fishing terminal 7660 7787 10.63 + 1 NM_005641 exon_fishing internal 7667 7745 4.82 + 2 NM_005641 exon_fishing terminal 7667 7787 4.12 + 2 NM_005641 exon_fishing internal 7688 7745 5.60 + 2 NM_005641 exon_fishing terminal 7688 7787 4.91 + 2 NM_005641 exon_fishing internal 7874 7963 6.94 + 1 NM_005641 exon_fishing terminal 7874 8043 7.64 + 1 NM_005641 exon_fishing firstATG 7891 7963 4.79 + 0 NM_005641 exon_fishing _single_ 7891 8043 5.50 + 0 NM_005641 exon_fishing _single_ 7921 8043 4.35 + 0 NM_005641 exon_fishing terminal 8036 8043 4.28 + 1 NM_005641 exon_fishing terminal 8036 8048 4.80 + 2 NM_005641 exon_fishing internal 8036 8071 10.21 + 0 NM_005641 exon_fishing internal 8036 8143 11.20 + 0 NM_005641 exon_fishing internal 8036 8218 24.03 + 0 NM_005641 exon_fishing internal 8036 8268 22.20 + 0 NM_005641 exon_fishing internal 8036 8323 26.65 + 0 NM_005641 exon_fishing terminal 8036 8335 25.52 + 0 NM_005641 exon_fishing internal 8055 8218 15.09 + 1 NM_005641 exon_fishing internal 8055 8268 13.25 + 1 NM_005641 exon_fishing internal 8055 8323 17.71 + 1 NM_005641 exon_fishing terminal 8055 8335 16.58 + 1 NM_005641 exon_fishing firstATG 8060 8218 15.82 + 0 NM_005641 exon_fishing firstATG 8060 8268 13.99 + 0 NM_005641 exon_fishing firstATG 8060 8323 18.45 + 0 NM_005641 exon_fishing _single_ 8060 8335 17.32 + 0 NM_005641 exon_fishing internal 8100 8218 13.17 + 1 NM_005641 exon_fishing firstATG 8100 8218 4.22 + 0 NM_005641 exon_fishing internal 8100 8268 11.34 + 1 NM_005641 exon_fishing internal 8100 8323 15.79 + 1 NM_005641 exon_fishing terminal 8100 8335 14.67 + 1 NM_005641 exon_fishing internal 8153 8218 9.00 + 0 NM_005641 exon_fishing internal 8153 8268 7.17 + 0 NM_005641 exon_fishing internal 8153 8323 11.62 + 0 NM_005641 exon_fishing terminal 8153 8335 10.50 + 0 NM_005641 exon_fishing terminal 8381 8432 4.97 + 2 NM_005641 exon_fishing terminal 8381 8484 4.40 + 1 NM_005641 exon_fishing terminal 8381 8524 6.14 + 0 NM_005641 exon_fishing internal 8574 8587 5.67 + 2 NM_005641 exon_fishing internal 8574 8587 4.70 + 0 NM_005641 exon_fishing internal 8574 8655 7.03 + 2 NM_005641 exon_fishing internal 8574 8655 7.01 + 1 NM_005641 exon_fishing terminal 8574 8659 6.79 + 1 NM_005641 exon_fishing terminal 8574 8709 4.80 + 2 NM_005641 exon_fishing internal 8764 8805 5.39 + 0 NM_005641 exon_fishing internal 8909 8950 4.30 + 1 NM_005641 exon_fishing internal 8909 8950 7.06 + 0 NM_005641 exon_fishing internal 8909 8952 4.12 + 0 NM_005641 exon_fishing internal 9062 9204 4.43 + 0 NM_005641 exon_fishing terminal 9083 9144 5.91 + 1 NM_005641 exon_fishing internal 9083 9157 9.40 + 0 NM_005641 exon_fishing internal 9083 9157 4.61 + 2 NM_005641 exon_fishing terminal 9083 9203 7.80 + 2 NM_005641 exon_fishing internal 9083 9204 10.91 + 0 NM_005641 exon_fishing terminal 9083 9211 8.07 + 0 NM_005641 exon_fishing terminal 9135 9203 4.68 + 0 NM_005641 exon_fishing internal 9135 9204 4.24 + 1 NM_005641 exon_fishing terminal 9284 9372 4.85 + 1 NM_005641 exon_fishing internal 9284 9409 22.43 + 0 NM_005641 exon_fishing terminal 9284 9445 21.91 + 0 NM_005641 exon_fishing internal 9317 9409 12.50 + 0 NM_005641 exon_fishing terminal 9317 9445 11.99 + 0 NM_005641 exon_fishing internal 9320 9409 11.21 + 0 NM_005641 exon_fishing terminal 9320 9445 10.69 + 0 NM_005641 exon_fishing internal 9550 9573 4.42 + 1 NM_005641 exon_fishing internal 9737 9746 4.09 + 2 NM_005641 exon_fishing internal 10036 10073 6.74 + 0 NM_005641 exon_fishing internal 10036 10073 4.51 + 1 NM_005641 exon_fishing firstATG 10036 10142 5.01 + 0 NM_005641 exon_fishing internal 10036 10142 8.74 + 0 NM_005641 exon_fishing internal 10036 10142 4.13 + 1 NM_005641 exon_fishing terminal 10036 10146 6.82 + 0 NM_005641 exon_fishing internal 10060 10142 5.18 + 0 NM_005641 exon_fishing internal 10060 10142 4.38 + 2 NM_005641 exon_fishing internal 10111 10142 4.58 + 0 NM_005641 exon_fishing internal 10111 10142 4.73 + 2 NM_005641 exon_fishing internal 10199 10252 6.23 + 1 NM_005641 exon_fishing terminal 10199 10338 7.03 + 1 NM_005641 exon_fishing internal 10202 10252 5.81 + 1 NM_005641 exon_fishing terminal 10202 10338 6.62 + 1 NM_005641 exon_fishing terminal 10253 10300 5.18 + 0 NM_005641 exon_fishing terminal 10253 10338 4.88 + 1 NM_005641 exon_fishing internal 10398 10458 5.53 + 0 NM_005641 exon_fishing internal 10444 10458 4.33 + 2 NM_005641 exon_fishing internal 10447 10458 4.43 + 0 NM_005641 exon_fishing terminal 10527 10668 4.06 + 2 NM_005641 exon_fishing internal 10626 10918 6.47 + 1 NM_005641 exon_fishing internal 10626 10960 12.70 + 1 NM_005641 exon_fishing internal 10626 10990 15.60 + 1 NM_005641 exon_fishing internal 10626 11002 15.02 + 1 NM_005641 exon_fishing internal 10626 11041 12.99 + 1 NM_005641 exon_fishing internal 10626 11081 13.89 + 1 NM_005641 exon_fishing terminal 10626 11089 13.12 + 1 NM_005641 exon_fishing internal 10684 10918 11.46 + 2 NM_005641 exon_fishing internal 10684 10960 17.68 + 2 NM_005641 exon_fishing internal 10684 10990 20.59 + 2 NM_005641 exon_fishing internal 10684 11002 20.01 + 2 NM_005641 exon_fishing internal 10684 11041 17.97 + 2 NM_005641 exon_fishing internal 10684 11081 18.87 + 2 NM_005641 exon_fishing terminal 10684 11089 18.11 + 2 NM_005641 exon_fishing firstATG 10694 10918 8.45 + 0 NM_005641 exon_fishing firstATG 10694 10960 14.68 + 0 NM_005641 exon_fishing firstATG 10694 10990 17.58 + 0 NM_005641 exon_fishing firstATG 10694 11002 17.00 + 0 NM_005641 exon_fishing firstATG 10694 11041 14.97 + 0 NM_005641 exon_fishing firstATG 10694 11081 15.87 + 0 NM_005641 exon_fishing _single_ 10694 11089 15.10 + 0 NM_005641 exon_fishing firstATG 10704 10918 8.69 + 0 NM_005641 exon_fishing firstATG 10704 10960 8.95 + 0 NM_005641 exon_fishing _single_ 10704 10979 7.25 + 0 NM_005641 exon_fishing terminal 10730 10860 6.23 + 1 NM_005641 exon_fishing internal 10730 10918 10.62 + 0 NM_005641 exon_fishing internal 10730 10918 10.62 + 2 NM_005641 exon_fishing internal 10730 10960 16.84 + 0 NM_005641 exon_fishing internal 10730 10960 10.89 + 2 NM_005641 exon_fishing terminal 10730 10979 9.18 + 2 NM_005641 exon_fishing internal 10730 10990 19.75 + 0 NM_005641 exon_fishing internal 10730 11002 19.17 + 0 NM_005641 exon_fishing internal 10730 11041 17.13 + 0 NM_005641 exon_fishing internal 10730 11081 18.04 + 0 NM_005641 exon_fishing terminal 10730 11089 17.27 + 0 NM_005641 exon_fishing internal 10742 10918 7.40 + 0 NM_005641 exon_fishing internal 10742 10918 7.71 + 2 NM_005641 exon_fishing internal 10742 10960 13.63 + 0 NM_005641 exon_fishing internal 10742 10960 7.97 + 2 NM_005641 exon_fishing terminal 10742 10979 6.27 + 2 NM_005641 exon_fishing internal 10742 10990 16.53 + 0 NM_005641 exon_fishing internal 10742 11002 15.95 + 0 NM_005641 exon_fishing internal 10742 11041 13.92 + 0 NM_005641 exon_fishing internal 10742 11081 14.82 + 0 NM_005641 exon_fishing terminal 10742 11089 14.05 + 0 NM_005641 exon_fishing terminal 10782 10860 4.89 + 2 NM_005641 exon_fishing internal 10782 10918 11.13 + 1 NM_005641 exon_fishing internal 10782 10918 10.45 + 0 NM_005641 exon_fishing internal 10782 10960 17.35 + 1 NM_005641 exon_fishing internal 10782 10960 10.71 + 0 NM_005641 exon_fishing terminal 10782 10979 9.01 + 0 NM_005641 exon_fishing internal 10782 10990 20.26 + 1 NM_005641 exon_fishing internal 10782 11002 19.68 + 1 NM_005641 exon_fishing internal 10782 11041 17.64 + 1 NM_005641 exon_fishing internal 10782 11081 18.54 + 1 NM_005641 exon_fishing terminal 10782 11089 17.78 + 1 NM_005641 exon_fishing terminal 10817 10860 7.07 + 1 NM_005641 exon_fishing internal 10817 10918 12.58 + 0 NM_005641 exon_fishing internal 10817 10918 10.45 + 2 NM_005641 exon_fishing internal 10817 10960 18.81 + 0 NM_005641 exon_fishing internal 10817 10960 10.72 + 2 NM_005641 exon_fishing terminal 10817 10979 9.01 + 2 NM_005641 exon_fishing internal 10817 10990 21.71 + 0 NM_005641 exon_fishing internal 10817 11002 21.14 + 0 NM_005641 exon_fishing internal 10817 11041 19.10 + 0 NM_005641 exon_fishing internal 10817 11081 20.00 + 0 NM_005641 exon_fishing terminal 10817 11089 19.24 + 0 NM_005641 exon_fishing internal 10844 10960 9.13 + 0 NM_005641 exon_fishing internal 10844 10990 12.03 + 0 NM_005641 exon_fishing internal 10844 11002 11.45 + 0 NM_005641 exon_fishing internal 10844 11041 9.42 + 0 NM_005641 exon_fishing internal 10844 11081 10.32 + 0 NM_005641 exon_fishing terminal 10844 11089 9.55 + 0 NM_005641 exon_fishing internal 10868 10960 7.98 + 0 NM_005641 exon_fishing internal 10868 10990 10.88 + 0 NM_005641 exon_fishing internal 10868 11002 10.31 + 0 NM_005641 exon_fishing internal 10868 11041 8.27 + 0 NM_005641 exon_fishing internal 10868 11081 9.17 + 0 NM_005641 exon_fishing terminal 10868 11089 8.41 + 0 NM_005641 exon_fishing internal 10919 10960 5.84 + 1 NM_005641 exon_fishing internal 10919 10960 12.67 + 0 NM_005641 exon_fishing internal 10919 10960 6.97 + 2 NM_005641 exon_fishing terminal 10919 10979 5.26 + 2 NM_005641 exon_fishing internal 10919 10990 7.62 + 1 NM_005641 exon_fishing internal 10919 10990 15.58 + 0 NM_005641 exon_fishing internal 10919 11002 6.33 + 1 NM_005641 exon_fishing internal 10919 11002 15.00 + 0 NM_005641 exon_fishing terminal 10919 11019 4.12 + 1 NM_005641 exon_fishing internal 10919 11041 12.96 + 0 NM_005641 exon_fishing internal 10919 11081 13.86 + 0 NM_005641 exon_fishing terminal 10919 11089 13.10 + 0 NM_005641 exon_fishing internal 10952 10990 5.53 + 0 NM_005641 exon_fishing internal 10952 11002 4.95 + 0 NM_005641 exon_fishing internal 11096 11131 4.66 + 0 NM_005641 exon_fishing terminal 11096 11194 4.34 + 0 NM_005641 exon_fishing internal 11181 11335 4.59 + 2 NM_005641 exon_fishing internal 11181 11392 4.69 + 2 NM_005641 exon_fishing internal 11181 11431 10.45 + 2 NM_005641 exon_fishing terminal 11181 11508 9.41 + 2 NM_005641 exon_fishing internal 11195 11431 9.27 + 1 NM_005641 exon_fishing terminal 11195 11508 8.23 + 1 NM_005641 exon_fishing firstATG 11198 11335 7.57 + 0 NM_005641 exon_fishing internal 11198 11335 9.83 + 0 NM_005641 exon_fishing firstATG 11198 11392 9.13 + 0 NM_005641 exon_fishing internal 11198 11392 11.38 + 0 NM_005641 exon_fishing internal 11198 11431 9.72 + 1 NM_005641 exon_fishing firstATG 11198 11431 14.56 + 0 NM_005641 exon_fishing internal 11198 11431 16.82 + 0 NM_005641 exon_fishing terminal 11198 11508 8.68 + 1 NM_005641 exon_fishing terminal 11198 11578 17.65 + 0 NM_005641 exon_fishing _single_ 11198 11578 15.40 + 0 NM_005641 exon_fishing terminal 11234 11249 5.86 + 2 NM_005641 exon_fishing internal 11234 11335 7.25 + 1 NM_005641 exon_fishing internal 11234 11335 12.81 + 0 NM_005641 exon_fishing internal 11234 11392 7.34 + 1 NM_005641 exon_fishing internal 11234 11392 14.37 + 0 NM_005641 exon_fishing internal 11234 11431 13.10 + 1 NM_005641 exon_fishing internal 11234 11431 19.81 + 0 NM_005641 exon_fishing terminal 11234 11508 12.06 + 1 NM_005641 exon_fishing terminal 11234 11578 20.64 + 0 NM_005641 exon_fishing terminal 11261 11297 4.23 + 2 NM_005641 exon_fishing internal 11261 11335 7.99 + 0 NM_005641 exon_fishing internal 11261 11392 9.55 + 0 NM_005641 exon_fishing internal 11261 11431 7.22 + 1 NM_005641 exon_fishing internal 11261 11431 14.98 + 0 NM_005641 exon_fishing terminal 11261 11508 6.18 + 1 NM_005641 exon_fishing terminal 11261 11578 15.81 + 0 NM_005641 exon_fishing internal 11270 11335 4.94 + 0 NM_005641 exon_fishing internal 11270 11392 6.50 + 0 NM_005641 exon_fishing internal 11270 11431 4.77 + 1 NM_005641 exon_fishing internal 11270 11431 11.94 + 0 NM_005641 exon_fishing terminal 11270 11578 12.77 + 0 NM_005641 exon_fishing internal 11300 11431 4.67 + 1 NM_005641 exon_fishing internal 11300 11431 8.52 + 0 NM_005641 exon_fishing terminal 11300 11578 9.35 + 0 NM_005641 exon_fishing internal 11330 11431 7.10 + 1 NM_005641 exon_fishing internal 11330 11431 9.31 + 0 NM_005641 exon_fishing terminal 11330 11508 6.06 + 1 NM_005641 exon_fishing terminal 11330 11578 10.14 + 0 NM_005641 exon_fishing internal 11366 11431 4.20 + 1 NM_005641 exon_fishing internal 11366 11431 5.43 + 0 NM_005641 exon_fishing terminal 11366 11578 6.26 + 0 NM_005641 exon_fishing internal 11387 11431 4.10 + 1 NM_005641 exon_fishing internal 11387 11431 4.00 + 0 NM_005641 exon_fishing terminal 11387 11578 4.83 + 0 NM_005641 exon_fishing firstATG 11396 11431 4.99 + 0 NM_005641 exon_fishing _single_ 11396 11578 5.82 + 0 NM_005641 exon_fishing internal 11413 11431 5.56 + 0 NM_005641 exon_fishing internal 11413 11431 7.93 + 2 NM_005641 exon_fishing internal 11413 11431 5.63 + 1 NM_005641 exon_fishing terminal 11413 11508 4.52 + 0 NM_005641 exon_fishing terminal 11413 11578 8.76 + 2 NM_005641 exon_fishing terminal 11413 11591 11.38 + 1 NM_005641 exon_fishing internal 11429 11431 4.12 + 0 NM_005641 exon_fishing terminal 11429 11578 4.95 + 0 NM_005641 exon_fishing terminal 11429 11591 9.12 + 2 NM_005641 exon_fishing terminal 11432 11508 4.80 + 1 NM_005641 exon_fishing terminal 11432 11578 6.54 + 0 NM_005641 exon_fishing terminal 11432 11591 11.71 + 2 NM_005641 exon_fishing internal 11611 12153 7.21 + 1 NM_005641 exon_fishing internal 11611 12167 7.99 + 1 NM_005641 exon_fishing internal 11611 12272 5.88 + 1 NM_005641 exon_fishing terminal 11611 12278 6.83 + 1 NM_005641 exon_fishing internal 11621 12153 10.41 + 2 NM_005641 exon_fishing internal 11621 12167 11.19 + 2 NM_005641 exon_fishing internal 11621 12272 9.08 + 2 NM_005641 exon_fishing terminal 11621 12278 10.03 + 2 NM_005641 exon_fishing internal 11652 11825 6.01 + 2 NM_005641 exon_fishing internal 11652 11891 9.00 + 2 NM_005641 exon_fishing terminal 11652 12111 18.45 + 2 NM_005641 exon_fishing internal 11652 12153 5.70 + 0 NM_005641 exon_fishing internal 11652 12167 6.48 + 0 NM_005641 exon_fishing internal 11652 12272 4.36 + 0 NM_005641 exon_fishing terminal 11652 12278 5.31 + 0 NM_005641 exon_fishing internal 11677 11825 5.55 + 0 NM_005641 exon_fishing internal 11677 11891 8.54 + 0 NM_005641 exon_fishing terminal 11677 12111 18.00 + 0 NM_005641 exon_fishing internal 11677 12153 7.71 + 1 NM_005641 exon_fishing internal 11677 12167 8.48 + 1 NM_005641 exon_fishing internal 11677 12272 6.37 + 1 NM_005641 exon_fishing terminal 11677 12278 7.32 + 1 NM_005641 exon_fishing internal 11734 11825 9.79 + 0 NM_005641 exon_fishing internal 11734 11891 12.78 + 0 NM_005641 exon_fishing internal 11734 11891 4.67 + 1 NM_005641 exon_fishing terminal 11734 12111 22.23 + 0 NM_005641 exon_fishing internal 11734 12153 9.70 + 2 NM_005641 exon_fishing internal 11734 12153 13.43 + 1 NM_005641 exon_fishing internal 11734 12167 9.82 + 2 NM_005641 exon_fishing internal 11734 12167 14.21 + 1 NM_005641 exon_fishing terminal 11734 12190 6.56 + 2 NM_005641 exon_fishing internal 11734 12272 12.10 + 1 NM_005641 exon_fishing terminal 11734 12278 13.05 + 1 NM_005641 exon_fishing internal 11748 11825 4.06 + 2 NM_005641 exon_fishing internal 11748 11891 7.04 + 2 NM_005641 exon_fishing terminal 11748 12111 16.50 + 2 NM_005641 exon_fishing internal 11748 12153 5.78 + 1 NM_005641 exon_fishing internal 11748 12153 8.08 + 0 NM_005641 exon_fishing internal 11748 12167 5.89 + 1 NM_005641 exon_fishing internal 11748 12167 8.86 + 0 NM_005641 exon_fishing internal 11748 12272 6.75 + 0 NM_005641 exon_fishing terminal 11748 12278 7.70 + 0 NM_005641 exon_fishing internal 11768 11891 5.79 + 1 NM_005641 exon_fishing terminal 11768 12111 15.25 + 1 NM_005641 exon_fishing internal 11768 12153 4.21 + 0 NM_005641 exon_fishing internal 11768 12153 6.36 + 2 NM_005641 exon_fishing internal 11768 12167 4.32 + 0 NM_005641 exon_fishing internal 11768 12167 7.13 + 2 NM_005641 exon_fishing internal 11768 12272 5.02 + 2 NM_005641 exon_fishing terminal 11768 12278 5.97 + 2 NM_005641 exon_fishing internal 11808 11891 5.08 + 2 NM_005641 exon_fishing terminal 11808 12111 14.54 + 2 NM_005641 exon_fishing internal 11808 12153 7.59 + 1 NM_005641 exon_fishing internal 11808 12153 7.25 + 0 NM_005641 exon_fishing internal 11808 12167 7.70 + 1 NM_005641 exon_fishing internal 11808 12167 8.02 + 0 NM_005641 exon_fishing terminal 11808 12190 4.45 + 1 NM_005641 exon_fishing internal 11808 12272 5.91 + 0 NM_005641 exon_fishing terminal 11808 12278 6.86 + 0 NM_005641 exon_fishing terminal 11815 12111 11.60 + 0 NM_005641 exon_fishing internal 11815 12153 5.95 + 2 NM_005641 exon_fishing internal 11815 12153 6.18 + 1 NM_005641 exon_fishing internal 11815 12167 6.07 + 2 NM_005641 exon_fishing internal 11815 12167 6.95 + 1 NM_005641 exon_fishing internal 11815 12272 4.84 + 1 NM_005641 exon_fishing terminal 11815 12278 5.79 + 1 NM_005641 exon_fishing terminal 11859 12111 9.91 + 2 NM_005641 exon_fishing internal 11859 12153 10.89 + 1 NM_005641 exon_fishing internal 11859 12153 9.81 + 0 NM_005641 exon_fishing internal 11859 12167 11.01 + 1 NM_005641 exon_fishing internal 11859 12167 10.59 + 0 NM_005641 exon_fishing terminal 11859 12190 7.75 + 1 NM_005641 exon_fishing internal 11859 12272 8.47 + 0 NM_005641 exon_fishing terminal 11859 12278 9.42 + 0 NM_005641 exon_fishing terminal 11921 12111 11.22 + 1 NM_005641 exon_fishing internal 11921 12153 11.55 + 0 NM_005641 exon_fishing internal 11921 12153 14.22 + 2 NM_005641 exon_fishing internal 11921 12167 11.66 + 0 NM_005641 exon_fishing internal 11921 12167 14.99 + 2 NM_005641 exon_fishing terminal 11921 12190 8.41 + 0 NM_005641 exon_fishing internal 11921 12272 12.88 + 2 NM_005641 exon_fishing terminal 11921 12278 13.83 + 2 NM_005641 exon_fishing terminal 11960 12111 8.73 + 1 NM_005641 exon_fishing internal 11960 12153 7.76 + 0 NM_005641 exon_fishing internal 11960 12153 13.98 + 2 NM_005641 exon_fishing internal 11960 12167 7.87 + 0 NM_005641 exon_fishing internal 11960 12167 14.76 + 2 NM_005641 exon_fishing terminal 11960 12190 4.62 + 0 NM_005641 exon_fishing internal 11960 12272 12.64 + 2 NM_005641 exon_fishing terminal 11960 12278 13.60 + 2 NM_005641 exon_fishing terminal 11994 12111 8.57 + 2 NM_005641 exon_fishing internal 11994 12153 8.85 + 1 NM_005641 exon_fishing internal 11994 12153 9.67 + 0 NM_005641 exon_fishing internal 11994 12167 8.96 + 1 NM_005641 exon_fishing internal 11994 12167 10.45 + 0 NM_005641 exon_fishing terminal 11994 12190 5.71 + 1 NM_005641 exon_fishing internal 11994 12272 8.33 + 0 NM_005641 exon_fishing terminal 11994 12278 9.29 + 0 NM_005641 exon_fishing terminal 12000 12111 4.18 + 2 NM_005641 exon_fishing internal 12000 12153 4.99 + 0 NM_005641 exon_fishing internal 12000 12167 4.02 + 1 NM_005641 exon_fishing internal 12000 12167 5.76 + 0 NM_005641 exon_fishing terminal 12000 12278 4.60 + 0 NM_005641 exon_fishing internal 12047 12153 4.64 + 2 NM_005641 exon_fishing internal 12047 12167 5.42 + 2 NM_005641 exon_fishing terminal 12047 12278 4.26 + 2 NM_005641 exon_fishing terminal 12053 12111 5.51 + 1 NM_005641 exon_fishing internal 12053 12153 5.14 + 0 NM_005641 exon_fishing internal 12053 12153 7.77 + 2 NM_005641 exon_fishing internal 12053 12167 5.25 + 0 NM_005641 exon_fishing internal 12053 12167 8.55 + 2 NM_005641 exon_fishing internal 12053 12272 6.44 + 2 NM_005641 exon_fishing terminal 12053 12278 7.39 + 2 NM_005641 exon_fishing internal 12100 12153 4.58 + 1 NM_005641 exon_fishing internal 12100 12167 5.36 + 1 NM_005641 exon_fishing terminal 12100 12278 4.20 + 1
Conclusions
Amb aquestes seqüències hem pogut comprovar que el programa EXON-fishing és capaç de predir correctament els diferents exons codificants de les seqüències problema a l'strand positiu.
Tot i així, cal dir que el nostre programa no és capaç de predir regions UTR ja que no hi està preparat.
A més a més podem observar que tot i que té una alta sensibilitat presenta una baixa especificitat: a part de les prediccions dels exons correctes, trobem moltes altres prediccions que no són reals.
Veiem que l'EXON-fishing, com altres programes de predicció d'exons, només dóna una predicció que requereix una verificació mitjançant experiments in vitro. El treball in silico permet obtenir aproximacions útils de la realitat biològica, per això cal seguir treballant en aquest camp.
