CLUSTAL W (1.82) multiple sequence alignment D.melanogaster -------------MSYAADVLNSAHLELHGGGDAELRRPFDPTAHDLDASFRLTRFADLK 47 D.erecta -FFASLTPQLUNKMSYAADVLNSAHLELHGGGDAELRRPFDPTAHDLDASFRLTRFADLK 59 D.pseudoscura --KETLLLULUHTMSYAADVLNAAHLELHGGGDAELRRPFDPTAHDLDASFRLTRFADLK 58 D.mojavensis -ITFIULDYFDHKMSYAADVLNAAHLELHGGGDAELRRPFDPTAHDLDASFRLTRFADLK 59 D.virilis TIWFIULDFWN-KMSYAADVLNAAHLELHGGGDAELRRPFDPTAHDLDASFRLTRFADLK 59 D.ananasae --LFVVDQIYQURMSYAADVLNSAHLELHGGGDAELRRPFDPTAHDLDASFRLTRFADLK 58 A.gambiae --------------DYQQDPM--VHLELTGNTGMVLRRPFDPTAHDLDASFRLTRFADLK 44 SPS2 --MAEASATGACGEAMAAAEGSSGPAGLTLGRSFSNYRPFEPQALGLSPSWRLTGFSGMK 58 D.simulans -IWNTRNIDRYUSAATSLENDILYSQLFIKPVRRNSRTMFQPEKHGLSPDFQLTKFTTHT 59 D.yakuba -PSEVLRISISVQVSSLAQNTIFSIQLFLKPVLRISRTMFQPEKHGLEPDFQLTKFTTHT 59 : *:* .*...::** *: . D.melanogaster GRGCKVPQDVLSKLVSALQQDYS----------AQDQEPQFLNVAI--------PRIGIG 89 D.erecta GRGCKVPQDVLSKLVSALQQDYS----------AQDQEPQFLNVAI--------PRIGIG 101 D.pseudoscura GRGCKVPQDVLSKLVSALQQDYS----------AQDQEPQFLNVAI--------PRIGIG 100 D.mojavensis GRGCKVPQEVLSKLVSALQQDYS----------AQDQEPQFLNVAI--------PRIGIG 101 D.virilis GRGCKVPQEVLSKLVSALQQDYS----------AQDQEPQFLNVAI--------PRIGIG 101 D.ananasae GRGCKVPQDVLSKLVSALQQDYS----------TQDQEPQFLNVAI--------PRIGIG 100 A.gambiae GRCCKVPKEVLEKLVSSLQQDY-----------MQDPDQPFMPMSS--------PRIGIG 85 SPS2 GUGCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIG 118 D.simulans GUSCKIPQKVLEKYLRGTEIENKNN--DGYLIGERUYYQQIWGLSU-----WEIIFSGSG 112 D.yakuba GUSCKIPQKVLEKYLRGTEIENKKN--DGYLIGERUYYLQILGLSU-----CEILISGSG 112 * **:*:..* * : . : :. * * D.melanogaster LDCSVIPLR-HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLL 148 D.erecta LDCSVIPLR-HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLL 160 D.pseudoscura LDCSVIPLR-HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLL 159 D.mojavensis LDCAVIPLR-HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLL 160 D.virilis LDCAVIPLR-HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLL 160 D.ananasae LDCSVIPLR-HGGLCLVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTDCDNMLMLL 159 A.gambiae LDCSVIPLR-HGGLCMVQTTDFFYPIVDDPYMMGKIACANVLSDLYAMGVTEVDNMLMLL 144 SPS2 MDSCVIPLR-HGGLSLVQTTDFFYPLVEDPYMMGRIACANVLSDLYAMGITECDNMLMLL 177 D.simulans MDCAVIPLKRHKDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIV 172 D.yakuba MDCAVIPLRRHTDYLLIQTVDFFYPMVNDPELLGRIALANVLSDVYAVGVTQFDTVEMIV 172 :*..****: * . ::**.*****:*:** ::*:** ******:**:*:*: *.: *:: D.melanogaster AVSTKMTEKERDVVIPLIMRGFKDSALEAG---TTVTGGQSVVNPWCTIGGVASTICQPN 205 D.erecta AVSTKMTEKERDVVIPLIMRGFKDSALEAG---TTVTGGQSVVNPWCTIGGVASTICQPN 217 D.pseudoscura AVSTKMTEKERDVVIPLIMRGFKDSALEAG---TTVTGGQSVVNPWCTIGGVASTICQPN 216 D.mojavensis AVSTKMTEKERDVVIPLIMRGFKDSALEAG---TTVTGGQSVVNPWCTIGGVASTICQPN 217 D.virilis AVSTKMTEKERDVVIPLIMRGFKDSALEAG---TTVTGGQSVVNPWCTIGGVASTICQPN 217 D.ananasae AVSTKMTEKERDVVIPLIMRGFKDSALEAG---TTVTGGQSVVNPWCTIGGVASTICQPN 216 A.gambiae AVSTKMIEKERDVVIPLIMRGFKDSALEAG---TSVTGGHSVVNPWCTIGGVATTICQQN 201 SPS2 SVSQSMSEEEREKVTPLMVKGFRDAAEEGG---TAVTGGQTVVNPWIIIGGVATVVCQPN 234 D.simulans STSTSFSEKERDVVIGLVMKGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSE 232 D.yakuba STSTSFSEKERDTVIALVMQGFQNSLKANGYRNTPLIIRQLKINPWCIIGGIATSVCRSE 232 :.* .: *:**: * *:::**::: * *.: : :*** ***:*: :*: : D.melanogaster EYIVPDNAVVGDVLVLTKPLGT-QVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMN 264 D.erecta EYIVPDNAVVGDVLVLTKPLGT-QVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMN 276 D.pseudoscura EYIVPDNAVVGDVLVLTKPLGT-QVAVNAHQWIDQAERWNRIKLVVSEEDVRKAYHRAMN 275 D.mojavensis EYIVPDNAVVGDVLVLTKPLGT-QVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMN 276 D.virilis EYIVPDNAVVGDVLVLTKPLGT-QVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMN 276 D.ananasae EYIVPDNAVVGDVLVLTKPLGT-QVAVNAHQWIDQPERWNRIKLVVSEEDVRKAYHRAMN 275 A.gambiae EFIVPDNAVVGDVLVLTKALGT-QVAVNAHQWLDQSERWNRIKLVVSEEDVRKAYHRAMD 260 SPS2 EFIMPDSAVVGDVLVLTKPLGT-QVAVNAHQWLDNPERWNKVKMVVSREEVELAYQEAMF 293 D.simulans EIILUVLAWDDIFUFIUNTMPIUTVERAARRCVGPHETFGWPNGDGCPPLAAUPDRKVUE 292 D.yakuba EIILUIKAYDDIFKFIKYILPFUTVERAAWRCFGPHETLRWTNGDGCPPLAAUQDGKVRQ 292 * *: * . . .: : * * : .. * : . . .. D.melanogaster SMARLN--------RVAARLMHKYNAHGATDITGFGLLGHAQTLAAHQKKDVSFVIHNLP 316 D.erecta SMSRLN--------RVAARLMHKYNAHGATDITGFGLLGHAQTLAAHQKKDVSFVIHNLP 328 D.pseudoscura SMSRLN--------RVAARLMHKYNAHGATDITGFGLLGHAQTLAAHQKKDVSFVIHNLP 327 D.mojavensis SMSRLN--------RVAARLMHKYNAHGATDITGFGLLGHAQTLAAHQKKDVSFVIHNLP 328 D.virilis SMSRLN--------RVAARLMHKYNAHGATDITGFGLLGHAQTLAAHQKKDVSFVIHNLP 328 D.ananasae SMSRLN--------RVAARLMHKYNAHGATDITGFGLLGHAQTLAAHQKKDVSFVIHNLP 327 A.gambiae SMSRLN--------RVAARLMHKYNAHGATDVTGFGLLGHAQTLASHQKNEVSFVIHNLP 312 SPS2 NMATLN--------RTAAGLMHTFNAHAATDITGFGILGHSQNLAKQQRNEVSFVIHNLP 345 D.simulans TAVRMQUUUYKGNFUNSCEVYDLFEQKWLVLPWYSLFLU-LNNYLFQPPSUCTNIKRTVP 351 D.yakuba TAVUMUUUUYKRDFUNCCEVYDLFKQKWUVFLWNSLYYFULISFFSRP----FNAQISSA 348 . : .. : . :: : . . : . . D.melanogaster VIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQ---EGYQAWII 373 D.erecta VIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQ---EGYQAWII 385 D.pseudoscura VIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQ---EGYQAWII 384 D.mojavensis VIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQ---EGYQAWII 385 D.virilis VIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQ---EGYQAWII 385 D.ananasae VIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQ---EGYQAWII 384 A.gambiae VIAKMAAVAKACGNMFQLLQGHSAETSGGLLICLPREQAAAYCKDIEKQ---EGCQAWII 369 SPS2 IIAKMAAVSKASG-RFGLLQGTSAETSGGLLICLPREQAARFCSEIKSSKYGEGHQAWIV 404 D.simulans LISPDLVYWVT-PIIUHNSKRKRSYSRSTSCPSS--KMYLNSAPWWAKAQSFVLADRWKP 408 D.yakuba LCHUHYRLWATGPRU----KSSRISTRKGLIPNQUVAHHQKCTKIQHLGGPKHKVSFWQI 404 : : : : . * D.melanogaster GIVE-KGNKTARIIDKPRVIEVPAKD------------------- 398 D.erecta GIVE-KGNKTARIIDKPRVIEVPAKDUVSIAUPVAIVYLKYRNI- 428 D.pseudoscura GIVE-KGNKTARIIDKPRVIEVPAKDUMFTFYLYMYGILTULPLS 428 D.mojavensis GIVE-KGNKTARIIDKPRVIEVPAKDUMFSFILITVIFLHANTU- 428 D.virilis GIVE-KGNKTARIIDKPRVIEVPAKDUMFSLYISTVIFKKLHTR- 428 D.ananasae GIVE-KGNKTARIIDKPRVIEVPAKDUIIKPRWLIGFPIRFLSLI 428 A.gambiae GIVE-KGSRTARIIDKPRVIEVPAKD------------------- 394 SPS2 GIVE-KGNRTARIIDKPRVIEVLPRGATAAVLAPDSSNASSEPSS 448 D.simulans LVVFUYAFLLMPLINFAATLKKLPTESRNPFKLDM---------- 443 D.yakuba GRNLWRSLNLPSRUCRRSILPGLURSNQRRAKIFSNWTR------ 443 . :