CLUSTAL W (1.82) multiple sequence alignment D.erecta ----------------------------KYLUAPYHLUNRTLIP-VFITITYFQLKN-LL 30 D.yakuba -------------------------QVGSKLUQUSSL-DWURUPURMLUAFHMYIHAUIL 34 D.ananasae --------------------------LUFLPFSASKFAPSURPPTAGNWDSSKPSUCAPV 34 D.simulans ---------------------------QLMCSSCEKLDDFGLDTIKWVQUHLISRACFNH 33 D.virilis-1 PUWPLUAUVEU--PUVIIFIU-FTLPLQCLKSAHAEFTVAECRELGFIKTQLMCSNCDKL 57 D.virilis+1 S-ISFKU-CEUSWP-NLCNINUIISTLQCLKRTHAEFTAAQCRELGFIKTQLLCSNCDKL 57 D.pseudoscura -----------------------STLFQCQQ-THAEFTAADCRELGFIKAQLMCSSCDKL 36 D.melanogaster ----------------MHKCAIFLLLALSCQQIQAELTAADCRALGFIKAQLMCSSCEKL 44 A.subalbatus --------------MNKFLLSLIPVLVIVFQHTKAEFTTKDCRELGFIESQLFCSSCDTL 46 A.aegypti --------------------------MFIFQHIKAEFTAKDCRDLGFIKSQLYCSSCGTL 34 A.gambiae ----------------MRLFAITCLLFSIVTVIGAEFSAEDCRELGLIKSQLFCSSCSSL 44 A.mellifera ----------------------MLLDDYLVNIVSTEFSADDCKSLGFNKANLL------- 31 15-kDa ----MAAGPSGCLVPAFGLRLLLATVLQAVSAFGAEFSSEACRELGFSS-NLLCSSCDLL 55 : D.erecta SSCLITGIWAYAHICIFMFTLYFYIDSKPFLYFRPQCKQCCTLDQQP-AAQRTYAKAILE 89 D.yakuba QUYLILMRLGU---ITYYLLLPIFIESKHWYYFRPQCKQCCTLDQQP-AAQRTYAKAILE 90 D.ananasae VISWMTLDWRRSSRSLYFYSFAQYL----YCVLRPHCKQCCTLDQQP-AAQRTYAKAILE 89 D.simulans SIYTHANCFNU--SQNLIRSDALFLLSR-FUYFRPQCKQCCTLDQQP-AAQRTYAKAILE 89 D.virilis-1 DDFGLEA-------IKUAMLLULLTILTNLSL-RPHCKQCCTQDQQP-AAQRTYAKAILE 108 D.virilis+1 DDFGLEA-------IKUVTDUYLFEQITIHAVDRPHCKQCCTQDQQP-AAQRTYAKAILE 109 D.pseudoscura DDFGLET-------LKUVVVVHVVLLLUHPPFHRTPCKQCCTLDQQP-AAQRTYAKAILE 88 D.melanogaster DDFGLDT-------------------------IKPQCKQCCTLDQQP-AAQRTYAKAILE 78 A.subalbatus GEYGLDE-------------------------LKDHCRECCQKDAESSGKLMVYPKAVLE 81 A.aegypti GEYGLDE-------------------------LKDHCRECCQKDVESTGKLMVYPKAVLE 69 A.gambiae SDYGLIE-------------------------LKEHCLECCQKDTEADSKLKVYPAAVLE 79 A.mellifera ---------------------------------------CSTYDDYDASGLKRYPRAVLE 52 15-kDa GQFNLLQ-------------------------LDPDCRGCCQEEAQF-ETKKLYAGAILE 89 *. : *. *:** D.erecta VCTCKFRAYPQIQAFIQSGRPAKFPNLQIKYVRGLDPVVKLLDASGKVQETLSITKWNTD 149 D.yakuba VCTCKFRAYPQIQAFIQSGRPAKFPNLQIKYVRGLDPVVKLLDASGKVQETLSITKWNTD 150 D.ananasae VCTCKFRAYPQIQAFIQSGRPSKFPNLQIKYVRGLDPVVKLLDASGKVQETLSITKWNTD 149 D.simulans VCTCKFRAYPQIQAFIQSGRPAKFPNLQIKYVRGLDPVVKLLDASGKVQETLSITKWNTD 149 D.virilis-1 VCTCKFRAYPQIQAFIQSGRPAKFPNLQIKYVRGLDPIVKLLDASGKVQETLSITKWNTD 168 D.virilis+1 VCTCKFRAYPQIQAFIQSGRPAKFPNLQIKYVRGLDPLVKLLDASGKVQETLSITKWNTD 169 D.pseudoscura VCTCKFRAYPQIQAFIQSGRPAKFPNLQIKYVRGLDPVVKLLDAAGKVQETLSITKWNTD 148 D.melanogaster VCTCKFRAYPQIQAFIQSGRPAKFPNLQIKYVRGLDPVVKLLDASGKVQETLSITKWNTD 138 A.subalbatus VCTCKFGVYPQIQAFIKSDRPQKFPNLTIKYVRGLDPIVKLMDESGNVKETLSITKWNTD 141 A.aegypti VCTCKFGAYPQIQAFIKSDRPQKFPNLTIKYVRGLDPIVKLMDEAGNVKETLSITKWNTD 129 A.gambiae VCTCKFGAYPQIQAFIKSDRPAKFPNLTIKYVRGLDPIVKLMDEQGNVKETLSINKWNTD 139 A.mellifera VCTCKFGAYPQIQAFIKSNRPNKYKNLQIKYVRGLDPIIKLFDADNKVEDILDIHKWDTD 112 15-kDa VCGUKLGRFPQVQAFVRSDKPKLFRGLQIKYVRGSDPVLKLLDDNGNIAEELSILKWNTD 149 ** *: :**:***::*.:* : .* ****** **::**:* .:: : *.* **:** D.erecta TVEEFFETHLAKDGV------------------------- 164 D.yakuba TVEEFFETHLAKDG-------------------------- 164 D.ananasae TVEEFFETHLAKEGG------------------------- 164 D.simulans TVEEFFETHLAKDGA------------------------- 164 D.virilis-1 TVEEFFETHLAKEG-------------------------- 182 D.virilis+1 TVEEFFETHLSKEG-------------------------- 183 D.pseudoscura TVEEFFETHLAKDGK------------------------- 163 D.melanogaster TVEEFFETHLAKDGAGKNSYSVVEDADGDDDEDYLRTNRI 178 A.subalbatus TVQEFFETRLTKVTDEDDYLLTNRV--------------- 166 A.aegypti TVQEFFETRLTKVADDNDYLLTNRVQSLHV---------- 159 A.gambiae TVQEFFETRLAKVEDD-DYLKTNRV--------------- 163 A.mellifera SVDEFLATHLSMD--------------------------- 125 15-kDa SVEEFLSEKLERI--------------------------- 162 :*:**: :*