Resultats i conclusions

RESULTATS

Resultats per NM_006533

Resultats per NM_005641

Altres resultats

Comparació de resultats

CONCLUSIONS

CONCLUSIONS

RESULTATS

El programa EXON-finding és, doncs, un programa que prediu els exons presents en una seqüència de nucleòtids, basant-se en matrius de puntuació prèviament creades en estudis de moltes seqüències.

Per testar el funcionament del programa hem utilitzat dues seqüències de DNA humanes amb anotació coneguda. Són NM_006533 i NM_005641.

Resultats per NM_006533

Aquesta seqüència consta de 2029 nucleòtids i codifica per quatre exons. L'anotació real de la seqüència es mostra a continuació en format GFF: 
NM_006533	chr19	Utr	1	70	.	+	.	"H.sapiens"
NM_006533	chr19	First	71	197	.	+	.	"H.sapiens"
NM_006533	chr19	Internal	280	413	.	+	.	"H.sapiens"
NM_006533	chr19	Internal	1497	1607	.	+	.	"H.sapiens"
NM_006533	chr19	Terminal	1925	1948	.	+	.	"H.sapiens"
NM_006533	chr19	Utr	1949	2019	.	+	.	"H.sapiens"

Ajustant els paràmetres del programa obtenim les següents prediccions:

Els paràmetres utilitzats són:

Llindar de puntuació de les senyals d'splicing: 1
Nombre d'emparellaments màxim per cada senyal: 2
Llindar de puntuació global de l'exó: 2
Longitud mínima de l'exó: 20 

NM_006533       EXON-finding    First           71      197     10.222  +       0
NM_006533       EXON-finding    First           71      413     8.924   +       0
NM_006533       EXON-finding    First           149     197     6.678   +       0
NM_006533       EXON-finding    First           149     413     5.380   +       0
NM_006533       EXON-finding    First           204     413     12.050  +       0
NM_006533       EXON-finding    First           291     413     10.384  +       0
NM_006533       EXON-finding    First           315     413     7.567   +       0
NM_006533       EXON-finding    First           789     830     4.250   +       0
NM_006533       EXON-finding    First           1140    1174    2.541   +       0
NM_006533       EXON-finding    First           1617    1705    5.992   +       0
NM_006533       EXON-finding    First           1664    1705    3.217   +       0
NM_006533       EXON-finding    First           1674    1705    4.373   +       0
NM_006533       EXON-finding    Internal        65      85      5.090   +       2
NM_006533       EXON-finding    Internal        65      85      5.957   +       0
NM_006533       EXON-finding    Internal        65      85      4.533   +       1
NM_006533       EXON-finding    Internal        65      197     13.553  +       0
NM_006533       EXON-finding    Internal        65      197     6.986   +       1
NM_006533       EXON-finding    Internal        280     413     2.927   +       1
NM_006533       EXON-finding    Internal        280     413     15.988  +       2 
NM_006533       EXON-finding    Internal        309     413     10.203  +       0
NM_006533       EXON-finding    Internal        1497    1607    18.685  +       0 
NM_006533       EXON-finding    Internal        1497    1705    22.166  +       0
NM_006533       EXON-finding    Internal        1503    1607    14.593  +       0
NM_006533       EXON-finding    Internal        1503    1705    18.074  +       0
NM_006533       EXON-finding    Terminal        65      165     2.089   +       2
NM_006533       EXON-finding    Terminal        309     509     10.957  +       0
NM_006533       EXON-finding    Terminal        600     635     2.717   +       0
NM_006533       EXON-finding    Terminal        740     792     2.132   +       2
NM_006533       EXON-finding    Terminal        1503    1578    5.134   +       1
NM_006533       EXON-finding    Terminal        1925    1948    7.227   +       0
NM_006533       EXON-finding    Terminal        1925    2020    8.592   +       2
NM_006533       EXON-finding    Terminal        1925    2020    9.312   +       1

Les prediccions que estan marcades de color negre als resultats en format GFF corresponen amb els gens reals de la seqüència. Com s'observa, en aquest cas EXON-finding és capaç de predir tots els gens reals de la seqüència amb uns paràmetres bastant restrictius.

Resultats per NM_005641

Aquesta seqüència consta de 12277 nucleòtids i codifica per catorze exons. L'anotació real de la seqüència es mostra a continuació en format GFF: 
NM_005641	chr7	Utr	1	477	.	+	.	"H.sapiens"
NM_005641	chr7	Utr	5089	5147	.	+	.	"H.sapiens"
NM_005641	chr7	First	5148	5303	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	5403	5489	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	5588	5741	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	5952	6008	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	6440	6559	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	7104	7249	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	7358	7435	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	7528	7629	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	8036	8218	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	9083	9157	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	9284	9409	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	10817	10990	.	+	.	"H.sapiens"
NM_005641	chr7	Internal	11234	11431	.	+	.	"H.sapiens"
NM_005641	chr7	Terminal	11734	12111	.	+	.	"H.sapiens"
NM_005641	chr7	Utr	12112	12278	.	+	.	"H.sapiens"

Ajustant els paràmetres del programa obtenim les següents prediccions:

Els paràmetres utilitzats són:

Llindar de puntuació de les senyals d'splicing: -2
Nombre d'emparellaments màxim per cada senyal: 14
Llindar de puntuació global de l'exó: 4
Longitud mínima de l'exó: 50 


NM_005641	EXON-finding	First		653	839	4.902	+	0
NM_005641	EXON-finding	First		883	961	5.355	+	0
NM_005641	EXON-finding	First		883	1148	7.062	+	0
NM_005641	EXON-finding	First		883	1223	6.611	+	0
NM_005641	EXON-finding	First		1728	1836	8.219	+	0
NM_005641	EXON-finding	First		1728	1854	6.719	+	0
NM_005641	EXON-finding	First		1731	1836	7.883	+	0
NM_005641	EXON-finding	First		1731	1854	6.383	+	0
NM_005641	EXON-finding	First		2103	2236	6.667	+	0
NM_005641	EXON-finding	First		2524	2585	4.519	+	0
NM_005641	EXON-finding	First		2588	2644	6.251	+	0
NM_005641	EXON-finding	First		3315	3369	4.276	+	0
NM_005641	EXON-finding	First		4977	5207	10.104	+	0
NM_005641	EXON-finding	First		4977	5210	9.493	+	0
NM_005641	EXON-finding	First		4977	5273	20.060	+	0
NM_005641	EXON-finding	First		4977	5303	22.893	+	0
NM_005641	EXON-finding	First		5148	5207	8.398	+	0
NM_005641	EXON-finding	First		5148	5210	7.788	+	0
NM_005641	EXON-finding	First		5148	5273	18.355	+	0
NM_005641	EXON-finding	First		5148	5303	21.187	+	0
NM_005641	EXON-finding	First		5202	5273	11.144	+	0
NM_005641	EXON-finding	First		5202	5303	13.976	+	0
NM_005641	EXON-finding	First		5223	5273	7.898	+	0
NM_005641	EXON-finding	First		5223	5303	10.730	+	0
NM_005641	EXON-finding	First		5418	5489	7.679	+	0
NM_005641	EXON-finding	First		5418	5517	8.790	+	0
NM_005641	EXON-finding	First		5418	5639	9.682	+	0
NM_005641	EXON-finding	First		5418	5677	6.717	+	0
NM_005641	EXON-finding	First		5424	5489	7.453	+	0
NM_005641	EXON-finding	First		5424	5517	8.564	+	0
NM_005641	EXON-finding	First		5424	5639	9.456	+	0
NM_005641	EXON-finding	First		5424	5677	6.491	+	0
NM_005641	EXON-finding	First		5791	5847	5.634	+	0
NM_005641	EXON-finding	First		5891	6008	7.649	+	0
NM_005641	EXON-finding	First		6355	6482	9.346	+	0
NM_005641	EXON-finding	First		6355	6529	18.100	+	0
NM_005641	EXON-finding	First		6355	6559	23.741	+	0
NM_005641	EXON-finding	First		6400	6482	8.774	+	0
NM_005641	EXON-finding	First		6400	6529	17.528	+	0
NM_005641	EXON-finding	First		6400	6559	23.169	+	0
NM_005641	EXON-finding	First		6917	7032	7.799	+	0
NM_005641	EXON-finding	First		7444	7527	5.414	+	0
NM_005641	EXON-finding	First		7444	7579	7.004	+	0
NM_005641	EXON-finding	First		7444	7584	8.009	+	0
NM_005641	EXON-finding	First		7444	7629	11.393	+	0
NM_005641	EXON-finding	First		7444	7690	13.501	+	0
NM_005641	EXON-finding	First		7444	7697	13.943	+	0
NM_005641	EXON-finding	First		7576	7690	5.544	+	0
NM_005641	EXON-finding	First		7576	7697	5.987	+	0
NM_005641	EXON-finding	First		7582	7690	6.316	+	0
NM_005641	EXON-finding	First		7582	7697	6.759	+	0
NM_005641	EXON-finding	First		7597	7690	4.846	+	0
NM_005641	EXON-finding	First		7597	7697	5.289	+	0
NM_005641	EXON-finding	First		7891	7963	4.793	+	0
NM_005641	EXON-finding	First		8060	8218	15.824	+	0
NM_005641	EXON-finding	First		8060	8268	13.993	+	0
NM_005641	EXON-finding	First		8060	8323	18.447	+	0
NM_005641	EXON-finding	First		8100	8218	4.221	+	0
NM_005641	EXON-finding	First		10036	10142	5.015	+	0
NM_005641	EXON-finding	First		10694	10918	8.452	+	0
NM_005641	EXON-finding	First		10694	10960	14.678	+	0
NM_005641	EXON-finding	First		10694	10990	17.581	+	0
NM_005641	EXON-finding	First		10694	11002	17.004	+	0
NM_005641	EXON-finding	First		10694	11041	14.968	+	0
NM_005641	EXON-finding	First		10694	11081	15.868	+	0
NM_005641	EXON-finding	First		10704	10918	8.689	+	0
NM_005641	EXON-finding	First		10704	10960	8.953	+	0
NM_005641	EXON-finding	First		11198	11335	7.571	+	0
NM_005641	EXON-finding	First		11198	11392	9.126	+	0
NM_005641	EXON-finding	First		11198	11431	14.564	+	0
NM_005641	EXON-finding	Internal	596	839	6.925	+	0
NM_005641	EXON-finding	Internal	704	839	6.452	+	0
NM_005641	EXON-finding	Internal	708	839	7.361	+	2
NM_005641	EXON-finding	Internal	728	809	4.376	+	0
NM_005641	EXON-finding	Internal	728	839	7.791	+	0
NM_005641	EXON-finding	Internal	764	839	4.461	+	0
NM_005641	EXON-finding	Internal	867	961	8.760	+	1
NM_005641	EXON-finding	Internal	867	961	11.212	+	0
NM_005641	EXON-finding	Internal	867	1148	10.468	+	1
NM_005641	EXON-finding	Internal	867	1199	7.262	+	1
NM_005641	EXON-finding	Internal	867	1223	10.016	+	1
NM_005641	EXON-finding	Internal	894	961	4.953	+	1
NM_005641	EXON-finding	Internal	894	961	5.667	+	2
NM_005641	EXON-finding	Internal	894	961	6.804	+	0
NM_005641	EXON-finding	Internal	894	1148	6.661	+	1
NM_005641	EXON-finding	Internal	894	1223	6.210	+	1
NM_005641	EXON-finding	Internal	946	1148	5.760	+	0
NM_005641	EXON-finding	Internal	946	1223	5.309	+	0
NM_005641	EXON-finding	Internal	1009	1148	7.781	+	0
NM_005641	EXON-finding	Internal	1009	1148	4.444	+	2
NM_005641	EXON-finding	Internal	1009	1199	4.575	+	0
NM_005641	EXON-finding	Internal	1009	1223	7.330	+	0
NM_005641	EXON-finding	Internal	1033	1148	5.011	+	0
NM_005641	EXON-finding	Internal	1033	1148	4.401	+	2
NM_005641	EXON-finding	Internal	1033	1223	4.560	+	0
NM_005641	EXON-finding	Internal	1199	1352	4.065	+	0
NM_005641	EXON-finding	Internal	1224	1352	6.526	+	2
NM_005641	EXON-finding	Internal	1257	1352	7.347	+	1
NM_005641	EXON-finding	Internal	1257	1352	5.126	+	2
NM_005641	EXON-finding	Internal	1257	1439	6.772	+	1
NM_005641	EXON-finding	Internal	1257	1500	4.893	+	1
NM_005641	EXON-finding	Internal	1257	1532	7.397	+	1
NM_005641	EXON-finding	Internal	1315	1439	6.920	+	0
NM_005641	EXON-finding	Internal	1315	1500	5.041	+	0
NM_005641	EXON-finding	Internal	1315	1532	7.545	+	0
NM_005641	EXON-finding	Internal	1319	1439	5.947	+	2
NM_005641	EXON-finding	Internal	1319	1500	4.068	+	2
NM_005641	EXON-finding	Internal	1319	1532	6.572	+	2
NM_005641	EXON-finding	Internal	1339	1532	4.177	+	0
NM_005641	EXON-finding	Internal	1740	1836	6.867	+	0
NM_005641	EXON-finding	Internal	1740	1854	5.067	+	1
NM_005641	EXON-finding	Internal	1740	1854	5.368	+	0
NM_005641	EXON-finding	Internal	2040	2105	5.902	+	1
NM_005641	EXON-finding	Internal	2040	2236	7.159	+	1
NM_005641	EXON-finding	Internal	2040	2327	5.227	+	1
NM_005641	EXON-finding	Internal	2139	2236	5.217	+	2
NM_005641	EXON-finding	Internal	2139	2236	6.410	+	0
NM_005641	EXON-finding	Internal	2745	2795	4.305	+	1
NM_005641	EXON-finding	Internal	2745	2801	5.028	+	1
NM_005641	EXON-finding	Internal	2883	2936	4.265	+	2
NM_005641	EXON-finding	Internal	2883	2980	4.792	+	2
NM_005641	EXON-finding	Internal	2883	3029	5.601	+	2
NM_005641	EXON-finding	Internal	2883	3032	7.569	+	2
NM_005641	EXON-finding	Internal	2886	3029	4.586	+	2
NM_005641	EXON-finding	Internal	2886	3032	6.554	+	2
NM_005641	EXON-finding	Internal	2965	3032	4.588	+	1
NM_005641	EXON-finding	Internal	3414	3469	4.178	+	1
NM_005641	EXON-finding	Internal	3414	3469	4.630	+	0
NM_005641	EXON-finding	Internal	3414	3511	5.652	+	1
NM_005641	EXON-finding	Internal	3551	3644	5.521	+	1
NM_005641	EXON-finding	Internal	3554	3644	5.849	+	1
NM_005641	EXON-finding	Internal	3701	3778	5.349	+	2
NM_005641	EXON-finding	Internal	3701	3778	4.719	+	1
NM_005641	EXON-finding	Internal	3701	3804	5.259	+	2
NM_005641	EXON-finding	Internal	3705	3778	5.817	+	1
NM_005641	EXON-finding	Internal	3705	3778	4.154	+	0
NM_005641	EXON-finding	Internal	3705	3804	5.726	+	1
NM_005641	EXON-finding	Internal	4651	4720	5.691	+	0
NM_005641	EXON-finding	Internal	4651	4720	8.301	+	2
NM_005641	EXON-finding	Internal	4651	4762	4.458	+	0
NM_005641	EXON-finding	Internal	4802	4855	5.044	+	2
NM_005641	EXON-finding	Internal	4802	4888	5.558	+	2
NM_005641	EXON-finding	Internal	4802	4902	4.653	+	2
NM_005641	EXON-finding	Internal	4805	4855	4.100	+	2
NM_005641	EXON-finding	Internal	4805	4888	4.613	+	2
NM_005641	EXON-finding	Internal	4884	4970	6.691	+	0
NM_005641	EXON-finding	Internal	4884	5207	16.341	+	0
NM_005641	EXON-finding	Internal	4884	5210	15.730	+	0
NM_005641	EXON-finding	Internal	4884	5273	26.298	+	0
NM_005641	EXON-finding	Internal	4884	5303	29.130	+	0
NM_005641	EXON-finding	Internal	4901	5207	13.388	+	1
NM_005641	EXON-finding	Internal	4901	5210	12.777	+	1
NM_005641	EXON-finding	Internal	4901	5273	23.345	+	1
NM_005641	EXON-finding	Internal	4901	5303	26.177	+	1
NM_005641	EXON-finding	Internal	4927	5207	10.557	+	2
NM_005641	EXON-finding	Internal	4927	5210	9.946	+	2
NM_005641	EXON-finding	Internal	4927	5273	20.513	+	2
NM_005641	EXON-finding	Internal	4927	5303	23.346	+	2
NM_005641	EXON-finding	Internal	4932	5207	10.113	+	0
NM_005641	EXON-finding	Internal	4932	5210	9.503	+	0
NM_005641	EXON-finding	Internal	4932	5273	20.070	+	0
NM_005641	EXON-finding	Internal	4932	5303	22.902	+	0
NM_005641	EXON-finding	Internal	4960	5207	15.846	+	2
NM_005641	EXON-finding	Internal	4960	5210	15.235	+	2
NM_005641	EXON-finding	Internal	4960	5273	25.803	+	2
NM_005641	EXON-finding	Internal	4960	5303	28.635	+	2
NM_005641	EXON-finding	Internal	5089	5207	13.069	+	2
NM_005641	EXON-finding	Internal	5089	5210	12.459	+	2
NM_005641	EXON-finding	Internal	5089	5273	23.026	+	2
NM_005641	EXON-finding	Internal	5089	5303	25.858	+	2
NM_005641	EXON-finding	Internal	5126	5207	13.599	+	1
NM_005641	EXON-finding	Internal	5126	5210	12.989	+	1
NM_005641	EXON-finding	Internal	5126	5273	23.556	+	1
NM_005641	EXON-finding	Internal	5126	5303	26.388	+	1
NM_005641	EXON-finding	Internal	5238	5303	6.770	+	0
NM_005641	EXON-finding	Internal	5403	5489	11.260	+	0
NM_005641	EXON-finding	Internal	5403	5517	12.371	+	0
NM_005641	EXON-finding	Internal	5403	5639	13.263	+	0
NM_005641	EXON-finding	Internal	5403	5677	10.298	+	0
NM_005641	EXON-finding	Internal	5537	5639	6.836	+	0
NM_005641	EXON-finding	Internal	5537	5677	7.263	+	0
NM_005641	EXON-finding	Internal	5537	5716	10.897	+	0
NM_005641	EXON-finding	Internal	5537	5741	16.083	+	0
NM_005641	EXON-finding	Internal	5537	5762	15.400	+	0
NM_005641	EXON-finding	Internal	5537	5792	20.778	+	0
NM_005641	EXON-finding	Internal	5537	5796	19.651	+	0
NM_005641	EXON-finding	Internal	5537	5800	19.200	+	0
NM_005641	EXON-finding	Internal	5537	5802	17.826	+	0
NM_005641	EXON-finding	Internal	5541	5639	8.515	+	2
NM_005641	EXON-finding	Internal	5541	5677	8.942	+	2
NM_005641	EXON-finding	Internal	5541	5716	12.576	+	2
NM_005641	EXON-finding	Internal	5541	5741	17.761	+	2
NM_005641	EXON-finding	Internal	5541	5762	17.079	+	2
NM_005641	EXON-finding	Internal	5541	5792	22.456	+	2
NM_005641	EXON-finding	Internal	5541	5796	21.330	+	2
NM_005641	EXON-finding	Internal	5541	5800	20.879	+	2
NM_005641	EXON-finding	Internal	5541	5802	19.505	+	2
NM_005641	EXON-finding	Internal	5588	5639	10.525	+	0
NM_005641	EXON-finding	Internal	5588	5639	5.553	+	1
NM_005641	EXON-finding	Internal	5588	5677	10.952	+	0
NM_005641	EXON-finding	Internal	5588	5716	14.586	+	0
NM_005641	EXON-finding	Internal	5588	5741	19.772	+	0
NM_005641	EXON-finding	Internal	5588	5762	19.090	+	0
NM_005641	EXON-finding	Internal	5588	5792	24.467	+	0
NM_005641	EXON-finding	Internal	5588	5796	23.341	+	0
NM_005641	EXON-finding	Internal	5588	5800	22.890	+	0
NM_005641	EXON-finding	Internal	5588	5802	21.516	+	0
NM_005641	EXON-finding	Internal	5612	5677	6.225	+	0
NM_005641	EXON-finding	Internal	5612	5716	9.859	+	0
NM_005641	EXON-finding	Internal	5612	5741	15.044	+	0
NM_005641	EXON-finding	Internal	5612	5762	14.362	+	0
NM_005641	EXON-finding	Internal	5612	5792	19.739	+	0
NM_005641	EXON-finding	Internal	5612	5796	18.613	+	0
NM_005641	EXON-finding	Internal	5612	5800	18.162	+	0
NM_005641	EXON-finding	Internal	5612	5802	16.788	+	0
NM_005641	EXON-finding	Internal	5742	5792	5.432	+	2
NM_005641	EXON-finding	Internal	5742	5796	4.305	+	2
NM_005641	EXON-finding	Internal	5742	5847	9.042	+	1
NM_005641	EXON-finding	Internal	5742	5847	8.576	+	0
NM_005641	EXON-finding	Internal	5952	6008	8.559	+	1
NM_005641	EXON-finding	Internal	5952	6008	11.697	+	2
NM_005641	EXON-finding	Internal	6082	6246	4.200	+	1
NM_005641	EXON-finding	Internal	6339	6482	9.986	+	1
NM_005641	EXON-finding	Internal	6339	6529	18.740	+	1
NM_005641	EXON-finding	Internal	6339	6559	24.381	+	1
NM_005641	EXON-finding	Internal	6353	6482	7.381	+	2
NM_005641	EXON-finding	Internal	6353	6529	16.135	+	2
NM_005641	EXON-finding	Internal	6353	6559	21.776	+	2
NM_005641	EXON-finding	Internal	6440	6529	17.496	+	2
NM_005641	EXON-finding	Internal	6440	6559	23.137	+	2
NM_005641	EXON-finding	Internal	6449	6529	11.676	+	2
NM_005641	EXON-finding	Internal	6449	6559	17.317	+	2
NM_005641	EXON-finding	Internal	6811	6904	4.506	+	1
NM_005641	EXON-finding	Internal	6814	6904	5.674	+	0
NM_005641	EXON-finding	Internal	6814	6904	5.209	+	1
NM_005641	EXON-finding	Internal	6840	6904	4.330	+	2
NM_005641	EXON-finding	Internal	6891	6955	5.815	+	0
NM_005641	EXON-finding	Internal	6891	7032	5.741	+	0
NM_005641	EXON-finding	Internal	6905	6955	4.534	+	1
NM_005641	EXON-finding	Internal	6905	7032	4.461	+	1
NM_005641	EXON-finding	Internal	7021	7249	6.302	+	0
NM_005641	EXON-finding	Internal	7082	7222	11.571	+	0
NM_005641	EXON-finding	Internal	7082	7249	4.954	+	2
NM_005641	EXON-finding	Internal	7082	7249	18.300	+	0
NM_005641	EXON-finding	Internal	7104	7222	12.259	+	2
NM_005641	EXON-finding	Internal	7104	7249	6.094	+	1
NM_005641	EXON-finding	Internal	7104	7249	18.988	+	2
NM_005641	EXON-finding	Internal	7358	7435	8.319	+	2
NM_005641	EXON-finding	Internal	7358	7435	13.627	+	0
NM_005641	EXON-finding	Internal	7358	7435	6.980	+	1
NM_005641	EXON-finding	Internal	7358	7482	8.258	+	2
NM_005641	EXON-finding	Internal	7358	7527	12.592	+	2
NM_005641	EXON-finding	Internal	7358	7539	10.179	+	2
NM_005641	EXON-finding	Internal	7358	7579	14.183	+	2
NM_005641	EXON-finding	Internal	7358	7584	15.187	+	2
NM_005641	EXON-finding	Internal	7358	7629	18.571	+	2
NM_005641	EXON-finding	Internal	7358	7690	20.679	+	2
NM_005641	EXON-finding	Internal	7358	7697	21.122	+	2
NM_005641	EXON-finding	Internal	7433	7527	4.960	+	2
NM_005641	EXON-finding	Internal	7433	7579	6.551	+	2
NM_005641	EXON-finding	Internal	7433	7584	7.556	+	2
NM_005641	EXON-finding	Internal	7433	7629	10.939	+	2
NM_005641	EXON-finding	Internal	7433	7690	13.047	+	2
NM_005641	EXON-finding	Internal	7433	7697	13.490	+	2
NM_005641	EXON-finding	Internal	7463	7527	4.075	+	2
NM_005641	EXON-finding	Internal	7463	7579	5.666	+	2
NM_005641	EXON-finding	Internal	7463	7584	6.671	+	2
NM_005641	EXON-finding	Internal	7463	7629	10.054	+	2
NM_005641	EXON-finding	Internal	7463	7690	12.162	+	2
NM_005641	EXON-finding	Internal	7463	7697	12.605	+	2
NM_005641	EXON-finding	Internal	7510	7579	5.571	+	0
NM_005641	EXON-finding	Internal	7510	7584	6.576	+	0
NM_005641	EXON-finding	Internal	7510	7629	9.959	+	0
NM_005641	EXON-finding	Internal	7510	7690	12.067	+	0
NM_005641	EXON-finding	Internal	7510	7697	12.510	+	0
NM_005641	EXON-finding	Internal	7528	7579	9.241	+	0
NM_005641	EXON-finding	Internal	7528	7584	10.246	+	0
NM_005641	EXON-finding	Internal	7528	7629	13.629	+	0
NM_005641	EXON-finding	Internal	7528	7629	5.030	+	2
NM_005641	EXON-finding	Internal	7528	7690	15.737	+	0
NM_005641	EXON-finding	Internal	7528	7697	16.180	+	0
NM_005641	EXON-finding	Internal	7622	7690	4.200	+	2
NM_005641	EXON-finding	Internal	7622	7697	4.643	+	2
NM_005641	EXON-finding	Internal	7660	7745	11.327	+	2
NM_005641	EXON-finding	Internal	7667	7745	4.816	+	1
NM_005641	EXON-finding	Internal	7688	7745	5.602	+	1
NM_005641	EXON-finding	Internal	7874	7963	6.938	+	2
NM_005641	EXON-finding	Internal	8036	8143	11.201	+	0
NM_005641	EXON-finding	Internal	8036	8218	24.026	+	0
NM_005641	EXON-finding	Internal	8036	8268	22.195	+	0
NM_005641	EXON-finding	Internal	8036	8323	26.648	+	0
NM_005641	EXON-finding	Internal	8055	8218	15.085	+	2
NM_005641	EXON-finding	Internal	8055	8268	13.255	+	2
NM_005641	EXON-finding	Internal	8055	8323	17.708	+	2
NM_005641	EXON-finding	Internal	8100	8218	13.169	+	2
NM_005641	EXON-finding	Internal	8100	8268	11.338	+	2
NM_005641	EXON-finding	Internal	8100	8323	15.792	+	2
NM_005641	EXON-finding	Internal	8153	8218	8.998	+	0
NM_005641	EXON-finding	Internal	8153	8268	7.167	+	0
NM_005641	EXON-finding	Internal	8153	8323	11.621	+	0
NM_005641	EXON-finding	Internal	8574	8655	7.031	+	1
NM_005641	EXON-finding	Internal	8574	8655	7.006	+	2
NM_005641	EXON-finding	Internal	9062	9204	4.425	+	0
NM_005641	EXON-finding	Internal	9083	9157	9.396	+	0
NM_005641	EXON-finding	Internal	9083	9157	4.608	+	1
NM_005641	EXON-finding	Internal	9083	9204	10.905	+	0
NM_005641	EXON-finding	Internal	9083	9204	6.278	+	1
NM_005641	EXON-finding	Internal	9135	9204	4.239	+	2
NM_005641	EXON-finding	Internal	9284	9409	22.428	+	0
NM_005641	EXON-finding	Internal	9317	9409	12.505	+	0
NM_005641	EXON-finding	Internal	9320	9409	11.213	+	0
NM_005641	EXON-finding	Internal	10036	10142	8.738	+	0
NM_005641	EXON-finding	Internal	10036	10142	4.129	+	2
NM_005641	EXON-finding	Internal	10060	10142	5.179	+	0
NM_005641	EXON-finding	Internal	10060	10142	4.381	+	1
NM_005641	EXON-finding	Internal	10199	10252	6.231	+	2
NM_005641	EXON-finding	Internal	10202	10252	5.812	+	2
NM_005641	EXON-finding	Internal	10398	10458	5.533	+	0
NM_005641	EXON-finding	Internal	10626	10918	6.472	+	2
NM_005641	EXON-finding	Internal	10626	10960	12.698	+	2
NM_005641	EXON-finding	Internal	10626	10990	15.602	+	2
NM_005641	EXON-finding	Internal	10626	11002	15.024	+	2
NM_005641	EXON-finding	Internal	10626	11041	12.989	+	2
NM_005641	EXON-finding	Internal	10626	11081	13.889	+	2
NM_005641	EXON-finding	Internal	10684	10918	11.457	+	1
NM_005641	EXON-finding	Internal	10684	10960	17.683	+	1
NM_005641	EXON-finding	Internal	10684	10990	20.586	+	1
NM_005641	EXON-finding	Internal	10684	11002	20.009	+	1
NM_005641	EXON-finding	Internal	10684	11041	17.973	+	1
NM_005641	EXON-finding	Internal	10684	11081	18.873	+	1
NM_005641	EXON-finding	Internal	10730	10918	10.619	+	0
NM_005641	EXON-finding	Internal	10730	10918	10.624	+	1
NM_005641	EXON-finding	Internal	10730	10960	16.845	+	0
NM_005641	EXON-finding	Internal	10730	10960	10.887	+	1
NM_005641	EXON-finding	Internal	10730	10990	19.748	+	0
NM_005641	EXON-finding	Internal	10730	11002	19.171	+	0
NM_005641	EXON-finding	Internal	10730	11041	17.135	+	0
NM_005641	EXON-finding	Internal	10730	11081	18.035	+	0
NM_005641	EXON-finding	Internal	10742	10918	7.400	+	0
NM_005641	EXON-finding	Internal	10742	10918	7.711	+	1
NM_005641	EXON-finding	Internal	10742	10960	13.627	+	0
NM_005641	EXON-finding	Internal	10742	10960	7.974	+	1
NM_005641	EXON-finding	Internal	10742	10990	16.530	+	0
NM_005641	EXON-finding	Internal	10742	11002	15.953	+	0
NM_005641	EXON-finding	Internal	10742	11041	13.917	+	0
NM_005641	EXON-finding	Internal	10742	11081	14.817	+	0
NM_005641	EXON-finding	Internal	10782	10918	11.126	+	2
NM_005641	EXON-finding	Internal	10782	10918	10.448	+	0
NM_005641	EXON-finding	Internal	10782	10960	17.352	+	2
NM_005641	EXON-finding	Internal	10782	10960	10.711	+	0
NM_005641	EXON-finding	Internal	10782	10990	20.255	+	2
NM_005641	EXON-finding	Internal	10782	11002	19.678	+	2
NM_005641	EXON-finding	Internal	10782	11041	17.642	+	2
NM_005641	EXON-finding	Internal	10782	11081	18.542	+	2
NM_005641	EXON-finding	Internal	10817	10918	12.584	+	0
NM_005641	EXON-finding	Internal	10817	10918	10.452	+	1
NM_005641	EXON-finding	Internal	10817	10960	18.810	+	0
NM_005641	EXON-finding	Internal	10817	10960	10.715	+	1
NM_005641	EXON-finding	Internal	10817	10990	21.714	+	0
NM_005641	EXON-finding	Internal	10817	11002	21.136	+	0
NM_005641	EXON-finding	Internal	10817	11041	19.101	+	0
NM_005641	EXON-finding	Internal	10817	11081	20.001	+	0
NM_005641	EXON-finding	Internal	10844	10960	9.126	+	0
NM_005641	EXON-finding	Internal	10844	10990	12.029	+	0
NM_005641	EXON-finding	Internal	10844	11002	11.452	+	0
NM_005641	EXON-finding	Internal	10844	11041	9.416	+	0
NM_005641	EXON-finding	Internal	10844	11081	10.316	+	0
NM_005641	EXON-finding	Internal	10868	10960	7.981	+	0
NM_005641	EXON-finding	Internal	10868	10990	10.885	+	0
NM_005641	EXON-finding	Internal	10868	11002	10.307	+	0
NM_005641	EXON-finding	Internal	10868	11041	8.272	+	0
NM_005641	EXON-finding	Internal	10868	11081	9.172	+	0
NM_005641	EXON-finding	Internal	10919	10990	7.618	+	2
NM_005641	EXON-finding	Internal	10919	10990	15.578	+	0
NM_005641	EXON-finding	Internal	10919	11002	6.335	+	2
NM_005641	EXON-finding	Internal	10919	11002	15.000	+	0
NM_005641	EXON-finding	Internal	10919	11041	12.964	+	0
NM_005641	EXON-finding	Internal	10919	11081	13.864	+	0
NM_005641	EXON-finding	Internal	10952	11002	4.950	+	0
NM_005641	EXON-finding	Internal	11181	11335	4.590	+	1
NM_005641	EXON-finding	Internal	11181	11392	4.689	+	1
NM_005641	EXON-finding	Internal	11181	11431	10.446	+	1
NM_005641	EXON-finding	Internal	11195	11431	9.274	+	2
NM_005641	EXON-finding	Internal	11198	11335	9.829	+	0
NM_005641	EXON-finding	Internal	11198	11392	11.384	+	0
NM_005641	EXON-finding	Internal	11198	11431	9.720	+	2
NM_005641	EXON-finding	Internal	11198	11431	16.822	+	0
NM_005641	EXON-finding	Internal	11234	11335	7.245	+	2
NM_005641	EXON-finding	Internal	11234	11335	12.811	+	0
NM_005641	EXON-finding	Internal	11234	11392	7.344	+	2
NM_005641	EXON-finding	Internal	11234	11392	14.367	+	0
NM_005641	EXON-finding	Internal	11234	11431	13.100	+	2
NM_005641	EXON-finding	Internal	11234	11431	19.805	+	0
NM_005641	EXON-finding	Internal	11261	11335	7.990	+	0
NM_005641	EXON-finding	Internal	11261	11392	9.545	+	0
NM_005641	EXON-finding	Internal	11261	11431	7.218	+	2
NM_005641	EXON-finding	Internal	11261	11431	14.984	+	0
NM_005641	EXON-finding	Internal	11270	11335	4.942	+	0
NM_005641	EXON-finding	Internal	11270	11392	6.498	+	0
NM_005641	EXON-finding	Internal	11270	11431	4.773	+	2
NM_005641	EXON-finding	Internal	11270	11431	11.936	+	0
NM_005641	EXON-finding	Internal	11300	11431	4.669	+	2
NM_005641	EXON-finding	Internal	11300	11431	8.524	+	0
NM_005641	EXON-finding	Internal	11330	11431	7.105	+	2
NM_005641	EXON-finding	Internal	11330	11431	9.309	+	0
NM_005641	EXON-finding	Internal	11366	11431	4.196	+	2
NM_005641	EXON-finding	Internal	11366	11431	5.430	+	0
NM_005641	EXON-finding	Internal	11611	12153	7.214	+	2
NM_005641	EXON-finding	Internal	11611	12167	7.992	+	2
NM_005641	EXON-finding	Internal	11621	12153	10.413	+	1
NM_005641	EXON-finding	Internal	11621	12167	11.190	+	1
NM_005641	EXON-finding	Internal	11652	11825	6.014	+	1
NM_005641	EXON-finding	Internal	11652	11891	8.999	+	1
NM_005641	EXON-finding	Internal	11652	12153	5.698	+	0
NM_005641	EXON-finding	Internal	11652	12167	6.475	+	0
NM_005641	EXON-finding	Internal	11677	11825	5.555	+	0
NM_005641	EXON-finding	Internal	11677	11891	8.540	+	0
NM_005641	EXON-finding	Internal	11677	12153	7.707	+	2
NM_005641	EXON-finding	Internal	11677	12167	8.484	+	2
NM_005641	EXON-finding	Internal	11734	11825	9.793	+	0
NM_005641	EXON-finding	Internal	11734	11891	12.778	+	0
NM_005641	EXON-finding	Internal	11734	11891	4.666	+	2
NM_005641	EXON-finding	Internal	11734	12153	9.703	+	1
NM_005641	EXON-finding	Internal	11734	12153	13.432	+	2
NM_005641	EXON-finding	Internal	11734	12167	9.816	+	1
NM_005641	EXON-finding	Internal	11734	12167	14.209	+	2
NM_005641	EXON-finding	Internal	11748	11825	4.058	+	1
NM_005641	EXON-finding	Internal	11748	11891	7.043	+	1
NM_005641	EXON-finding	Internal	11748	12153	5.778	+	2
NM_005641	EXON-finding	Internal	11748	12153	8.083	+	0
NM_005641	EXON-finding	Internal	11748	12167	5.891	+	2
NM_005641	EXON-finding	Internal	11748	12167	8.860	+	0
NM_005641	EXON-finding	Internal	11768	11891	5.791	+	2
NM_005641	EXON-finding	Internal	11768	12153	4.211	+	0
NM_005641	EXON-finding	Internal	11768	12153	6.355	+	1
NM_005641	EXON-finding	Internal	11768	12167	4.324	+	0
NM_005641	EXON-finding	Internal	11768	12167	7.133	+	1
NM_005641	EXON-finding	Internal	11808	11891	5.085	+	1
NM_005641	EXON-finding	Internal	11808	12153	7.592	+	2
NM_005641	EXON-finding	Internal	11808	12153	7.247	+	0
NM_005641	EXON-finding	Internal	11808	12167	7.705	+	2
NM_005641	EXON-finding	Internal	11808	12167	8.025	+	0
NM_005641	EXON-finding	Internal	11815	12153	5.954	+	1
NM_005641	EXON-finding	Internal	11815	12153	6.177	+	2
NM_005641	EXON-finding	Internal	11815	12167	6.067	+	1
NM_005641	EXON-finding	Internal	11815	12167	6.955	+	2
NM_005641	EXON-finding	Internal	11859	12153	10.893	+	2
NM_005641	EXON-finding	Internal	11859	12153	9.809	+	0
NM_005641	EXON-finding	Internal	11859	12167	11.006	+	2
NM_005641	EXON-finding	Internal	11859	12167	10.586	+	0
NM_005641	EXON-finding	Internal	11921	12153	11.546	+	0
NM_005641	EXON-finding	Internal	11921	12153	14.217	+	1
NM_005641	EXON-finding	Internal	11921	12167	11.659	+	0
NM_005641	EXON-finding	Internal	11921	12167	14.994	+	1
NM_005641	EXON-finding	Internal	11960	12153	7.756	+	0
NM_005641	EXON-finding	Internal	11960	12153	13.979	+	1
NM_005641	EXON-finding	Internal	11960	12167	7.868	+	0
NM_005641	EXON-finding	Internal	11960	12167	14.757	+	1
NM_005641	EXON-finding	Internal	11994	12153	8.847	+	2
NM_005641	EXON-finding	Internal	11994	12153	9.669	+	0
NM_005641	EXON-finding	Internal	11994	12167	8.960	+	2
NM_005641	EXON-finding	Internal	11994	12167	10.447	+	0
NM_005641	EXON-finding	Internal	12000	12153	4.987	+	0
NM_005641	EXON-finding	Internal	12000	12167	4.020	+	2
NM_005641	EXON-finding	Internal	12000	12167	5.765	+	0
NM_005641	EXON-finding	Internal	12047	12153	4.641	+	1
NM_005641	EXON-finding	Internal	12047	12167	5.418	+	1
NM_005641	EXON-finding	Internal	12053	12153	5.139	+	0
NM_005641	EXON-finding	Internal	12053	12153	7.774	+	1
NM_005641	EXON-finding	Internal	12053	12167	5.252	+	0
NM_005641	EXON-finding	Internal	12053	12167	8.552	+	1
NM_005641	EXON-finding	Internal	12100	12153	4.582	+	2
NM_005641	EXON-finding	Internal	12100	12167	5.359	+	2
NM_005641	EXON-finding	Terminal	867	965	6.532	+	0
NM_005641	EXON-finding	Terminal	867	1227	6.284	+	1
NM_005641	EXON-finding	Terminal	1257	1533	6.193	+	1
NM_005641	EXON-finding	Terminal	1315	1427	4.765	+	2
NM_005641	EXON-finding	Terminal	1315	1533	6.340	+	0
NM_005641	EXON-finding	Terminal	1319	1427	4.951	+	1
NM_005641	EXON-finding	Terminal	1319	1533	5.367	+	2
NM_005641	EXON-finding	Terminal	2745	2805	5.438	+	1
NM_005641	EXON-finding	Terminal	2883	3106	7.190	+	2
NM_005641	EXON-finding	Terminal	2886	3106	6.174	+	2
NM_005641	EXON-finding	Terminal	2965	3106	4.209	+	1
NM_005641	EXON-finding	Terminal	4512	4608	4.323	+	1
NM_005641	EXON-finding	Terminal	4512	4616	5.466	+	0
NM_005641	EXON-finding	Terminal	4651	4724	7.012	+	2
NM_005641	EXON-finding	Terminal	4884	5378	25.858	+	0
NM_005641	EXON-finding	Terminal	4901	5378	22.905	+	1
NM_005641	EXON-finding	Terminal	4927	5378	20.073	+	2
NM_005641	EXON-finding	Terminal	4932	5378	19.630	+	0
NM_005641	EXON-finding	Terminal	4960	5155	7.863	+	1
NM_005641	EXON-finding	Terminal	4960	5378	25.363	+	2
NM_005641	EXON-finding	Terminal	5089	5155	6.209	+	1
NM_005641	EXON-finding	Terminal	5089	5378	22.586	+	2
NM_005641	EXON-finding	Terminal	5126	5378	23.116	+	1
NM_005641	EXON-finding	Terminal	5403	5687	10.278	+	0
NM_005641	EXON-finding	Terminal	5537	5800	18.246	+	0
NM_005641	EXON-finding	Terminal	5541	5800	19.925	+	2
NM_005641	EXON-finding	Terminal	5588	5800	21.936	+	0
NM_005641	EXON-finding	Terminal	5612	5800	17.208	+	0
NM_005641	EXON-finding	Terminal	5742	5933	6.086	+	0
NM_005641	EXON-finding	Terminal	5952	6076	9.384	+	2
NM_005641	EXON-finding	Terminal	6009	6200	5.562	+	0
NM_005641	EXON-finding	Terminal	6078	6204	4.932	+	1
NM_005641	EXON-finding	Terminal	6082	6200	4.620	+	2
NM_005641	EXON-finding	Terminal	6082	6204	5.773	+	0
NM_005641	EXON-finding	Terminal	6339	6573	19.215	+	1
NM_005641	EXON-finding	Terminal	6353	6573	16.610	+	2
NM_005641	EXON-finding	Terminal	6440	6573	17.971	+	2
NM_005641	EXON-finding	Terminal	6449	6573	12.151	+	2
NM_005641	EXON-finding	Terminal	6891	7037	4.693	+	0
NM_005641	EXON-finding	Terminal	7021	7281	4.264	+	0
NM_005641	EXON-finding	Terminal	7082	7336	18.467	+	0
NM_005641	EXON-finding	Terminal	7104	7281	4.056	+	1
NM_005641	EXON-finding	Terminal	7104	7336	19.155	+	2
NM_005641	EXON-finding	Terminal	7358	7447	10.071	+	0
NM_005641	EXON-finding	Terminal	7358	7719	19.818	+	2
NM_005641	EXON-finding	Terminal	7433	7719	12.186	+	2
NM_005641	EXON-finding	Terminal	7463	7719	11.301	+	2
NM_005641	EXON-finding	Terminal	7510	7719	11.206	+	0
NM_005641	EXON-finding	Terminal	7528	7719	14.876	+	0
NM_005641	EXON-finding	Terminal	7660	7719	6.967	+	0
NM_005641	EXON-finding	Terminal	7660	7787	10.631	+	2
NM_005641	EXON-finding	Terminal	7667	7787	4.120	+	1
NM_005641	EXON-finding	Terminal	7688	7787	4.907	+	1
NM_005641	EXON-finding	Terminal	7874	8043	7.640	+	2
NM_005641	EXON-finding	Terminal	8036	8335	25.524	+	0
NM_005641	EXON-finding	Terminal	8055	8335	16.584	+	2
NM_005641	EXON-finding	Terminal	8100	8335	14.667	+	2
NM_005641	EXON-finding	Terminal	8153	8335	10.496	+	0
NM_005641	EXON-finding	Terminal	8381	8432	4.974	+	1
NM_005641	EXON-finding	Terminal	8381	8484	4.403	+	2
NM_005641	EXON-finding	Terminal	8381	8524	6.143	+	0
NM_005641	EXON-finding	Terminal	8574	8659	6.787	+	2
NM_005641	EXON-finding	Terminal	8574	8709	4.797	+	1
NM_005641	EXON-finding	Terminal	9083	9144	5.912	+	2
NM_005641	EXON-finding	Terminal	9083	9203	7.802	+	1
NM_005641	EXON-finding	Terminal	9083	9211	8.074	+	0
NM_005641	EXON-finding	Terminal	9135	9203	4.681	+	0
NM_005641	EXON-finding	Terminal	9284	9372	4.853	+	2
NM_005641	EXON-finding	Terminal	9284	9445	21.910	+	0
NM_005641	EXON-finding	Terminal	9317	9445	11.987	+	0
NM_005641	EXON-finding	Terminal	9320	9445	10.695	+	0
NM_005641	EXON-finding	Terminal	10036	10146	6.819	+	0
NM_005641	EXON-finding	Terminal	10199	10338	7.034	+	2
NM_005641	EXON-finding	Terminal	10202	10338	6.615	+	2
NM_005641	EXON-finding	Terminal	10253	10338	4.882	+	2
NM_005641	EXON-finding	Terminal	10527	10668	4.060	+	1
NM_005641	EXON-finding	Terminal	10684	11089	18.109	+	1
NM_005641	EXON-finding	Terminal	10730	10860	6.227	+	2
NM_005641	EXON-finding	Terminal	10730	10979	9.183	+	1
NM_005641	EXON-finding	Terminal	10730	11089	17.271	+	0
NM_005641	EXON-finding	Terminal	10742	10979	6.270	+	1
NM_005641	EXON-finding	Terminal	10742	11089	14.053	+	0
NM_005641	EXON-finding	Terminal	10782	10860	4.887	+	1
NM_005641	EXON-finding	Terminal	10782	10979	9.008	+	0
NM_005641	EXON-finding	Terminal	10782	11089	17.778	+	2
NM_005641	EXON-finding	Terminal	10817	10979	9.011	+	1
NM_005641	EXON-finding	Terminal	10817	11089	19.237	+	0
NM_005641	EXON-finding	Terminal	10844	11089	9.552	+	0
NM_005641	EXON-finding	Terminal	10868	11089	8.408	+	0
NM_005641	EXON-finding	Terminal	10919	10979	5.264	+	1
NM_005641	EXON-finding	Terminal	10919	11019	4.120	+	2
NM_005641	EXON-finding	Terminal	10919	11089	13.101	+	0
NM_005641	EXON-finding	Terminal	11096	11194	4.342	+	0
NM_005641	EXON-finding	Terminal	11195	11508	8.233	+	2
NM_005641	EXON-finding	Terminal	11198	11508	8.679	+	2
NM_005641	EXON-finding	Terminal	11234	11508	12.060	+	2
NM_005641	EXON-finding	Terminal	11234	11578	20.636	+	0
NM_005641	EXON-finding	Terminal	11261	11508	6.177	+	2
NM_005641	EXON-finding	Terminal	11261	11578	15.815	+	0
NM_005641	EXON-finding	Terminal	11270	11578	12.767	+	0
NM_005641	EXON-finding	Terminal	11300	11578	9.355	+	0
NM_005641	EXON-finding	Terminal	11330	11508	6.064	+	2
NM_005641	EXON-finding	Terminal	11330	11578	10.140	+	0
NM_005641	EXON-finding	Terminal	11366	11578	6.261	+	0
NM_005641	EXON-finding	Terminal	11387	11578	4.833	+	0
NM_005641	EXON-finding	Terminal	11413	11508	4.520	+	0
NM_005641	EXON-finding	Terminal	11413	11578	8.759	+	1
NM_005641	EXON-finding	Terminal	11413	11591	11.379	+	2
NM_005641	EXON-finding	Terminal	11429	11578	4.954	+	0
NM_005641	EXON-finding	Terminal	11429	11591	9.122	+	1
NM_005641	EXON-finding	Terminal	11432	11508	4.804	+	2
NM_005641	EXON-finding	Terminal	11432	11578	6.541	+	0
NM_005641	EXON-finding	Terminal	11432	11591	11.711	+	1
NM_005641	EXON-finding	Terminal	11734	12111	22.233	+	0
NM_005641	EXON-finding	Terminal	11748	12111	16.499	+	1
NM_005641	EXON-finding	Terminal	11768	12111	15.246	+	2
NM_005641	EXON-finding	Terminal	11808	12111	14.540	+	1
NM_005641	EXON-finding	Terminal	11808	12190	4.452	+	2
NM_005641	EXON-finding	Terminal	11815	12111	11.603	+	0
NM_005641	EXON-finding	Terminal	11859	12111	9.906	+	1
NM_005641	EXON-finding	Terminal	11859	12190	7.754	+	2
NM_005641	EXON-finding	Terminal	11859	12279	9.425	+	0
NM_005641	EXON-finding	Terminal	11921	12111	11.217	+	2
NM_005641	EXON-finding	Terminal	11921	12190	8.406	+	0
NM_005641	EXON-finding	Terminal	11921	12279	13.833	+	1
NM_005641	EXON-finding	Terminal	11960	12111	8.728	+	2
NM_005641	EXON-finding	Terminal	11960	12190	4.616	+	0
NM_005641	EXON-finding	Terminal	11960	12279	13.595	+	1
NM_005641	EXON-finding	Terminal	11994	12111	8.566	+	1
NM_005641	EXON-finding	Terminal	11994	12190	5.707	+	2
NM_005641	EXON-finding	Terminal	11994	12279	9.285	+	0
NM_005641	EXON-finding	Terminal	12000	12111	4.184	+	1
NM_005641	EXON-finding	Terminal	12000	12279	4.603	+	0
NM_005641	EXON-finding	Terminal	12047	12279	4.256	+	1
NM_005641	EXON-finding	Terminal	12053	12111	5.513	+	2
NM_005641	EXON-finding	Terminal	12053	12279	7.390	+	1
NM_005641	EXON-finding	Terminal	12100	12279	4.198	+	2

Les prediccions que estan marcades de color negre als resultats en format GFF corresponen amb els exons reals de la seqüència. Com s'observa, en aquest cas EXON-finding també és capaç de predir tots els exons reals de la seqüència tot i que el nombre de prediccions generades és molt superior a l'anterior.

Altres resultats

En ambdós casos, observem que el programa ens retorna moltes prediccions inespecífiques, ja que cap dels paràmetres establerts ens permet fer un cribatge prou bo com per només obtenir aquelles que són certes. Tot i així, observem que la puntuació mitjana dels exons de la seqüència és lleugerament superior a la dels exons predits que no en formen part, tal i com es veu al gràfic que hi ha a continuació.

Tal com s'indica a la taula del gràfic, les columes 1 i 3 corresponen a les mitjanes de les puntuacions dels exons de la seqüència mentre que les columnes 2 i 4 són les mitjanes de les puntuacions de les altres prediccions en les mateixes condicions.

Comparació de resultats

El resultats obtinguts amb els paràmetres optimitzats pel nostre programa comparats amb els obtinguts amb el Geneid són força bons ja que obtenim menys prediccions (ja que l'usuari té l'opció de disminuir el nombre de prediccions obtinguts modificant els paràmetres), però el més important és que som capaços de predir els mateixos exons que són reals.

Cal dir que el nostre programa no és capaç de predir ni regions UTR ni gens single (aquells que només estan formats per un exó) ja que no hi està preparat. Això es pot veure en les imatges generades a partir de les prediccions del programa EXON-finding i de les del Geneid obtingudes mitjanç el programa gff2ps.

Els resultats del Geneid s'han obtingut fent la predicció només de gens First, Internal, i Terminal, que són els tipus de gens que prediu EXON-finding. En la part superior de la imatge es veuen les prediccions del programa EXON-finding per la seqüència i en la part inferior les realitzades pel Geneid.

  • Resultats per NM_006533.


  • Resultats per NM_005641.


    •

    CONCLUSIONS

    Amb aquestes seqüències hem pogut comprovar que el programa EXON-finding és capaç de predir correctament els diferents exons codificants de la seqüència en l'strand positiu.

    Tot i així, cal dir que encara que els programes de predicció d'exons (i de gens) poden ser una eina bioinformàtica molt útil quan estem treballant amb seqüències anònimes, quan treballem amb seqüències conegudes podem observar que tot i que tenen una alta sensibilitat presenten una baixa especificitat, ja que a més a més de les prediccions dels exons correctes, trobem moltes altres prediccions que no són reals.

    Poden existir diferents raons que ens puguin explicar aquest fet. L'ús de matrius de pesos per la determinació d'exons (mitjançant la cerca de senyals d'splicing) és el fet que majors problemes suposa, ja que una alta puntuació de les senyals no augmenta la veracitat d'aquestes, ja que no sempre les senyals que tenen una millor puntuació són les certes.

    Per tant, el que podem dir és que segurament les matrius de pesos no estan contemplant tots els factors determinats que utilitza la cèl·lula per fer l'splicing i per tant que les prediccions basades en aquest mètode no podran millorar fins que no es descobreixin i s'implementin aquests altres elements cis en les matrius.

    Així doncs, l'EXON-finding, com molts altres programes bioinformàtics en aquests moments, només dóna una predicció que en qualsevol cas s'haurà de verificar mitjançant experiments in vitro. El treball in silico permet obtenir aproximacions útils de la realitat biològica, per això cal seguir treballant en aquest camp.