RESULTADOS


SARDOR ALINEATOR beta v1.0
Ahora veremos una comparacion entre una alineacion de secuencias hecha por ClustalW vs la alineacion mediante SARDOR. Hemos seleccionado tres secuencias correspondientes al IFR (Interferon Regulatory Factor) de humano, raton y rata. Como comprobaremos la alineacion no es exacta aunque si muy parecida. La unica diferencia es que SARDOR aņade dos GAPS al principio de la alineacion mientras que CLUSTALW lo hace en medio de la secuencia; esto seguramente se debe a que no usamos tres parametros que si que estan presentes en el original (ver seccion articulo)

ALINEACION POR CLUSTALW



sp|P19182|IFR1_MOUSE      MPKNKKRNAPHRG--GGGGGGSGAATSAATAGGPHRTVQPFSDEHASIETMSHCSGYSDP
sp|P20695|IFR1_RAT        MPKNKKRNAPHRG--GGGGGGSGAATSAATTGGPHRTVQPFSDEDASIETMSHCSGYSDP
sp|O00458|IFR1_HUMAN      MPKNKKRNTPHRGSSAGGGGSGAAAATAATAGGQHRNVQPFSDEDASIETMSHCSGYSDP
                          ********:****  .****...**::***:** **.*******.***************

sp|P19182|IFR1_MOUSE      SSFAEDGPEVLDEEGTQEDLEYKLKGLIDLTLDKSAKTRQAALEGVKNALSSKVLYEFVL
sp|P20695|IFR1_RAT        SSFAEDGPEVLDEEGTQEDLEYKLKGLIDLTLDKSAKTRQAALEGVKNALSSKVLYEFVL
sp|O00458|IFR1_HUMAN      SSFAEDGPEVLDEEGTQEDLEYKRKGLIDLTLDKSAKTRQAALEGIKNALASKMLYEFIL
                          *********************** *********************:****:**:****:*

sp|P19182|IFR1_MOUSE      ERRMTLTDSIERCLKKGKSDEQRAAAAVASVLCIQLGPGFESEEILKTLGPILKKIICDG
sp|P20695|IFR1_RAT        ERRMTLTDSIERCLKKGKSDGQRAAAALASVLCIQLGPGLESEEILKTLGPILKKIICDG
sp|O00458|IFR1_HUMAN      ERRMTLTDSIERCLKKGKSDEQRAAAALASVLCIQLGPGIESEEILKTLGPILKKIICDG
                          ******************** ******:***********:********************

sp|P19182|IFR1_MOUSE      AASIQARQTCATCFGVCCFIATDDITELYSTLECFENIFTKSYLKEKDTNVTCSTPNTVL
sp|P20695|IFR1_RAT        TASIQARQTCATCFGVCCFIATDDITELYSTLECLEGIFTKSYLKEKDTNVPCSTPNTVL
sp|O00458|IFR1_HUMAN      SASMQARQTCATCFGVCCFIATDDITELYSTLECLENIFTKSYLKEKDTTVICSTPNTVL
                          :**:******************************:*.************.* ********

sp|P19182|IFR1_MOUSE      HISSLLAWTLLLTICPINEVKKKLELHFHKLPSLLSCDDVNMRIAAGESLALLFELARGM
sp|P20695|IFR1_RAT        HISSLLAWTLLLTICPISEVKKKLELHFHKLPSLLSCDDVNMRIAAGESLALLFELARGM
sp|O00458|IFR1_HUMAN      HISSLLAWTLLLTICPINEVKKKLEMHFHKLPSLLSCDDVNMRIAAGESLALLFELARGI
                          *****************.*******:*********************************:

sp|P19182|IFR1_MOUSE      ESDFFYEDMDSLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERDFPTETVKFGP
sp|P20695|IFR1_RAT        ESDFFYEDMDSLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERDFPTETVKFGP
sp|O00458|IFR1_HUMAN      ESDFFYEDMESLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERDFPTETIKFGP
                          *********:*********************************************:****

sp|P19182|IFR1_MOUSE      ERMYIDSWVKKHTYDTFKEVLGSGMQYHLQTNEFLRNVFELGPPVMLDAATLKTMKISRF
sp|P20695|IFR1_RAT        ERMYIDSWVKKHTYDTFKEALGSGMQYHLQTNEFLRNVFELGPPVMLDAATLKTMKIPRF
sp|O00458|IFR1_HUMAN      ERMYIDCWVKKHTYDTFKEVLGSGMQYHLQSNEFLRNVFELGPPVMLDAATLKTMKISRF
                          ******.************.**********:**************************.**

sp|P19182|IFR1_MOUSE      ERHLYNSAAFKARTKARSKCRDKRADVGEFL
sp|P20695|IFR1_RAT        ERHLYNSAAFKARTKARSKCRDKRADVGEFF
sp|O00458|IFR1_HUMAN      ERHLYNSAAFKARTKARSKCRDKRADVGEFF
                          ******************************:

ALINEACION POR CLUSTALW



sp|O00458|IFR1_HUMAN         MPKNKKRNTPHRGSSAGGGGSGAAAATAATAGGQHRNVQPFSDEDASIET
sp|P19182|IFR1_MOUSE         --MPKNKKRNAPHRGGGGGGGSGAATSAATAGGPHRTVQPFSDEHASIET
sp|P20695|IFR1_RAT I         --MPKNKKRNAPHRGGGGGGGSGAATSAATTGGPHRTVQPFSDEDASIET

sp|O00458|IFR1_HUMAN         MSHCSGYSDPSSFAEDGPEVLDEEGTQEDLEYKRKGLIDLTLDKSAKTRQ
sp|P19182|IFR1_MOUSE         MSHCSGYSDPSSFAEDGPEVLDEEGTQEDLEYKLKGLIDLTLDKSAKTRQ
sp|P20695|IFR1_RAT I         MSHCSGYSDPSSFAEDGPEVLDEEGTQEDLEYKLKGLIDLTLDKSAKTRQ

sp|O00458|IFR1_HUMAN         AALEGIKNALASKMLYEFILERRMTLTDSIERCLKKGKSDEQRAAAALAS
sp|P19182|IFR1_MOUSE         AALEGVKNALSSKVLYEFVLERRMTLTDSIERCLKKGKSDEQRAAAAVAS
sp|P20695|IFR1_RAT I         AALEGVKNALSSKVLYEFVLERRMTLTDSIERCLKKGKSDGQRAAAALAS

sp|O00458|IFR1_HUMAN         VLCIQLGPGIESEEILKTLGPILKKIICDGSASMQARQTCATCFGVCCFI
sp|P19182|IFR1_MOUSE         VLCIQLGPGFESEEILKTLGPILKKIICDGAASIQARQTCATCFGVCCFI
sp|P20695|IFR1_RAT I         VLCIQLGPGLESEEILKTLGPILKKIICDGTASIQARQTCATCFGVCCFI

sp|O00458|IFR1_HUMAN         ATDDITELYSTLECLENIFTKSYLKEKDTTVICSTPNTVLHISSLLAWTL
sp|P19182|IFR1_MOUSE         ATDDITELYSTLECFENIFTKSYLKEKDTNVTCSTPNTVLHISSLLAWTL
sp|P20695|IFR1_RAT I         ATDDITELYSTLECLEGIFTKSYLKEKDTNVPCSTPNTVLHISSLLAWTL

sp|O00458|IFR1_HUMAN         LLTICPINEVKKKLEMHFHKLPSLLSCDDVNMRIAAGESLALLFELARGI
sp|P19182|IFR1_MOUSE         LLTICPINEVKKKLELHFHKLPSLLSCDDVNMRIAAGESLALLFELARGM
sp|P20695|IFR1_RAT I         LLTICPISEVKKKLELHFHKLPSLLSCDDVNMRIAAGESLALLFELARGM

sp|O00458|IFR1_HUMAN         ESDFFYEDMESLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERD
sp|P19182|IFR1_MOUSE         ESDFFYEDMDSLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERD
sp|P20695|IFR1_RAT I         ESDFFYEDMDSLTQMLRALATDGNKHRAKVDKRKQRSVFRDVLRAVEERD

sp|O00458|IFR1_HUMAN         FPTETIKFGPERMYIDCWVKKHTYDTFKEVLGSGMQYHLQSNEFLRNVFE
sp|P19182|IFR1_MOUSE         FPTETVKFGPERMYIDSWVKKHTYDTFKEVLGSGMQYHLQTNEFLRNVFE
sp|P20695|IFR1_RAT I         FPTETVKFGPERMYIDSWVKKHTYDTFKEALGSGMQYHLQTNEFLRNVFE

sp|O00458|IFR1_HUMAN         LGPPVMLDAATLKTMKISRFERHLYNSAAFKARTKARSKCRDKRADVGEF
sp|P19182|IFR1_MOUSE         LGPPVMLDAATLKTMKISRFERHLYNSAAFKARTKARSKCRDKRADVGEF
sp|P20695|IFR1_RAT I         LGPPVMLDAATLKTMKIPRFERHLYNSAAFKARTKARSKCRDKRADVGEF

sp|O00458|IFR1_HUMAN         F
sp|P19182|IFR1_MOUSE         L
sp|P20695|IFR1_RAT I         F
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