Resultats en format txt de la seqüència sense emmascarar: GENSCANW output for sequence AC010727 GENSCAN 1.0 Date run: 28-Feb-102 Time: 12:01:09 Sequence AC010727 : 148061 bp : 42.55% C+G : Isochore 1 ( 0 - 43 C+G%) Parameter matrix: HumanIso.smat Predicted genes/exons: Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr.. ----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------ 1.01 Init + 9671 9757 87 1 0 56 30 149 0.024 4.59 1.02 Intr + 10031 10116 86 1 2 78 98 53 0.012 3.00 1.03 Intr + 23152 23251 100 1 1 111 68 64 0.050 5.89 1.04 Intr + 26798 26957 160 1 1 45 55 186 0.006 9.64 1.05 Term + 29924 30084 161 0 2 102 32 108 0.057 3.72 1.06 PlyA + 30327 30332 6 -1.95 2.05 PlyA - 30396 30391 6 1.05 2.04 Term - 30900 30827 74 1 2 65 38 53 0.200 -4.91 2.03 Intr - 31241 31069 173 1 2 122 70 117 0.562 11.96 2.02 Intr - 32642 32473 170 2 2 89 57 122 0.956 7.02 2.01 Init - 39413 39360 54 2 0 52 88 56 0.847 3.34 2.00 Prom - 39785 39746 40 -5.05 3.07 PlyA - 40441 40436 6 -0.45 3.06 Term - 41073 40984 90 0 0 101 42 113 0.229 4.74 3.05 Intr - 46079 46026 54 2 0 92 30 100 0.116 2.86 3.04 Intr - 50137 50095 43 0 1 110 99 -6 0.146 0.02 3.03 Intr - 55795 55609 187 2 1 103 110 156 0.457 17.13 3.02 Intr - 73219 73052 168 2 0 45 86 134 0.705 7.90 3.01 Init - 76590 76536 55 0 1 56 101 30 0.577 2.61 3.00 Prom - 86332 86293 40 -5.65 4.00 Prom + 87277 87316 40 -9.55 4.01 Init + 89128 89317 190 0 1 48 47 109 0.093 2.02 4.02 Intr + 91203 91363 161 1 2 58 54 129 0.096 5.29 4.03 Intr + 101426 101500 75 1 0 35 67 125 0.396 3.89 4.04 Intr + 103835 103972 138 1 0 54 47 123 0.406 4.54 4.05 Intr + 104507 104641 135 1 0 27 72 119 0.793 4.04 4.06 Term + 105080 105160 81 1 0 102 42 69 0.499 0.41 4.07 PlyA + 106417 106422 6 1.05 5.04 PlyA - 106623 106618 6 1.05 5.03 Term - 107626 107550 77 2 2 43 42 91 0.060 -2.98 5.02 Intr - 115723 115483 241 1 1 92 105 128 0.489 11.10 5.01 Init - 121267 121031 237 1 0 95 53 109 0.593 6.16 5.00 Prom - 121616 121577 40 -2.95 6.00 Prom + 123065 123104 40 -2.95 6.01 Init + 128205 128229 25 2 1 76 98 34 0.631 2.94 6.02 Intr + 134254 134811 558 2 0 34 20 376 0.847 17.27 6.03 Intr + 134869 135317 449 2 2 89 80 230 0.606 14.44 6.04 Term + 139985 140104 120 1 0 29 43 124 0.602 -0.51 6.05 PlyA + 140999 141004 6 1.05 7.03 PlyA - 141554 141549 6 1.05 7.02 Term - 144734 144632 103 1 1 76 52 132 0.872 5.17 7.01 Intr - 145980 145789 192 2 0 87 25 116 0.647 2.89 Click here to view a PDF image of the predicted gene(s) Click here for a PostScript image of the predicted gene(s) Predicted peptide sequence(s): >AC010727|GENSCAN_predicted_peptide_1|197_aa MLFLGLTTTFLLVFFLHAGLYTALTAARLLDESSMRTGSLSLLPPALSSASGTVPGTQHI YLSPWQEGLCLFYTMTTALAKLTRGIQWPNPTFPIDALMNEQQFEDEHIGCSFCVTLPAL QSSGSPEEWEIPEAPTDLPATQSPGPHVHCSLFGGLPDLSEHSRIPKDTPSSFPSQLQVP SGSPCSSAPATSAGNTH >AC010727|GENSCAN_predicted_peptide_2|156_aa MTTPPLADARVVVSEDLQCHTGALPVNGCEVVEFPPGDHLLLALLNGCSFELCCITGMSM WWCFGKQPLCAEMAGSQKTDLHNEGYILELDCCSSLDHPTDQKLIPEFIKKVSATVRVPL KRFASVPLSEQAAGVGLTADRPAGLRRDSFQTLMVM >AC010727|GENSCAN_predicted_peptide_3|198_aa MNKTKSLPSLGDTPENCDGLRLHLASCWLAYKPANEAESHGCTGECPIRLRVQHAGASTM CPGRNEGFGGSDVVAELPGSSAVRLASLRDLPAQLLELYQQGFSLAALHPFVQPTHEREK TPLEHIFRAILIKKTDRTRSDCFSNSEPQGEENEAEEVINDFSVRSLEWLRRVPSFLSNF VSHYSAPVTAEPTVTDSF >AC010727|GENSCAN_predicted_peptide_4|259_aa MQPPPSQTAPVPSTTFHTGPTHRTWPRLPPLPATHPHLYWIQTLDSFVILKHAKHMLTPE GAFCWTNLGHWLEEWRRQADTKRQCKGRCTTFHRCLVQAGRNWRRKVGLQDAALGSMALK VQPVGISSPDESTHPQEARLIEESGHQQVMGSMTLNVAPDPQRLCGSQAAARHTGHMESE DLSASCGSQHPSPLLHHNTSAAGIPREAREGAGAEQPFCHEDEGLYEEEPPTGFTMLGSP VKHPPPSQNLQAPAARYPS >AC010727|GENSCAN_predicted_peptide_5|184_aa MACWQDGQQVGPGCRFLSTWAPLWGYLSFLMAWWLDSTSRHPKSQGVNALKFLKAGSRNW NCVIPSYSIGQAILEPRFRENYGIFYTRVFKDKISEKNHLPAFFLWPAAAEMGCGLNKLE KRDEKRPGNIYSTLKRPQVETKIDVSYEYRFLEFTTLSAAYPPHMKESSAPTASPLKGVA VIFV >AC010727|GENSCAN_predicted_peptide_6|383_aa MTAGNPDDRITHNHTGQLRASTESQTDSRERAWFYNLGLHAVLAAKGSDTGGEGHQPWPR TGVRVPDNGASRNTQPPPPRPDPYPSWRPLSLEKWVKTREENPLQKRRSPFSVLGTLGTR VPVLLVVPALPRSARHVCSYTSSTSVPRANPPPRAHAEGQSGTASTLPHPGRAHPGNLDS ATSSLLFFSEDPSGSASEPAEPTTPNRLLRSASPYIAPGMTPLHPFPAKKSWPKIQASQG SAQVPIRRGPGGAALAAPEPRQRATCREAPRAVPTTRQRPGPWGQTEGPVPVDTPSPVAP PPPPTVWDRRRRRRGRGLPEAACSVPGGKFVPYRGGGAGRTWAPYLRTLWTAVQLLGFVQ EEHYRPSVEEGNRLFILPIDAGQ >AC010727|GENSCAN_predicted_peptide_7|98_aa XSESQVLATAVDSPVDEHMWKSCGFPVFHHQEMGQHGHLWVIIVIPPIPRSAYLPGICGT PLAVCPAAPSPSAMIENFLRPSPEADASAMLPVQPSEP Explanation Gn.Ex : gene number, exon number (for reference) Type : Init = Initial exon (ATG to 5' splice site) Intr = Internal exon (3' splice site to 5' splice site) Term = Terminal exon (3' splice site to stop codon) Sngl = Single-exon gene (ATG to stop) Prom = Promoter (TATA box / initation site) PlyA = poly-A signal (consensus: AATAAA) S : DNA strand (+ = input strand; - = opposite strand) Begin : beginning of exon or signal (numbered on input strand) End : end point of exon or signal (numbered on input strand) Len : length of exon or signal (bp) Fr : reading frame (a forward strand codon ending at x has frame x mod 3) Ph : net phase of exon (exon length modulo 3) I/Ac : initiation signal or 3' splice site score (tenth bit units) Do/T : 5' splice site or termination signal score (tenth bit units) CodRg : coding region score (tenth bit units) P : probability of exon (sum over all parses containing exon) Tscr : exon score (depends on length, I/Ac, Do/T and CodRg scores) Comments The SCORE of a predicted feature (e.g., exon or splice site) is a log-odds measure of the quality of the feature based on local sequence properties. For example, a predicted 5' splice site with score > 100 is strong; 50-100 is moderate; 0-50 is weak; and below 0 is poor (more than likely not a real donor site). The PROBABILITY of a predicted exon is the estimated probability under GENSCAN's model of genomic sequence structure that the exon is correct. This probability depends in general on global as well as local sequence properties, e.g., it depends on how well the exon fits with neighboring exons. It has been shown that predicted exons with higher probabilities are more likely to be correct than those with lower probabilities. Resultats en format txt de la seqüència emmascarada: GENSCANW output for sequence 11:13:35 GENSCAN 1.0 Date run: 28-Feb-102 Time: 11:13:44 Sequence 11:13:35 : 148061 bp : 42.63% C+G : Isochore 1 ( 0 - 43 C+G%) Parameter matrix: HumanIso.smat Predicted genes/exons: Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr.. ----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------ 1.01 Init + 9671 9757 87 1 0 56 30 149 0.074 4.59 1.02 Term + 13863 14018 156 2 0 96 37 72 0.063 -0.15 1.03 PlyA + 14461 14466 6 1.05 2.11 PlyA - 15337 15332 6 1.05 2.10 Term - 20418 20316 103 2 1 50 50 126 0.492 1.77 2.09 Intr - 20698 20620 79 2 1 102 86 40 0.493 2.89 2.08 Intr - 31241 31069 173 1 2 122 70 117 0.623 11.96 2.07 Intr - 32642 32473 170 2 2 89 57 122 0.957 7.02 2.06 Intr - 39494 39360 135 2 0 38 88 64 0.027 1.24 2.05 Intr - 46079 46026 54 2 0 92 30 100 0.187 2.86 2.04 Intr - 50137 50095 43 0 1 110 99 -6 0.288 0.02 2.03 Intr - 55795 55609 187 2 1 103 110 156 0.661 17.13 2.02 Intr - 73219 73052 168 2 0 45 86 134 0.539 7.90 2.01 Init - 75796 75712 85 1 1 85 44 33 0.427 -0.32 2.00 Prom - 86332 86293 40 -5.65 3.00 Prom + 87277 87316 40 -9.55 3.01 Init + 89128 89317 190 0 1 48 47 109 0.244 2.02 3.02 Intr + 91203 91363 161 1 2 58 54 129 0.613 5.29 3.03 Intr + 101426 101500 75 1 0 35 67 125 0.625 3.89 3.04 Intr + 103835 103972 138 1 0 54 47 123 0.629 4.54 3.05 Intr + 104507 104641 135 1 0 27 72 119 0.798 4.04 3.06 Intr + 105080 105147 68 1 2 102 68 68 0.257 2.98 3.07 Term + 115385 115523 139 2 1 102 48 111 0.256 4.95 3.08 PlyA + 115567 115572 6 1.05 4.00 Prom + 123065 123104 40 -2.95 4.01 Init + 130094 130211 118 1 1 58 91 25 0.673 0.26 4.02 Intr + 134254 134811 558 2 0 34 20 376 0.847 17.27 4.03 Intr + 134869 135317 449 2 2 89 80 230 0.634 14.44 4.04 Term + 139985 140104 120 1 0 29 43 124 0.672 -0.51 4.05 PlyA + 141413 141418 6 1.05 5.02 PlyA - 141554 141549 6 1.05 5.01 Term - 145980 145776 205 2 1 87 47 115 0.520 3.16 Click here to view a PDF image of the predicted gene(s) Click here for a PostScript image of the predicted gene(s) Predicted peptide sequence(s): >11:13:35|GENSCAN_predicted_peptide_1|80_aa MLFLGLTTTFLLVFFLHAGLYTALTAARLCPLAIFSPADSNPNGTWLFEWEFSQRQKRGK PYVGCNSVTLILKTLRKDFT >11:13:35|GENSCAN_predicted_peptide_2|398_aa MSCSPLALTFVFCLPFLHCGKEVLGYGEGLRLHLASCWLAYKPANEAESHGCTGECPIRL RVQHAGASTMCPGRNEGFGGSDVVAELPGSSAVRLASLRDLPAQLLELYQQGFSLAALHP FVQPTHEREKTPLEHIFRAILIKKTDRTRSDCFSNSEPQGEENEAEEVINDFSVRSLEWK ESFTRCPWAFEFTALVWPSDPSPAWRMTTPPLADARVVVSEDLQCHTGALPVNGCEVVEF PPGDHLLLALLNGCSFELCCITGMSMWWCFGKQPLCAEMAGSQKTDLHNEGYILELDCCS SLDHPTDQKLIPEFIKKVSATVRVPLKRFASVPLSEQAEKLPHIQGSKTILELVQEADDQ RRSRRPTWELLKVRGEQLLLPDLAVAMGHLDVTLSLPY >11:13:35|GENSCAN_predicted_peptide_3|301_aa MQPPPSQTAPVPSTTFHTGPTHRTWPRLPPLPATHPHLYWIQTLDSFVILKHAKHMLTPE GAFCWTNLGHWLEEWRRQADTKRQCKGRCTTFHRCLVQAGRNWRRKVGLQDAALGSMALK VQPVGISSPDESTHPQEARLIEESGHQQVMGSMTLNVAPDPQRLCGSQAAARHTGHMESE DLSASCGSQHPSPLLHHNTSAAGIPREAREGAGAEQPFCHEDEGLYEEEPPTGFTMLGSP VKHPPPSQNLQAPAASPHPIQLSNCTPIPCRPLLETPPAPIHSHPMYSPALRVVNSRKRY S >11:13:35|GENSCAN_predicted_peptide_4|414_aa MVSPPSQSWQGAITAECHLIFQLFGSGPLRVIFPPEFSPGITHNHTGQLRASTESQTDSR ERAWFYNLGLHAVLAAKGSDTGGEGHQPWPRTGVRVPDNGASRNTQPPPPRPDPYPSWRP LSLEKWVKTREENPLQKRRSPFSVLGTLGTRVPVLLVVPALPRSARHVCSYTSSTSVPRA NPPPRAHAEGQSGTASTLPHPGRAHPGNLDSATSSLLFFSEDPSGSASEPAEPTTPNRLL RSASPYIAPGMTPLHPFPAKKSWPKIQASQGSAQVPIRRGPGGAALAAPEPRQRATCREA PRAVPTTRQRPGPWGQTEGPVPVDTPSPVAPPPPPTVWDRRRRRRGRGLPEAACSVPGGK FVPYRGGGAGRTWAPYLRTLWTAVQLLGFVQEEHYRPSVEEGNRLFILPIDAGQ >11:13:35|GENSCAN_predicted_peptide_5|68_aa XSESQVLATAVDSPVDEHMWKSCGFPVFHHQEMGQHGHLWVIIVIPPIPRSAYLPGICGT PLAVWSGK Explanation Gn.Ex : gene number, exon number (for reference) Type : Init = Initial exon (ATG to 5' splice site) Intr = Internal exon (3' splice site to 5' splice site) Term = Terminal exon (3' splice site to stop codon) Sngl = Single-exon gene (ATG to stop) Prom = Promoter (TATA box / initation site) PlyA = poly-A signal (consensus: AATAAA) S : DNA strand (+ = input strand; - = opposite strand) Begin : beginning of exon or signal (numbered on input strand) End : end point of exon or signal (numbered on input strand) Len : length of exon or signal (bp) Fr : reading frame (a forward strand codon ending at x has frame x mod 3) Ph : net phase of exon (exon length modulo 3) I/Ac : initiation signal or 3' splice site score (tenth bit units) Do/T : 5' splice site or termination signal score (tenth bit units) CodRg : coding region score (tenth bit units) P : probability of exon (sum over all parses containing exon) Tscr : exon score (depends on length, I/Ac, Do/T and CodRg scores) Comments The SCORE of a predicted feature (e.g., exon or splice site) is a log-odds measure of the quality of the feature based on local sequence properties. For example, a predicted 5' splice site with score > 100 is strong; 50-100 is moderate; 0-50 is weak; and below 0 is poor (more than likely not a real donor site). The PROBABILITY of a predicted exon is the estimated probability under GENSCAN's model of genomic sequence structure that the exon is correct. This probability depends in general on global as well as local sequence properties, e.g., it depends on how well the exon fits with neighboring exons. It has been shown that predicted exons with higher probabilities are more likely to be correct than those with lower probabilities.