LOCUS CDK7_MOUSE 346 aa linear ROD 20-AUG-2001
DEFINITION CELL DIVISION PROTEIN KINASE 7 (CDK-ACTIVATING KINASE) (CAK) (39
KDA PROTEIN KINASE) (P39 MO15) (PROTEIN-TYROSINE KINASE MPK-7) (CR4
PROTEIN KINASE) (CRK4).
ACCESSION Q03147
PID g1705723
VERSION Q03147 GI:1705723
DBSOURCE swissprot: locus CDK7_MOUSE, accession Q03147;
class: standard.
created: Oct 1, 1994.
sequence updated: Oct 1, 1996.
annotation updated: Aug 20, 2001.
xrefs: gi: gi: 562023, gi: gi: 562024, gi: gi: 395047, gi: gi:
395048, gi: gi: 53199, gi: gi: 53200, gi: gi: 296750, gi: gi:
296751
xrefs (non-sequence databases): HSSP Q63450, MGD MGI:102956,
InterPro IPR000719, InterPro IPR002290, Pfam PF00069, PROSITE
PS00107, PROSITE PS00108, PROSITE PS50011
KEYWORDS Transferase; Serine/threonine-protein kinase; ATP-binding;
Phosphorylation; Cell cycle; Cell division; Nuclear protein;
Transcription regulation.
SOURCE house mouse.
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Rodentia; Sciurognathi; Muridae; Murinae; Mus.
REFERENCE 1 (residues 1 to 346)
AUTHORS Matsuoka,M., Kato,J.Y., Fisher,R.P., Morgan,D.O. and Sherr,C.J.
TITLE Activation of cyclin-dependent kinase 4 (cdk4) by mouse
MO15-associated kinase
JOURNAL Molecular and cellular biology. 14 (11), 7265-7275 (1994)
MEDLINE 95021255
PUBMED 7935441
REMARK SEQUENCE FROM N.A.
TISSUE=Macrophage
REFERENCE 2 (residues 1 to 346)
AUTHORS Stepanova,L.Yu., Ershler,M.A. and Belyavsky,A.V.
TITLE Sequence of the cDNA encoding murine CRK4 protein kinase
JOURNAL Gene. 149 (2), 321-324 (1994)
MEDLINE 95047496
PUBMED 7959010
REMARK SEQUENCE FROM N.A.
STRAIN=CBA; TISSUE=Bone marrow
REFERENCE 3 (residues 1 to 346)
AUTHORS Gilardi-Hebenstreit,P., Nieto,M.A., Frain,M., Mattei,M.G.,
Chestier,A., Wilkinson,D.G. and Charnay,P.
TITLE An Eph-related receptor protein tyrosine kinase gene segmentally
expressed in the developing mouse hindbrain
JOURNAL Oncogene. 7 (12), 2499-2506 (1992)
MEDLINE 93096484
PUBMED 1281307
REMARK SEQUENCE OF 139-154 FROM N.A.
STRAIN=C57BL; TISSUE=Embryonic brain
REFERENCE 4 (residues 1 to 346)
AUTHORS Ershler,M., Nagorskaya,T.V., Visser,J.W. and Belyavsky,A.V.
TITLE Novel CDC2-related protein kinases produced in murine hematopoietic
stem cells
JOURNAL Gene. 124 (2), 305-306 (1993)
MEDLINE 93185941
PUBMED 8444355
REMARK SEQUENCE OF 141-176 FROM N.A.
STRAIN=CBA; TISSUE=Bone marrow
REFERENCE 5 (residues 1 to 346)
AUTHORS Ershler,M.A., Nagorskaya,T.V., Visser,J.W.M. and Belyavsky,A.V.
TITLE Identification of new protein kinase genes, similar to kinases of
the cdc2 family and expressed in murine hematopoietic stem cells
JOURNAL Dokl. Akad. Nauk SSSR 324, 893-897 (1992)
REMARK SEQUENCE OF 141-176 FROM N.A.
STRAIN=CBA; TISSUE=Bone marrow
COMMENT On Dec 4, 1996 this sequence version replaced gi:585510.
-------------------------------------------------------------------
This SWISS-PROT entry is copyright. It is produced through a
collaboration between the Swiss Institute of Bioinformatics and
the EMBL outstation - the European Bioinformatics Institute.
The original entry is available from http://www.expasy.ch/sprot
and http://www.ebi.ac.uk/sprot
------------------------------------------------------------------.
[FUNCTION] CATALYTIC PART OF CAK WHICH ACTIVATES CYCLIN-ASSOCIATED
CDC2/CDK2/CDK4 BY THREONINE PHOSPHORYLATION. ITS EXPRESSION AND
ACTIVITY ARE CONSTANT THROUGHOUT THE CELL CYCLE. CAK IS TIGHTLY
ASSOCIATED WITH A MULTIPROTEIN COMPLEX TFIIH, WHICH PLAYS A DUAL
ROLE IN TRANSCRIPTION AND DNA REPAIR.
[ENZYME REGULATION] PHOSPHORYLATION AT T-170 IS REQUIRED FOR
ENZYMATIC ACTIVITY (BY SIMILARITY).
[SUBUNIT] MAMMALIAN CAK CONTAINS THREE COMPONENTS: CDK7, CYCLIN H,
AND AN ASSEMBLY FACTOR CALLED MAT1.
[SUBCELLULAR LOCATION] NUCLEAR (BY SIMILARITY).
[SIMILARITY] BELONGS TO THE SER/THR FAMILY OF PROTEIN KINASES.
CDC2/CDKX SUBFAMILY.
FEATURES Location/Qualifiers
source 1..346
/organism="Mus musculus"
/db_xref="taxon:10090"
1..346
Protein 1..346
/product="CELL DIVISION PROTEIN KINASE 7"
/EC_number="2.7.1.-"
Region 11..12
/region_name="Conflict"
/note="RY -> HN (IN REF. 2)."
Region 12..295
/region_name="Domain"
/note="PROTEIN KINASE."
Site 18..26
/site_type="np-binding"
/note="ATP (BY SIMILARITY)."
Region 20
/region_name="Conflict"
/note="E -> R (IN REF. 2)."
Site 41
/site_type="binding"
/note="ATP (BY SIMILARITY)."
Region 100
/region_name="Conflict"
/note="V -> L (IN REF. 2)."
Region 104
/region_name="Conflict"
/note="D -> H (IN REF. 2)."
Region 130..131
/region_name="Conflict"
/note="QH -> HN (IN REF. 2)."
Site 137
/site_type="active"
/note="BY SIMILARITY."
Site 164
/site_type="phosphorylation"
/note="(BY SIMILARITY)."
Site 170
/site_type="phosphorylation"
/note="(BY SIMILARITY)."
ORIGIN
1 mavdvksrak ryekldflge gqfatvykar dkntnqivai kkiklghrse akdginrtal
61 reikllqels hpniigllda fghksnislv fdfmetdlev iikdnslvlt pshikaymlm
121 tlqgleylhq hwilhrdlkp nnllldengv lkladfglak sfgspnrayt hqvvtrwyra
181 pellfgarmy gvgvdmwavg cilaelllrv pflpgdsdld qltrifetlg tpteeqwpdm
241 cslpdyvtfk sfpgvplqhi fiaagddlle liqglflfnp ctrttasqal ktkyfsnrpg
301 ptpgcqlprp ncpvealkep anptvatkrk raealeqgil pkklif
//
Revised: October 24, 2001.
