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CLUSTAL W (1.81) Multiple Sequence Alignments Sequence format is Pearson Sequence 1: BOVINE 424 aa Sequence 2: MURINE 434 aa Sequence 3: INFLUENZA 654 aa Sequence 4: RAT 439 aa Sequence 5: PUFFINOSIS 439 aa Sequence 6: BREDA 416 aa Sequence 7: BERNE 142 aa Sequence 8: TOROVIRUS 416 aa Sequence 9: CORONAVIRUS 424 aa Sequence 10: MURINE2 97 aa Start of Pairwise alignments Aligning... Sequences (1:2) Aligned. Score: 56 Sequences (4:5) Aligned. Score: 85 Sequences (2:3) Aligned. Score: 18 Sequences (3:4) Aligned. Score: 18 Sequences (1:3) Aligned. Score: 20 Sequences (4:6) Aligned. Score: 27 Sequences (4:7) Aligned. Score: 27 Sequences (2:4) Aligned. Score: 90 Sequences (3:5) Aligned. Score: 18 Sequences (1:4) Aligned. Score: 59 Sequences (4:8) Aligned. Score: 22 Sequences (3:6) Aligned. Score: 27 Sequences (2:5) Aligned. Score: 85 Sequences (3:7) Aligned. Score: 25 Sequences (1:5) Aligned. Score: 58 Sequences (4:9) Aligned. Score: 59 Sequences (4:10) Aligned. Score: 72 Sequences (2:6) Aligned. Score: 23 Sequences (3:8) Aligned. Score: 21 Sequences (2:7) Aligned. Score: 26 Sequences (1:6) Aligned. Score: 28 Sequences (1:7) Aligned. Score: 27 Sequences (5:6) Aligned. Score: 28 Sequences (3:9) Aligned. Score: 20 Sequences (2:8) Aligned. Score: 22 Sequences (5:7) Aligned. Score: 25 Sequences (3:10) Aligned. Score: 7 Sequences (6:7) Aligned. Score: 73 Sequences (1:8) Aligned. Score: 23 Sequences (2:9) Aligned. Score: 56 Sequences (5:8) Aligned. Score: 22 Sequences (2:10) Aligned. Score: 72 Sequences (6:8) Aligned. Score: 76 Sequences (7:8) Aligned. Score: 87 Sequences (1:9) Aligned. Score: 95 Sequences (7:9) Aligned. Score: 28 Sequences (7:10) Aligned. Score: 4 Sequences (1:10) Aligned. Score: 57 Sequences (9:10) Aligned. Score: 62 Sequences (5:9) Aligned. Score: 58 Sequences (5:10) Aligned. Score: 69 Sequences (6:9) Aligned. Score: 27 Sequences (6:10) Aligned. Score: 26 Sequences (8:9) Aligned. Score: 25 Sequences (8:10) Aligned. Score: 17 Guide tree file created: [/net/nfs0/vol1/production/w3nobody/tmp/511511.157466-152331.dnd] Start of Multiple Alignment There are 9 groups Aligning... Group 1: Sequences: 2 Score:9177 Group 2: Sequences: 2 Score:9179 Group 3: Sequences: 3 Score:9025 Group 4: Sequences: 4 Score:1881 Group 5: Sequences: 6 Score:5413 Group 6: Sequences: 2 Score:2896 Group 7: Sequences: 3 Score:5399 Group 8: Sequences: 9 Score:2003 Group 9: Delayed Sequence:3 Score:2732 Alignment Score 33127 CLUSTAL-Alignment file created [/net/nfs0/vol1/production/w3nobody/tmp/511511.157466-152331.aln]
CLUSTAL W (1.81) multiple sequence alignment BOVINE ---------MFLLPR--FVLVSCIIGSLGFDNPPTNVVSHLNGDWFLFGDSRSDCNHVVN 49 CORONAVIRUS ---------MFLLPR--FILVSCIIGSLGFYNPPTNVVSHVNGDWFLFGDSRSDCNHIVN 49 MURINE ----MGCMCIAMAPRTLLLLIGCQL-VFGF-NEPLNIVSHLNDDWFLFGDSRSDCTYVEN 54 RAT ----MGRMCIAMAPRTLLLLIGCQL-VFGF-NEPINIVSHLNDDWFLFGDSRSDCTYVEN 54 PUFFINOSIS ----MGSMCIAMAPRTLLLLIGCQL-ALGF-NEPLNVVSHLSDDWFLFGDSRSDCSYVEN 54 MURINE2 -------MCMAMAPRTLLLLIGCQL-VFGF-NEPLNTVSHSNDDWFLFGDSRSDCNHISS 51 BERNE ------------------------------------------------------------ TOROVIRUS --------------MLSLILFFPSFAFAVTPVTPYFGPLYITFNCCLFGDSRSDCTKVQS 46 BREDA --------------MLSLILFFPSFAFAATPVTPYYGPGHITFDWCGFGDSRSDCTNPQS 46 INFLUENZA MFFSLLLMLGLTEAEKIKICLQKQVNSSFSLHNGFGGNLYATEEKRMFELVKPKAGASVL 60 BOVINE TNPRNYSYMDLNPA------------LCDSGKISSKAGNSIFRSFHFTD-----FYNYTG 92 CORONAVIRUS INPHNYSYMDLNPV------------LCDSGKISSKAGNSIFRSFHFTD-----FYNYTG 92 MURINE NGHPKLDWLDLDPK------------LCNSGRIYAKSGNSLFRSFHFID-----FYNYSG 97 RAT NGHPKLDWLDLDPK------------LCNSGKIAAKSGNSLFRSFHFTD-----FYNYTG 97 PUFFINOSIS NGHPAFDWLDLPQE------------LCHSGKISAKSGNSLFKSFHFTD-----WYNYTG 97 MURINE2 LSQQNYKYMDINPE------------LCKSGKISSKAGNSLFRSFHFTD-----FYNYSG 94 BERNE ------------------------------------------------------------ TOROVIRUS PMS-----LDNPQN------------FCP--NFSLKSSSSMFFSIHYNNHSSLVLFDNFN 87 BREDA PMS-----LDIPQQ------------LCP--KFSSKSSSSMFLSLHWNNHSSFVSYDYFN 87 INFLUENZA NQSTWIGFGDSRTDQSNSAFPRSLMSAKTADKFRSLSGGSLMLSMFGPPGK--VDYLYQG 118 BOVINE EGQQIIFYEGINFTPYHAFKCTTSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVY 152 CORONAVIRUS EGQQIIFYEGVNFTPYHAFKCNRSGSNDIWMQNKGLFYTQVYKNMAVYRSLTFVNVPYVY 152 MURINE EGDQVIFYEGVNFSPSHGFKCLAYGDNKRWMGNKARFYARVYEKMAQYRSLSFVNVSYAY 157 RAT EGDQIIFYEGVSFSPSHGFKCLVEGDNNKWMGNKARFYALLYKKMAQYRSLSFVTVPYAY 157 PUFFINOSIS EGDQVIFYEGVNFSPSHGFKCLAEGDNKRWMGNKARFYALVYKKMAYYRSLSFVNVSYSY 157 MURINE2 EGS--------------------------------------------------------- 97 BERNE ------------------------------------------------------------ TOROVIRUS CRIEKVYYNGVNLSPRNQYSCYDEG-VDSYMELKTSFNIKLNQMATILRCIKLIQLKARS 146 BREDA CGVEKVFYEGVNFSPRKQYSCWDEG-VDGWIELKTRFYTKLYQMATTSRCIKLIQLQAPS 146 INFLUENZA CGKHKVFYEGVNWSPHAAIDCYRKN----WTDIKLNFQKSIYELASQSHCMSLVNALDKT 174 BOVINE NGSAQSTALCK--------SGSLVLNNPAYIAREANF-GDYYYKVEADFYLSGCDEYIVP 203 CORONAVIRUS NGSAQSTALCK--------SGSLVLNNPAYIAPQANS-GDYYYKVEADFYLSGCDEYIVP 203 MURINE GGNAKPTSICK--------DKTLTLNNPTFISKESNY-VDYYYESEANFTLQGCDEFIVT 208 RAT GGNAKPTSICK--------DKTLTLNNPTFISKESNY-VDYYYVSEANFTLQGCDEFIVP 208 PUFFINOSIS GGKAKPTAICK--------DNTLTLNNPTFISKESNY-VDYYYESDANFTLEGCDEFIVP 208 MURINE2 ------------------------------------------------------------ BERNE ------------------------------------------------------------ TOROVIRUS SFTTLQDVVCR--------TNKYLPNNPTFALLSDTVPTWVQFVLPDLSGKTICIKYLVP 198 BREDA SLPTLQAGVCR--------TNKQLPDNPRLALLSDTVPTSVQFVLPGSSGTTICTKHLVP 198 INFLUENZA IPLQVTKGVAKNCNNSFLKNPALYTQEVKPLEQICGEENLAFFTLPTQFGTYECKLHLVA 234 BOVINE LCIFNG---KFLSN-----TKYYDDSQYYFNKDTGVIYGLNSTETIT-----TGFDFNCH 250 CORONAVIRUS LCIFNG---KFLSN-----TKYYDDSQYYFNKDTGVIYGLNSTETIT-----TGFDLNCY 250 MURINE LCVSNG---HSKSSSSDPANKYYTDAQSYYNMDTGVLYGFNSTLDVGNTVQNPGLDLTCR 265 RAT LCVFNG---HSRGSSSDPANKYYTDSQSYYNIDTGVLYGFNSTLDVGNTAQNPGLDLTCM 265 PUFFINOSIS LCVFNG---HSRGSSSDPANKYYMDSQMYYNMDTGVFYGFNSTLDVGNTAQNPGLDLTCI 265 MURINE2 ------------------------------------------------------------ BERNE ------------------------------------------------------------ TOROVIRUS FCHLNHGCFTAGSSCPPFGVSYVSDSFNYGFNDATPYIGLA-----------ESHDNVCD 247 BREDA FCYLNHGCFTTGGSCLPFGVSYVSDSFYYGYYDATPQIGST-----------ESHDYVCD 247 INFLUENZA SCYFIYD-----SKEVYNKRGCGNYFQVIYDSSGKVVGGLDNRVSPYTGNSGDTPTMQCD 289 BOVINE YLVLPSGNYLAISNELLLTVPTKAICLNKRKDFTPVQVVDSRWNNARQSDNMTAVACQ-P 309 CORONAVIRUS YLVLPSGNYLAISNELLLTVPTKAICLNKRKDFTPVQVVDSRWNNARQSDNMTAVACQ-P 309 MURINE YLALTPGNYKAVSLEYLLSLPSKAICLRKPKSFMPVQVVDSRWNSTRQSDNMTAVACQ-L 324 RAT YLVLTPGNYKAVSLEYLLTIPSKAICLRKPKRFMPVQVVDSRWNSTRQSDNMTAVACQ-L 324 PUFFINOSIS YYALTPGNYKAVSLEYLLTIPSKAICLRKPKRFMPVQVVDSRWNNAKHSDNMTAVACQ-T 324 MURINE2 ------------------------------------------------------------ BERNE -----------------------------MNFTVPVQAIQSIWSVGKESDDAIAEACK-P 30 TOROVIRUS YLFVEAGTHNASIVGNFLFYPTKSYCFNTMNFTVPVQAIQSIWSEGNESDDAIAEACK-P 306 BREDA YLFMEPGTYNASTVGKFLVYPTKSYCMDTMNITVPVQAVQSIWSEQYASDDAIGQACK-A 306 INFLUENZA MLQLKPGRYSVRSSPRFLLMPERSYCFD-MKEKGPVTAVQSIWGKGRKSDYAVDQACLST 348 BOVINE PYCYFRNSTTNYVG--VYDINHGDAGFTSILSGLLYDSPCFSQQGVFRYDN-VSSVWPLY 366 CORONAVIRUS PYCYFRNSTTNYVG--VYDINHGDAGFTSILSGLLYNSPCFSQQGVFRYDN-VSSVWPLY 366 MURINE PYCFFRNTSADYSGG-THDVHHGDFHFRQLLSGLLYNVSCIAQQGAFVYNN-VSSSWPAY 382 RAT PYCFFRNTSADYSGD-THDVHHGDFYFRQLLSGLLYNVSCIAQQGAFLYNN-VSSIWPVY 382 PUFFINOSIS PYCLFRNTSSGYNGS-THDVHHGGFHFRKLLSGLLYNVSCIAQQGAFFYNN-VSSQWPVL 382 MURINE2 ------------------------------------------------------------ BERNE PFCIYFSKKTPYTVTNGSNADHGDDEVRQMMRGLLYNSSCISAQGHTPLAL-YSTAMLYP 89 TOROVIRUS PFCIYYSKTTPYTVTNGSNADHRDDEVRMMVRGLLYNSSCISAQGSTPLAL-YSTAMLYA 365 BREDA PYCIFYNKTTPYTVTNGSDANHGDDEVRMMMQGLLRNSSCISPQGSTPLAL-YSTEMIYE 365 INFLUENZA PGCMLIQKQKPYIG--EADDHHGDQEMRELLSGLDYEARCISQSGWVNETSPFTEEYLLP 406 BOVINE P-YGRCPTAA------DINTPDVPICVYDPLPIILLGILLGVAVIIIVVLLLYFMVDNGT 419 CORONAVIRUS P-YGRCPTAA------DINNPDLPICVYDPLPVILLGILLGVAVIIIVVLLLYFMVDNGT 419 MURINE G-YGHCPTAA------NIG-YMAPVCIYEPLPVILLGVLLGIAVLIIVFLMFYFMTDSGV 434 RAT G-YGHCPTAA------NIG-YMAPVCLYDPLPVILLGVLLGIAVLIIVFLILYFMADSSV 434 PUFFINOSIS G-YGQCPTAA------NIE-FIAPVCLYDPLPVILLGVLLGIAVLIIVFLLFYFMTDSGV 434 MURINE2 ------------------------------------------------------------ BERNE PMYGSCPQYV------KLFDGSGS----ESVDVISSSYFVATWVLLVVVIILVFIIISFC 139 TOROVIRUS PIYGSCPQYV------KLFDTSGS----ESVDVISSSYFVATWVLLVVVVILIFVIISFF 415 BREDA PNYGSCPQFY------KLFDTSGN----ENIDVISSSYFVATWVLLVVVVILIFVIISFF 415 INFLUENZA PKFGRCPLAAKEESIPKIPDGLLIPTSGTDTTVTKPKSRIFGIDDLIIGLLFVAIVEAGI 466 BOVINE RLHDA------------------------------------------------------- 424 CORONAVIRUS RLHDA------------------------------------------------------- 424 MURINE ------------------------------------------------------------ RAT RLHEA------------------------------------------------------- 439 PUFFINOSIS RLHEA------------------------------------------------------- 439 MURINE2 ------------------------------------------------------------ BERNE ISN--------------------------------------------------------- 142 TOROVIRUS C----------------------------------------------------------- 416 BREDA C----------------------------------------------------------- 416 INFLUENZA GGYLLGSRKESGGGVTKESAEKGFEKIGNDIQILRSSTNIAIEKLNDRISHDEQAIRDLT 526 BOVINE ------------------------------------------------------------ CORONAVIRUS ------------------------------------------------------------ MURINE ------------------------------------------------------------ RAT ------------------------------------------------------------ PUFFINOSIS ------------------------------------------------------------ MURINE2 ------------------------------------------------------------ BERNE ------------------------------------------------------------ TOROVIRUS ------------------------------------------------------------ BREDA ------------------------------------------------------------ INFLUENZA LEIENARSEALLGELGIIRALLVGNISIGLQESLWELASEITNRAGDLAVEVSPGCWIID 586 BOVINE ------------------------------------------------------------ CORONAVIRUS ------------------------------------------------------------ MURINE ------------------------------------------------------------ RAT ------------------------------------------------------------ PUFFINOSIS ------------------------------------------------------------ MURINE2 ------------------------------------------------------------ BERNE ------------------------------------------------------------ TOROVIRUS ------------------------------------------------------------ BREDA ------------------------------------------------------------ INFLUENZA NNICDQSCQNFIFKFNETAPVPTIPPLDTKIDLQSDPFYWGSSLGLAITAANLMAALVIS 646 BOVINE -------- CORONAVIRUS -------- MURINE -------- RAT -------- PUFFINOSIS -------- MURINE2 -------- BERNE -------- TOROVIRUS -------- BREDA -------- INFLUENZA GIAICRTK 654
( ( ( ( BOVINE:0.02874, CORONAVIRUS:0.01607) :0.16378, ( INFLUENZA:0.41115, ( BREDA:0.10571, ( BERNE:0.06009, TOROVIRUS:0.06667) :0.08371) :0.20736) :0.20437) :0.09018, MURINE2:0.16169) :0.05584, ( MURINE:0.05139, RAT:0.04308) :0.01952, PUFFINOSIS:0.07989);